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Citations for Thermo Fisher :
5901 - 5950 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The concentration of total protein was quantified by bicinchoninic acid (BCA) protein assay kit (ThermoFisher, #23228). Equal amounts of total protein were mixed with 4x loading dye and loaded onto a 12.5% SDS–PAGE gel and separated ...
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bioRxiv - Cell Biology 2019Quote: ... The collected supernatants’ protein concentrations were then determined by Pierce BCA protein assay kit (Thermo Scientific). An equal fraction of non-reducing SDS solution was then added to the proteins and the contents were boiled on a dry-block for 5 minutes ...
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bioRxiv - Immunology 2020Quote: ... Protein concentrations were determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific 23552). Samples were then prepared by addition of Nu PAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatant was collected and protein concentration was determined by BCA protein assay (Thermo Fisher Scientific). The protein suspension was separated by gel electrophoresis followed by transfer to nitrocellulose membranes ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... The final protein concentration was measured using a Pierce BCA protein assay kit (Thermo Fisher Scientific). An aliquot of ∼50 µg cell lysate was reduced with freshly dissolved 10 mM DTT for 30 min at 37 °C and alkylated with freshly dissolved iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Luminescence was normalized to protein levels determined by a Pierce BCA protein determination kit (Thermo Scientific).
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bioRxiv - Plant Biology 2021Quote: ... The concentration of proteins was measured by BCA protein assay (Pierce, Thermo Fisher Scientific, San Jose). A total of 85 μg proteins from each sample was digested in-solution with trypsin ...
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bioRxiv - Molecular Biology 2021Quote: The final protein concentration was determined by Bradford reagent or Qubit protein assay kit (Invitrogen, #Q33212), and the presence of all three subunits was verified by SDS-PAGE ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentrations of lysate samples were determined (Pierce BCA Protein Assay, Thermo Fisher Scientific, Germany; 23227), the concentration then was adjusted from 20 to 130 μg/ml ...
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bioRxiv - Pathology 2020Quote: ... Protein extraction was carried out using T-PER™ Tissue Protein Extraction Reagent (ThermoFisher Scientific, #78510) The blots were developed using ECL immunochemical detection kit (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration of lysates was determined by Pierce™ BCA protein assay kit (Thermo Fisher Scientific) and same protein quantities were separated on a 10% SDS-PAGE gel under reducing conditions and transferred to 0.45 μm PVDF blotting membrane (GE healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... Protein concentrations for designs lacking aromatic amino acids were measured by Qubit protein assay (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... data were normalized for total protein content of each well using the Bradford protein assay (Thermofisher). Seahorse measurements were performed with 4-6 technical replicates and these experiments were repeated four times.
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bioRxiv - Molecular Biology 2020Quote: The protein synthesis assay was conducted using Click-iT Plus OPP Protein Synthesis Assay Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... After quantification of the total extracted proteins by a Pierce 660 nm Protein Assay (Thermofisher, 22660), 0.5 μg of normalized total proteins from each sample were analyzed by 12% SDS–PAGE and blotted to a PVDF membrane ...
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bioRxiv - Cell Biology 2019Quote: ... Total protein concentration was measured using a Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) and used to normalise sample loading.
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bioRxiv - Cell Biology 2020Quote: ... Protein quantification was completed using a Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific 23225). Protein loading mixes were prepared at 10-25ug samples in MilliQ H2O ...
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bioRxiv - Genetics 2019Quote: Protein analysis include Western Blot for which extracted proteins using the RIPA Buffer (89901 Thermo Scientific) complemented with protease inhibitor cocktail (Roche 06538282001 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins extracts were quantified using the Pierce™ BCA Protein Assay kit (ThermoFisher Scientific, Monza, Italy) and stored in aliquots at −80°C until use.
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bioRxiv - Neuroscience 2019Quote: ... Protein levels were quantified using the bicinchoninic acid protein assay (Pierce BCA kit, Thermo Fisher Scientific) and equal protein concentrations were loaded on acrylamide gels ...
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bioRxiv - Genetics 2019Quote: ... Protein concentrations were measured using BCA Protein Assay kit (NO.23227, Thermo Scientific, Rockford, Illinois, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration of ES products was determined using the Pierce BCA™ Protein Assay kit (Thermofisher). To collect cercarial ES products ...
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bioRxiv - Genetics 2019Quote: ... Protein concentration was measured using Pierce BCA protein assay kit (Thermo Fisher Scientific, MA, USA PI23227) following manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... 30 μg of protein was resolved on NuPAGE-Bis-Tris 4–12% mini protein gels (Invitrogen), which were transferred on to polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2021Quote: ... Co-IP was performed using the Dynabeads-Protein A or Protein G Immunoprecipitation kit (Life Technologies, rabbit antibody with Protein A ...
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bioRxiv - Physiology 2021Quote: ... Protein concentration was measured using a commercially available protein determination kit from Thermo Scientific (catalog # 23225), which is based on the bicinchoninic acid method ...
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bioRxiv - Physiology 2021Quote: ... Protein concentrations were determined using a commercial kit (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific), BSA standards (2 mg/ml in 0.9% saline ...
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bioRxiv - Immunology 2021Quote: ... The protein concentrations were determined using the Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: Proteins were resolved by standard SDS-PAGE or NuPAGE 4-12% Bis-Tris Protein Gels (Invitrogen) before transfer onto Immobilon-PVDF or Nitrocellulose transfer membranes (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... The protein concentration of the lysates was quantified using Pierce BCA Protein Assay Kit (Thermo Scientific). 10-50μg of proteins were loaded to SDS-PAGE gels and transferred and transferred to nitrocellulose membranes for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: Protein concentrations were determined using absorption at 280nm and the Pierce BCA Protein-Assay (Thermo Scientific). Absorption was measured using a Multiskan GO Microplate Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... 100 µg protein from each sample was immunoprecipitated by incubation with protein-G Dynabeads® (Invitrogen) coupled to a rabbit anti-NRP2 antibody (clone 3366 ...
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bioRxiv - Physiology 2021Quote: ... and protein concentration was determined using Pierce™ BCA Protein Assay Kit (23225, Thermo Scientific™) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentration in lysates was determined using Pierce BCA Protein Assay Kit (Thermo Scientific, 23227). The protein concentration in the lysates was 1.74μg/μl ...
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bioRxiv - Cancer Biology 2021Quote: ... Total protein in the supernatant was quantified using the BCA Protein Assay Kit (Thermo Fisher Scientific). Samples were denatured in NuPAGE LDS Sample Buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein content in supernatant was measured using the Pierce BCA Protein Assay (Thermo Scientific, #23225). For each sample ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The protein concentration was detected by Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) and adjusted to 2mg/mL.20µg of the lysates were separated by 10% SDS-PAGE and then transferred onto a PVDF membrane in an ice bath at 300mA for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein content was quantified with BCA Protein Assay kit according to manufacturer’s instructions (Thermo Fisher, #23227) and 20 mg of protein was mixed with 2x Laemmli buffer (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 200 μg protein from each sample was immunoprecipitated by incubation with protein-G Dynabeads® (Invitrogen) coupled to a rabbit anti-α5-integrin antibody (clone 4705S ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentration in the supernatant was quantified with a Pierce BCA protein assay kit (Invitrogen). Fifty micrograms of protein were mixed with Laemmli SDS sample buffer (6× ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein concentration was confirmed using a Pierce BCA protein assay kit (Thermo Scientific, cat #23225). Equal amounts of protein samples were mixed with SDS Laemmli loading buffer ...
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bioRxiv - Microbiology 2020Quote: Protein concentration from cell lysates were quantified by BCA (Pierce BCA Protein Assay Kit, Thermo Scientific). Protein lysates were obtained by lysing cells in 0.1% SDS and protease inhibitor cocktail tablets (Roche #04693124001) ...
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bioRxiv - Immunology 2020Quote: ... 40 uL of a 1:1 mixture of protein A and protein G magnetic Dynabeads (Invitrogen) was added to each well and rocked at 4°C for 4 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration in each fraction was determined (Pierce BCA Protein Assay, ThermoFisher Scientific, Waltham, MA, USA). Subcellular fractions were prepared for electrophoresis by addition of 4x Bolt LDS sample buffer and reducing agent (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A protein concentration assay was performed on supernatant (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific) to inject the same amount of proteins for each sample (L ...
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bioRxiv - Neuroscience 2022Quote: Proteins were extracted from iPSC using N-PER™ Neuronal Protein Extraction Reagent (Thermo Fisher Scientific) supplemented with protease (cOmplete™ Mini Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using the Pierce™ BCA Protein Assay Kit (23225; Thermo Fisher Scientific). After sample protein separation using 10% SDS-PAGE gels (40 μg per sample for all blots) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and separated by SDS-PAGE on a 12% polyacrylamide gel ...
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bioRxiv - Biochemistry 2022Quote: Protein concentrations were measured using the Pierce 660 nM Protein Assay (Thermo Fisher Scientific, Waltham, MA), and samples were normalized for equal protein loading prior to separation on 10% SDS-PAGE gels ...