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Citations for Thermo Fisher :
6201 - 6250 of 10000+ citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CFSE-stained T cells were resuspended at 1e6/ml and DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) were added at a ratio of 1:10 beads:cells ...
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bioRxiv - Biochemistry 2024Quote: The full-length wild-type human NTCP gene (UniProt ID: Q14973) was generated synthetically by GeneArt (Thermo Fisher Scientific) after codon optimization for expression in HEK293 cells ...
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bioRxiv - Neuroscience 2023Quote: Human dermal fibroblasts were obtained from skin biopsies and cultivated in Dulbecco’s modified eagle medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... ∼100,000 human bladder epithelial cells (RRID: CVCL_0126) were seeded in 24-well plates overnight in RPMI (Gibco; ThermoFisher Scientific) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Microbiology 2024Quote: ... ∼100,000 human bladder epithelial cells (RRID: CVCL_0126) were seeded in 24-well plates overnight in RPMI (Gibco; ThermoFisher Scientific) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Immunology 2024Quote: IFNγ in supernatants from the whole blood RSA was determined by ELISA according to the manufacturer’s instructions (Human IFNγ Uncoated ELISA kit; Invitrogen). 50μl of supernatant was diluted with 50μl of assay diluent for use in the IFNγ ELISA ...
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bioRxiv - Immunology 2024Quote: Primary human PBMCs and CAR T derivatives thereof were cultured in RPMI 1640 (Gibco, Life Technologies, Carlsbad, CA, USA) with 10% FCS ...
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bioRxiv - Immunology 2024Quote: Primary human PBMCs and CAR T derivatives thereof were cultured in RPMI 1640 (Gibco, Life Technologies, Carlsbad, CA, USA) with 10% FCS ...
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bioRxiv - Physiology 2024Quote: ... The PCR amplification of human Piezo1 was performed using a Veriti Thermal Cycler (Thermo Fisher Scientific, Waltham, MA, USA) and ExTaq (Takara ...
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bioRxiv - Evolutionary Biology 2024Quote: ... fragments of human L1 ORF1 and ORF2 from the EF06R plasmid were amplified by PCR and cloned into pcDNA3.1(+) (#V79020, Invitrogen) with the puromycin resistant gene split by an intron ...
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bioRxiv - Microbiology 2024Quote: ... and human embryonic kidney-293T (HEK-293T) cells (ATCC CRL-3216) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco), with 10% heat-inactivated FBS (Millipore Sigma) ...
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bioRxiv - Genomics 2024Quote: HEK 293T and human foreskin fibroblasts (HFF) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific), supplemented with 10% (vol/vol ...
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bioRxiv - Genomics 2024Quote: ... human female) cells were cultured in a 37°C 5% CO2 and 20% O2 humidified incubator with DMEM (Gibco) supplemented with 10% heat-inactivated FBS (Gibco) ...
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Interaction between gene expression and morphokinetic parameters in undisturbed human embryo culturebioRxiv - Developmental Biology 2024Quote: ... Labelled PolyAcDNA was then hybridised to the GeneChip® Human Genome U133 Plus 2.0 Array (Affymetrix, SantaClara, CA, USA) that covers over 47,000 transcripts and have been commonly used for human transcriptome analysis ...
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bioRxiv - Developmental Biology 2024Quote: Human Umbilical Vein Endothelial Cells (HUVEC) (ATCC, Cat. #: PCS100010) were culture in 1X M-199 Medium (Gibco, Cat. #: 12340030) supplemented with 16 % FBS (Gibco ...
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bioRxiv - Immunology 2024Quote: ... The following fluorochrome-labeled anti-human Abs were used for surface staining: PerCP/Cy5.5-CD19 (Invitrogen, #45-0198-42), APC-CD27 (eBioscience ...
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bioRxiv - Cell Biology 2024Quote: ... Human precision cut lung slices (PCLS) were purchased from IIVS and were grown in DMEM/F-12 media (Gibco) supplemented with antibiotics and antimycotic reagents.
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bioRxiv - Neuroscience 2024Quote: ... Human iPSCs were cultured as colonies in feeder-free conditions using Essential 8 Flex Medium (Thermo Fisher Scientific, A2858501) on Matrigel hES-qualified (Corning ...
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bioRxiv - Biochemistry 2024Quote: Protein in mouse and human wound fluid samples were quantified with the BCA Protein Assay kit (Thermo Fisher Pierce) using the 96-well plate protocol recommended by the manufacturer ...
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bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: RNA extracted from human left ventricular tissue was digested with DNase I (Invitrogen DNA-free DNA removal kit AM1906). 20 ng digested RNA was depleted of rRNA (NEBNext rRNA depletion kit E6350L) ...
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bioRxiv - Microbiology 2024Quote: Promutagens were first activated by incubating in equal volumes with a 2-fold human microsomal (ThermoFisher Cat. No. HMMCPL) reaction mixture containing 1mg/mL microsomes ...
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bioRxiv - Molecular Biology 2024Quote: Quantitation of human DNA isolated from blood cells was performed using Qubit 2.0 with the dsDNA High Sensitivity Assay Kit (Thermofisher A custom Ampliseq panel for the detection of SNPs was designed using Ion AmpliSeq Designer and ordered as Ampliseq Custom Panel ...
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bioRxiv - Microbiology 2024Quote: The TR146 human buccal epithelial squamous cell carcinoma cells (MilliporeSigma) 50 were incubated in DMEM/F12 medium (Gibco, #11320033) with 10% fetal bovine serum and 1x penicillin-streptomycin (Gibco ...
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bioRxiv - Microbiology 2024Quote: T24 human epithelial bladder cells (ATCC HTB-4) were grown in McCoy’s 5A modified medium (Gibco 16-600-082) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... activated with anti-CD3/anti-CD28 activation beads per manufacturer’s instructions (Gibco Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation, Thermofisher), stimulated with IL-2 (R&D) ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated from human samples using Ficoll-Paque density centrifugation (Corning, Fisher Scientific, CA). PBMC were cultured in RPMI 1640 medium (Corning ...
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bioRxiv - Immunology 2024Quote: ... and Spike protein expressing pcDNA3.1-SARS-CoV-2 SΔCT were co-transfected into human embryonic kidney (HEK)293 T cells (ATCC CRL_3216) with lipofectamine 2000 (ThermoFisher Scientific). Pseudo-typed viruses of SARS-CoV-2 variants were generated using the Spike protein from WA1/2020 (Wuhan/WIV04/2019 ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... a secondary Alexa Fluor 647 conjugated goat anti-human IgG (H+L) antibody (Invitrogen #A-21445; 10 μg/mL) was used.
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bioRxiv - Molecular Biology 2024Quote: ... All human L1 plasmids contain the indicated fragments of L1.3 DNA (a retrotransposition-competent human L1, GenBank accession no. L19088 (32)) cloned into pCEP4 (Invitrogen) unless indicated otherwise ...
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bioRxiv - Neuroscience 2022Quote: ... Culture 1 1% (Gibco), fetal bovine serum 2.5% and 2 μg/ml doxycycline hydrochloride.
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bioRxiv - Cancer Biology 2022Quote: ... mixed 1:1 with addition of 1% B27 (Invitrogen), 0.5% N2 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... treated 1:1 with chloroform (Fisher Scientific, C606-1) for 15 minutes ...
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bioRxiv - Biophysics 2020Quote: ... The concentrations of cdM2-1 and RNA were determined by absorbance at 280 and 260 nm using extinction coefficients of 5,960 M−1·cm−1 (Expasy-ProtParam) (14) and 8,050 M−1·cm−1·base−1 (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... and re-suspended at a density of 104 cells/mL in pre-warmed complete minimum essential media (MEM) that contains 5 mg/mL human recombinant insulin (Gibco), 1X MEM non-essential amino acids (NEAA ...
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bioRxiv - Biophysics 2021Quote: A stable β2AR-overexpressing cell line was produced by transfecting CHO-K1 cells (ECACC) with a plasmid coding for the human β2AR with C-terminal GFP using lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The concentrations of IgG in cell lysates and supernatants after recycling were measured with an IgG Human ELISA Kit (88-50550, Invitrogen), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... the culture medium was switched to standard human embryonic stem cell (hESC) medium containing DMEM/F12 (Thermo Fisher Scientific, 11320033), KnockOut Serum Replacement (20% ...
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bioRxiv - Cell Biology 2020Quote: ... Each labeled sample was assayed with the Affymetrix PrimeView human gene expression array read with an Affymetrix GeneChip Scanner 3000 (Affymetrix/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... cells were subsequently pulsed at 370C for different time points with 25µg/ml Alexa flour-488 or- 568-conjugated human transferrin (Invitrogen, ThermoFisher Scientific) reconstituted in starvation medium and clarified by centrifugation at 15,000 × gmax at 40C for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Bovine or human TLR2 sequences of interest were cloned into pcDNA3.3 TOPO TA mammalian expression vector (Thermo Fisher Scientific, UK), and transfected into SEAP HEK cells using TurboFect transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... The extraction and purification of human genomic DNA were performed using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific) and then the quantification was conducted using the Quantifiler Human DNA Quantification Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... cerevisiae EKY3/pYES2-TOP1 induced for expression of wild-type human TOP1with the PrepEase Histidine-Tagged Protein Purification Midi Kit – High Specificity (from Affymetrix) according to the manufacturer’s procedures ...
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bioRxiv - Immunology 2020Quote: Frozen human peripheral blood mononuclear cells (PBMCs) from blood donors were thawed and diluted into cold RPMI 1640 medium (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2020Quote: ... and the secondary antibodies including horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (#31439) and HRP-conjugated goat anti-human IgG (#31410) were purchased from Invitrogen. The secondary antibody for HRP-conjugated goat Anti-rabbit IgG (#12-348 ...
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bioRxiv - Genomics 2020Quote: Primary human keratinocytes were isolated from fresh surgically discarded neonatal foreskin and cultured in Keratinocyte-SFM (Life Technologies 17005-142) and Medium 154 (Life Technologies M-154-500) ...
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bioRxiv - Immunology 2022Quote: ... we labeled the gp120-specific antibody (A32 mAb) with Allophycocyanin (APC) following the manufacturer instructions (Zenon Human IgG labeling kit, Invitrogen) and measured cell binding by flow cytometry ...
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bioRxiv - Physiology 2022Quote: ... human TACAN or their mutants were synthesized by in vitro transcription using the T7 mMESSAGE mMACHINE kit (Invitrogen, Waltham, MA) and injected into oocytes (25 ng each) ...
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bioRxiv - Systems Biology 2022Quote: ... RNA from human skin and wounds was reverse transcribed using TaqMan® Advanced miRNA cDNA Synthesis Kit (Thermo Fisher Scientific). Individual miRNA expression was then quantified using TaqMan® Advanced miRNA Assays (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Ambion™ Silencer™ Pre-Designed siRNA specific of human GLUT3 (SLC2A3) was purchased from Fisher Scientific (#10446914, Illkirch, France)