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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with 10-fold serial dilutions of the virus for 1 h and overlaid with 0.55% methylcellulose or 1.0% cellulose in minimal essential medium (MEM) (Gibco) supplemented with 2% FBS (Corning ...
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bioRxiv - Genomics 2024Quote: ... Virus was produced by transfecting HEK293T cells with the plasmid and the Lipofectamine 3000 kit (Thermo Fisher Scientific). U87MG were transduced with the supernatant containing the NY-ESO-1 virus ...
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bioRxiv - Microbiology 2024Quote: ... and CC81 (feline cell line transformed by mouse sarcoma virus ECACC 90031403) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-high glucose (GlutaMAX, Gibco) supplemented with 1% penicillin/streptomycin and 10% fetal bovine serum (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... Virus production medium contained DMEM supplemented with L-glutamine and sodium pyruvate and 10% heat-inactivated FBS (Gibco). Viral supernatants collected roughly 48- and 72-hours post-transfection were sterile-filtered through a 0.45-micron filter and concentrated in 100kDa molecular weight cutoff filters.
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin lysates were incubated with 10μg antibody-bound Dynabeads (Dynabeads: Fisher Scientific #14-311-D ...
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bioRxiv - Cell Biology 2020Quote: ... Protein content of the lysate was determined by BCA assay (Thermo Fisher). For analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Protein lysate content was quantified by micro BCA assay (Thermo-Fisher Scientific) and 40 µg of protein were resolved by SDS-PAGE ...
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bioRxiv - Genetics 2021Quote: ... Lysates were quantified using the bicinchoninic acid assay (BCA, Thermo Fisher Scientific). For each sample ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were separated by 3%–8% Tris-Acetate SDS-Page (Thermo fisher) and probed against rabbit IP3R1 (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos lysates were loaded on precast gradient gels (4-12 %, NuPAGE, Invitrogen). Gels were transferred to a nitrocellulose membrane for western blotting and incubated with the indicated antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... cell lysates were loaded on a 4 - 20% Bis-Tris gel (Invitrogen) and proteins blotted onto a 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... the lysates were treated with Turbo DNase (Fermentas) and RNase I (Ambion). Protein G Dynabeads (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The lysate was then incubated with Anti-HA Magnetic Beads (Thermo Fisher) for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were mixed with Novex™ Tris-Glycine Native Sample Buffer (Invitrogen) and resolved using Novex™ 4-12% Tris-Glycine gels (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: Protein lysates were prepared in RIPA buffer and BCA Assay (Thermo Scientific) was used to determine protein concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... the lysates were incubated with 10 μL of Dynabeads Protein G (Invitrogen) for 1 hr at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate protein concentration was measured with BCA Protein Assay (Thermo Fisher 23252). Lysate was diluted to 4 mg/ml in Sup6-150 ...
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bioRxiv - Molecular Biology 2021Quote: Oocyte lysates or immunoprecipitates were resolved on 4-12% NuPAGE gels (Invitrogen) and transferred to polyvinylidene fluoride membranes (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... Crude lysate was used for reverse transcription with SuperScript III (Thermo Scientific). Equal fraction of total RNA per oocyte/zygote was reverse transcribed by SuperScript III RT (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Lysates and IP eluates were separated on 4-12% gradient gels (Invitrogen) and blotted on nitrocellulose membranes (Amersham ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates were resolved by NuPAGE 4-12% Bis-Tris gels (Invitrogen), and transferred onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 μg of cell lysates (in Pierce IP lysis buffer; #87787, ThermoFisher) derived from fully differentiated CAD or N2a cells was then incubated with 3′-desthiobiotinylated-RNA/streptavidin beads at 4°C for 1 hr under agitation (600 RPM) ...
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bioRxiv - Molecular Biology 2021Quote: Whole cell lysates were prepared using RIPA Lysis Buffer (Thermo scientific #89900) supplemented with a protease inhibitor cocktail (Roche# 11836170001 ...
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bioRxiv - Biochemistry 2019Quote: ... The cleared lysate was loaded onto HisPur Ni-NTA resin (Thermo Scientific), washed with lysis buffer and eluted with lysis buffer containing 250 mM imidazole ...
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bioRxiv - Physiology 2020Quote: ... Lysates were measured using the Pierce BCA assay kit (Thermo Scientific #23225) and normalized to 10 μg total protein ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein lysate samples were electrophoresed through 4-12% Bis-Tris gels (Invitrogen). Following electrophoresis ...
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bioRxiv - Biochemistry 2019Quote: ... Clarified lysate was applied to HisPur ™ Ni-NTA resin (Thermo Scientific) and the resin was washed extensively with buffer containing 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were precleared using 40.0 μl of protein G Dynabeads (ThermoFisher Scientific) for 1 hour prior to an overnight incubation at 4°C with either J2 ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were incubated with 200μl of GST-Sepharose beads (Thermo Scientific) and washed 6 times with CHAPS buffer containing 0.5 M NaCl and 3 times with CHAPS buffer containing 5 mM MgCl2 ...
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bioRxiv - Neuroscience 2019Quote: ... The fly lysate protein levels were determined by BCA assay (Thermo Fisher) and metabolite levels were normalized to protein level.
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bioRxiv - Cell Biology 2019Quote: Lysates were run on 4-12% Bis-Tris protein gels (Invitrogen #NP0335) and blots were probed with anti-GFP (Abcam #ab290 ...
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bioRxiv - Immunology 2019Quote: ... The lysate/antibody mixture was then incubated with Protein G Dynabeads (Invitrogen) for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was incubated with DNase I (10 μg/ml, Thermo Scientific) and RNAse A (10μg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were loaded onto NuPAGE 4-12% Bis-Tris Protein Gels (Invitrogen) and transferred onto Nitrocellulose membrane (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were diluted in NuPAGE™ LDS 4x sample buffer (ThermoFisher Scientific) plus NuPAGE™ 10x sample reducing agent (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... lysates were digested with 0.005 U/μg RNase I (ThermoFisher Scientific AM2295). Prior to library construction ...
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bioRxiv - Bioengineering 2020Quote: ... Protein lysates (20 μg) were separated on NuPAGE Tris-Acetate Gels (Invitrogen) and transferred onto PVDF membranes ...
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bioRxiv - Bioengineering 2020Quote: ... Cell lysates (20 ug) were diluted in 4x LDS buffer (Life Technologies) with 5% Beta-mercaptoethanol ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were resolved on SDS-PAGE gels (Novex Tris-Glycine, ThermoFisher Scientific) and transferred to PVDF membranes using the XCell II wet blotting system (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... The filtered lysate was then added to GST-beads (Thermo Fisher Scientific) pre-equilibrated in binding buffer and incubated for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... the cell lysate was centrifuged in a T-647.5 rotor (Thermo Fisher) at 40,000 rpm for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... the lysate separated on a 4-12% Bis-Tris gradient gel (Invitrogen) along with a protein ladder (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Cell lysates were prepared in 1x NuPAGE LDS sample buffer (Invitrogen, NP0008) containing 2-mercaptoethanol (final concentration 1.5% ...
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bioRxiv - Cell Biology 2021Quote: ... the lysate was incubated with Ni-NTA agarose resin for binding (Invitrogen), and later the resin was transferred to a Poly-Prep chromatography column (BioRad ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were mixed with NuPAGE LDS Sample Buffer (4X, Thermo Fisher Scientific) containing 50 mM DTT and heated for 5 min at 95°C ...
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bioRxiv - Immunology 2020Quote: ... Lysates were loaded run on Bis-Tris 4-12% Protein Gels (Invitrogen) then transferred to a PVDF membrane (Invitrogen ...
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bioRxiv - Pathology 2021Quote: ... cell lysate was incubated with Dynabeads® M-270 Epoxy (Life technologies) for 1 hour at 4°C under gentle rotation ...
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bioRxiv - Pathology 2021Quote: ... Neuronal lysates were loaded onto NuPage 12% Bis-Tris gels (Life technologies), separated by electrophoresis in MES buffer and transferred onto Protran nitrocellulose membranes for immunoblotting ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were resolved using NuPAGE 4-12% bis-tris gels (Life Technologies) and proteins transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2021Quote: ... WCL and EV lysates were quantified by BCA assay (Thermo Fisher 23225), mixed with sample buffer ...