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Citations for Thermo Fisher :
6351 - 6400 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... on a Countess II FL Automated Cell Counter (Thermo Fisher). For BrdU incorporation studies ...
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bioRxiv - Microbiology 2023Quote: ... Total cDNA was amplified using Platinum SuperFi II polymerase (Invitrogen). After enzymatic cleanup ...
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bioRxiv - Genomics 2023Quote: ... and 10 U Superscript II Reverse Transcriptase (ThermoFisher 18064-071). The plates were placed in a thermocycler and incubated using the following program ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 µL of Platinum II Taq HS DNA pol (Invitrogen), and 29.8 µL of Ambion™ Nuclease-free water (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... cDNAs were obtained using Superscript II reverse transcriptase (Applied BioSystems) as recommended by the supplier ...
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bioRxiv - Genomics 2023Quote: ... and recombined using the LR Clonase-II Gateway System (ThermoFisher) into pGL4.23-GW (a gift from Jorge Ferrer ...
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bioRxiv - Developmental Biology 2023Quote: ... Superscript II reverse-transcriptase was replaced by SuperScript IV (Invitrogen) and cDNA clean-up was done using Omega Bio-Tek’s Mag-Bind TotalPure NGS ...
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bioRxiv - Developmental Biology 2023Quote: ... Super Script II (18064014, Thermo Fisher Scientific, Carlsbad, CA, USA) and actinomycin D ...
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bioRxiv - Cancer Biology 2023Quote: ... CLS-7 LS005332)/1 mg/mL Dispase II (Gibco, cat. no. 17105-041) on top of the tissue block and incubated it until the tissue lysis solution had frozen (∼3 min) ...
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bioRxiv - Developmental Biology 2023Quote: ... and counted with the Countess II (Life Technologies, cat#A27977). This procedure resulted in cell viability of at least 80% ...
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bioRxiv - Developmental Biology 2023Quote: ... and cDNA was synthesized with SuperScript II reverse transcriptase (Invitrogen) following the manufacturer’s instruction ...
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bioRxiv - Genomics 2023Quote: ... and reverse transcribed using Superscirpt II Reverse Transcriptase (Invitrogen 18064022) with random decamers ...
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bioRxiv - Biochemistry 2023Quote: Peptides were separated using EASY-nLC II nanoLC (Thermo Scientific) with an EASY-Spray Capillary HPLC Column (75 μm x 150 mm ...
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bioRxiv - Cancer Biology 2023Quote: ... and counted on a Countess II cell counter (ThermoFisher Scientific). Cells were resuspended in Hanks’ Balanced Salt Solution (ThermoFisher Scientific Cat# 88284 ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Gateway LR Clonase II enzyme mix (Invitrogen 11791020). Lentiviral particles were prepared by transfecting 293T cells with expression clones along with the lentiviral envelope and packaging plasmids using X-tremeGENE HP DNA transfection reagent (Sigma-Aldrich 6366546001) ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Gateway BP Clonase II Enzyme mix (Invitrogen 11789020). Expression vectors were generated by cloning into pInducer20 (gift from Stephen Elledge ...
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bioRxiv - Cancer Biology 2024Quote: ... The first strand cDNA was synthesized using Superscript II (Invitrogen) and random hexamers ...
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bioRxiv - Bioengineering 2024Quote: ... and direct electron detector (Falcon II 4K, Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... 8-well chambered coverglasses (Nunc Lab-Tek II, Thermo Scientific) were coated with rat tail collagen type I (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... the medium was replaced with 293 SFM II medium (Gibco) supplemented with Primatone 3.0 g/L (Kerry) ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of 0.5 mg/mL dispase II (Thermo Fisher, 17105041), 1 mg/mL collagenase IV (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... using a TaqMan™ Universal Master Mix II (Applied Biosystems). The gene expression levels were determined using the ΔΔCt method with the mRNA levels being normalized to the geometric mean of PPIA and B2M.
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bioRxiv - Bioengineering 2024Quote: ... 8-well chambered coverglasses (Nunc Lab-Tek II, Thermo Scientific) were coated with rat tail collagen type I (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2021Quote: ... The T cells were isolated using the EasySep human T cell isolation kit (Stemcell) and activated with human T-activator CD3/CD28 Dynabeads (Gibco) at a bead:cell ratio of 1:1 ...
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bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
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bioRxiv - Immunology 2022Quote: ... For Cytokine Multiplex of Human Inflammation Panel (Invitrogen™ Inflammation 20-Plex Human ProcartaPlex™ Panel) (Catalog # EXP20012185901, eBioscience/Invitrogen) procedures were performed following manufacturer protocol and the plate was processed on BioRad Bio-Plex 200 system with Bio-Plex-HTF attachment (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from human NSCLC cells and human clinical NSCLC tumor and normal samples by homogenization in Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... supernatants were diluted 4-fold and assayed with the Amyloid beta 40 Human ELISA Kit and either the Amyloid beta 42 Human ELISA Kit or the Amyloid beta 42 Human ELISA Kit Ultrasensitive (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: HMF and MRC5 fibroblasts were transfected with a pool of 3 siRNAs targeting each of the 710 human kinases (Silencer Human Kinase siRNA Library, #A30079 Thermofisher). Fibroblasts (1250 cells/well ...
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bioRxiv - Immunology 2019Quote: ... human PBMC derived T cells were purified by Human T cell isolation kit from Invitrogen (Invitrogen Dynal AS, Oslo, Norway) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were rinsed and re-suspended in flow buffer containing 25 μL mL−1 of each of the following monoclonal anti-human antibodies per sample: anti-human CD3-AF700 (56-0037-42, ThermoFisher), - CD40L/CD154-FITC (11-1548-42 ...
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bioRxiv - Immunology 2021Quote: ... and total T cells were purified from PBMC suspensions by negative immunomagnetic selection (purity >90%) using the Human Myeloid DC Enrichment Kit (STEMCELL) and the Untouched total human T cell (Invitrogen) kits ...
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bioRxiv - Cancer Biology 2022Quote: ... primary human breast CAFs were transfected with 75 nm human periostin-targeting stealth siRNA (si-POSTN) (ThermoFisher siRNA ID: HSS116400) or 75 nm non-targeting control siRNA (si-Control ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™ ...
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bioRxiv - Microbiology 2022Quote: ... Surface PCDH1 was stained using human anti-EC7 mAb-3677 (5 µg/mL) followed by anti-human Alexa FluorTM 555 (ThermoFisher) for 1 h at 4°C each ...
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bioRxiv - Microbiology 2022Quote: ... encoding full-length human MCT1 (uniprot ID: P53985) and human basigin (uniprot ID: P35613-2) were cloned individually into pFastBac vector (Invitrogen) for recombinant expression in Sf9 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... human ETAR (residues 21-406) and human ETBR (residues 27-424) DNA was cloned into a modified pFastBac vector (Invitrogen), which contains an N-terminal FLAG tag (DYKDDDD ...
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bioRxiv - Neuroscience 2023Quote: ... The human FUS or human TARDBP cDNA was subcloned into the pENTR™/D-TOPO® vector (Thermo Fisher Scientific). To generate the Gateway destination vector pUAST-DEST ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Neuroscience 2023Quote: ... and secreted signaling proteins were measured in culture supernatants by the Human Immune Monitoring Center (HIMC) at Stanford University using a Human 48-plex Luminex Procarta Immuno-assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: Human colorectal adenocarcinoma (ATCC, #SK809) and human pancreatic carcinoma (ATCC, #CRL1469) cells were grown in McCoy’s 5A medium (Gibco, #16600082) and Dulbecco’s Modified Eagle’s medium (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... Adult human dermal fibroblast (HDFa) cells (Cascade Biologics C-013-5C) were maintained in Human Fibroblast Expansion Basal Medium (Gibco) supplemented with Low Serum Growth Supplement (Gibco).
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bioRxiv - Bioengineering 2021Quote: ... and each lobe was cultured statically in a 6 well plate in cell culture media (DMEM high glucose, 10% FBS, 1% pen-strep) (Thermo Fisher Scientific, Waltham, MA). Media was changed on days 1 ...
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bioRxiv - Bioengineering 2021Quote: ... The sections were cultured statically in a 24 well plate and incubated for and additional hour before adding cell culture media (DMEM, 10% FBS, 1% pen-strep) (Thermo Fisher Scientific, Waltham, MA) for 5 days ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% fetal bovine serum (NTC-500, FBS; Natocor, Argentina) and 1% penicillin/streptomycin (ref. 15140122, Pen/Strep; Thermo Fisher Scientific, United States), while prostate cancer PC3 cells and breast cancer T47D cells were cultured in Roswell Park Memorial Institute medium (ref ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were finally washed with ChP epithelial cell (CPEC) medium (DMEM+10%FBS+1xPen/strep) and re-suspended in 1xHBSS + glucose + LIVE/DEAD staining kit (ThermoFisher, Catalog: L-3; 224). Live cells identified by green fluorescence (calcein-AM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% of exosome-depleted FBS (EXO-FBSHI-50A-1; SBI LLC., USA) and 1% of Pen-Strep-Glutamine (Gibco-Thermo Fisher Scientific Inc., USA) for 24 hours in a humidified incubator at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were quantified using a Pierce BCA Protein Assay Kit (ThermoFisher). Equivalent amounts of protein were resolved on precast polyacrylamide denaturing gels and were then transferred onto 0.2 μm PVDF membranes using either dry transfer with an iBLOT2 system (ThermoFisher ...