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Citations for Thermo Fisher :
6101 - 6150 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant containing nuclear proteins was measured for protein concentration using Pierce BCA Protein Assay Kit (Thermo Scientific) and stored at -80°C until use ...
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bioRxiv - Bioengineering 2023Quote: ... Total protein concentration was determined using the Qubit™ Protein and Protein Broad Range (BR) Assay Kit (Invitrogen).
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bioRxiv - Bioengineering 2023Quote: Protein yield: MSC-EV preparation total protein concentration was determined using the Micro BCA Protein Assay Kit (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration of each sample was determined using the BCA protein assay (Pierce™ BCA Protein Assay, Thermofisher). 40 micrograms of protein per lane were prepared in SDS sample buffer (50 mM Tris pH 6.8 ...
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bioRxiv - Neuroscience 2024Quote: Protein concentration in the protein sample was determined by using a BCA protein assay kit (Pierce, Thermo Scientific). Samples in aliquots of 100 μL each were stored at -80°C.
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bioRxiv - Genomics 2021Quote: ... Then 5 μg of each aRNA were retrotranscribed and labelled using a SuperScript II reverse transcriptase (Transcriptase inverse SuperScript™ II kit, Invitrogen, Carlsbad, CA, USA) and fluorescent dyes ...
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bioRxiv - Neuroscience 2020Quote: ... After that, 2 µl of RT mix (1x SuperScript II first-strand buffer, 18 U/µl SuperScript II reverse transcriptase (Life Technologies, Carlsbad, CA, USA), 3.6 µM C1-P1-RNA-TSO (5’-AAU GAU ACG GCG ACC ACC GAU NNN NNG GG -3’) ...
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bioRxiv - Genetics 2019Quote: ... and 2 unaffected individuals (II.1 and II.3) were analyzed with the Affymetrix GeneChip® Mapping 250K Array Xba 240 (Affymetrix, Santa Clara, CA). Sample processing and labelling were performed according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Then 5 µg of each aRNA were retrotranscribed and labelled using a SuperScript II reverse transcriptase (Transcriptase inverse SuperScript™ II kit, Invitrogen, Carlsbad, CA, USA) and fluorescent dyes ...
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bioRxiv - Microbiology 2020Quote: ... All PCR reactions were carried out using Recombinant Taq DNA Polymerase (5U/μl from Thermo Scientific) and standard PCR cycling conditions.
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bioRxiv - Cell Biology 2022Quote: ... Purified recombinant plasmid DNA was digested with PacI and transfected into HEK293 cells with Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Recombinant antibodies were transiently transfected and produced in vitro in 293F Freestyle suspension cells (Thermofisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... and recombinant expression was undertaken using the FreeStyle™ MAX 293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant GST-SHP2 in supernatant was affinity-purified by Pierce glutathione agarose (Thermo Fisher Scientific) and eluted with lysis buffer containing 20 mM GSH ...
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bioRxiv - Zoology 2021Quote: ... The recombinant plasmid was quantified using a NANO-DROP 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). The plasmid copy number was calculated using the equations described by Yun et ...
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bioRxiv - Plant Biology 2020Quote: ... Semi-quantitative PCR was performed within the linear amplification phase using a recombinant Taq Polymerase (Invitrogen) amplifying a fragment of 323 base pairs (bps ...
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bioRxiv - Cell Biology 2021Quote: ... The amplification reactions for qualitative PCR were performed using Recombinant Taq DNA polymerase (Invitrogen, 11615-038) according to the following parameters ...
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bioRxiv - Immunology 2021Quote: ... Recombinant bacmids encoding each RBDs were generated using the Bac-to-Bac system (Thermo Fisher Scientific) followed by transfection into Sf9 cells using FuGENE HD (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Genotyping PCR reactions were carried out using Recombinant Taq DNA Polymerase (5U/µl from Thermo Scientific) and standard PCR cycling conditions.
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bioRxiv - Bioengineering 2022Quote: ... Recombinant single-strand AAV2/9 vectors were produced by transfection of HEK293T cells (Thermo Fisher Scientific) with the respective pAAV expression plasmid ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant bacmids encoding each RBDs were generated using the Bac-to-Bac system (Thermo Fisher Scientific) followed by transfection into Sf9 cells using FuGENE HD (Promega ...
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bioRxiv - Immunology 2020Quote: ... the reaction mix was supplemented with 40 U RNaseOUT™ Recombinant Ribonuclease Inhibitor (10777019, ThermoFisher Scientific), 200 U SuperScript™ IV Reverse Transcriptase (18090050 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant RBD was diluted to 2.5 μg/mL in PBS (Thermo Fisher Scientific, Waltham, MA, USA) and 100 μl of the dilution was distributed in the wells of flat-bottom 96-well microplates (Immulon 2HB ...
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bioRxiv - Immunology 2020Quote: ... with oligo(dT) primers in the presence of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific) or using SuperScript™ IV VILO™ Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... and analysis of the recombinant bacmid were performed following the manufacturer’s instructions (ExpiSf Expression System, Gibco). ExpiSf9 cells were cultured in ExpiSf9 CD medium to a density of 5×106 cells/mL ...
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bioRxiv - Microbiology 2022Quote: ... BMDMs and MEFs were stimulated with 10 ng/mL (~100 U/mL) recombinant mouse IFNγ (Gibco) for 24 h prior to infection or left unstimulated ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant baculovirus was prepared using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific). Spodoptera frugiperda Sf9 insect cells (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: Recombinant GCase N370S mutant was produced by using 293T Freestyle cells (Thermo Fisher Cat. No. R79007) grown in suspension in 293 Freestyle medium (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... Dicer/TRBP enzyme was pre-mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... beads were incubated in kinase reaction buffer supplemented with 100 ng recombinant CAMK2D (Invitrogen, cat. PV3373), 100 µM recombinant calmodulin (Enzo ...
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bioRxiv - Genetics 2023Quote: ... and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen) for the PCR step using D388 specific primers 5′-GAGAGAAGGATTAGATTGTG-3′ and 5′-CCTGTATAATAGAAGTACCA-3′ ...
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bioRxiv - Evolutionary Biology 2023Quote: Recombinant PrPAP1Xa was secretively expressed using the Bac-to-Bac™ Baculovirus Expression System (Invitrogen, USA) as previously described22 ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: The recombinant baculovirus was prepared using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific). For expression ...
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bioRxiv - Microbiology 2024Quote: ... the recombinant section of the hisC::’ND5i’ allele was amplified by PCR using DreamTaq (Thermo Scientific) and primers hisC-ins-f/hisC-ins-r (Table 2) ...
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bioRxiv - Bioengineering 2024Quote: ... and 10 ng/mL recombinant Activin-A (338-AC, Bio-Techne) in RPMI 1640 (61870036, Gibco) with added B-27 supplemented minus insulin (12587010 ...
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bioRxiv - Biochemistry 2024Quote: The recombinant baculovirus was prepared using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific). For expression ...
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bioRxiv - Plant Biology 2020Quote: ... the Gateway LR clonase II enzyme mix (Thermo-Fisher Scientific) was used to recombine pENTR-ANAC017-GFP with the N-terminal tagging vector pUBN-RFP (Grefen et al. ...
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bioRxiv - Plant Biology 2020Quote: ... using LR clonase II (Thermo Fisher Scientific, Waltham, MA, USA). Similarly ...
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bioRxiv - Synthetic Biology 2021Quote: ... or Invitrogen Platinum Superfi II DNA Polymerase (Thermo Fisher Scientific). Expression vectors were created using classical restriction enzyme cloning or Golden-Gate assembly (40) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 mg/mL of the protease dispase II (Gibco) and gently rocked for 45 min at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... with 20 μL of Cellfectin II (Thermo Fisher, Cat. #10362100) and added to 1×106 adherent Sf9 cells ...
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bioRxiv - Cell Biology 2020Quote: ... and Platinum II Hot-Start Green PCR Master Mix (Invitrogen) according to kit instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Dispase II (Cat#17105041; Thermo fisher; used 15 U/mL) and Dnase I (Cat#DN25 ...
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bioRxiv - Cell Biology 2020Quote: ... Dispase II (Cat#17105041; Thermo fisher; used 15 U/mL), and Dnase I (Cat#DN25 ...
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bioRxiv - Cell Biology 2020Quote: ... XCell II™ Blot Module (#EI9051,Thermo Fisher Scientific, USA). Amersham ECL HRP-conjugated secondary donkey anti-rabbit antibody (#NA93V ...
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bioRxiv - Cell Biology 2020Quote: Cells were grown on LabTek II chamber slides (Thermo Scientific) for the indicated times and fixed with 4% paraformaldehyde at room temperature for 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... and counted with a Countess II automated cell counter (ThermoFisher). 250,000 cells were diluted in 2 mL with phenol red-free MEM and added to a quartz 2 mL cuvette ...
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bioRxiv - Immunology 2021Quote: ... the fusions were reverse transcribed with super script II (ThermoFisher). The pool was incubated with biotinylated SARS CoV2 Spike extracellular domain bound to 5 μL streptavidin M280 dynabeads (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... viable cell number was determined using the Countess II (ThermoFisher).