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Citations for Thermo Fisher :
5951 - 6000 of 10000+ citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Stealth RNAiTM siRNAs Negative Control, Med GC and human Il11, NM_000641.3 (HSS179893, HSS179894, HSS179895) were purchased from Thermo Fisher Scientific.
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bioRxiv - Biochemistry 2021Quote: DNA sequence coding for human Xkr8 (hXkr8) (UniProt: Q9H6D3) was custom-designed and synthesized by GeneArt (Thermo Fisher Scientific) to enhance mRNA stability and translational efficiency ...
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bioRxiv - Bioengineering 2021Quote: ... and resuspended in B8 media with varying concentrations if iMatrix laminin and/or truncated recombinant human vitronectin (ThermoFisher #A14700). Cells were seeded at 5,000 cells/cm2 ...
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bioRxiv - Bioengineering 2020Quote: ... and MDA-MB-231 (ATCC HTB-26) human breast cancer cells were cultured in medium composed from standard Dulbecco’s Modified Eagle Medium (DMEM, Gibco), 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2021Quote: ... Human ACE2 ectodomain (NCBI Reference Sequence: NP_001358344.1, residues Q18-S740) was expressed in Expi293™ cells (Thermo Fisher Scientific) with a preceding murine IgG1 signal peptide ...
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bioRxiv - Biochemistry 2021Quote: ... Wild type/native human basigin (BSG-2, residues M1-L206) was expressed in Expi293™ cells (Thermo Fisher Scientific) with C-terminal rat CD4 domains 3+4 (CD4d3+4 ...
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human Alexa-647 [Cat# A21445] and streptavidin-AlexaFluor 635 [Cat# SA1011] antibodies were obtained from Thermo Fisher. Control lectins Erythrina cristagalli agglutinin (ECA ...
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bioRxiv - Neuroscience 2021Quote: Human neuroglioma cells (H4) were maintained in Opti-MEM I Reduced Serum Medium (Life Technologies- Gibco, Carlsbad, CA, USA) and Human Embryonic Kidney 293 (HEK ...
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bioRxiv - Neuroscience 2021Quote: Human neuroglioma cells (H4) were maintained in Opti-MEM I Reduced Serum Medium (Life Technologies- Gibco, Carlsbad, CA, USA) and Human Embryonic Kidney 293 (HEK ...
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bioRxiv - Immunology 2021Quote: ... Human foreskin fibroblasts (HS27, ATCC CRL-1634) were hold in culture in Iscove’s Modified Dulbecco’s Medium (IMDM, Invitrogen/Gibco) with the same supplementations ...
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bioRxiv - Neuroscience 2020Quote: 100 ng of total RNA was subjected to hybridization on the Human Clarion S Array (Affymetrix, Thermo Fisher Scientific) at the BMFZ (Biomedizinisches Forschungszentrum ...
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bioRxiv - Neuroscience 2020Quote: 100 ng of total RNA was subjected to hybridization on the Human Clarion S Array (Affymetrix, Thermo Fisher Scientific) at the BMFZ (Biomedizinisches Forschungszentrum ...
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bioRxiv - Cell Biology 2021Quote: Young adult primary human skeletal muscle (HSKM) progenitors isolated from a 20-year old female subject’s quadriceps muscles (Gibco) were seeded onto plates coated with 0.1% gelatin solution (Merck-Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: Human iPS cells (iPSCs, ATCC-DYS0100) were maintained in self renewal on a layer of Geltrex (Gibco, A14133-01), in Essential 8 Basal Medium (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... We used summarized log-scale intensities representing copy number profiles generated by combining probe intensities from four platforms (Agilent Human Genome CGH Microarray 44A, Nimblegen HG19 CGH 385K WG Tiling v2.0, Affymetrix GeneChip Human Mapping 500k Array Set and Illumina Human1Mv1_C BeadChip) ...
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bioRxiv - Cancer Biology 2019Quote: ... The HER4 JMa/CYT1 human sequence was amplified by PCR and subcloned in the pcDNA3.1 vector (Invitrogen, Carlsbad, CA) to create pCMV_HER4 ...
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bioRxiv - Neuroscience 2019Quote: Silencing lentiviral vectors were produced by co-transfecting HEK293T producing cells with lentiviral silencing plasmids GIPZ Human histamine H3 receptor shRNA (Clone V3LHS_638095 or Clone V3LHS_638091, Thermo Scientific) with packing plasmid psPAX2 and envelope coding plasmid pMD2.G (Addgene#12260 and #12259 ...
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bioRxiv - Biochemistry 2021Quote: ... Human telomerase reverse transcriptase immortalized retinal pigment epithelial cells (hTERT-RPE1, ATCC CRL-4000) were cultured in DMEM:F12 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2020Quote: ... The microarray slides were probed with Fc-tagged DC-SIGN and L-SIGN at 10 μg/ml diluted in 1% BSA (TSM with 0.05% Tween) and binding was detected with Alexa Fluor 488 labelled goat anti-human IgG (H+L) (Invitrogen) at 5 μg/ml.
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bioRxiv - Biochemistry 2020Quote: ... and the HsGlud2 cDNA clone (NM_012084.3) was obtained from the Ultimate™ ORF Lite human cDNA collection (Life Technologies). Amino acid mutations were introduced via Quick Change site-directed mutagenesis as previously described(58) ...
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bioRxiv - Bioengineering 2021Quote: ... Secreted cytokine analysis was then performed using a ProcartaPlex essential Th1/Th2 human cytokine immunoplex assay according to manufacturer’s instructions (Invitrogen). Immunoassay was then read on a Luminex 200 instrument controlled by BioPlex manager software (version 6.2).
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma cells (HCT-8 cells, ATCC CCL-244) were maintained in RPMI 1640 medium (Gibco, ATCC modification) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... USA) and Huh7.5 (human liver cell line) (kind gift from Dr. Craig Cameron) were cultured in DMEM (Life Technologies) containing 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Genetics 2021Quote: ... A database search was performed using the human SwisProt sequence and Orf8 sequence with Proteome Discoverer 2.4 (Thermo Scientific) with the following search criteria ...
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bioRxiv - Genetics 2021Quote: ... A database search was performed using the human SwisProt sequence and Orf8 sequence with Proteome Discoverer 2.3 (Thermo Scientific) with the following search criteria ...
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bioRxiv - Physiology 2020Quote: ... MCF-10 human mammary epithelial cells and COS-7 fibroblast-like monkey cells were maintained in DMEM (Fisher Scientific) supplemented with 10% fetal bovine serum and 2% penicillin/streptomycin.
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Microbiology 2021Quote: ... A549 (male, human lung epithelial-like) cells were obtained from ATCC and grown at 37 °C in DMEM (Gibco) supplemented with 10 % FBS (GE Healthcare Life Science ...
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bioRxiv - Systems Biology 2020Quote: ... and then cells were fixed and permeated in the plate using a Human Cardiomyocyte Immunocytochemistry Kit (Thermo Fisher Scientific). The primary antibodies included ...
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bioRxiv - Microbiology 2020Quote: ... 100 µl of TSB containing 10 µg/ml goat anti-Human IgG conjugated to Alexa fluor 647 (A21445, Invitrogen) and 3%(v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... and Caco-2 cells (human, male, colon; HTB-37, ATCC, RRID: CVCL_0025) were cultured in minimum essential medium (GIBCO) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... GAPDH expression was used as an internal control to normalize input cDNA (human GAPDH: Assay ID, Hs02758991_g1, Applied Biosystems). Ratios of the expression level of each gene to that of the reference gene were then calculated.
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bioRxiv - Cancer Biology 2020Quote: RNA from frozen tumor bulk tissues was used for Affymetrix gene expression array (Affymetrix Human Genome U133 Plus 2.0) and DNA in Infinium Methylation EPIC kit (Illumina).
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bioRxiv - Cell Biology 2021Quote: ... mouse embryonic fibroblast and human fibroblasts were maintained in Eagle’s Dulbecco’s Modified Eagle’s Medium (DMEM; Life Technologies Inc., USA) supplemented with 10% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... PCoV_GX and RaTG13 pseudoviruses were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective spike protein into 293T cells (ATCC) ...
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bioRxiv - Cancer Biology 2021Quote: ... The 384-well plate was loaded on the the TaqMan Open Array Human MicroRNA Panel plates (Thermo Fisher, USA) and analyzed using QuantStudio™ 12K Flex (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA of RBMX were obtained from the human ORFeome collection (V5.1) and was cloned into DLAP and DHA destination vectors using gateway cloning technology (Invitrogen) to make LAP-RBMX and HA-RBMX ...
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bioRxiv - Cell Biology 2020Quote: ... and U-2 OS (female, human osteosarcoma epithelial) cells from ATCC were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; Life Technologies) supplemented with 10% v/v fetal bovine serum ...
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bioRxiv - Cell Biology 2022Quote: Immortalized human endometriotic epithelial cells (12Z cells) (16) were cultured using Dulbecco’s modified Eagle’s medium (DMEM)/F-12 (Gibco, USA) supplemented with 10% Charcoal-dextran treated fetal bovine serum (CDS-FBS ...
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bioRxiv - Neuroscience 2022Quote: The total amount of soluble tau in S1 fractions was determined by using an ELISA kit (human Tau, Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... and human T lymphocytes, Jurkat J16 (Adult lymphoblastic leukemia ACC-282, DSMZ) were cultured in RPMI-1640 (Thermo Fisher) supplemented with 10% FCS ...
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bioRxiv - Microbiology 2022Quote: ... and human foreskin fibroblast cells immortalized with human telomerase reverse transcriptase (HFF-hTERT cells)96 were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... falciparum erythrocytic stages were cultured in human erythrocytes (National Blood Transfusion Service, UK) and RPMI 1640 medium (Life Technologies) supplemented with 0.5% Albumax type II (Gibco) ...
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bioRxiv - Synthetic Biology 2022Quote: Codon-optimized DNAs encoding the variable library parts were codon optimized for expression in human cells using ThermoFisher GeneArt’s website tool and synthesized by ThermoFisher GeneArt ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... mice were bled and total IgG (mouse and human) was pulled down using protein A beads (ThermoFisher, Catalog # 20333). An in–house sandwich ELISA was used to measure human IgG (control IgG or MS-Hu6 ...
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bioRxiv - Biochemistry 2022Quote: Human Embryonic Kidney Expi293 cells were grown to a density of approximately 2×106 in Expi293 medium (Thermo Scientific). Following stress induction upon addition of 100 mM (final concentration ...
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bioRxiv - Bioengineering 2022Quote: cDNA libraries were constructed using Ion Ampliseq Transcriptome Human Gene Expression Kit from Thermo Fisher (MA, USA, Cat# A26325) according to manufacturer’s recommended protocol ...
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bioRxiv - Bioengineering 2022Quote: ... Rat ASC were grown in the same medium than human ASC supplemented with 10% of fetal calf serum (Thermofisher). To manufacture the ASC-enriched gelatin patch ...
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bioRxiv - Cell Biology 2022Quote: Human mammary epithelial MCF-10A cells were cultured in basal Dulbecco’s Modified Eagle Medium (DMEM) (Gibco by Life Technologies) with 10% Fetal Bovine Serum (FBS ...