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Citations for Thermo Fisher :
5851 - 5900 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... 1 mL of chloramphenicol-treated cells were stained for 15 minutes with 300 nM SYTOX Orange Nucleic Acid Stain (Invitrogen). The excess of SYTOX Orange was washed in tethering buffer before proceeding with FCS experiments ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... we then removed the mesh and marbles and transferred the remaining water and dust suspension to acid-washed 1 L bottles (Nalgene Nunc International Corporation ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA encoding full-length human ADNP2 (amino acid residues 1–1131) was PCR amplified and cloned into a pFast-Bac-derived vector (Invitrogen) in frame with an N-terminal His6-tag ...
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bioRxiv - Molecular Biology 2024Quote: ... Neuroblastoma SH-SY5Y cells were cultured in the same conditions and the same medium but supplemented with 1 mM of non-essential amino acids (Gibco). The total culture surface was 4500 cm² ...
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bioRxiv - Biochemistry 2023Quote: ... The gels were stained in 1xTBE with 1:20,000 SYBRTM Gold nucleic acid stain (Thermo Fisher Scientific Corp, Waltham MA) for 30 minutes and imaged on a ChemiDoc MP system (Bio-Rad Laboratories Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... The gels were stained in 1xTBE with 1:20,000 SYBRTM Gold nucleic acid stain (Thermo Fisher Scientific Corp, Waltham MA) for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Fisher Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Plant Biology 2022Quote: ... a 1/200 dilution of cells were transferred to fresh media containing 1X SYBR Green I Nucleic Acid Gel Stain (Invitrogen, now Theremo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: Cytotoxicity measurement was performed by incubating BL21 Star (DE3) or HEK293T cells with 1 μM SYTOX Blue nucleic acid stain (Invitrogen) for 5 min before imaging by a 405 nm laser irradiation.
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bioRxiv - Microbiology 2023Quote: ... The cleared lysates were passed through a 0.45-μm filter and incubated with 1 ml of Ni-NTA (nitrilotriacetic acid) resin (Thermo Scientific) at 4°C over-night (O/N ...
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bioRxiv - Biochemistry 2023Quote: ... DNA migration was detected by incubating the gel with SYBR Gold Nucleic Acid Gel Stain (Invitrogen #S11494, 1/10, 000) in 1X TBE for 5-10 min and reveal using a Chemidoc MP Immaging System (BIO-RAD) ...
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bioRxiv - Systems Biology 2023Quote: ... The samples were resuspended in 500 μL of Acid-Phenol:Chloroform (with IAA, 125:24:1, pH 4.5, Thermo Fisher Scientific), 500 μL of Buffer B (200mM NaCl (DOT Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Purification was performed using 1 mL of Ni2+-nitriloacetic acid (NTA) resin (HisPurTM Ni-NTA resin, Thermo Fisher Scientific Co.) loaded onto 10 mL-Pierce™ disposable columns ...
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bioRxiv - Neuroscience 2024Quote: ... solutions prior to being placed in developer solution (2.5% sodium carbonate, 0.1% ammonium nitrate, 0.1% silver nitrate, 1% tungstosilicic acid, 0.7% formaldehyde (Thermo Fisher Scientific, MA, USA). Slides were treated with 0.5% acetic acid to stop the reaction ...
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bioRxiv - Microbiology 2024Quote: ... bacteria were adjusted to an OD600 of 0.05 and mixed with 10 % normal human serum (NHS) (serum was prepared as described in41) in the presence of 1 µM SYTOX Green Nucleic Acid stain (ThermoFisher) in RPMI ...
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bioRxiv - Cell Biology 2024Quote: ... and fibroblasts were cultured in a basic media (DMEM/F12, +10% FBS, +1% MEM Non-Essential Amino Acids Solution 100x (MEM-NEAA, Gibco), +1% L-Glutamine (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... followed by an anti-mouse secondary antibody (V5: 1 hr, RT, 1/500; Invitrogen, Cat #A21200; FLAG: 2 hr, RT, 1:200; Invitrogen, #A11004) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Resuspended bacteria were then lysed (2 × 108 total CFU) by a 1:1 addition of 1% dodecyltrimethylammonium bromide (DTAB) (AC409310250; Fisher Scientific) for 5 min with agitation ...
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bioRxiv - Cancer Biology 2021Quote: ... using a binary solvent system of 2% acetonitrile with 0.1% formic acid (Solvent A) and 80% acetonitrile with 0.1% formic acid (Solvent B) in an Easy nLC-1000 system (Thermo Fisher Scientific). 2 μL of peptide solution was loaded using Solvent A onto an Acclaim PepMap100 C18 trap column (2 cm x 75 μm inner diameter) ...
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bioRxiv - Systems Biology 2020Quote: ... water with 0.1% formic acid (v/v) and acetonitrile with 0.1% formic acid (v/v) were purchased from Fisher Scientific (Leicester, U.K.). Methanol and isopropanol were purchased from Honeywell (Charlotte ...
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bioRxiv - Neuroscience 2022Quote: ... Mobile phases A and B were with 0.1% formic acid in water and 0.1% formic acid (Fisher Scientific; Cat#: A11710X1-AMP) in ACN (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... of acetonitrile (with 0.1 % formic acid) in solvent (0.1% formic acid in water) for 80 min and sprayed directly into the Orbitrap instrument (Thermo Fisher Scientific). MS1 scans were collected at 120,000 resolutions in a 320–2,000 m/z range with 100 ms max injection time (IT ...
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bioRxiv - Biochemistry 2022Quote: ... formic acid (FA), difluoroacetic acid (DFA), trifluoracetic acid (TFA), Zeba™ Spin Desalting Columns (7K MWCO, 0.5 mL) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Plant Biology 2024Quote: ... jasmonic acid (JA) and abscisic acid (ABA) by ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS, Q-Exactive, ThermoFisher Scientific)26.
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bioRxiv - Immunology 2024Quote: ... All LC/MS solvents and reagents were the highest purity available (water, acetonitrile, acetic acid, boric acid, sodium hydroxide) and were purchased from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: Skin tissues collected from newborn Foxn1−/− (Cby.Cg-Foxn1
/cmdb) and Foxn1+/+ (C57BL/6; B6) mice were incubated in dispase (6 U/ml; Life Technologies) overnight at 4°C ... -
bioRxiv - Biochemistry 2021Quote: ... were incubated without ligand or with EGF (6 μM) or EREG (6 μM) in buffer A containing disuccinimidyl suberate (DSS) at 62.5 μM (ThermoFisher Scientific) for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Microbiology 2022Quote: 293T-ACE2 and HT1080-ACE2 cells were seeded in 24-well plates and 6-well plates respectively to achieve 70% confluency after 4-6 hours and then transfected with Lipofectamine RNAiMAX (Thermofisher) using the indicated dsiRNAs (IDT ...
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bioRxiv - Cancer Biology 2020Quote: ... 293T cells were transiently transfected in 6-well plates with 6 individual IFNLR1-gRNA plasmids using Lipofectamine 3000 (Life Technologies). The cells were harvested 4 days post-transfection and subjected to fluorescence-activated cell sorting (FACS ...
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bioRxiv - Cancer Biology 2022Quote: ... and ran on QuantStudioTM 6 Flex Real-Time PCR System using QuantStudioTM 6 and 7 Flex Real-Time PCR software v1.0 (Applied Biosystems). Relative gene expression levels were quantified using β-actin or human TBP as housekeeping genes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Pro-inflammatory cytokine (IL-8) and (IL-6) release was determined using the IL-6 and IL-8 ELISA kit (Invitrogen) according to the manufacturer’s instructions (ThermoFisher).
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bioRxiv - Genetics 2021Quote: ... treated wild-type C57BL/6 and 12-week-old Rad1 CKO mice were subjected to phosphopeptide enrichment and 6-plex TMT (ThermoFisher) labeling (Sims et al. ...
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bioRxiv - Pathology 2022Quote: ... The concentrations of IL-6 and VEGFA were determined using an enzyme-linked immunosorbent assay (Mouse IL-6 ELISA kit, ThermoFisher; Mouse VEGF-A DuoSet ELISA kit ...
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bioRxiv - Immunology 2024Quote: ... primary Rhinolophus ferrumequinum bat cells were seeded in 6-well plate at a density of 6×105 cells/well with DMEM containing 10% FBS (Gibco). After 12 h ...
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bioRxiv - Immunology 2023Quote: ... The recombinant bacmid DNA was transfected into the Sf9 insect cells seeded in 6-well plates at 0.5 × 106 cells/ml using Fugene 6 reagent (Invitrogen protocol). At 72 hours post-transfection ...
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bioRxiv - Microbiology 2023Quote: Interleukin-6 (IL-6)_ and interleukin-12 (IL-12_ expression levels were detected by ELISA Kits (Thermo Fisher Scientific, USA); the samples analysed via ELISA were either plasma drawn from mice or cell culture supernatants ...
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bioRxiv - Immunology 2024Quote: ... IL-6 levels in the supernatant and plasma were determined using a high sensitivity IL-6 ELISA Kit (BMS603HS, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2020Quote: ... at 37 °C with 5 % CO2 with 1 % penicillin/streptomycin (PS, 5000 U.mL-1 and 5000 μg.mL-1respectively; Life Technologies) and supplemented with 1% non-essential amino acids (Life Technologies).
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bioRxiv - Cell Biology 2021Quote: ... the stromal layer was washed with PBS and was subject to mild trypsinisation with 1:5 dilution of 0.25% Trypsin 1 mM EDTA (Thermofisher) to remove attached residual leukaemic cells before completely detaching stromal cells with 0.25% Trypsin-EDTA.
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bioRxiv - Systems Biology 2021Quote: ... Dried lipid fractions (~5 mg) were dissolved (isopropanol/dichloromethane 1:1, 300 μL) for accurate mass LC-MS (ThermoFisher Q exactive with Dionex Ultimate 3000 sample handler and Waters acquity UPLC BEH C18 with 1·7 μm particle size LC column ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions contained 1 μl of 1:10 diluted lysate and 5 μl premixed AmpliTaq-Gold (0.3U, Life Technologies) with 0.26 μM of target-specific- and an additional fluorescent forward primer ...
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bioRxiv - Biochemistry 2020Quote: SHSY5Y cells were obtained from the Duke University Cell Culture Facility and were maintained at 37 °C with 5% CO2 in DMEM:F12 (1:1; Gibco) supplemented with 10% FBS (Sigma Aldrich ...
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bioRxiv - Physiology 2021Quote: ... Frozen tissues were mechanically homogenized with 1 stainless steel bead (5 mm) in 1 ml Trizol (Thermo Fisher Scientific) by shaking for 50 s at 30 Hz (TissueLyser ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 min) and resuspended in 1 ml PBS containing 8 µl UltraPure BSA (50mg ml−1; AM2616, ThermoFisher Scientific) and filtered over Flowmi 40µm cell strainers (VWR ...
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bioRxiv - Genomics 2021Quote: ... nuclei were incubated for 1 hour in a solution containing 5% FBS and 1:500 primary antibody (H3K9me3; Invitrogen rabbit polyclonal 49-1008 or H3K9Ac ...
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bioRxiv - Cell Biology 2020Quote: ... All cells were maintained in 1:1 Ham’s F12 Dulbecco’s modified Eagle’s medium (FDV) containing 5% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 5ml/500ml 100X penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific and goat anti-rabbit-HRP ...