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Citations for Thermo Fisher :
6051 - 6100 of 7736 citations for Recombinant Human SRPK1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: The HaCaT cell line (Human adult low Calcium high Temperature) were grown in Dulbecco’s Modified Eagle Media (DMEM, Invitrogen) supplemented with 2 mM essential amino acids (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Proliferation was triggered by the stimulation with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific). The arginase inhibitor OAT-1746 (1500 nM) ...
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bioRxiv - Genetics 2021Quote: ... Fetal cortex samples were acquired from the MRC-Wellcome Trust Human Developmental Biology Resource (HDBR) (http://www.hdbr.org/) in Hibernate-E media (ThermoFisher Scientific). All samples were obtained through elective terminations of pregnancy ...
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bioRxiv - Microbiology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors by performing a Ficoll-Paque gradient (Thermo Fisher) on whole blood ...
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bioRxiv - Immunology 2020Quote: ... Samples were thawed on ice and prepared according to the manufacturer’s instructions using a custom-made 33-plex human cytokine panel (Procartaplex ThermoFisher). In brief ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: The screening was performed with a genome-wide siRNA library (Ambion SilencerR Human Genome siRNA library v3, Thermo Fisher), composed of siRNAs targeting approximately 21000 genes arrayed on 384-well plates ...
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bioRxiv - Molecular Biology 2021Quote: ... kidney) and HEK293T (Human embryonic kidney) cell lines were cultivated in Dulbecco’s modified Eagle (DMEM) (Thermo Scientific #12100-046) medium ...
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bioRxiv - Microbiology 2021Quote: Immortalized human vaginal epithelial cells (VK2/E6E7, ATCC CRL-2616) were cultured in keratinocyte serum-free medium (KSFM) (Gibco) with 0.5 ng/mL human recombinant epidermal growth factor and 0.05 mg/mL bovine pituitary extract ...
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bioRxiv - Neuroscience 2022Quote: Human iPSCs (male WTC11 background, PMID 24509632) were cultured in StemFlex™ Basal Medium (Gibco; Cat. No. A33493-01) on BioLite Cell Culture Treated Dishes (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Human female osteosarcoma U2OS and breast cancer MCF7 cells were cultured in DMEM + Glutamax (Thermo Fisher Scientific, 31966-047) supplemented with 10% FCS and 1% Penicillin/Streptomycin ...
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bioRxiv - Neuroscience 2021Quote: Primary hippocampal neurons were transfected with 1.5 μg human α-synuclein-GFP plasmid DNA [48] using lipofectamine-LTX (Thermofisher) on DIV6 and imaged on DIV10 using an inverted ZEISS Z1 Cell Observer fitted with a Colibri2 cool LED 5-channel fast wavelength switching system ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA expression analysis was performed with Affymetrix Human ClariomS® microarray using the WT Plus Reagent kit (ThermoFisher Scientific). As starting material ...
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bioRxiv - Immunology 2021Quote: ... The nanoparticles were resuspended in DPBS++ containing 1μM biotin-labeled anti-human kappa light chain (Igκ) (CaptureSelect; Thermo Fisher), and incubated 20min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... mouse fibroblast NIH-3T3 L929 cells and human embryonic kidney HEK 293 cells were grown in DMEM medium (Dulbecco’s Modified Eagle Medium, Gibco) supplemented with sterile-filtered 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... MDCK-CMAH cells were prepared by transfection of the human CMAH gene in a plasmid under the control of the CMV promoter (pcDNA3.1, Invitrogen). Clones with Neu5Gc expression were determined by HPLC analysis as previously described (63) ...
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bioRxiv - Biochemistry 2019Quote: ... Human WT (GM03652), NPC1 (GM03123), and NPC2 (GM18455) fibroblasts (Coriell Institute, Camden, NJ) were maintained in DMEM media (Invitrogen) supplemented with 15% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Immunology 2019Quote: ... and human bronchial carcinoma Calu3 epithelial cells (ATCC_HTB-55) were grown in 10% FCS in alpha MEM media (Gibco). Human alveolar epithelial carcinoma A549 epithelial cells (ATCC_CCL-185 ...
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bioRxiv - Microbiology 2019Quote: ... and C were selected and designed using MEGA6 software [30] and evaluated by the Thermofisher custom genomic TaqMan assay and design pipeline for non-human templates (https://www.thermofisher.com/order/custom-genomic-products/tools/genotyping) and analyzed using the ABIprism 7900HT (Applied Biosystems): SpaP SNP2137-2139 [GT/CG] ...
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bioRxiv - Genomics 2019Quote: ... The mouse and human RNA samples were globin-depleted using the species-specific Ambion® GLOBINclear kit (Ambion, Thermofisher) and the RNA was quantified using a NanoDrop One spectrophotometer (Thermofisher).
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bioRxiv - Genomics 2019Quote: ... The mouse and human RNA samples were globin-depleted using the species-specific Ambion® GLOBINclear kit (Ambion, Thermofisher) and the RNA was quantified using a NanoDrop One spectrophotometer (Thermofisher).
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bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
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bioRxiv - Immunology 2020Quote: ... The human monocytic THP-1 cell line was maintained in RPMI-1640 (made inhouse, RPMI 1640 powder (Life Technologies), 23.8mM Sodium Bicarbonate (NaHCO3 ...
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bioRxiv - Cell Biology 2020Quote: ... Data were processed by database searching against Human Uniprot Proteome database using Proteome Discoverer 2.3 software (Thermo Fisher Scientific). Precursor and fragment mass tolerance were set at 10 ppm and 0.6 Da respectively ...
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bioRxiv - Genetics 2019Quote: ... to distinguish recent and distant relatedness in the sample using markers from the Genome-Wide Human SNP 6.0 array (Affymetrix). The percent of total variation in height and BMI was estimated using the coefficient of determination (R2 ...
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bioRxiv - Developmental Biology 2020Quote: Human iPS cells (iPSCs, ATCC-DYS0100) were maintained in self renewal on a layer of geltrex (Gibco, A14133-01), in E8 Basal Medium (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Unlabeled and ferumoxytol-labeled human MSCs were tested for tri-lineage differentiation potential using StemPro differentiation kits (Life technologies) for adipogeneic ...
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bioRxiv - Genomics 2019Quote: ... The HUES9 human embryonic stem cell line was grown on tissue culture plates coated wth Geltrex (Thermo Fisher Scientific) in mTeSR1 media (StemCell Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... Data were processed by database searching against Human Uniprot Proteome database using Proteome Discoverer 2.2 software (Thermo Fisher Scientific). Precursor and fragment mass tolerance were set at 7 ppm and 0.6 Da respectively ...
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bioRxiv - Developmental Biology 2019Quote: DNA sequences corresponding to the human Amyloidβ1-42 amino acid sequence were synthesized (IDT) into a Gateway technology (Invitrogen) entry vector ...
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bioRxiv - Microbiology 2019Quote: ... and human glioblastoma cells (U-87MG, ATCC no.: HTB-14) were propagated in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies). Chinese hamster ovary cells (CHO-K1 ...
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bioRxiv - Cancer Biology 2020Quote: ... The labeled samples were then hybridized to Affymetrix Human Genome U133 Plus 2.0 Array GeneChips (ThermoFisher Scientific, Waltham MA) and scanned Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA from sorted CXCR3+ or CXCR3− SUM-LM1 cells was analyzed using Affymetrix Human U133 Plus 2.0 microarrays after cDNA preamplification using the 3’IVT Pico Reagent Kit (Affymetrix). To generate the CXCR3 signature ...
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bioRxiv - Cancer Biology 2019Quote: The immortalised non-tumorigenic human epithelial cell line MCF12A (ATCC, CRL-10782),90 were cultured in DMEM/F12 (Gibco) supplemented with 5% horse serum (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... Swabs were screened for common human respiratory pathogens using a customized TaqMan Array Card® (ThermoFisher, Waltham, MA, USA), which included influenza A and B viruses ...
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bioRxiv - Immunology 2021Quote: ... NAFs/CAFs ± SI were added to wells of lymphocytes at a concentration of 1×104 cells/100μl (ratio of 1:10 NAFs/CAFs:lymphocytes) of human complete MSC medium (RPMI 1640 medium (ThermoFisher Scientific) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney (HEK)-293 cells (ATCC, CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (Gibco, 11965-092) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2020Quote: Fresh-frozen human postmortem brain tissues were sectioned at 14-16 μm onto Superfrost Plus glass slides (Fisher Scientific). Sections were dried for 20 minutes at −20°C and then vacuum sealed and stored at −80°C until use ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human T cells and the Jurkat.E6 cell line were cultured in RPMI-1640 (Thermo Fisher Scientific; catalog #11875119). All media were supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Biochemistry 2020Quote: Cas9 was produced by IVT using either a continuous exchange 1-Step Human Coupled IVT Kit (Thermo Fisher Scientific) or a PURExpress bacterial IVT kit (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... Human parental HEK293 cells (ATCC, CRL-1573) were cultured in DMEM supplemented with 10% FBS and antibiotics/antimycotics (Gibco).
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bioRxiv - Neuroscience 2020Quote: Human sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and coverslips were mounted using Prolong Gold (Invitrogen) for imaging ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... cells were isolated from human foreskin tissue and cultivated at 37 °C and 5% CO2 in Eagle’s minimal essential medium (Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... Stealth RNAiTM siRNAs Negative Control, Med GC and human Il11, NM_000641.3 (HSS179893, HSS179894, HSS179895) were purchased from Thermo Fisher Scientific.
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bioRxiv - Biochemistry 2021Quote: DNA sequence coding for human Xkr8 (hXkr8) (UniProt: Q9H6D3) was custom-designed and synthesized by GeneArt (Thermo Fisher Scientific) to enhance mRNA stability and translational efficiency ...
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bioRxiv - Bioengineering 2020Quote: ... and MDA-MB-231 (ATCC HTB-26) human breast cancer cells were cultured in medium composed from standard Dulbecco’s Modified Eagle Medium (DMEM, Gibco), 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2021Quote: ... Human ACE2 ectodomain (NCBI Reference Sequence: NP_001358344.1, residues Q18-S740) was expressed in Expi293™ cells (Thermo Fisher Scientific) with a preceding murine IgG1 signal peptide ...
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bioRxiv - Biochemistry 2021Quote: ... Wild type/native human basigin (BSG-2, residues M1-L206) was expressed in Expi293™ cells (Thermo Fisher Scientific) with C-terminal rat CD4 domains 3+4 (CD4d3+4 ...
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human Alexa-647 [Cat# A21445] and streptavidin-AlexaFluor 635 [Cat# SA1011] antibodies were obtained from Thermo Fisher. Control lectins Erythrina cristagalli agglutinin (ECA ...
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bioRxiv - Neuroscience 2021Quote: Human neuroglioma cells (H4) were maintained in Opti-MEM I Reduced Serum Medium (Life Technologies- Gibco, Carlsbad, CA, USA) and Human Embryonic Kidney 293 (HEK ...
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bioRxiv - Neuroscience 2021Quote: Human neuroglioma cells (H4) were maintained in Opti-MEM I Reduced Serum Medium (Life Technologies- Gibco, Carlsbad, CA, USA) and Human Embryonic Kidney 293 (HEK ...