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Citations for Thermo Fisher :
6401 - 6450 of 10000+ citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... cells were counted (Countess 3 cell counter, Invitrogen, AMQAF2000) and resuspended for seeding or re-engraftment in suitable media ...
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bioRxiv - Molecular Biology 2023Quote: ... NuPAGE™ 3 to 8% Tris-Acetate gels (Invitrogen) were used.
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bioRxiv - Cancer Biology 2023Quote: ... AML-3 were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse TNFα ELISA Kit (Thermo Fisher Scientific, BMS607-3), Mouse IL6 ELISA Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific), QuantStudio 5 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... acidified to pH2-3 by trifluoroacetic acid (Thermofisher, 85183), and desalted with a Pierce C18 spin column (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... counted on a Countess 3 automated cell counter (Invitrogen) and reseeded at 200 000 cells or 80 000 cells per well when kept for two or three days respectively.
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bioRxiv - Molecular Biology 2023Quote: ... cell numbers were determined by Countless 3 (Thermo Fisher). Cell pellet was resuspended in 300-600 μL acidic extraction buffer (0.3 M HCl ...
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bioRxiv - Physiology 2024Quote: ... and RT- qPCR performed by QuantStudio 3 (Applied Biosystems) with SYBR Green PCR Master Mix (Applied Biosystems 4309155) ...
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bioRxiv - Developmental Biology 2024Quote: ... 3 kDa Texas-red Dextran (Thermo Fisher, 500 μM) was injected into the periotic space of embryos at 48 hpf ...
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bioRxiv - Developmental Biology 2024Quote: ... To stain nuclei we used To-Pro 3 (Invitrogen), 1:1000.
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bioRxiv - Cell Biology 2024Quote: ... The caspase 3/7 substrate activity assay (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... mouse-anti-desmocollin-2/3 7G6 (1:250, Invitrogen 32-6200 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cleaved Caspase-3 (Asp175) (1:200, Invitrogen PA5-114687), Tom20 (1:400 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3 µL of 10mM dNTP (Fisher Scientific #PR-U1515), 1.5 µL of Q5 polymerase (NEB #M0491L) ...
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bioRxiv - Biochemistry 2024Quote: ... membranes and blocked with 3% I-block (ThermoFisher, T2015) for at least 1 hour ...
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bioRxiv - Genetics 2024Quote: ... 3 U Super-Therm Taq DNA polymerase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Zn2+ levels were measured using FluoZin-3 (Invitrogen, #F24194) as described previously 16 ...
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bioRxiv - Bioengineering 2024Quote: ... CellEventTM Caspase-3/7 green detection reagent (Invitrogen, C10423) by 1:400 to achieve 5 μM in complete growth medium ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 U Platinum High Fidelity Taq DNA polymerase (Invitrogen), and 4 μL sample DNA and distilled H2O to reach a final volume of 50 μL ...
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bioRxiv - Physiology 2024Quote: ... with a QuantStudio 3 PCR System (Thermo Fisher Scientific). Target gene threshold cycle (Ct ...
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bioRxiv - Physiology 2024Quote: ... on the Quant Studio 3 PCR system (Applied Biosystems). The expression levels of AChE (Forward ...
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bioRxiv - Neuroscience 2024Quote: ... with To-Pro-3 0.5 µM (Thermo Fisher, T3605). SLO (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed on a QuantStudio 3 (Applied Biosystems) thermocycler using TaqMan reactions for Cx43 (GJA1 ...
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bioRxiv - Bioengineering 2024Quote: ... RT-PCR was performed via QuantStudio 3 (Applied Biosystems) in 20 µL reactions containing 10 µL of Power Track™ SYBR Green Master Mix (Thermo Fisher Cat#A46012) ...
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bioRxiv - Neuroscience 2024Quote: ... Blasticidin (3 μg/ml, Thermo Fisher Scientific, MA, USA) and Zeocin (125 μg/ml ...
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bioRxiv - Physiology 2024Quote: ... and for 3 min with DAPI (Invitrogen, Carlsbad, USA). Slides were mounted with a drop of mounting medium (Fluoroshield ...
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bioRxiv - Cell Biology 2019Quote: ... Caspase-3/7 activation in live cells was monitored using CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific, C10423, 1:1000) in IncuCyte Live Cell Analysis System ...
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bioRxiv - Immunology 2021Quote: ... Mice were genotyped by PCR using forward primers 5’-ctgagcagagacccactgaaag-3’ and reverse primers 5’- ggatctggcttctgagtttgtgta-3’ and amplicons were ran in 6% TBE gels (Life Technologies, Carlsbad, CA).
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bioRxiv - Molecular Biology 2021Quote: ... The probe was biotin labeled at the 3’ end using a Pierce™ Biotin 3’ End DNA Labeling Kit (Thermo Fisher Scientific Inc.). Seven micrograms of nuclear protein were added to a binding reaction mixture containing 2µl 10X binding buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... oligonucleotide duplexes were designed against TG2 (sense, 5’-AAGGGCGAACCACCTGAACAA-3’ and antisense, 5’-TTGTTCAGGTGGTTCGCCCTT-3’) and TOPOIIα siRNA (purchased from Thermo Fisher, Catalog # AM16708). Plasmids and miRNA were transfected with Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Bioengineering 2019Quote: ... via covalent attachment to COOH groups on the particles via standard EDC chemistry using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Thermo Fisher Scientific, MA) and N-hydroxysulfosuccinimide sodium salt (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... We did this by amplification of the GlyR-FP plasmids using PCR with primers 5’-ATATGGTACCTGGGAGGTCTATATAAGCAGAG-3’ and 5’ATAAGGTACCCCAGGCGGGCCATTTACCGTA-3’ followed by digestion with KpnI (ThermoFisher Scientific, Merelbeke, Belgium) and ligation using instant sticky-end ligase Master mix (NEB ...
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bioRxiv - Microbiology 2020Quote: Vero E6 and Calu-3 cells (Calu-3:ATCC HTB-55; Vero E6: ATCC, CRL-1586) were maintained in high glucose DMEM (Gibco, Waltham, MA, USA) supplemented with 10% FBS (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis was measured by caspase-3/7 staining using CellEvent® Caspase-3/7 Green ReadyProbes® Reagent (ThermoFisher Scientific Inc., cat# R37111). CHLA20 and NGP cells were grown until confluence on 6-well plates with six days of treatment with DMSO or GSK591 ...
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bioRxiv - Cell Biology 2020Quote: ... 16nM TIMM23 (5’ CCCUCUGUCUCCUUAUUUA 3’, Eurogentech) or 16nM TIM22 (5’ GUGAGGAGCAGAAGAUGAU 3’, Eurogentech) using the Invitrogen Lipofectamine RNAiMAX Reagent (Invitrogen, Carlsbad, CA, USA) diluted with serum-free Gibco Opti-MEM I medium (Gibco ...
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... cells were transfected with siRNAs targeting human SNX13 (5’- CAGAAAGGCUCAACAGAAAUU-3’) or SNX14 (5’-GGAUGAAAGUAUUGACAAAUU-3’) using Lipofectamine RNAiMax (Invitrogen, Cat#13778-075) according to the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 3 kb RT-PCR products covering the S gene deletion were amplified from the viral RNA using the gene specific primers F9newF and F9newR (5’-TAAGGTTGGTGGTAATTATAATTACCTG-3’ and 5’-AAAATAGTTGGCATCATAAAGTAATGGG-3’) and a SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThemoFisher). A region spanning the deletion was sequenced using primers Wu_24_L and Wu_24_R (5’-TTGAACTTCTACATGCACCAGC-3’ and 5’-CCAGAAGTGATTGTACCCGC-3’).
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bioRxiv - Molecular Biology 2020Quote: ... 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP, dGTP and dCTP, 3 mM dTTP and 2 mM 5-(3-aminoallyl)-dUTP (Thermo Fisher Scientific, AM8439)) and 1.25 µL of 40 µM oligo 1 ...
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bioRxiv - Microbiology 2021Quote: ... A recombinant lentivirus vector expressing the coding sequence of the EBV transactivator BZLF1 under control of a tetracycline-regulated promoter was constructed by cloning the open reading frame amplified with the primers 5’-CGACCGGTATGATGGACCCAAACTCGAC-3’ and 5’-CGACGCGTTTAGAAATTTAA GAGATCCTCGTGT-3’ into the Age I and Mlu I sites of the pTRIPZ lentiviral vector (Thermo Fisher Scientific, USA). For virus production ...
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bioRxiv - Neuroscience 2022Quote: ... 10% 3 kD or 10 kD biotinylated dextran amines (3 kD BDA, Invitrogen D7135; 10 kD BDA Invitrogen D1956; diluted in 0.9% NaCl) were used as retrograde and anterograde tracers ...
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bioRxiv - Molecular Biology 2022Quote: ... pDNOR207-ZNF451-1 (isoform 1) and pDNOR207-ZNF451-3 (isoform 3) were generated using the Gateway® cloning BP reaction (Thermo Fisher Scientific) upon cDNA amplification using BP-tailed primers and pDNOR207 as donor vector ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were washed in 3 times in 3% PBST (10 min) and incubated in goat anti-chicken Alexa Fluor 488 (Invitrogen, A11039, 1:1000) in blocking buffer at room temperature for 2hrs ...
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bioRxiv - Evolutionary Biology 2021Quote: ... amplified with a set of forward (5’-GAGGGTGAGCTCTCCGAAGGTTGTAG-3’) and reverse (5’-AATTATGAGCTCTGGGAG-TGCGCAAG-3’) primers using DreamTaq DNA Polymerase (Thermo Fisher Scientific, USA). The isolated genomic DNAs were also subjected to amplify full length betasatellites using forward (5’-AGTAAGGGTACCACTACGCTACGCAG-3’ ...
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bioRxiv - Immunology 2020Quote: ... qPCR for parasite burden was conducted using toxoplasma specific primers: (forward) 5’-TCCCCTCTGCTGGCGAAAAGT-3’ and (reverse) 5’-AGCGTTCGTGGTCAACTATCGATT G-3’ and Power SYBR Green master mix (Applied Biosystems, CA, USA). The qPCR condition settings were ...
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bioRxiv - Microbiology 2022Quote: ... pTG-Luc and SARS2-COV2 spike expression vector were transfected into HEK 293T cells at the ratio of 3:4:3 using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific, Waltham, MA). Accession IDs of the spike proteins used in the study were ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated in 2 μM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, cat no: T3168) at 37°C for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was replaced every 2-3 days and cells passaged 1:2 or 1:3 weekly with 0.25% Trypsin/EDTA (Thermo Fisher Scientific, #25200-056) pre-warmed at 37°C.
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bioRxiv - Cancer Biology 2024Quote: ... 48hrs after transfection the cells were fixed and stained for activated Caspase-3/7 using Apoptosis CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) according to manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16°C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3).