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Citations for Thermo Fisher :
6201 - 6250 of 7736 citations for Recombinant Human SRPK1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: CAFs were isolated from human colon carcinoma resection specimens following 2.4U/ml dispase + 100U/ml collagenase type II (both from Invitrogen); disaggregation of tumour fragments and digestion for 2h t 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and hybridized to the Affymetrix Human Gene 1.0 ST Arrays using the Gene Chip WT Terminal Labeling and Hybridization Kit (Affymetrix). GeneChip fluidics station 450 was used for processing of the arrays and fluorescent signals were detected with the GeneChip scanner 3000-7 G ...
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bioRxiv - Bioengineering 2021Quote: ... media was changed to the following formulation of EC medium: human endothelial serum-free medium (hESFM, Thermo Fisher 1111044) supplemented with 1% platelet-poor plasma-derived serum (PDS ...
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bioRxiv - Immunology 2020Quote: Human PBMCs were incubated with MitoSOX Red (5μM) and Mitotracker Green (1 μM) (both from Molecular Probes, Life Technologies) in PBS during 10 minutes at 37°C before flow cytometry extra-cellular staining.
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bioRxiv - Immunology 2020Quote: Human PBMCs were incubated with MitoSOX Red (5μM) and Mitotracker Green (1 μM) (both from Molecular Probes, Life Technologies) in PBS during 10 minutes at 37°C before flow cytometry extra-cellular staining.
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bioRxiv - Immunology 2021Quote: ... T lymphocytes were purified using the Pan T cell isolation kit (Miltenyi Biotech) and activated with Dynabeads Human T-Activator CD3/CD28 (1:1 beads:cell) (ThermoFisher) in X-vivo 15 medium (Lonza ...
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bioRxiv - Immunology 2020Quote: ... Primary human CD8+ T cells were cultured for up to 24 days in ATCC-modified RPMI (Thermo Fisher, #A1049101) supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... The human myelomonocytic leukemia cell line THP-1 was propagated in RPMI 1640 medium (GIBCO® Life Technologies™) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Genomics 2019Quote: ... Activation was performed by addition of Dynabeads Human T-Activator CD3/CD28 (25 ul/0.5 million cells; Life Technologies), along with IL-2 (30 U/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... H1299 (human non-small cell lung carcinoma, ATCC CRL-5803) and HMGA2 KO cells were cultured in RPMI (GIBCO) supplemented with 10% FBS (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... The THP-1 (human monocytic cells) cells were grown on Roswell Park Memorial Institute (RPMI) medium (Gibco, CA, USA) supplemented with 10% FBS and 0.05 mM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: 40-80 ng of each probe (labeled with biotin or Dig) and 1µg of unlabeled human COT1-DNA (Invitrogen) were dissolved in 4µl of hybridization buffer (10% dextran sulfate ...
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bioRxiv - Genomics 2021Quote: Human chronic myelogenous leukemia K562 cells (ATCC CCL-243) were cultured in RPMI 1640 Medium with GlutaMAX (Gibco 61870127), supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Genetics 2020Quote: ... HeLa and Human foreskin fibroblast (HFF) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FBS (Gibco). N2a cells were maintained in 45% DMEM and 45% Opti-MEM supplemented with 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: The AGS human gastric epithelial cell line (ATCC CRL-1739) was maintained in DMEM/F-12 (Thermo Fisher Scientific) containing 10% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... and Human pancreatic epithelial cells (hTERT-HPNE, ATCC, CRL-4023) were cultured in pyruvate-free DMEM (Gibco, 11965-092) supplemented with 10% FBS and P/S ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA from native pig and human lung tissue (fresh snap-frozen) was isolated using the PureLink Mini Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Synthetic genes encoding for human merlin FERM (22-312) and radixin FERM (5-295) domains were commercially synthesized (ThermoFisher) and cloned into pETM33 using NcoI and EcoRI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: ... The peptide–mRNA fusions were then incubated with human and yeast DUBm-immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Human ASGR1 and its mutants (Q240A/W244A and Q240A/W244A/E253A) and human ASGR2 were sub-cloned into pcDNA 3.1 vector (Invitrogen) containing a C-terminal Flag tag (ASGR1 ...
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bioRxiv - Synthetic Biology 2021Quote: Flp-In™ T-REx™ Human Embryonic Kidney (HEK) 293 cells were purchased from Thermo Scientific (Catlog# R78007). Cells were cultured in a humidity-controlled incubator under standard culture conditions (37°C with 5% CO2 ...
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bioRxiv - Developmental Biology 2021Quote: A human episomal iPSC line derived from CD34+ cord blood was used in this study (A18945, ThermoFisher Scientific; [58]). This cell line has already been validated in its ability to generate light-sensitive 3D retinal organoids ...
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bioRxiv - Immunology 2021Quote: ... This was followed by addition of alkaline phosphatase-conjugated goat anti-human F(ab’)2 antibody (Thermo Fisher Scientific) Inc. ...
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bioRxiv - Immunology 2021Quote: Cells from the human cancer cell line Colo205 were cultivated in complete RPMI-1640 medium (Thermo Fisher Scientific, USA) supplemented with 2 mM L-glutamine ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Caco-2 human IECs (ATCC #HTB-37) were grown in Dulbecco’s Modified Eagle Medium (DMEM; #11965, Gibco-ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Caco-2 human IECs (ATCC #HTB-37) were grown in Dulbecco’s Modified Eagle Medium (DMEM; #11965, Gibco-ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: The viral-integration-free human iPSC line generated using cord blood-derived from CD34+ progenitors was obtained from Gibco™/Thermo Scientific (Cat ...
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bioRxiv - Physiology 2021Quote: ... slgA concentrations in saliva were measured using the ready-set-go ELISA system for human IgA (Affymetrix: eBioscience, USA) with a sample dilution of 1:7500 at the University Hospital RWTH Aachen (SI ...
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bioRxiv - Cancer Biology 2020Quote: Human T-ALL cell lines (ARR, DU.528, HSB2, and CCRF-CEM) were cultured in RPMI 1640 medium (Gibco), supplemented with 10% heat inactivated Foetal bovine serum (HI-FBS ...
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bioRxiv - Evolutionary Biology 2021Quote: Human ESCs (WA01, also called H1, from WiCell Research Institute) were cultured with StemFlex Medium (Gibco, Cat. No. A3349401) in a feeder cell-free condition ...
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bioRxiv - Cancer Biology 2021Quote: Human breast cancer cell line MDA-MB-468 cells were cultured in Leibovitz L-15 medium (Gibco #21083-027) supplemented with 10% heat-inactivated FBS and 1xpenicillin/streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... was introduced to primary human umbilical vein endothelial cells (HUVEC) using the Neon® transfection system (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: Human telomerase-immortalised retinal pigment epithelial cells (hTERT-RPE1, ATCC) were grown in DMEM-F12 (Life Technologies, Paisley, UK) supplemented with 10% decomplemented FCS (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... Human alpha thrombin (Nordic Diagnostica AB, Billdal, Sweden) was resuspended in OptiMEM reduced serum medium (RSM) (Thermo Fisher Scientific) to 1 U/μl and stored at −20 °C ...
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bioRxiv - Biochemistry 2020Quote: The human colon cancer cell line HCT 116 was grown in McCoy’s 5A medium with glutamax (GIBCO, 36600–021) to which 10% fetal calf serum (FCS ...
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bioRxiv - Bioengineering 2021Quote: ... Briefly, THP-1 human monocytes (ATCC®, Manassas, VA) were cultured in complete RPMI media (ThermoFisher Scientific, Waltham, MA), supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin (37) ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h before opsonization with human IgG or 200 µg/mL anti-BSA antibody (Invitrogen, Burlington, ON, Canada). RAW cells were pre-cooled to 4°C for 5 min prior to the cells being presented with targets at ratios of 20-400 rods per cell ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma cells (HCT-8 cells; ATCC CCL-244) were cultured in RPMI 1640 medium (Gibco, ATCC modification) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 isolates (Human/NL/Lelystad/2020) were propagated in Vero E6 cells (ATCC) in Eagle’s minimal essential medium (Gibco) supplemented with 1% L-glutamine ...
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bioRxiv - Microbiology 2020Quote: ... Asexual parasites were cultured in human blood (UK National Blood Transfusion Service) and RPMI 1640 medium containing 0.5% w/v AlbumaxII (Invitrogen) at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Anti-human IgM and IgG conjugates provided within the kit were replaced with anti-mouse IgM-HRP-labeled (Invitrogen) and anti-mouse IgG-HRP-labeled (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
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bioRxiv - Immunology 2020Quote: ... human embryonic kidney cells (HEK) 293T were grown in a T75 flask with Dulbecco’s modified eagle medium (DMEM) (Gibco) + 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... gondii tachyzoites were maintained by growth on monolayers of human foreskin fibroblasts (HFF) in Dulbecco’s Modified Eagles Medium (DMEM) containing 10% Cosmic Calf Serum (GIBCO), 2 mM Glutamine ...
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bioRxiv - Immunology 2020Quote: ... the fixed sample was incubated with 1:500 dilution of goat anti-human coupled to Alexa 633 fluorophore (Invitrogen) and incubated for 30 min at RT ...
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bioRxiv - Immunology 2021Quote: Cytokines in AqH samples were quantified using a ProcartaPlex Human Cytokine-Panel 1B (Thermo Fisher Scientific, Waltham Massachusetts, USA) that quantifies ...
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bioRxiv - Immunology 2021Quote: Human CD4 T cells were MACS-sorted from PBMC and subsequently pre-activated with α-CD3/CD28 Dynabeads (Thermofisher) at a bead-to-cell ratio of 1:1 for 48 hours ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed and 50 μL of a secondary Alexa Fluor 647 goat anti-human antibody (Life Technologies A21455) (1:500 in PBS with 0.1% (w/v ...
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bioRxiv - Immunology 2020Quote: A CRISPR plasmid targeting human Hk2 was generated using the GeneArt® CRISPR Nuclease Vector Kit from Thermo Fisher. Caco-2 cells were purchased from DSMZ (ACC-169 ...
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bioRxiv - Genomics 2021Quote: ... Naive and memory T cells were then stimulated with anti-CD3/anti-CD28 human T-Activator Dynabeads® (Invitrogen) at a 1:2 beads-to-cells ratio ...