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Citations for Thermo Fisher :
6151 - 6200 of 6212 citations for MORF4L2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing gRNA with no sequence homology to any region of the human genome (LentiArray™ CRISPR Negative Control Lentivirus, Thermo Fisher Scientific, Cat # A32327).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Epi-Life® Growth Medium with 60 µM calcium and Cascade Biologist human keratinocyte growth supplement (HKGS) 100X (S-001–5) were from Life Technologies (Grand Island, NY, USA). The Dulbecco’s phosphate-buffered saline (DPBS) ...
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bioRxiv - Immunology 2022Quote: ... operated by UNICORN 6.4, using HiTrap Protein A columns (Cytiva, catalog no. GE17-5079-01) for human IgG1 and CaptureSelect™ CH1-XL (Thermo Fisher, catalog no. 494346201) for Fab fragments ...
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bioRxiv - Cancer Biology 2019Quote: ... and the GTP-loaded Rac pulled down from the total protein lysates using a Rac1 Activation Kit (GST-human Pak1-PBD, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: The concentration of sPD-1 and sTim-3 was measured in thawed plasma samples by enzyme linked immunosorbent assay (ELISA) using Human PD-1 (PDCD1) ELISA kit [EHPDCD1] (Thermo Fisher Scientific, Waltham, MA USA) and Quantikine ELISA Human TIM-3 Immunoassay kit [DTIM30] (R&D Systems ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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bioRxiv - Cancer Biology 2019Quote: ... was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). In vitro experiments with KJON cells were performed with 2 % HS in RPMI and IL-6 (1 ng/mL) ...
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: ... HT29-MTX cells were transiently transfected with 50 µM silencing RNA (siRNA) specific for human p32 (exon 3; s2138; Thermo Fisher Scientific, Waltham, Massachusetts, US) or control siRNA (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression profiling of the kidney biopsies was previously carried out using Affymetrix GeneChips (HumanGenome U133 Array and U133Plus2 Array and Affymetrix Human Gene ST GeneChip 2.1) as reported (35,36) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... parasites were maintained in vitro essentially as described previously,12 in human O- erythrocytes suspended in HEPES- and GlutamaxTM-supplemented RPMI 1640 (Thermo Fisher Scientific, order number 72400120) to which 11 mM D-glucose ...
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bioRxiv - Microbiology 2020Quote: ... S100 Calcium-binding protein A7 (S100A7) and human β-defensin 2 (DEFB4A) were amplified by using TAQMAN Gene Expression Assays (Applied Biosystems, Foster City, CA). TaqMan probes were grafted with a fluorophore (FAM ...
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bioRxiv - Cell Biology 2019Quote: ... 24 aliquots of human blood (20 μL) from each donor were pipetted into 1 mL 2D-barcoded tubes (Matrix Storage Tubes; Thermo Fisher Scientific Inc., Waltham, MA), placed into a ANSI/SLAS microplate format compatible 96-tube racks (8 × 12 ...
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bioRxiv - Developmental Biology 2020Quote: ... the ells were transfected with human EC-SOD (hEC-SOD) cDNA inserted into a vector plasmid pcDNA3 (5446 nucleotides; Invitrogen Life Technologies, Carlsbad, CA, USA) as previously described,14 using the FuGENE kit (Roche Diagnostics ...
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bioRxiv - Immunology 2020Quote: ... Immune mediator levels in COVID-19 patient plasma samples across acute samples were measured with by Luminex using the Cytokine/Chemokine/Growth Factor 45-plex Human ProcartaPlex™ Panel 1 (ThermoFisher Scientific, #EPX450-12171-901). Data acquisition was performed on FLEXMAP® 3D (Luminex ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Immunology 2021Quote: ... as well as the human lung epithelial Calu-3 cells (ATCC® HTB-55™) were maintained in Dulbecco modified Eagle medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS) ...
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bioRxiv - Immunology 2022Quote: We isolated T cells from PBMC of patients and healthy controls for autologous mixed lymphocyte reaction (MLR) assay using Dynabeads Untouched Human CD4+ T Cells kit (Invitrogen Dynal cat. No.113.52D, US). For MLR ...
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bioRxiv - Cancer Biology 2022Quote: ... the RNAi screen targeting 10,415 druggable genes (three individual siRNAs per gene) was conducted using OV90 cells and the Silencer® Select Human Druggable Genome siRNA Library Version 4 (Ambion Thermo Fisher Scientific, Waltham, MA), in absence or presence of bardoxolone methyl ...
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bioRxiv - Neuroscience 2023Quote: ... and growth factors was performed using a customized multiplex bead-based immunoassay - Immune Monitoring 65-Plex Human ProcartaPlex™ Panel (#EPX650-10065-901; ThermoFisher Scientific, Waltham, MA, US) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Pathology 2024Quote: ... primary dermal fibroblasts (normal, human, adult; PCS-201-012, ATCC, VA, USA) were cultured in Dulbecco’s Modified Eagle medium (DMEM) (Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Pathology 2023Quote: Immunohistochemical staining for human claudin-2 was performed on formalin fixed paraffin embedded human pancreas tissue sections by using a mouse-anti claudin-2 (1:100, Invitrogen Cat# 32-5600, RRID: AB_2533085). Pancreas sections were deparaffinized ...
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bioRxiv - Immunology 2024Quote: The amounts of TNF-α and IL-6 in culture supernatants of LPS or Pam3cys-re-stimulated IS-trained macrophages were quantified using commercial human ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). Optical density was measured using the Infinite M200 (Tecan ...
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bioRxiv - Immunology 2022Quote: ... from normal donors were obtained with an NIH-approved protocol and activated with CD3 and CD28 microbeads at a ratio of 1:3 (Dynabeads Human T-Expander CD3/CD28, Thermo Fisher Scientific, catalog no. 11141D) in AIM-V media containing 40 IU/mL recombinant IL-2 and 5% FBS for 24 hr ...
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bioRxiv - Immunology 2023Quote: ... HBEC3-KT cells were cultured in keratinocyte serum-free medium supplemented with human epidermal growth factor and bovine pituitary extract (Thermo Fisher Scientific, Grand Island, NY). MLE-15 cells were cultured in DMEM/F2 medium supplemented with 2% of fetal bovine serum and 0.5% of Insulin-Transferrin-Selenium (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Single-cell suspensions of the human iPSC-derived lung organoids were treated with 1 × Permeabilization Buffer (e-Bioscience, Thermo Fisher Scientific, Cat# 00-8333-56), and then incubated with APC-labeled anti-human HLA class 1 (R&D systems ...
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bioRxiv - Immunology 2023Quote: ... were seeded in Ultraculture complete medium and partially activated with dynabeads human T-activator CD3/CD28 (final dilution 1:1000) (Thermo Fisher Scientific, Waltham, MA USA) or completely activated with dynabeads human T-activator CD3/CD28 and IL2 2.5 U/ml ...
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bioRxiv - Neuroscience 2023Quote: ... which specifically targets on the phosphorylated Thr188 of murine C/EBPβ (a conservative locus to Thr235 of human C/EBPβ) in eBioscience™ Transcription Factor Staining Buffer Set (Invitrogen, Catalog #00-5523-00). For background signal staining ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cell Biology 2023Quote: NHK/38 cells and sections of normal human epidermis were stained with a monoclonal anti-hIL-38 antibody (H127C) (14-7385-82, Thermo Fisher Scientific AG, 1/2000), followed by an Alexa Fluor 594-labeled goat anti-mouse IgG2b (115-585-207 ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Prior to analyses ...
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bioRxiv - Genomics 2023Quote: ... 5 U/mL, human Chorionic Gonadotropin-hCG (Chorulon®, Intervet International B. V., Boxmeer, Netherland) and 50 μg/mL gentamicin (Gibco Thermo Fisher, Massachusets - USA). Culture droplets were covered with mineral oil and placed in an incubator at 38.5°C and a humid atmosphere of 5% CO2 in atmospheric air ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary NK cells were enriched by negative selection using the Invitrogen™ Dynabeads™ Untouched™ Human NK Cells Kit (Thermo Fisher Scientific, cat# 11349D) and expanded in Gibco™ CTS™ NK-Xpander™ Medium (Gibco™ ...
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bioRxiv - Neuroscience 2019Quote: ... Amplification of mRNA was performed by using TaqMan probes for human or murine specific α-synuclein and for selected cytokines/chemokines (Applied Biosystems Europe B.V., Zug, Switzerland). Target mRNA was normalized to tissue-specific murine GAPDH levels and displayed as relative expression after 30 amplification cycles.
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bioRxiv - Bioengineering 2020Quote: ... Bone marrow human macrophage (KG-1 ATCC® CCL246™) passages 2–5 were cultured in cell suspension flasks using IMDM medium (Gibco™ GlutaMAX™, HEPES) with 20% FBS ...
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bioRxiv - Cell Biology 2019Quote: ... The samples were then rinsed with PBS (5 × 3 min) and incubated for 1 h at 37°C with the following secondary antibodies: anti-human Alexa 488 (Thermo Fisher, Waltham, MA, USA; 1:750) or anti-mouse Alexa 488 (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... CHO-K1 cells constitutively expressing human KCNE1 (designated CHO-KCNE1 cells) were generated as previously described (74) using the FLP-in™ system (Thermo Fisher Scientific, Waltham, MA, USA) and maintained under selection with hygromycin B (600 μg/mL).
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bioRxiv - Immunology 2021Quote: To investigate a link between fkbpl gene expression and psoriasis a search was performed on the NCBI GEO database to obtain mRNA microarray results and identified GSE14905 from skin biopsy samples (Affymetrix Human Genome U133 Plus 2.0 Array) which compared a non-disease control cohort and a diseased cohort ...
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bioRxiv - Immunology 2022Quote: ... samples were resuspended in binding buffer containing a 1:100 dilution of mouse anti-human-IgG1-Fc conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, Rockford, IL; A-10631) and incubated for 30 minutes at room temperature in the dark ...
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bioRxiv - Cancer Biology 2019Quote: The Kaplan–Meier plotter was used to cross-validate the effect of differentially expressed genes in an independent ovarian cancer cohort (Geo accession identifier: GSE9891).31 This replication cohort included gene expression profiling (Affymetrix Human Genome U133 Plus 2.0 Array) of 285 ovarian tumor samples ...
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bioRxiv - Molecular Biology 2019Quote: ... falciparum or human A549 total RNA was enriched by two successive rounds of purification with the Dynabeads mRNA purification kit (Thermo Fisher # 61006, Supplementary Fig. 1b,c).
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from the six EBV-induced lymphomas (tumor group) and normal human lymphocytes (normal group) using TRIZOL reagent (Ambion/Life Sciences, Grand Island, NY, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells used for transduction were obtained by magnetic separation from healthy-donor derived apheresis products (Dynabeads® Untouched™ Human CD8+ T Cells Kit, Thermo Fisher; Magnet, DynaMag™).
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bioRxiv - Immunology 2021Quote: ... as well as the human lung epithelial Calu-3 cells (ATCC® HTB-55™) were maintained in Dulbecco modified Eagle medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS) ...
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bioRxiv - Cancer Biology 2022Quote: ... the RNAi screen targeting 10,415 druggable genes (three individual siRNAs per gene) was conducted using OV90 cells and the Silencer® Select Human Druggable Genome siRNA Library Version 4 (Ambion Thermo Fisher Scientific, Waltham, MA), in absence or presence of bardoxolone methyl ...
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bioRxiv - Immunology 2022Quote: ... the cells were first extracellularly stained with a mouse anti-human monoclonal antibody APC-Cy7 conjugated LIVE/DEAD™ Fixable Near-IR Dead Cell Stain (#L10119, Invitrogen, Thermo Fischer Scientific, OR, USA), following a similar preparation procedure as just described for the DCs ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated with 100 ng/μL of mouse anti-human CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, eBioscience/Thermo Fisher Scientific; Catalog # 13-1719-82); mouse anti-human GLAST (ACSA-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Evaluation of proliferation in tissues using antibodies against Ki67 or BrdU (anti-human Ki67 and anti- BrdU monoclonal antibodies; Thermo-Fisher Scientific and BD BioSciences, USA, respectively) was performed as previously described {GarciaMelendrez:GKcY05su } ...
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bioRxiv - Cell Biology 2023Quote: ... HUVECs were grown to ∼80% confluence followed by transfection with 100 nM human ERG Silencer Select (ID #s4811) or nontargeting siRNA oligos (Life Technologies: ERG #4392420 and nontargeting #AM4635) using lipofectamine RNAiMAX (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Transient genetic silencing of HOIP and FLOT1/2 was performed by reverse transfection of HT-29 cells with the following siRNAs: human siHOIP (RNF31) (Silencer® Select siRNA #1 s30108, #2 s30109, #3 s30110, 40 nM) (Thermo Fisher Scientific, Waltham, MA, USA), human FLOT1 (ON-TARGETplus Human FLOT1 siRNA SMART POOL ...