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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cytoplasm lysates were precleared with protein G magnetic beads (Invitrogen) and immunoprecipitated using anti-Rb cGAS (CST ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were first quantified with a BCA assay (ThermoFisher Scientific). A total of 30 µg of total protein from each sample was saved as input material ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysates were applied to HisPur cobalt affinity resin (ThermoFisher), washed with Buffer A containing 500 mM NaCl and 5 mM imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... the lysate was transferred into Phasemaker tubes (Thermo Fisher Scientific), and the phase separation was performed in a single step according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were sonicated via a Sonic Dismembrator 100 (Fisher Scientific) at setting 3 with 3-4 pulses of 15 s on/off on ice and incubated for an additional 1 hr at 4 °C with end-to-end rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... from the cellular lysates using a fluorometric assay (Qubit, Invitrogen). Based on the assumption that an E12.5 embryo contains approximately 6 million cells3 ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were mixed with 4x NuPage LDS sample buffer (ThermoFisher). Gels were loaded to NuPage 4-12% Bis-Tris precast gels (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: Lysates were run on 4-12% polyacrylamide gels (NuPage, Invitrogen) for 50 minutes at 180V and transferred to polyvinylidene difluoride (Immobilon-FL ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were homogenized using a microtip probe sonicator (Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Lysates were homogenized using a microtip probe sonicator (Fisher Scientific) and clarified by centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were run on 4-12% Bis-Tris gel (Invitrogen), transferred to PVDF ...
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bioRxiv - Cell Biology 2023Quote: ... Normalized lysates were mixed with sample-loading buffer (Invitrogen NP0007) containing 2-mercaptoethanol (80μL added to 920μL sample-loading buffer ...
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bioRxiv - Cancer Biology 2023Quote: Whole cell lysates were prepared in SDS Lysis Buffer (ThermoFisher) and resolved on 3-8% or 4-12% gradient SDS-PAGE gels (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Protein lysates were analyzed on 4–12% NuPAGE gels (Invitrogen), transferred to PVDF membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were treated with Turbo DNase (Thermo Fisher Scientific) for 10 min at 4°C and then clarified by removal of cellular debris by centrifugation at 20,000[×[g for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell lysates were made in SDS Lysis Buffer (ThermoFisher) and resolved on 3-8% or 4-12% gradient SDS-PAGE gels (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... These lysates were prepared by a sample buffer (NP0007; Invitrogen) with 10 % 2-Mercaptoethanol and loaded onto 4-20% precast polyacrylamide gels (4561096 ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were incubated with NuPAGE 4× LDS buffer (Invitrogen NP0008) with 50 mM dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate was loaded onto a Ni-NTA column (Thermo Fisher) and protein was eluted using 300mM Imidazole ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were precleared with Dynabeads™ Protein A (Invitrogen) for 1h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were then treated with RNase I (Thermofisher, cat. #EN0602), first diluted to the indicated concentration by volume (e.g. ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein lysate concentration was determined by BCA assay (Thermo Scientific) and 30 μg of each lysate was fractionated by SDS–PAGE on a 4-12% polyacrylamide gel and transferred onto PDVF membrane (Amersham) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was purified from the lysate using TRIzo (ThermoFisher, 15596018) and quantified on NanoDrop ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were pre-cleared using dynabeads (Cat# 10003D, Invitrogen) conjugated with normal mouse IgG (Cat# 12-371 ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were prepared in RIPA lysis buffer (ThermoFisher Scientific) supplemented with a protease inhibitor cocktail (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were pre-cleared with Dynabeads Protein G (ThermoFisher, 10004D) conjugated to Normal Mouse IgG (Table 1) ...
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bioRxiv - Neuroscience 2024Quote: ... Lysate was then mixed with UltraPure phenol:chloroform:isoamyl alcohol (ThermoFisher, #15593049) in a 5:1 ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole cell lysates were prepared in RIPA buffer (Thermo Fisher) supplemented with tablets of cOmpleteTM protease inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: Whole-cell lysates were processed using RIPA buffer (Thermo Fisher) supplemented with protease inhibitors ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were treated with RNase I (Ambion, 100 U/µL) at room temperature for 45 minutes in slow agitation ...
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bioRxiv - Microbiology 2020Quote: Concentrated virus samples were mixed with Novex™ Tris-Glycine SDS Sample Buffer (2X) (Thermo Fisher Scientific) with NuPAGE™ Sample Reducing Agent (10X ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with virus containing scrambled control or targeting MTR and Polybrene (8 ug/mL, Invitrogen). Cells were split after 48 h into standard RPMI-media (10% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion, Life Technologies, Carlsbad, CA) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion, Life Technologies, Carlsbad, CA) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... the virus solution was typically mixed at 1:500 with blue beads (fluorescent polymer microspheres, Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... Reovirus S4 RNA was quantified using the Taq-Man Fast Virus One-Step Master Mix (Applied Biosystems) and GAPDH was detected as an endogenous control using the Pre-Developed TaqMan Assay Reagent kit for mouse GAPDH (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... reverse transcription was performed with random hexamers and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen). Synthesized RNA was used as a standard (BEI) ...
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bioRxiv - Biochemistry 2021Quote: ... high titer virus generation (V1) was then used to infect 1200 ml High Five™ cells (Invitrogen) for large scale protein production ...
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bioRxiv - Microbiology 2020Quote: ... centrifuged and suspended in virus media that is composed of DMEM high glucose (Thermo Fisher Scientific, USA), with 2% fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Master mix for qRT-PCR was prepared using TaqMan Fast Virus 1-step Master Mix (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from 1010 virus particles using the PureLink TM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were generated by Sendai virus reprogramming and clonal expansion using the CytoTune – iPSC 2.0 kit (ThermoFisher) to deliver the reprogramming factors ...
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Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: Each RNA sample was subjected to reverse transcription using murine leukemia virus reverse transcriptase (Thermo Fisher Scientific), and the generated cDNA was used as a template for qRT-PCR ...
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bioRxiv - Immunology 2021Quote: ... Reactions were performed using TaqMan® Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Waltham, USA) and 5 μl of isolated RNA as a template ...
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bioRxiv - Microbiology 2020Quote: ... Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) and a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Microbiology 2021Quote: Virus stock were diluted in 10-fold serial dilutions in 2ml Opti-MEM I (1X) + GlutaMAX (Gibco). 1 ml of each dilution was added to monolayers of 2 × 106 VeroE6 cells in the same medium in a 6-well plate ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNAs were synthesized using Moloney murine leukemia virus RT with random primers (Invitrogen, Thermo Fisher Sci.). RNA levels of representative transcripts were analyzed in triplicate by quantitative PCR with SYBR™ Green using a Bio-Rad CFX thermal cycler ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNAs were synthesized using Moloney murine leukemia virus RT with random primers (Invitrogen, Thermo Fisher Sci.). RNA levels of representative transcripts were analyzed in triplicate by quantitative PCR with SYBR™ Green using a Bio-Rad CFX thermal cycler ...
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bioRxiv - Immunology 2020Quote: ... Virus stocks used in vivo were produced by two passages on Vero E6 cells in DMEM (Gibco) without FBS ...
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bioRxiv - Microbiology 2021Quote: ... Wild-type mumps virus (MuVi/Utrecht.NLD/40.10; genotype G) was grown on Vero cells in DMEM (Gibco) with 2% FBS ...