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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... at 6-7 days in vitro (DIV) or Lipofectamine 2000 (Thermofisher) 24/72 hours before the experiments ...
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bioRxiv - Cell Biology 2021Quote: ... iPSCs were passaged every 6-7 days with Versene (Life Technologies) at a 1:12 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... DCFH-DA (6-carboxy-2′,7′- dichlorodihydrofluorescein diacetate) was from Invitrogen. DMSO and Evans Blue Dye were purchased from Sigma Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... for the maturation step (82% Leibovitz15 Medium, 8% fetal bovine serum, 8% Tryptose Phosphate Broth, 1% P/S, 1% GlutaMAX® (all Gibco) and 0.06% Insulin (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight at 4 °C on rotation and for one additional hour with mixed Dynabead protein G and A (20:10 µl, Life Technologies). Beads were washed with TSE- 150 (0.1% SDS,1% Triton ...
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bioRxiv - Developmental Biology 2020Quote: ... 8% CO2 in ESC medium (Advanced DMEM/F12: Neurobasal (1:1) Medium (Gibco), supplemented with 2.5% mESC-grade fetal bovine serum (Corning) ...
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bioRxiv - Neuroscience 2021Quote: ... G-a-mIgG2b A647 and G-a-mIgG1 CF680 (1:200, ThermoFisher), diluted in 0.1% BSA/BPS ...
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bioRxiv - Neuroscience 2021Quote: ... G-a-mIgG2b A647 and G-a-mIgG1 CF680 (1:200, ThermoFisher), diluted in 0.1% BSA/BPS ...
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bioRxiv - Cell Biology 2021Quote: ... embryos at the 3-6 somite stage were embedded oriented laterally in 1% low-melt agarose (Invitrogen, Carlsbad, CA) and imaged under bright field (to determine yolk elongation by taking major and minor axis measurements ...
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bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... The luminescence was detected for 5-6h with a signal integration time of 2min using Varioskan Flash plate reader (Thermo Fisher Scientific). For determining the effects of chemical inhibitors ...
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bioRxiv - Neuroscience 2020Quote: ... DAPI (4’,6-diamidino-phenylindole, Invitrogen) was added to the secondary antibodies solution ...
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bioRxiv - Neuroscience 2023Quote: ... DAPI (4’,6-diamidino-phenylindole, Invitrogen) was added to the secondary antibody solution ...
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bioRxiv - Genetics 2022Quote: ... These pieces were transferred to the lower surface of a 25 cm2 culture flask (6-8 pieces per flask) which had been pre-moistened with 1-2 mL of AmnioMAX Complete Medium (Thermo Scientific, #11269016) supplemented with 1% penicillin/streptomycin (GenDepot ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells from 2-D or 3-D cultures were harvested from one well of a 6-well plate and dissociated with Accutase (Thermo Fisher #A1110501), washed three times with 1x PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 1ml of bone marrow was incubated in one well of 6-well plate with 3 ml StemPro™ MSC serum-free medium (Gibco, Thermo Scientific). Media was changed after every two days until it reached to confluency ...
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bioRxiv - Biochemistry 2023Quote: ... SDS-PAGE was carried out on either NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate gels (Invitrogen). Western blotting analysis was carried out according to standard protocols with the antibodies listed in Table S5.
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bioRxiv - Cell Biology 2020Quote: ... All cell lines except BxPC-3 and SK-BR-3 were maintained in high glucose (4.5 g L-1) Dulbecco’s Eagle Medium (DMEM, ThermoFisher Scientific). BxPC-3 cells were maintained in RPMI medium (ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... These selected colonies were grown in King’s B medium consisting of 20 g l-1 Bacto proteose peptone No.3 (Gibco), 1.5 g l-1 Potassium phosphate dibasic trihydrate (P5504 ...
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bioRxiv - Immunology 2023Quote: ... 20 ng ml-1 GM-CSF (Biozym) or X38-Ag8 supernatant (1%) and from day 5 on additionally with 10 ng ml-1 IL-4 (Life technologies). At day 7 ...
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bioRxiv - Genomics 2023Quote: ... plugs were washed 4 times in 1×Wash Buffer (BNG) and 5 times in 1× TE Buffer (ThermoFisher Scientific, Waltham, MA). Then ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis was measured by caspase-3/7 staining using CellEvent® Caspase-3/7 Green ReadyProbes® Reagent (ThermoFisher Scientific Inc., cat# R37111). CHLA20 and NGP cells were grown until confluence on 6-well plates with six days of treatment with DMSO or GSK591 ...
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bioRxiv - Cancer Biology 2024Quote: ... 48hrs after transfection the cells were fixed and stained for activated Caspase-3/7 using Apoptosis CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) according to manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... or NuPAGE 3-8% Tris-Acetate Gels (Invitrogen) and transferred to Immun-Blot PVDF membrane (Bio-rad ...
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bioRxiv - Cancer Biology 2020Quote: ... or 3-8% Tris-Acetate gels (Thermo Fisher) for gel electrophoresis depending on the protein sizes of interest ...
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bioRxiv - Biochemistry 2021Quote: ... or 3-8% NuPAGE Tris-Acetate (ThermoFisher Scientific) gels and then transferred to 0.22 μm nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2020Quote: ... or 3–8%Tris-Acetate (Thermo Fisher Scientific) gradient gels ...
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bioRxiv - Biophysics 2020Quote: ... NuPAGE Novex 3%-8% Tris-Acetate Gel (Invitrogen) was used ...
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bioRxiv - Immunology 2023Quote: ... or 3-8% TA gels (Thermo Fisher Scientific) in TA buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... or a 3-8% Tris Acetate gel (Invitrogen). Proteins were wet transferred onto a PDVF membrane (Millipore ...
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bioRxiv - Immunology 2024Quote: ... or NuPage Tris-Acetate 3-8% gels (Invitrogen), for detection of TIR-1 ...
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bioRxiv - Immunology 2024Quote: ... or NuPage Tris-Acetate 3-8% gels (Invitrogen), for detection of TIR-1::3xFLAG ...
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bioRxiv - Microbiology 2023Quote: ... The RNase T1 ladder was prepared by denaturing 0.4 pmol of 5′ labelled Aar with 8 µL of 1× sequencing buffer (Ambion) at 95°C for 1 min ...
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bioRxiv - Developmental Biology 2023Quote: ... we slowly injected 0.5-1 μL rAAV (about 1∼3×109 genome copy (GC)) mixed with Chicago Sky Blue dye (0.1%, Fisher Scientific, Cat # AAA1424214) into the oviduct ampulla using a glass micropipette with tip diameter of ∼10-30 μm ...
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bioRxiv - Molecular Biology 2023Quote: ... and lungs were dissected and placed in ice cold FACS buffer (1% BSA, 3% FBS, 96% Ca2+ and Mg2+ free PBS) with 1 μg/ml Actinomycin D (ThermoFisher BP606-5). After isolation ...
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bioRxiv - Synthetic Biology 2024Quote: ... HEK293FT and HEK293FT-LP cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco 25300-054). All HEK293FT cell lines generated by engineering either the HEK293FT or HEK293FT-LP parent cell lines were cultured in the same way ...
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bioRxiv - Cell Biology 2023Quote: ... were seeded on the top of the membrane and cultured for 3 days in DMEM/F12 (1/1) supplemented with 5 % fetal bovine serum (Fetal Clone II; Hyclone, Thermo Scientific, France), 0.2 ng/ml EGF (Gibco) ...
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bioRxiv - Genomics 2023Quote: ... All nuclei were pooled and stained with DAPI (4′,6-diamidino-2-phenylindole, 3 uM final) (Invitrogen, D1306). Using a FACSAria III cell sorter (BD Biosciences) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were marked with 2,3x10-3 µg/µL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 min at RT in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was assessed at different time points (3, 5, 7 and 9 days) using AlamarBlue Cell Viability Reagent (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were centrifuged at 135 x g for 1 minute and resuspended in 6 ml of hepatocyte media (William’s E media (Gibco, 22551-022) with 3% fetal bovine serum (Gibco ...
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bioRxiv - Genomics 2021Quote: ... 50μg isotope-matched IgG and 50μl protein A/G agarose beads (A:G = 1:1, ThermoFisher 15918014 and ThermoFisher 15920010) and rotated at 4℃ for 8h to preclear the chromatin ...
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bioRxiv - Cell Biology 2022Quote: ... INS-1 cells were transfected in 6-well plates with 5 nM siRNA using Lipofectamine RNAiMAX (Invitrogen, 13778030) and typically analyzed after 96 hours ...
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bioRxiv - Biophysics 2021Quote: ... Desiccated samples were subjected to 5-6 rounds of desiccation and rehydration (1-hour speedvac desiccation [Thermo Fisher Scientific] followed by resuspension in water) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were subcultured at a ratio of 1:10 every 5–6 days using 0.5 mM EDTA (Invitrogen) to detach clumped cells.
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bioRxiv - Evolutionary Biology 2023Quote: ... The transfection reagent was mixed with the pOG44 Flp-Recombinase expression vector and the pCDNA5/TO/FRT-3xMyc-eGFP-CLIP-170 WT or one of the mutant vectors with a 3:1 ratio in Opti-MEMTM I medium (Gibco, ThermoFisher Scientific). Selection started 48h after the transfection in a Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Biochemistry 2023Quote: ... 5’-UUCUCCGAACGUGUCACGUTT-3’ and 5’-ACGUGACACGUUCGGAGAATT-3’ using Lipofectamine RNAiMIX reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... on Step One Plus or ViiA 7 Real-Time PCR System (ThermoFisher Scientific). The primers used for qRT-PCR were designed with Primer Express v3.0 software (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... and 7 μL ddH2O using the ABI Step One (Thermo Fisher Scientific, USA) with two technical replicates ...