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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Enteroids were then washed with PBS three times with the addition of 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (1:5000) (Invitrogen) for the final 10 min wash ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed and stained with 1:10,000 DAPI (4=,6-diamidino-2-phenylindole, Thermo Fisher Scientific; catalog #: D3571) for 15 minutes at room temperature before coverslips were applied using PBS + glycerol as mounting medium.
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with 0.1% Triton X-100 in PBS supplemented with 4′,6-diamidino-2-phenylindole (DAPI) (1 μg/ml) (Invitrogen, # 62248) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... both at 1:300 dilution and mounted with ProLong Gold antifade reagent with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen). Confocal microscopy (Olympus FLUOVIEW FV3000 ...
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bioRxiv - Immunology 2022Quote: ... The cells were centrifuged at 400 g for 5 min at 4°C and resuspended in DPBS (GIBCO) supplemented with 5% FBS ...
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bioRxiv - Microbiology 2019Quote: ... and 3-6 μl Lipofectamin 2000 (Life Technologies). See the Supplemental Information for detailed description.
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bioRxiv - Immunology 2020Quote: ... and QuantStudio 3 or 6 Flex (ThermoFisher Scientific) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
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bioRxiv - Microbiology 2020Quote: ... and 1 mM PMSF and pre-cleared for 2h at 4°C by adding magnetic Protein G Dynabeads (Life Technologies). Beads were discarded and 1 µg of anti-CTCF antibody (Diagenode #C15410210 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific). Pre-cleared supernatants were incubated overnight at 4°C with 1:1250 dilution of Rabbit (DAIE ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was removed after being centrifuged for 30 min at 20,000 × G and 1 mL of cold (4 °C) 80/20 methanol/water (Optima, LC/MS Grade, Fisher Scientific) was added to the remaining contents ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated with anti-IgM antibody (1/100) (Jackson Immuno Research) for 30 min at 4°C and mounted in Fluoromount-G with DAPI (Invitrogen). Fluorescence was detected using an inverted confocal microscope ...
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bioRxiv - Microbiology 2023Quote: ... The viral pellets were then concentrated at 70,000 x g for 1 hour at 4°C and gently resuspended in 140 ul of DPBS (Gibco; 14190144) and allowed to resuspend overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated o/n at with rotation at 4°C with 2 µg IP antibody and for 1 h with Dynabeads Protein G (Invitrogen). Alternatively ...
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bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Cell Biology 2019Quote: ... and 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) (Invitrogen) in water for 1 hour at 60°C ...
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bioRxiv - Biophysics 2022Quote: ... 19 mg EDC (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (Thermo Fisher), 11 mg sulfo-NHS (N-Hydroxysulfosuccinimide ...
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bioRxiv - Cell Biology 2023Quote: ... 1 % dipalmitoyl PI(3)phosphate (PI(3)P diC16) (Life Technologies) and extruded to 400 nm using a polycarbonate filter and a hand extruder (Avanti Polar Lipids ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was performed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Cell Biology 2019Quote: ... The eluate was mixed 1:1 with 8 mg/ml Proteinase K (Thermo Fisher 25530015) in proteinase K buffer for 1 h in a thermomixer at 37° C at 850 RPM ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-ZO-1 (1:100, Cat #14-9776-8; Thermo Fisher Scientific, Waltham, MA, USA), anti-RPE65 (1:100 ...
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bioRxiv - Plant Biology 2023Quote: ... using primers GtEFF1 (5’-CCCTGCAAGCTCTTCCTCTTAG-3’) and GtEFR1 (5’-GCATGCGAGGTCCCAAAA-3’) with the TaqMan probe (5’-6FAM-ACTGCACAGACCATC-MGB-3’) (Thermo Scientific™, USA) (Keenan et al. ...
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bioRxiv - Microbiology 2022Quote: ... one volume of 4% Agarose Gel (Gibco, Life Technologies) was mixed by a three-volume of preheated IAV growth media to make the overlay gel liquid and kept at 37 ℃ water bath ...
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bioRxiv - Microbiology 2022Quote: ... one volume of 4% Agarose Gel (Gibco, Life Technologies) was mixed by a three-volume of preheated IAV growth media to make the overlay gel liquid and kept at 37 ℃ water bath ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
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bioRxiv - Neuroscience 2021Quote: ... and immersed in reagent-2 (diluted 1:2 in PBS) for 6-24 h before incubated in reagent-2 containing TO-PRO-3 (1:5,000, Thermo Fisher Scientific) for additional 7-10 days ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 g of agarose powder (Fisher Scientific, UK), and 1.5 μl of SYBR™ Safe DNA Gel Stain (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... the sample was washed 3 x 5 min with PBS and mounted in Fluoromount-G (ThermoFisher, 00-4958-02). 15 samples per genotype were prepared and photographed using a tile scan at a confocal microscope Zeiss LSM780 (Zeiss ...
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bioRxiv - Microbiology 2020Quote: ... Fungal hyphae were stained with 0.5 mM 4.4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (C1-BODIPY 500/510 C12, Thermo Fisher Scientific) or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16 ...
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bioRxiv - Cell Biology 2021Quote: ... sense 5’-CAAAGGACAACUGUCAGACACAGAA-3’ and antisense 5’-UUCUGUGUCUGACAGUUGUCCUUUG-3’) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... sense 5’-CUACAAAGCUGAUGAAGAC-3’ and antisense 5’-GUCUUCAUCAGCUUUGUAG-3’) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Each well was washed with PBS and then incubated for one hour at 4°C while rocking with 1 mg/mL EZ-Link NHS-SS-Biotin (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... LDHA proteins in tumor extract were separated on 4% to 20% Tris-Glycine gel and CD31 was separated on NuPAGE 3 to 8 % Tris-Acetate gel (Life Technologies) by SDS-PAGE and were transferred to nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated at 95°C for 5 min and the supernatant loaded onto a either 3-8% Tris-acetate or 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight 4°C incubation with rabbit polyclonal antibodies against claudin-5 (1:100; Life Technologies) and ZO-1 (1:200 ...
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bioRxiv - Genomics 2022Quote: ... 4 μl of 1 M CaCl2 and 5 μl of 100 U MNase (88216, Thermo Fisher Scientific) were added ...
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bioRxiv - Biochemistry 2019Quote: ... cells were cultured for 48h in the absence and for 3 weeks in the presence of 1 mg/mL G-418 selection antibiotic (Thermo Fisher). Stable cells expressing GFP-fusion proteins were trypsinized and single GFP+cells with scatter properties (FSC and SSC ...
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bioRxiv - Immunology 2019Quote: ... 15 μg of pMul1-FLAG and 15 μg of pAsc-Myc mixed with FuGENE HD at a ratio of 1:3 in 500 μl Opti-MEM (Thermo Fisher) were transfected ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (0.5 μg/cm2) and Lipofectamine 2000 (1 μg RNA:3 μL reagent) were added separately to 50 μL opti-MEM (Thermo Fisher) and incubated for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... PDCA-1 (ThermoFisher # 53-3179-4), TLR9 (Biolegend #394803) ...
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bioRxiv - Pathology 2023Quote: ... Fluo-4-AM (1 µM; Invitrogen), in the dark for 20 minutes at room temperature in Mg2+-PSS ...
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bioRxiv - Neuroscience 2023Quote: ... (1:300; 4 h, RT, Invitrogen) were used as secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... 8 μl of 5% hydroxylamine (Thermo Fisher Scientific) was added to quench the reaction ...
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bioRxiv - Microbiology 2023Quote: ... followed by flushing the solution with 20 μL of DPBS (KCl 0.2 g/L, KH2PO4 0.2 g/L, NaCl 8 g, Na2HPO4 1.15 g/L, pH = 7.0–7.3, Thermo Fisher Scientific, Waltham, MA, United States) (Figure S2A Step 3) ...
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bioRxiv - Bioengineering 2020Quote: ... Scaffolds were washed in PBS at every timepoint (day 1, 4, 7, 14, 28) and placed in a solution of alamarBlue® (Invitrogen, California, USA) in an incubator at 37°C on a shaker ...
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bioRxiv - Cancer Biology 2020Quote: 6 mg of Protein G Dynabeads (Invitrogen, catalog no. 1004D) were washed 3 times then resuspended in PBST (pH 7.2) ...