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Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Total protein concentration was determined using Pierce BCA protein assay kit (Catalog# 23225, Thermo Scientific), and equivalent protein fractions were diluted in cell lysis buffe ...
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bioRxiv - Immunology 2024Quote: ... and quantified for protein concentrations using the Pierce BCA protein assay kit (Thermofisher, Cat# A55864) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Protein quantification was performed by using the Pierce BCA protein assay kit (Thermo Scientific, 23227) according to the user manual ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using the bicinchoninic acid assay (BCA protein assay, Thermo Fisher Scientific). Glut-1 and catalase proteins were separated on a 4% to 20% Tris-Glycine gel (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined with the Pierce™ BCA Protein Assay Kit (Thermo Scientific 23225). 15 µg of protein sample was run on precast 4-15% gradient Tris-Glycine gels (BioRad 4561084 ...
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bioRxiv - Cancer Biology 2024Quote: ... and the total protein concentration quantified with the Micro BCA Protein Assay Kit (Thermo Scientific). Lysates were prepared in SDS sample buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein concentration was determined by spectrophotometry using Pierce™ BCA Protein Assay Kits (ThermoFisher Scientific). Equal amounts (40 μg ...
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bioRxiv - Cell Biology 2024Quote: ... the immunoprecipitates were conjugated with protein G Beads (Dynabeads Protein G, Thermo Fisher Scientific 10004D), eluted in Laemmli buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured with the Pierce TM BCA Protein Assay Kit (ThermoFisher Scientific, USA). Proteins were separated by SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... and protein concentration was measured with the Pierce BCA Protein Assay Kit (Thermo Scientific, 23225). Lysates were resolved on SDS-PAGE and transferred electrophoretically to 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Protein band sizes (kDa) was visualized using the SeeBlue Plus2 pre-stained protein standard (ThermoFisher). For western blot ...
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bioRxiv - Cell Biology 2024Quote: ... Total protein was quantified with a Pierce BCA Protein Assay Kit (23227, Thermo Fisher Scientific) and diluted to 0.4 µg/µL final concentration including lx Master Mix (from EZ Standard Pack l (PS-ST0lEZ-8 ...
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bioRxiv - Bioengineering 2024Quote: Protein concentrations were obtained with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Catalog # 23225), and purified protein was frozen in aliquots on dry ice ...
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bioRxiv - Neuroscience 2024Quote: ... and protein concentrations were determined with the BCA protein assay kit (23235, Thermo Fisher Scientific). Thirty micrograms of total cell lysate were loaded on 10% SDS–polyacrylamide gel electrophoresis gels ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was determined using a Pierce BCA Protein Assay Kit (ThermoFisher, Cat. No: 23227) and standardized ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein concentrations were measured using the Micro BCA Protein Assay Kit (#23235, ThermoFisher Scientific). The protein concentration determined for each individual retina varied from 4µg/µL to 5µg/µL ...
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bioRxiv - Neuroscience 2024Quote: ... Protein levels were quantified by the BCA Protein Assay Kit (Thermo Fisher Scientific, Cat# 23223). Samples were split and electrotransferred using 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels and the PVDF membrane Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration in each sample was determined by BCA protein assay (Thermo Fisher Scientific) and samples were denaturated in 4x Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined with the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23225). Lysates were separated by SDS-PAGE and blotted onto a nitrocellulose membrane (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were measured using a Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were determined and normalized using a BCA protein assay (Thermo Fisher Scientific, 23225). Equal amounts of protein by weight were combined with Laemmli buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Protein concentration was quantified using the bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), and equal quantities of protein were mixed with Laemmli buffer or 5x Pierce Lane Marker Reducing Sample Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Zoology 2024Quote: ... Protein concentration in each extract was measured using PierceTM BCA Protein Assay Kit (Thermo Scientific). Laemmli buffer (Biorad ...
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bioRxiv - Microbiology 2024Quote: ... Total protein was quantified using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific, USA). Calprotectin was normalized to initial stool weight (ng calprotectin/mg stool).
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bioRxiv - Molecular Biology 2024Quote: ... protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific Inc, 78501), and Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific Inc ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Inc, 23225), and 10 μg electrophoresed in 4–12% Bis-Tris denaturing gels (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined using BCA assay (23225, Pierce™ BCA Protein Assay Kit, Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... We combined 4 mg of Cas9 Nuclease protein (TrueCut Cas9 Protein V2, Thermo Fisher Scientific), 5 mL of Neon Buffer R (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Dissolved protein was analyzed for protein concentration by micro-scale BCA assay (Micro BCA Protein Assay Kit #23225; Thermo Fisher Scientific Inc., Waltham, MA, USA). Lysates measured at 50 μg were separated using 10% sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... Intensities of specific bands were normalized to a protein loading control or to the total protein levels marked using MemCode™ Reversible Protein (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Bioengineering 2021Quote: ... 16 Protein concentration of the extracts was estimated using a BCA protein assay kit (Pierce™ BCA Protein Assay Kit, ThermoFisher Scientific, Oakville, ON, Canada) as per supplier’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Intensities of specific bands were normalized to a protein loading control or to the total protein levels marked using MemCode™ Reversible Protein (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Bioengineering 2019Quote: ... they were further incubated with a secondary HRP-conjugated goat anti-rabbit IgG antibody for 2 hours at room temperature and imaged using a supersignal west pico enhanced chemiluminescence kit (Thermo Fisher Scientific, Suwanee, GA,USA). The signals were visualized with UVP software.
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bioRxiv - Neuroscience 2022Quote: ... Detection was carried out using enhanced chemiluminescent detection (ECL) according to the manufacturer’s instructions (Western Lightning Plus-ECL or SUperSignal West Femto Maximum Sensitive Substrate; Thermo Fisher Scientific, St Leon-Rot, Germany). Immunoblots were scanned using ECL Chemostar (Intas Science Imaging ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were washed in 1X T-TBS and incubated in HRP-conjugated secondary antibody to be detected by the enhanced chemiluminescence SuperSignal ®West Femto Maximum Sensitivity Substrate (Thermo Scientific, Cat No. #34095). βActin and/or GAPDH were used as cellular loading controls.
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bioRxiv - Pathology 2024Quote: Liquid chromatography with tandem mass spectrometry was then used to quantify cAMP using an orbitrap Fusion Lumos mass spectrometer interfaced to an Ultimate3000 HPLC system (Thermo Scientific, West Palm Beach, FL) and an Atlantis C18 column (2.1×150mm ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were washed 3 times with TBS-T for 10 minutes at room temperature and then developed with Pierce SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher, Cat. No. 340940). Blots were visualized with a BioRad GelDoc system.
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bioRxiv - Cancer Biology 2023Quote: ... and chemiluminescent HRP substrates (Supersignal West Pico PLUS, Thermo Fisher Scientific; Pierce ECL Plus, Thermo Fisher Scientific; or Immobilon ECL Ultra, Millipore) (Thermo Fisher Scientific, RRID:SCR_008452).
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bioRxiv - Microbiology 2023Quote: ... Blots were again washed for 30 min and visualized using the Amersham ECL reagent or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, Waltham, MA, USA) and ChemiDoc MP (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatants were then transferred into fresh Eppendorfs and frozen at −80°C (for radiolabeled glucose to protein) or their protein concentrations calculated (Western blot) using a Pierce BCA Protein Assay kit (Thermo Scientific, Cat#23225, Waltham, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... pellets were reconstituted in 50[µl of 0.1[N NaOH and kept for protein quantification using a BCA protein assay kit (Thermo Scientific Pierce BCA Protein Assay Kit, PI23227). Dialysate concentrations are expressed as ng/ml and tissular concentrations as ng/mg of total proteins.
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bioRxiv - Neuroscience 2023Quote: Total protein levels in cortex homogenates were quantified using a Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Protein amounts were determined using a Qubit4 Fluorometer and Qubit Protein Assay kit (Thermo Fisher Scientific). GUS activity was calculated in (nmol 4-MU ...
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bioRxiv - Microbiology 2020Quote: ... Lysate protein concentrations were determined using a BCA protein assay kit according to manufacturer’s instructions (ThermoFisher).
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was collected for protein quantification using Pierce™ BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Developmental Biology 2020Quote: Changes in protein expression were monitored using Click-iT Protein Synthesis Assay kit (Thermo Fisher Scientific). Oocytes and 2 cell embryos were cultured in M16 medium with the methionine analogue HPG at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... and DNA-protein complexes were pulled down using Protein A/G agarose beads (Thermo Fisher, 20423). Agarose beads were washed with the following buffers ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined in the supernatant by BCA Protein Assay Kit (Thermo Fisher Scientific, #23227). 100 μg of protein was transferred into a new tube and the final volume was adjusted to 100 μL with 100 mM triethylammonium bicarbonate (TEAB) ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants were collected for measuring the protein concentration with BCA Protein Assay Kit (ThermoFisher Scientific). Cytosolic and nuclear extracts were prepared using NE-PER reagents (ThermoFisher Scientific ...