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Citations for Thermo Fisher :
6351 - 6400 of 10000+ citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... the cells in each well were transfected with 20 pmol siRNA targeting to human XPO1 (GenePharma, Shanghai, China) using lipofectamine 2000 (Invitrogen). At 6 h after transfection ...
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bioRxiv - Molecular Biology 2020Quote: Efficiency of all qPCR probes was tested by using a simplex 2-fold serial dilution of Quantigene commercial reference human RNA (Invitrogen) or DNA (Promega ...
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bioRxiv - Immunology 2020Quote: ... and SARS-CoV-2 and the N-terminal peptidase domain of human ACE2 (residues Ser19-Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously described 18,19,37-39 ...
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bioRxiv - Biochemistry 2020Quote: In vitro 3D epidermal skin equivalents were generated as previously described.[28] Human neonatal keratinocytes (HEKn, ThermoFisher Scientific, Loughborough, UK) were maintained as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: We purified polyclonal IgG from human plasma samples using Pierce™ Protein G Ultra Link™ Resin (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Fibrin formation was visualized by spiking whole blood with human fibrinogen conjugated to Alexa Fluor 647 (Cat. No. F35200, Invitrogen, used at final concentration of 7.5 μg/mL) ...
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bioRxiv - Cancer Biology 2021Quote: ... PLK2 in a pDONR223 vector was obtained from human ORFeome (Open Biosystems-Horizon Discovery) and was cloned into pCW57.1 using the Gateway recombination reaction (Life Technologies) and verified by sequencing prior to transfection ...
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bioRxiv - Neuroscience 2020Quote: ... and the SH-SY5Y human neuroblastoma cell line was maintained in 10% FBS Minimum Essential/F12 medium (MEM/F12, Gibco). Both cells’ media included 1% antibiotic/antimycotic mix (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ADAP1CRISPR and CtrlCRISPR TM were left resting or stimulated with Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell ...
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bioRxiv - Microbiology 2021Quote: ... and the N-terminal peptidase domain of human ACE2 (residues Ser19–Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as described previously21 ...
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bioRxiv - Immunology 2020Quote: ... Acquired DDA .raw files were searched against the human UniProtKB/SwissProt database (v2012_07) using Byonic (Protein Metrics) embedded in Proteome Discoverer (ThermoFisher Scientific) to obtain peptide sequence information ...
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bioRxiv - Microbiology 2021Quote: Individual plasmids encoding 11 human genes or empty control vector were transfected individually into U-87 MG cells with Lipofectamine 3000 (Invitrogen) 48 hours prior to infection with VEEV-TC-83-nLuc.
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bioRxiv - Microbiology 2021Quote: Huh-7 cells seeded in 6 well plates were transfected with human WWOX-specific or random siRNAs (DHARMACON) at a final concentration of 50nM per well using Lipofectamine 2000 reagent (INVITROGEN). At 24 hours post siRNA transfection ...
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bioRxiv - Microbiology 2022Quote: ... gondii parasites of the type I RH strain Δku80/Δhxgprt genetic background (ATCC PRA-319) were grown in human foreskin fibroblasts (HFFs, ATCC SCRC-1041) maintained in DMEM (GIBCO) supplemented with 3% inactivated fetal calf serum (IFS ...
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bioRxiv - Genetics 2022Quote: ... Genotyping was done using Axiom™ Human Genotyping SARS-CoV-2 Research Array as instructed by the manufacturer (Thermo Scientific).
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bioRxiv - Immunology 2022Quote: A recombinant fragment of human SP-D (rfhSP-D) was expressed under bacteriophage T7 promoter in Escherichia coli BL21 (λDE3) pLysS (Invitrogen), transformed with for plasmid containing cDNA sequences for neck ...
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bioRxiv - Immunology 2022Quote: Human bronchial epithelial cells (BEAS-2B cells, ATCC® CRL-9609™) were cultured in LHC-9 medium (Life Technologies) and human lung fibroblast (MRC-5 cells ...
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bioRxiv - Immunology 2022Quote: ... were generously provided by Anita Koshy (University of Arizona) and maintained by serial passage through human foreskin fibroblast (HFF) monolayers in parasite culture medium (DMEM [Gibco] ...
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bioRxiv - Immunology 2022Quote: ... and maintained by serial passage through human foreskin fibroblast (HFF) monolayers in parasite culture medium (DMEM [Gibco], 20% Medium 199 [Gibco], 10% FBS [Gibco] ...
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bioRxiv - Immunology 2022Quote: ... and maintained by serial passage through human foreskin fibroblast (HFF) monolayers in parasite culture medium (DMEM [Gibco], 20% Medium 199 [Gibco] ...
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bioRxiv - Genetics 2022Quote: ... hiPSC-CMs were then transfected with siRNAs directed against PAX9 and EGR2 (ON-TARGETplus Human) using lipofectamine RNAi Max (ThermoFisher). Each siRNA was tested individually in 8-plicates ...
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bioRxiv - Cell Biology 2022Quote: Toxoplasma gondii RHΔhxgprt strain was maintained by growth in confluent primary human foreskin fibroblasts (HFFs) in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... Sorted Tregs were pre-activated for 48h using α-CD3/CD28 coated beads (Dynabeads Human T-Activator, Thermo Fisher scientific) in a 1:1 ratio (beads to cells) ...
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bioRxiv - Microbiology 2022Quote: Murine macrophage-like RAW 264.7 cells and human A549 lung epithelial carcinoma cells were cultivated in RPMI 1640 medium (Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2019Quote: BJ-5ta hTERT-immortalized human fibroblasts were licensed from ATCC (CRL-4001) and cultivated in Dulbecco’s Modified Eagles Medium Glutamax® (Thermofisher) with 20% Medium 199 (Earles Salts ...
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bioRxiv - Microbiology 2019Quote: ... and HEK293T (embryonic human kidney epithelial, ATCC®CRL-3216) cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2019Quote: ... The immortalized human upper airway epithelial cell line A549 (ATCC# CCL-185) was maintained in minimal essential medium (MEM; Gibco) supplemented with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2019Quote: ... were incubated for 15 minutes at 37°C in the presence of Alexa-fluor 555 labelled human transferrin (Thermo Fisher) (25 μg/ml ...
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bioRxiv - Microbiology 2019Quote: ... the membrane was probed with HIV-Ig as the primary antibody and corresponding secondary antibody conjugated to horseradish peroxidase (goat anti-human IgG, Invitrogen). Gag-specific bands were detected after incubation with SuperSignal West Pico chemiluminescent substrate (Pierce ...
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bioRxiv - Cancer Biology 2019Quote: ... Precursors of respective human microRNAs (miR-27a, −122, −128, −193 and −217) and negative controls were purchased from Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... hiPSCs were cultured on plates pre-coated with 0.5 μg/cm2 recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) with daily changes of antibiotic-free Essential 8 (E8) media (ThermoFisher). Cells were passaged as small clumps with Versene (ThermoFisher) ...
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bioRxiv - Immunology 2019Quote: ... Samples were analyzed on the GeneChip Human Gene 2.0 ST Array chip hybridized on the GeneChip Scanner 3000 7G System (Applied Biosystems) according to the manufacturer’s recommended instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Medium from each condition was removed and E-cadherin concentrations were quantified by using Human E-Cadherin Quantikine ELISA kit (Invitrogen). The Human E-Cadherin EIA kit protocol was followed as described ...
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bioRxiv - Immunology 2019Quote: ... Concentrations of 30 cytokines were measured using the 30-plex magnetic human Luminex cytokine kit (all using lot ID 1805187A, ThermoFisher). Samples were measured on a LX200 (Luminex ...
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bioRxiv - Bioengineering 2020Quote: ... falciparum parasite culture was maintained in 5% human red blood cells purified from whole blood (Research Blood Components) with complete RPMI medium (Gibco) supplemented with hypoxanthine (Sigma-Aldrich) ...
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bioRxiv - Genomics 2019Quote: Hand-picked human pancreatic islets were dissociated to single cells using enzyme-free Hanks’-based Cell Dissociation Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... and treated without (vehicle) or with Gibco™ Dynabeads™ Human T-Activator CD3/CD28 (Cat. No. 111.61D, ThermoFisher Scientific) in the presence of recombinant IL2 and BD GolgiPlug (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... WT human PRRT2 (gene ID: 112476) was synthesized as N-terminal HA tag fusion protein (Genewiz) in pcDNA3 vector (Thermofisher) and single point mutants were generated on this matrix by standard molecular biology techniques.
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bioRxiv - Neuroscience 2021Quote: ... tau proteins were measured with Tau-5 antibodies using ELISA according to manufacturer’s instructions (The Human Tau Solid-phase sandwich ELISA, #KHB0041, Thermo Scientific).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... as well as human hepatoma Huh7.5 cells28 were maintained at 37 °C with 5% CO2 in Dulbecco’s Modified Medium (DMEM) (Invitrogen, Paisley, UK) containing 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Parasites were maintained by continuous passage in human foreskin fibroblasts (HFFs) in Dulbecco’s Modified Eagle’s Media (DMEM) (ThermoFisher, Carlsbad CA) containing 1% (v/v ...
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bioRxiv - Genomics 2020Quote: Female human bone osteosarcoma U-2 OS cells (ATCC HTB-96) were routinely cultured in McCoy’s 5A (Modified) medium (ThermoFisher 16600) with 10% FBS (Gemini Bio ...
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bioRxiv - Cancer Biology 2020Quote: ... isolation media was changed to human liver expansion media (EM; Ad+++ supplemented of 1X B27 supplement without retinoic acid (Gibco), 1X N2 supplement (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells and human mesenchymal stem cells were grown in DMEM culture medium supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 mg/ml streptomycin and 100 units/ml penicillin (Gibco ...
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bioRxiv - Immunology 2020Quote: ... and resuspended in complete medium in the absence of human serum or Pf-iRBCs schizonts were isolated in RPMI 1640 medium supplemented with 0.25% Albumax (GIBCO, Invitrogen) and 7.4% Sodium Bicarbonate (GIBCO ...
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bioRxiv - Immunology 2020Quote: ... Mass spectrometry derived data was searched against Human RefSeq 81 protein database in Proteome Discoverer 2.1 (Thermo Scientific, Bremen, Germany) using SEQUEST and Mascot (version 2.5.1 ...
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bioRxiv - Molecular Biology 2021Quote: Human proximal renal tubular epithelial cells (HK2) were purchased from ATCC (ATCC®CRL-2190) and cultured in DMEM (Gibco) supplemented with 10% FBS (Moregate) ...
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 (Apath) cells were maintained in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen, 10-013-CV); African green monkey kidney Vero (ATCC ...
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bioRxiv - Microbiology 2021Quote: ... and the N-terminal peptidase domain of human or mouse ACE2 (residues Ser19–Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as described previously44 ...