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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... 40-50μg of protein was analysed by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using 3-8% NuPAGE Tris-Acetate protein gels (Life Technologies) and transferred onto nitrocellulose membrane using Xcell Sure Lock Mini Cell electrophoresis tanks (Novex ...
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bioRxiv - Genomics 2020Quote: ... An estimated 60 µg of protein was loaded per well on a NuPAGE Novex 3-8 % Tris-Acetate Protein gel (Life Technologies), separated by electrophoresis and blotted on polyvinylidene fluoride membranes ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Genetics 2021Quote: ... RNA was extracted from control and CRISPR-Cas9 targeted Calu-3 cells (N = 3 biological replicates, with 3 technical replicates per experiment per condition) and prepared using Trizol Reagent (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins in solution were cross-linked by 3 mM DSP (Thermo Fisher Scientific Pierce) and incubated during 2 h ...
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bioRxiv - Immunology 2020Quote: ... 50ug of total protein was loaded on 3-12% NativePAGE gel (#BN1001, Thermo Scientific). Gels were stained by Colloidal Blue Staining Kit (#LC6025 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions were loaded on 3-12% gradient Bis-Tris NativePAGETM precast protein gels (Invitrogen) and subjected to CN-PAGE electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein extracts were directly resolved on Tris-acetate 3-8% polyacrylamide NuPAGE gels (Invitrogen). Proteins were transferred to a nitrocellulose Hybond-C membrane (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... NuPAGE™ 3-8% Tris-Acetate Protein Gels was purchased from Invitrogen (Cat# EA03785BOX). Novex™ HiMark™ Pre-stained Protein Standard was purchased from Invitrogen (Cat# LC5699).
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bioRxiv - Molecular Biology 2023Quote: ... Samples were resolved in 3-8 % NuPage Norvex tris-acetate Protein Gels (Life Technologies) and transferred to PVDF membranes (Life Technologies) ...
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bioRxiv - Systems Biology 2023Quote: ... We then separated protein complexes using 3-8% Tris-acetate gels (Thermo Fisher, EA0378) and native running buffer (90 mM Tris Base ...
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bioRxiv - Biochemistry 2023Quote: Protein A/G agarose beads for immunpreciptation (Figure 3) were purchased from Thermo Fisher Scientific ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: Eluted proteins were separated on 3%–8% Tris-Acetate NuPAGE precast gels (Life Technologies) at 150 V for 70 min and were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2019Quote: ... Initial virus was recovered from T7 transcribed RNA using reverse transfection of Huh7.5.1 cells by Lipofectamine® 2000 (Invitrogen) (for virus rescue ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were generated by transfecting the clone into Vero cells using Lipofectamine 2000 (Thermo Fisher, cat# 11668-019), and harvested at various times post-transfection.
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bioRxiv - Biophysics 2021Quote: ... the protein was expressed as a fusion construct with an N-terminal His6 tag followed by tobacco etch virus (TEV) protease cleavage site in E.coli BL21 DE3 Star cells (Invitrogen). The fusion construct was purified using immobilized-nickel affinity chromatography ...
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bioRxiv - Microbiology 2022Quote: ... Viral stocks were passaged and expanded using MDCK-II cells in virus growth medium comprised of Opti-MEM (Gibco), 2.5 mg/mL bovine serum albumin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Trypsin used for virus propagation was standard cell culture grade 0.25% porcine trypsin with EDTA and phenol red (ThermoFisher). Huh-7 cells were seeded on a 24-well plate and incubated at 37 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Viral plaques were passaged at an MOI of ∼0.001 in MDCK-II cells in virus growth medium comprised of Opti-MEM (Gibco), 2.5 mg/ml bovine serum albumin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was removed after 24 h and cells were cultured in fibroblast medium with 10% FBS (Gibco, 16000-044) in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from the supernatant of MDCK cells infected with the PB2 TRAP V5 virus using Trizol (Invitrogen). RNA was reverse-transcribed with Superscript III (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... MRC-University of Glasgow Centre for Virus Research) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher, 10566016) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... were infected with HSV-1 at MOI 0.01 by replacing cell media with virus diluted in minimal media Opti-MEM (ThermoFisher). The virus was allowed to adhere to cells at 37 °C in 5% CO2 for 1 hour with minimal agitation every 15 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Cell surface proteins were biotinylated and isolated using Pierce Cell Surface Protein Isolation Kit (#89881, Thermo Fisher). All the steps were performed at 4°C unless otherwise mentioned ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell counting was performed using a Countess 3 Automated Cell Counter (ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... Cell counting was performed with a Countess 3 Automated Cell Counter (ThermoFisher).
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bioRxiv - Cancer Biology 2023Quote: Cells were trypsinized and counted using Countess 3 cell counting system (Invitrogen) and 2×103 cells/per well were plated in a 96 well plate for SW620 and WiDr cells (1×103 cells were plated for Colo320HSR cells) ...
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bioRxiv - Neuroscience 2023Quote: ... The cell number was counted by the cell counter (Countess 3, ThermoFisher). The cells were then centrifugated for 5 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... After counting cells in Countess 3 Automated Cell Counter (Thermo Fisher Scientific), two independent 10x genomics ports were loaded with either 16k Tomato+ cells (90% viability ...
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bioRxiv - Physiology 2023Quote: Cell surface proteins from transfected HEK293 cells were biotinylated using the Pierce Cell Surface Protein Isolation Kit (ThermoFisher Scientific., cat# 89881). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... sonicated 3 three times for 5 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 μl (1 μg/μl ...
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bioRxiv - Cancer Biology 2021Quote: ... A total amount of 30 µg protein was loaded per lane of NuPAGE Tris-Acetate Protein Gel 3-8% (Life Technologies, EA0375BOX) and separated according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Challenged A549 cells were lysed with modified RIPA buffer (Supplementary Data 3) and protein concentration was determined using a bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific). Phospho-p38 (p-p38) ...
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bioRxiv - Immunology 2020Quote: ... sonicated 3 three times for 10 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 µg/µl of cell lysate proteins per lane were resolved on SDS-PAGE (10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... The 3 eluted fractions were pooled and the protein concentrations measured with the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, 23225) using the Pierce™ Bovine Gamma Globulin Standard (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Pre-cleared lysates were further incubated at 4°C overnight with the indicated antibodies (1 to 3 μl) and protein A agarose or protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitates were washed three times with RIPA buffer (LSB ...
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bioRxiv - Microbiology 2019Quote: ... with TaqMan fast virus 1-step master mix (Thermo scientific) using AriaMx instrumentation (Agilent).
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bioRxiv - Cancer Biology 2019Quote: ... Virus was then resuspended in 1:1 Opti-MEM (Gibco) - HBSS ...
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bioRxiv - Microbiology 2019Quote: ... fibroblast-derived virus stocks were treated with TurboDNase (Thermo Fisher). For this ...
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bioRxiv - Immunology 2021Quote: ... Pelleted virus was resuspended in Opti-MEM (Gibco 31985-070) and stored at −80 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The virus pellet was resuspended in 1 mL PBS (Gibco). For long term storage ...
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bioRxiv - Cell Biology 2021Quote: ... In brief the virus was diluted in DMEM (ThermoFisher Scientific) to achieve a multiplicity of infection (MOI ...
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bioRxiv - Pathology 2022Quote: ... 7μl of 4x Taqman Fast Virus Master Mix (Thermo Fisher), 5μl of DNAse/RNAse free water and 3μl of primers and probe mix were used ...
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bioRxiv - Molecular Biology 2020Quote: ... and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) with 250 μM deoxy nucleoside triphosphate (dNTP ...
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bioRxiv - Microbiology 2019Quote: ... and Moloney murine leukemia virus reverse-transcriptase (MMLV-RT, Invitrogen). Subsequent PCR amplification performed in a thermocycler using specific primers ...
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bioRxiv - Genetics 2020Quote: Fibroblasts were infected by Sendai virus(Thermo Fisher Scientific, A16517) Transfected fibroblasts (approximately 1*105 cells per nucleofection ...
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bioRxiv - Microbiology 2021Quote: ... 7μl of 4x Taqman Fast Virus Master Mix (Thermo Fisher), 5μl of DNAse/RNAse free water and 3μl of primers and probe mix (sequences shown in Table 1) ...
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bioRxiv - Immunology 2020Quote: ... the virus inoculum was replaced with fresh medium (MEM, Gibco) containing 1.25% Avicel® (FMC Biopolymer ...
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bioRxiv - Immunology 2021Quote: ... Virus was diluted in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) and maintained on ice prior to infection ...
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bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...