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Citations for Thermo Fisher :
6051 - 6100 of 10000+ citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... in PBS with 0.05% Tween-20 (PBST) and incubated in primary antibody and respective secondary antibody (Alexa Fluor; Thermo Fisher Scientific) in blocking buffer diluted 1:5 with PBST ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with anti-Sgs1 antibody (dilution 1:500) and anti-V5 antibody (dilution 1:1000, Thermo Fisher Scientific) at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were washed three times in PBST and incubated for one hour at room temperature with the fluorescent secondary antibody (Goat anti-mouse or Goat anti-rabbit antibodies conjugated with either Alexa488 or Alexa555, 1:400 dilution, Life technologies) in the same blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... The lysates were then resolved by SDS-PAGE and immunoblotted with indicated antibodies (Wapl and V5-tag antibodies were purchased from Thermo Fisher Scientific #PA5-38024 and #R960-25 ...
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bioRxiv - Neuroscience 2019Quote: ... brain sections were washed 3 times with PBS-T and incubated with appropriate fluorophore-conjugated or streptavidine-fluorophore conjugated (for APOE biotinylated antibody) secondary antibodies (1:500, Life Technologies) together with nuclear stain Hoechst 33342 (1:2000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-PrimPol antibody (Table 1) was coupled to magnetic M-270 Epoxy Dynabeads® (7.5 μg antibody/ mg beads; Life Technologies), and IPs were performed following manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Western blots were carried out using iBind with 1 ug/ml primary antibodies and 1 ug/ml secondary antibodies following iBind protocol (Invitrogen/ThermoFisher).
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were visualized using donkey anti-rabbit antibody conjugated with Alexa Fluor Plus 555 (1:1000, #A32794, Thermo Fisher Scientific) and donkey anti-goat antibody conjugated with Alexa Fluor Plus 488 (1:1000 ...
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bioRxiv - Systems Biology 2020Quote: ... Visualization of antibody signals was achieved using HRP-conjugated secondary antibodies and the chemiluminescence substrate SuperSignal West Pico (Thermo Fisher Scientific). Blots were imaged and quantitatively analyzed using a Chemidoc MP imager (Biorad) ...
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bioRxiv - Neuroscience 2022Quote: The primary antibodies were as follows: mouse anti-HA tag (2-2.2.14) antibody (1:1000; Thermo Fisher Scientific, cat. no. 26183); rabbit anti-HA tag (C29F4 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After 2 washes to remove any excess antibody cells were stained with anti-Rabbit Alexa-546 antibody (Life technologies, Cat#A11035). After 3 washes to remove any non-specific antibody nuclei were stained using Hoechst at 1:50,000 dilution and imaged on a Nikon Ti Eclipse automated microscope ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times with PBS and then incubated with secondary antibody (1:1000 goat anti-rabbit antibody or goat anti-mouse antibody conjugated to Texas Red and 1:1000 goat anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies) and 1:1000 donkey anti-goat antibody conjugate to Texas Red (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The secondary anti-mouse and anti-rabbit horseradish peroxidase-conjugated antibodies and the Alexa Fluor 488- and 555-congugated antibodies were from Thermo Fisher Scientific.
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bioRxiv - Biophysics 2020Quote: ... incubated with an anti-mouse antibody produced in goat and labeled with a goat anti-mouse antibody at 1:200 (Molecular Probes, tetramethylrhodamine ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibody staining was performed as per the primary with the appropriate AlexaFluor 488 conjugated secondary antibody (Life Technologies Ltd, UK) at a concentration of 1:50 ...
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bioRxiv - Developmental Biology 2020Quote: ... primary antibodies were detected using appropriate secondary antibodies coupled to Alexa-Fluor 488 and Alexa Fluor 594 (1:100; Molecular Probes) and diluted in PBTr for 2 days at 4°C.
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bioRxiv - Immunology 2019Quote: pOV8/Kb levels were determined by incubating cells for one h on ice with the 25-D1.16 antibody followed by one h on ice with secondary goat anti-mouse antibody conjugated to Alexa Fluor 647 (Molecular Probes). Cellular fluorescent proteins and Alexa Fluor 647 levels were determined on a FACS Calibur cytofluorograph (Beckton Dickinson ...
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bioRxiv - Pathology 2020Quote: ... The binding sites of the primary antibodies were revealed with Alexa Fluor-594 goat anti-rabbit and Alexa Fluor-488 goat anti-mouse secondary antibodies (Invitrogen, USA). Nuclei were stained with 4’ ...
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bioRxiv - Microbiology 2020Quote: ... The resulting blots were probed with a sdAb as primary antibody and an HRP-linked 6x-His Tag antibody (Thermofisher, HIS.H8) as the secondary antibody ...
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bioRxiv - Pathology 2020Quote: ... The secondary antibodies used to reveal LC3 and LAMP2 were Alexa 594-labeled anti-rabbit secondary antibody and Alexa 546-labeled anti-mouse secondary antibody (Invitrogen, USA), respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed thrice in PBS containing 0.05% Tween 20 and incubated with secondary antibody (anti-mouse antibody conjugated to Alexa 647 or Alexa 546, Thermo Fisher Scientific) at 1:4000 dilution and GFP nano-booster (Chromotek ...
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bioRxiv - Microbiology 2021Quote: ... or rabbit TgALD1 antibody (1:200, kind gift from Dr. Kentaro Kato) and anti-rabbit HRP antibodies (Thermo Fisher, 1:10000) followed by development of signal with West Pico Plus Chemiluminescent Substrate (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2021Quote: ... as the primary antibody at a 1:100 dilution and anti-mouse IgG Alexa Fluor 488 as the secondary antibody (A11029, Life Technologies) at a 1:100 dilution ...
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bioRxiv - Microbiology 2020Quote: ... and rabbit anti-SERA5 (1:500) (54) primary antibodies in combination with appropriate fluorophore-coupled secondary antibodies (1:1,000; Thermo Fisher Scientific). Stainings with Lysosensor Blue DND-167 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: The rabbit α-NOCT polyclonal antibody was produced in rabbits inoculated with MBP-NOCT (1-431) (Thermo Fisher custom antibody services). This MBP-NOCT antigen (1-431 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated in primary antibodies for 48 h at 4°C and secondary antibodies conjugated to AlexaFluor-488 or AlexaFluor-594 (Life Technologies) for 2 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were incubated with the antibodies indicated above and visualized with horseradish peroxidase–conjugated secondary antibodies using the ECL Western blotting system (Thermo Scientific).
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bioRxiv - Cell Biology 2019Quote: ... The membranes were washed for 15 min in TBST and then incubated for 1 h with HRP-conjugated secondary antibodies (1:5,000; Pierce Antibodies; Thermo Fisher Scientific) in TBST at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... either fluorophore-coupled secondary antibodies or HRP-conjugated secondary antibodies and WesternBright Chemiluminescent Substrate (Biozym Scientific) or SuperSignal West Pico Chemiluminescent Substrate (Life Technologies) were used ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated with the secondary antibodies (1:1000, Goat anti-Mouse IgG secondary antibody, Alexa Fluor 488, Life technologies, USA) for 2 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... Binding of the primary antibody was detected by incubation with Alexa Fluor 488-conjugated goat anti-mouse or secondary antibody (Invitrogen Molecular Probes). The sexual stages were highlighted by double-labelling with rabbit antibodies directed against Pfs230 ...
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bioRxiv - Cell Biology 2019Quote: ... Aortae were perfused in situ with PBS and then perfusion-fixed with 4% paraformaldehyde prior to staining using specific primary antibodies and AlexaFluor568-conjugated secondary antibodies (Life Technologies) or with AlexaFluor568-phalloidin (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... and secondary antibody solution was added (1-10ug/ml) (Alexa Fluor 488 Donkey anti-mouse IgG secondary antibody, ThermoFisher, A-21202). DAPI staining was used for nuclei ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were incubated in secondary antibodies (Alexa Fluor 488-, 555-, 594-, 647-conjugated secondary antibodies, or anti-mouse IgG isotype-specific secondary antibodies; 1:500; Life Technologies, Carlsbad ...
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bioRxiv - Immunology 2021Quote: ... and incubated with the secondary antibody (Alexa-Fluor 647- or Alexa-Fluor 405-conjugated goat anti-mouse Ig antibodies; Invitrogen, France) for 45 min at room temperature ...
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bioRxiv - Physiology 2020Quote: ... Secondary antibody at a 1/1000 dilution (Alexa Fluor 594 goat anti-rabbit antibody or Alexa Fluor 488 goat anti-mouse antibody, Thermo Fisher) in blocking buffer also containing Alexa Fluor-405 phalloidin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: GFP was visualized using rabbit polyclonal or mouse monoclonal anti-GFP primary antibodies and Alexa 647-labelled secondary antibodies (Molecular Probes/Invitrogen) directed against rabbit or mouse ...
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bioRxiv - Biochemistry 2021Quote: ... or a major outer membrane protein (MOMP) antibody (1:1000, Novus Bio) and an appropriate AlexaFluor secondary antibody (1:200, ThermoFisher Scientific). Counterstaining was performed with Hoechst stain ...
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bioRxiv - Neuroscience 2021Quote: ... 13) anti-Lectin antibody (#DL-1174, Vector, Burlingame, CA, USA) and anti-CD144 antibody (#14-1441-82 Invitrogen, Carlsbad, CA, USA) to label vascular and endothelia.
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bioRxiv - Neuroscience 2021Quote: ... an anti-P2X3 antibody (1:1000, Alomone APR016, anti-rabbit polyclonal) and an Alexa-488 conjugated secondary antibody (1:1000, Invitrogen A21206). The mean gray value of each DRG neuron was measured in ImageJ and a custom-made R toolkit (https://github.com/amapruns/Immunohistochemistry_Analysis ...
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bioRxiv - Cell Biology 2022Quote: ... Australia) primary antibody and a donkey anti-mouse IgG (H+L) secondary antibody conjugated to Alexa Fluor 568 (A10037; ThermoFisher, France), essentially as described (28) ...
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bioRxiv - Genomics 2020Quote: ... We used 4 ug of antibody to H3K27ac and captured the chromatin-antibody complex with 34 uL Protein A beads (Thermo Fisher). Qubit quantification following ChIP ranged from 125-150 ng ...
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bioRxiv - Microbiology 2021Quote: Viral N protein was detected using a rabbit anti-SARS-CoV N antibody [32] as a primary antibody with AlexaFluor 568 anti-rabbit IgG or anti-rabbit IgG-HRP (Thermo Fisher) as secondary antibodies together with DAPI to stain the nucleus by indirect immunofluorescence as described previously [33].
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bioRxiv - Microbiology 2020Quote: ... Fluorescently conjugated secondary monoclonal antibodies (Alexa Fluor 488 and 555 conjugated antibody series against mouse, rabbit, or goat from Life Technologies) were used for detection ...
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bioRxiv - Neuroscience 2021Quote: ... The secondary antibody used is goat anti-mouse IgG antibody conjugated with Alexa-568 (diluted to 2 μg/ml, A-11004, Molecular Probes).
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bioRxiv - Cancer Biology 2021Quote: ... Then coverslips were inverted onto 100 µL of antibody block with secondary antibodies (Alexa Fluor 488 anti-mouse - 1:200, Life Technologies #A11029 ...
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bioRxiv - Immunology 2020Quote: ... Primary antibody labeling was followed by labeling with anti-mouse-AlexaFluor-647 and anti-rabbit-AlexaFluor-555 secondary antibodies (Life Technologies). Flow cytometry data was collected using a FACSCanto II flow cytometer (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... and subjected to primary antibody and HRP conjugated secondary antibody before visualization with Pierce™ ECL Western Blotting Substrate (Thermo Fisher). Western blot images were obtained using the ChemiDoc Imager (BioRad).
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bioRxiv - Developmental Biology 2022Quote: ... The samples were incubated with primary antibodies overnight at 4°C and subsequently with fluorescence-conjugated secondary antibodies (Thermo Fisher Scientific) and DAPI for 1 hour at RT ...
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bioRxiv - Biochemistry 2022Quote: ... or monoclonal mouse antibodies directed against TBEV E [21] and conjugated secondary antibodies anti-rabbit Alexa555 (1:500, Thermo Fisher Scientific). Nuclei were counterstained with DAPI ...