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Citations for Thermo Fisher :
601 - 650 of 835 citations for Ebola Virus Envelope Glycoprotein GP1 Bundibugyo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2021Quote: Nucleic acids were extracted from purified virus samples either using the PureLink Viral RNA/DNA Mini Kit (Thermo Fisher Scientific) or by phenol/ether method combined with ethanol precipitation ...
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bioRxiv - Microbiology 2021Quote: ... VeroE6 cells were used for live virus titration of biological samples and were maintained in DMEM (#11965092, Thermo Scientific, USA) with 10% FBS.
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bioRxiv - Microbiology 2021Quote: ... Five μl RNA (150 µl elution volume) was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using 100ng total RNA compared to an RNA standard curve using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on a Quant Studio 3 (Applied Biosystems).
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan(tm) Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were based on (21 ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were used as described previously (45) ...
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bioRxiv - Cell Biology 2022Quote: ... 4T1 cells were transduced with cell-free virus-containing supernatants for 48 hours and selected with 100 µg/mL of neomycin (Gibco) for 96 hours.
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bioRxiv - Immunology 2022Quote: ... were pulsed for 1 hr with 10μg/ml influenza virus NP(366-374) peptide and were subsequently labeled for 10 min at 37°C with 5 μM CFSE (Invitrogen) (CFSEhigh ...
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bioRxiv - Immunology 2022Quote: ... The extracted RNA was then added to TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Waltham, MA) containing primers and a fluorescently labeled hydrolysis probe specific for mRNA from the nucleocapsid gene of SARS-CoV-2 (forward primer 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... The nCoV_IP2 primer-probe set was used in combination with TaqMan fast virus 1-step master mix (Thermo Fisher Scientific) to detect viral RNA ...
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bioRxiv - Cell Biology 2022Quote: ... Assays were performed in 24-well plates coated with Geltrex LDEV (lactose dehydrogenase elevating virus)-Free Reduced Growth Factor Basement Membrane Matrix (ThermoFisher) at 75 µl/cm2 according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... The resuspended virus was purified using a 30% (wt/vol) sucrose cushion at 100,000 × g (Sorvall Discovery 90SE, Thermo Fisher) for two- and-a-half hours at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Proteins from virus-infected cells were immunoprecipitated using protein G magnetic Dynabeads according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... 500 ng of RNA was reversely transcribed using random primers (Eurofins) and Moloney murine leukaemia virus reverse transcriptase (M-MLV, Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Heathy human donor PBMCs or skin fibroblasts were reprogrammed into iPSCs using the integration-free based Sendai virus (Cytotune 2.0 kit from Life Technologies). Low passage primary fibroblasts were used to increase reprogramming efficiency ...
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bioRxiv - Microbiology 2022Quote: ... The presence of engineered mutations was confirmed by Sanger sequencing following extraction of RNA from virus stocks with TRIzol (Invitrogen) and cDNA amplification using a OneStep RT-PCR Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2019Quote: vRNA was extracted from virus supernatant containing the same amount of PFU using PureLink Viral RNA/DNA Mini Kit (Invitrogen). vRNA was reverse transcribed with Uni12/13 specific primers using Superscript IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... filled with the infusate (virus particles suspended in a solution of phosphate-buffered saline (PBS) and Oregon Green 488 (Invitrogen)) into the centers of the craniotomies ...
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bioRxiv - Molecular Biology 2019Quote: iPSCs were generated by transducing control and FXS patient fibroblasts with Sendai virus from the Cytotune 2.0 Reprogramming Kit (Life Technologies) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: Fibroblasts isolated either from human fetal liver tissues or human adult skin after collagenase dissociation were reprogrammed into iPSCs with the integration-free based Sendai virus (Cytotune 2.0 kit from Life Technologies). Fibroblasts were used at low population doubling (less than 5 ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription was performed to convert RNA into cDNA using the Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA samples were reverse transcribed (1µg of total RNA/sample) using the Moloney Murine Leukemia Virus (MML-V) enzyme (Life Technologies) and Oligo-dT Primers ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed as previously described(42) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2021Quote: ... that co-localises with IBDV virus factories (16) and a goat anti-mouse secondary antibody conjugated to Alexa-Fluor 488 (Invitrogen). Wells were scored as positive or negative based on the presence of infected cells ...
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bioRxiv - Immunology 2021Quote: ... 25 μl of virus was immediately mixed with 25 μl of serially diluted (2×) protein A/G purified IgG (ThermoFisher) from mouse sera (starting at 500 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed as previously described [39] using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2021Quote: ... Ten-fold serial dilutions (1×10−1 to 1×10−6) of virus stocks were prepared in MEM (supplemented with 25 mM HEPES (Gibco), 2mM L-Glutamine (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... 1µg was used for cDNA synthesis using a Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (SuperScript II from ThermoFisher Scientific) and random primers ...
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bioRxiv - Pathology 2021Quote: ... 10 µl of rSARS-CoV-2 NL virus stock was diluted in 10 ml of OptiMEM media (ThermoFisher Scientific, #51985091) and then added to cells ...
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bioRxiv - Microbiology 2019Quote: ... 200 ng of RNA from each sample was used to generate virus cDNA using in a Maxima H minus strand kit (Invitrogen) and ZIKV specific primers (Supplemental Table 6) ...
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bioRxiv - Immunology 2021Quote: ... dilutions of IgG were co-incubated with the virus (1000-2000 TCID50) for 1 h at 37°C prior to addition of VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Microbiology 2020Quote: ... Vero FM cells seeded in 24-well plates were infected in duplicate with 100 μl 2-fold serial dilutions of patient material in a total volume of 200 μl using virus isolation medium [DMEM (Gibco) containing ...
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bioRxiv - Immunology 2020Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of nucleocapsid (N ...
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bioRxiv - Microbiology 2021Quote: ... Primary cells were immortalized by transfection of pRSVAg1 plasmid expressing Simian Vacuolating Virus 40 large T antigen (SV40T) with lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco, Waltham, MA) to remove excess virus ...
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bioRxiv - Bioengineering 2021Quote: ... 21] or a Sendai virus reprogramming kit (Cyto Tune 2.0 Sendai reprogramming vectors, Ref#A16517; Lot#A16517, Invitrogen, Carlsbad, CA). hiPSCs were cultured on hESC-qualified Matrigel® (Corning ...
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bioRxiv - Microbiology 2021Quote: ... The attachment of HIV-1 virus produced in the presence of TMEM123 was also separately tested by pre-incubating the virus with mouse monoclonal anti-TMEM123 antibody (297617) (ThermoFisher) (25ug/ml ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 until the following day. Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat. no. 61965026 supplemented with 2% FBS (Gibco, Cat ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were validated free of virus mycoplasma contamination using the MycoSEQ assay (Thermo Fisher Scientific, Waltham, MA, USA) or STAT-Myco assay (IDEXX Bioanalytics) ...
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bioRxiv - Microbiology 2022Quote: Blood virus mixtures were titrated by ten-fold serial dilution in virus media (RPMI 1640 cell culture media supplemented with 5% fetal bovine serum [FBS] and 1% Penicillin-Streptomycin [Gibco]) in a 96-well plate before transferring dilutions to equivalent wells of a 96 well plate containing near confluent C6/36 cell monolayers ...
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bioRxiv - Neuroscience 2022Quote: Total RNA isolation from the AAV9 virus injected region of the left frontal cortex was performed as previously described [40] by TRIzol LS Reagent (10296010, Invitrogen). For cDNA synthesis ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Virus propagation and titration was performed in LLC-MK2 cells using Opti-MEM supplemented with 1.6% TrypLE™ Express (Gibco) and 100 μg/ml CaCl2 (OptiMEMinf ...
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bioRxiv - Plant Biology 2022Quote: ... was transcribed following DNase I treatment using Moloney Murine Leukemia Virus reverse transcriptase and an oligo dT(18) primer according to the manufacturer’s protocol (ThermoFisher Scientific). qPCR analysis was carried out in a CFX96-C1000 96-well plate thermocycler (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 until the following day. Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat. no. 61965026 supplemented with 2% FBS (Gibco, Cat ...