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Citations for Thermo Fisher :
301 - 350 of 868 citations for Ebola Virus Envelope Glycoprotein GP1 Bundibugyo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... total RNA was reverse-transcribed with Moloney murine leukemia virus reverse transcriptase (Fisher Scientific) using random hexamers (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... prior to addition of PE243 virus in complete DMEM media +10 mM HEPES (Gibco), at a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: UKEi001-A was reprogrammed with a Sendai Virus (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). Expansion and cardiac differentiation of iPSC were performed as recently described1 ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-Influenza A Virus M1 (1:1,000; clone: GA2B; Cat#: MA1-80736; Invitrogen), mouse anti-Influenza A virus M2 (1:1,000 ...
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bioRxiv - Bioengineering 2022Quote: ... consisting of 5 μL TaqMan Fast Virus 1-Step Master Mix (cat# 4444432, Thermofisher), 1.2 μL forward primer (0.6 μm) ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-Influenza A virus M2 (1:1,000; clone: 14C2; Cat#: MA1-082; Invitrogen), mouse anti-GAPDH (1:1,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Virus (undiluted) or FluoSpheres (580/605, 0.04 µm, diluted to 20% in saline, Invitrogen) were loaded into freshly pulled glass micropipettes and injected using a Nanoject III pressure injector (Drummond Scientific Company ...
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bioRxiv - Microbiology 2022Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2022Quote: ... Virus amplification was carried out in Spodoptera frugiperda Sf9 cells (Geneart, Thermo Fisher Scientific) and was used to infect Trichoplusia ni Tnao38 cells (103 ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription was performed using Murine Moloney Leukemia Virus Reverse Transcriptase (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific) and real-time PCR was performed using TaqMan assays (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Cellular RNA was mixed with TaqMan Fast Virus 1-step Master Mix (Applied Biosystems), 10 mM forward and reverse primers and 2 mM probe ...
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bioRxiv - Immunology 2023Quote: ... medium containing virus isolates was incubated in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11995073) with 1:10000 Vybrant (V22887 ...
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bioRxiv - Immunology 2023Quote: ... Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887) for 20 mins at 37°C ...
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bioRxiv - Biochemistry 2022Quote: HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Strep-tag (NBP2-41073; Novusbio) or influenza virus hemagglutinin tag (2-2.2.14; Thermo Scientific). After washing with PBS with 1% BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... Yamanaka reprogramming factors were induced to iPSCs via Sendai virus using CytoTune2.0 (ThermoFisher, A16517). To establish nGFP+ iPSCs ...
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bioRxiv - Genetics 2023Quote: ... Virus particles were subsequently washed multiple times with PBS (pH 7.4, Thermo Fisher Scientific) and physical titers were measured using a Qubit 3.0 fluorometer and the Qubit dsDNA HS assay kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Virus pellets and cell lysates were dissolved in radioimmunoprecipitation assay (RIPA) buffer (Thermo Scientific) containing protease inhibitors ...
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bioRxiv - Neuroscience 2023Quote: ... reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). Real-time PCR was performed using TaqMan assays (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: IPS cells were derived from skin fibroblasts using Cytotune Sendai virus kit (Thermofisher, France) and cultured on MEF in KO- DMEM supplemented with KO-SR (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: Elephas maximus endothelial and epithelial cells were transduced with Sendai virus (Thermofisher Scientific, A16517) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and gene-specific probes and reverse primers as follows ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and N gene-specific probe and reverse primer as previously described47:
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bioRxiv - Microbiology 2023Quote: ... containing 5 µl of template and TaqMan Fast Virus 1-step mastermix (Applied Biosystems). Primer sequences and concentrations and thermal cycling conditions for SARS-CoV-2 nucleocapsid 1 gene were as previously described (13) ...
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bioRxiv - Molecular Biology 2024Quote: ... Abelson virus-transformed pre-B cells were maintained in DMEM (Thermo Fisher, 11960-077) supplemented with 10% FBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... renilla luciferase fused to herpes simplex virus thymidine kinase promoter (pTK-Renilla, Thermo Scientific), and the indicated RIPK ...
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bioRxiv - Biochemistry 2019Quote: ... lentivector plasmids were transfected into HEK293T cells together with packaging and envelope plasmids (psPAX2 and pMD2.G) using Lipofectamine 3000 (Thermo Fisher Scientific, L3000008). Three days after transfection ...
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bioRxiv - Immunology 2022Quote: ... 2.7 μg envelope plasmid pMD2G and 8 μg target plasmid encoding shRNA or lentiCRISPRv2 encoding sgRNA using Lipofectamine® 2000 (Thermo Fisher Scientific). Lentivirus supernatants were collected at 36 h ...
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bioRxiv - Cancer Biology 2024Quote: ... addgene #12260) and 0.5 µg VSV-G envelope plasmid (pMD2.G, addgene #12259) in 57 µL Opti-MEM (Invitrogen, Frankfurt am Main, Germany) and 6 µL FuGENE HD (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... of Iowa) was prepared by diluting virus stocks to 400 p.f.u./µL into OptiMEM (Gibco) followed by addition of CaCl2 to a final concentration of 10 µM ...
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bioRxiv - Microbiology 2020Quote: ... and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075).
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed using the TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems). Assays were conducted on a QuantStudio5 Q-PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... The assay was performed using the TaqMan® Fast Virus 1-Step Kit (Life Technologies) and kit manual ...
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bioRxiv - Molecular Biology 2020Quote: ... a 20μL monoplex reaction contained 5μL of 4x TaqMan Fast Virus Master Mix (Thermo Fisher), 0.6µL of each primer (0.3μM final concentration each) ...
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bioRxiv - Immunology 2019Quote: ... two-fold virus stock dilutions were prepared in U-shaped 96-well plates (Thermo Scientific). The same volume of a 1% human O+ red blood cell suspension (Innovative Research ...
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bioRxiv - Microbiology 2019Quote: ... Other antibodies were obtained from commercial vendors as follows: Anti-coxsackie virus B3 antibodies (ThermoFisher), Anti-adenovirus A5 antibodies (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... RAW264.7 cells were then transduced with virus and selected using hygromycin at 10mg/ml (Invitrogen) to select for cells containing the shRNA plasmid.
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bioRxiv - Microbiology 2021Quote: ... and the number of virus particles was determined using a Nanodrop 2000 (Thermo Fisher Scientific). The adenovirus stocks were aliquoted and stored at -80°C.
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bioRxiv - Neuroscience 2021Quote: ... using TaqMan Fast Virus 1-Step Master Mix (PN4453800, Applied biosystems, Thermo Fisher Scientific, USA). PrimerTime primers and FAM-dye coupled detection probes were used for detecting Clstn3 mRNA level ...
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bioRxiv - Neuroscience 2021Quote: ... The final buffer for the virus was: dPBS + 5% Glycerol + 0.001 Pluronic F-68 (Invitrogen). The AAV titre (1.71 × 1013 GC/ml ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqManTM Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... The qPCR for Sendai virus detection was performed using TaqMan Gene Expression Assays (Life Technologies). Quantitative RT-PCR was performed using SYBR® Green PCR Master Mix (Applied Biosystems™ ...
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bioRxiv - Cell Biology 2022Quote: Cell lines were generated using Sendai virus CytoTune-iPS 2.0 Sendai reprogramming kit (Thermo Fisher) for transfection of Yamanaka’s factors ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of purified virus was analyzed with the Quant-it PicoGreen dsDNA Assay (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... concentrated virus was run on a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies), which was then analyzed using the Pierce Silver Stain Kit (Pierce ...
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bioRxiv - Immunology 2022Quote: ... For these assays we used TaqMan Fast Virus 1- Step Master Mix (ThermoFisher, catalog # 4444432) and custom primers/probes targeting the E gene sgRNA (forward primer ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA was extracted from amplified virus stock using Trizol reagent (Invitrogen; Cat no. #15596018). Reverse transcription was carried out using in-house produced Moloney murine leukemia virus (MMLV ...