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Citations for Thermo Fisher :
5751 - 5800 of 6212 citations for MORF4L2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and the corresponding light chain (human IgKappa, IgLambda) expression vectors respectively and produced in transiently transfected ExpiCHO-S cells (Thermo Fisher, #A29133) at 37°C and 8% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... Recovered nanovials were washed two times with washing buffer and sequentially labeled with streptavidin and biotin goat anti-human IgG Fc (Thermo Fisher, A18821) following standard procedures described above ...
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bioRxiv - Cancer Biology 2021Quote: ... together with 0.5µg of a plasmid expressing the renilla luciferase under the control of the human β-actin promoter using Lipofectamine 3000 (ThermoFisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Human VAT and SAT biopsies (30-50g) were minced and digested with 3mg/mL type II collagenase solution (ThermoFisher Scientific Inc./Gibco) in HBSS containing calcium chloride ...
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bioRxiv - Microbiology 2022Quote: ... falciparum B11 line (Perrin et al., 2018) was maintained at 37° C in human RBCs in RPMI 1640 containing Albumax II (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine ...
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bioRxiv - Microbiology 2022Quote: His-tagged soluble human ACE2 was produced in FreeStyle™ 293-F cells by transfecting soluble human ACE2 (1-741 aa) expression vector plasmid DNA using 293fectin (Thermo Fisher) and purified using HiTrap Chelating column charged with nickel (GE healthcare ...
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bioRxiv - Microbiology 2022Quote: APEX2 coding sequence (56) with the FLAG epitope at the N-terminus optimized for expression in human cells was synthesized by Invitrogen (GeneArt service). For the transient expression ...
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bioRxiv - Neuroscience 2022Quote: ... were placed in co-culture with astrocytes 24 h following purification and both cell types were maintained in human ESFM supplemented with B-27 (Thermo Fisher Scientific). Co-cultures were maintained for 24 h prior to experiments.
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bioRxiv - Immunology 2022Quote: A human primary fibroblast line was maintained in Dulbecco’s Modified Eagle’s Medium with 10% heat inactivated FBS (Thermo Fisher Scientific, Gothenberg, Sweden), 10 U/ml Penicillin/Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... arrays were incubated for 1h30 with AF647-conjugated goat anti-human IgG antibodies (at 1 µg/ml in PBS; Thermo Fisher Scientific), and revealed using GenePix 4000B microarray scanner (Molecular Devices ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated 20 min at 4°C with AF647-conjugated goat anti-human IgG antibodies (1:1000 dilution; Thermo Fisher Scientific) and LIVE/DEAD Fixable Viability dye Aqua (1:1000 dilution ...
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bioRxiv - Immunology 2022Quote: FLAG-DNAM-1-expressing human Treg cells were co-cultured in complete RPMI-1640 culture medium with 2 × 104 CellTrace Violet (Thermo Fisher Scientific)-labeled Tconv cells (freshly isolated from the same donor as that of the Treg cells ...
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bioRxiv - Cancer Biology 2022Quote: ... These plasma samples were diluted at least 2x or more (as required) and then were used to detect PTN by ELISA using commercial human PTN ELISA kits (ThermoFisher Scientific, EH370RB). CXCL5 ELISA from tumor lysates was performed using commercial kit (R&D Systems ...
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bioRxiv - Microbiology 2022Quote: ... 99 base pair DNA sequences from genes selected for validation were amplified from human RNA using SuperScript IV One-STEP RT-PCR (Thermo Fisher Scientific), or PCR amplified from human cDNA using Vent Polymerase (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... The internalization of the ACE2-HA protein and anti-hACE2 mAbs was then evaluated by staining with goat anti-mouse Alexa Fluor™ 594 (to detect the HA-tagged hACE2) and/or goat anti-human Alexa Fluor™ 488 antibodies (to detect the hACE2 mAbs) (ThermoFisher Scientific). Images were captured using a DeltaVision OMX SR imaging system (GE Healthcare).
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bioRxiv - Systems Biology 2020Quote: We cultured GM11169 human cardiac fibroblasts (Coriell, GM11169; XX donor) on tissue culture-treated dishes in DMEM w/Glutamax + 9% FBS (Life Technologies 16000044) + P/S.
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bioRxiv - Molecular Biology 2019Quote: ... the miRIDIAN microRNA Mimic (catalog number CS-001030) and Inhibitor (catalog number IH-001030) Libraries (19.0, human microRNAs) were purchased from Dharmacon (Thermo Fisher Scientific Inc.). For the mimic screen ...
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bioRxiv - Cancer Biology 2019Quote: ... labeled cDNA targets were generated with the Encore® Biotin Module (Nugen) and hybridized to a GeneChip® Human Transcriptome Array 2.0 (Affymetrix, Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... cells were cultured for 14 days in a humified CO2 incubator at 37°C in complete culture medium with Dynabeads® Human T-Activator CD3/CD28 beads (ThermoFisher Scientific) (25µl/well ...
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bioRxiv - Immunology 2019Quote: ... a 20X IL-26 human TaqMan® probe was used (Hs00218189_m1) with 2X TaqMan® Fast Advanced Master Mix (both from ThermoFisher Scientific). OSM and HEBGF cDNA quantification was performed with Roche ® hydrolysis probes (OSM ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA from human cytobrushes was also reverse transcribed to first strand cDNA using the SuperScript™ IV VILO™ Master Mix (11766050, Invitrogen). To quantify host inflammatory mediators’ transcripts (IL-6 ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with human PBMCs isolated from healthy donors that were fluorescently labeled with Cell Trace Violet (Invitrogen, Cat. Nr.: C34557) at an effector:target ratio of 20:1 ...
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bioRxiv - Microbiology 2021Quote: Primary HFFF immortalised with human telomerase (HFFF-TERTs) (96) were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Thermo Fisher Scientific, Lutterworth, UK) supplemented with 10 % foetal bovine serum (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Mouse lung fibroblasts (CCL-206, ATCC, Wesel, Germany) or human lung fibroblasts (MRC5, ATCC, CCL-171) were cultured in 1:1 DMEM (Gibco, MD, USA) and Ham’s F12 (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... The cells were washed three times with PBS and then incubated with either goat anti-human IgG conjugated with Alexa fluor 488 at a dilution of 1:500 for 1 h (Invitrogen, Carlsbad, CA). The cells were then washed and stained with hoechest-33342 (Invitrogen ...
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bioRxiv - Genetics 2020Quote: RNA was extracted from pulverized left or right ventricle human cardiac tissue using the mixVana miRNA isolation kit (Thermo Fisher Scientific, #AM1560), according to the manufacturer’s specifications ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... All coding exonic and flanking intronic regions of the human TP53 gene were amplified from genomic DNA with Platinum™ Taq DNA polymerase (Life Technologies) by multiplex PCR using two primer pools with 12 non-overlapping primer pairs each ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were transfected with 2.5 µM ON-TARGET plus human SAMHD1 siRNA SMART-pool obtained from Dharmacon (Munich, Germany, L-013950-01-0050) in resuspension electroporation buffer R (Invitrogen, Dreieich, Germany) using the Neon transfection system (Invitrogen ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... as well as cell culture supernatants were analyzed on a Luminex™ MAGPIX™ instrument with a custom human cytokine panel (ThermoFisher). Samples were thawed at room temperature and prepared according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Insertion of single base pair changes for the generation of human and mouse FOXN1 variants was achieved by site-directed mutagenesis using the Phusion site-directed mutagenesis kit (Thermo Fisher Scientific).Specific primers for the introduction of single base pair deletions or single base pair changes were designed using the following software www.agilent.com/store/primerDesignProgram.jsp ...
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bioRxiv - Immunology 2020Quote: ... Anti-IgE beads were created for use in the LIPS assay by coupling goat anti-human IgE (SeraCare KPL, Gaithersburg, MD) to Ultralink beads (ThermoFisher, Waltham, MA).
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bioRxiv - Neuroscience 2019Quote: ... or three helper plasmids (pCMV-VSV-G, pMDLg/pRRE, pRSV-Rev) in human embryonic kidney 293T (HEK293T) cells using Lipofectamine 2000 (Thermo Fisher Scientific) following the instructions of the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... was performed using Taqman reagents and specific primer and probe sets for human SLC22A24 (Assay ID: Hs00543210_m) and GAPDH (Assay ID: Hs99999905_m1) (Applied Biosystems, Foster City, CA). Reactions were performed in a 96-well plate with a 10 µL reaction volume using an ABI 7900HT Fast Real-time PCR system (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: Human embryonic stem cells and human induced pluripotent stem cells (hESCs and iPSCs, Table S1) were grown using StemFlex™ Medium (A3349401, Gibco™) supplemented with Normocin™ Antimicrobial Reagent (Invivogen ...
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bioRxiv - Microbiology 2021Quote: ... operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human mAbs and CaptureSelect CH1-XL MiniChrom columns (Thermo Fisher Scientific) for Fab fragments ...
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bioRxiv - Microbiology 2020Quote: ... They were then incubated with 1:200 diluted Alexa Fluor 594 conjugated goat anti-human IgG secondary antibody (Invitrogen, Thermo Fisher Scientific) for 45 min at RT and subjected to flow cytometric analysis with a CytoFLEX flow cytometer (Beckman) ...
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bioRxiv - Neuroscience 2021Quote: hiPSCs were previously generated from healthy adult human dermal fibroblast lines from a 32-year-old female from Invitrogen (C-013-5C), as described before (31) ...
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bioRxiv - Cell Biology 2019Quote: All human cells (listed in table 1) were cultured at 37°C in an atmosphere of 5% CO2 in DMEM (Thermo Fisher Scientific) supplemented with penicillin/streptomycin (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting NK1R-miniGs/q70 fusion construct was transfected into adherent Expi293F™ Inducible Human Embryonic Kidney Cells (unauthenticated and untested for mycoplasma contamination, Life Technologies) using Lipofectamine 2000Ω and cells were maintained in DMEM (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... The subsequent NK1R-miniGs399fusion construct was transiently transfected into 200-mLs of Expi293F™ Inducible Human Embryonic Kidney Cells (unauthenticated and untested for mycoplasma contamination, Life Technologies) using the Expifectamine Transfection Kit (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA; anti E-Cadherin antibody: CD324 #16-3249-82, Invitrogen, USA) to bind to their respective epitopes ...
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bioRxiv - Cell Biology 2021Quote: ... AlexaFluor488-labeled human transferrin was dissolved in PBS 5 at mg/ml and stored at 4°C (Thermo Fisher, ref. no. 13342). TexasRed-labelled neutral 3’000 and 40’000 Da dextran was dissolved in PBS at 10 mg/ml and stored at -20°C (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA deriving from LCM human pancreatic islets was then amplified using TaqMan PreAmp Master Mix (cat. 4488593, ThermoFisher Scientific, Waltham, MA, USA) following manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Vero-hSLAMF1 cells [52] stably express the human measles receptor SLAMF1 and were cultured in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific) containing 5% newborn calf serum (NCS ...
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bioRxiv - Immunology 2020Quote: ... The blocking of human mAb binding to pHA expressed on the surface of MDCK-SIAT1 cells was detected using second layer goat-anti-human IgG Alexa fluor 647 (Invitrogen A-21445) for 2-12C or streptavidin-Alexa fluor 647 (Invitrogen S21374 ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 ng of labelled probe was combined with 5 μg salmon sperm and 10 μg human C0t1 DNA (Invitrogen, Cat No 15279011). Two volumes of ethanol were added and the probe mix was collected by centrifugation and dried ...
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bioRxiv - Microbiology 2020Quote: ... HCT-8 human ileocecal colorectal adenocarcinoma cell line and its 5-FU-resistant was cultured in RPMI 1640 medium (Gibco, New Zealand) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2020Quote: ... The CF cell line CFBE41ō (ΔF508/ΔF508) and normal human bronchial epithelial cells16HBEō (from D. Gruenert) were maintained in complete MEM-media (GibcoBRL, Life Technologies, MD). The normal human tracheal cell line 9HTEō ...
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bioRxiv - Microbiology 2020Quote: ... we use 1µg/ml final concentration of ethidium bromide to stain parasitized erythrocytes and 4 µl/ml Alexa Fluor 488-conjugated goat anti-human IgG (H+L) (Invitrogen, Life Technologies), based on optimal signal to noise between positive control plasma (10 Mali highly exposed individual plasma ...