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Citations for Thermo Fisher :
5801 - 5850 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2 mM glutamine and 1% penicillin/streptomycin (Life Technologies, Thermo Fisher Scientific). HUVECs were isolated from donors using previously described methods (59) ...
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bioRxiv - Cell Biology 2023Quote: ... sections were incubated in 2 mM POPO-1 nuclear counterstain (Life Technologies) diluted in PBS containing 0.05% saponin (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... or with three custom targeting sgRNAs (Invitrogen, Supplementary Tables 1 and 2).
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bioRxiv - Cell Biology 2023Quote: ... (1) E8 medium supplemented with 2% E8 supplement (50x) (Thermo Fisher Scientific), 1% Penicillin Streptomycin (5000U/ml ...
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bioRxiv - Microbiology 2023Quote: ... Diamidino-2-phenylindole (DAPI) (Thermo Scientific, #62247, 1:1000 dilution for IFA); Mounting medium (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... 1-2 µg of RNA was treated with DNAse I (ThermoFisher Scientific), and the cDNA was prepared using the RevertAid First Strand cDNA Synthesis kit (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... containing 1% BSA and 2 mM EDTA (Thermo Fisher Scientific, Waltham, MA) was added.
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies were Alexa Fluor 594 (1:750, 2 hrs; Invitrogen A11008) and Alexa Fluor 488 (1:750 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged at a ratio of 1:2 using TrypLE (Gibco). U2OS cells were a gift from the Chang lab and grown in McCoy’s 5A (modified ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged at a ratio of 1:2 using TrypLE (Gibco). Stem cell line H1 were purchased from WiCell (Cat # WA01) ...
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bioRxiv - Neuroscience 2023Quote: ... 2) rabbit anti-pT217-tau at 1:200 (cat # 44-744, ThermoFisher). The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human tau that contains threonine 217 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 % penicillin-streptomycin and 100 µM 2-mercaptoethanol (all from Thermo Fisher), and further supplemented with 100 ng/mL Noggin (Bio-techne) ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM L-glutamine and 1 mM sodium pyruvate (Thermo Fisher, #11360039), 100 µg/ml of Normocin (InvivoGen ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 2 ug/µL BSA (Cat No. B14, ThermoFisher Scientific), and 1 µL of 10 ng/µL DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were selected with 2 µg mL-1 puromycin (Thermo Fisher Scientific) for 3 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary monoclonal transglutaminase-2 antibody (CUB7402, Thermo Fisher #MA5-12736, 1/15000) was diluted in 0.5 (m/v ...
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bioRxiv - Microbiology 2024Quote: ... mouse monoclonal anti-BrdU (Thermo Fisher, B35128, 1:50, 2 µg/mL). Secondary antibodies and the optimal dilutions and concentrations used are as follows ...
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bioRxiv - Cell Biology 2024Quote: After washing 2 times with ice-cold 1× PBS (14080-055, Gibco), cells were trypsinized or scrapped with a rubber policeman ...
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bioRxiv - Molecular Biology 2020Quote: ... and shRNA plasmids or pCDH plasmids by PEI-40000 with the ratio of 5: 1: 5 in opti-MEM (Gibco, Thermo Fisher Scientific). Virions were collected after 48 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... The combined samples were dissolved in loading buffer [1% ACN and 1% formic acid (FA)] and analyzed using an EASY-nLC 1200 ultra-performance liquid chromatography system (Thermo Fisher Scientific, San Jose, CA, USA) equipped with a self-packed capillary column (75 μm i.d ...
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bioRxiv - Developmental Biology 2023Quote: ... were cultured in mEC medium (0.1% bovine brain extract (BBE)/10% FBS/1%penicillin-streptomycin/1% Non-Essential Amino Acid (NEAA)/Dulbecco Modified Eagle Medium (DMEM) (DMEM Cat#: 41966-029, Gibco; BBE Cat#: CC-4098, Lonza BioScience). All cells were cultured at 37 °C in a humidified atmosphere with 5% CO2 (HDLECs and HUVECs ...
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bioRxiv - Cancer Biology 2020Quote: ... The medium was changed every 2-3 days and organoids were passaged every 2-4 weeks by dissociation with 1 ml of TrypLE Express (Life Technologies) at 37°C for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with KIF-encoding plasmid and empty vector pcDNA3.1 (+) (1 µg total DNA) using 2 µl Lipofectamine 3000 Reagent and 2 µl P3000 (Thermo Fisher Scientific). hTERT-RPE1 cells stably expressing SMO-tRFP were transfected a day after seeding and IMCD3 cells expressing mCherry-Arl13b were reverse transfected at the time of seeding ...
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bioRxiv - Neuroscience 2020Quote: ... A square glass window (2 mm x 2 mm) of No 1 cover glass (0.13 to 0.17 mm thick, Thermo Fisher Scientific, USA) was placed over the craniotomy and the edges were sealed with cyanoacrylate glue (3M ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Neuroscience 2024Quote: Intracellular ROS in BV-2 cells was measured by incubating cells with 1 µM cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Invitrogen, D399) at 37°C for 30 min.
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bioRxiv - Cancer Biology 2023Quote: ... was pre-complexed at 5µg/mL with PE-conjugated goat anti-Human IgG diluted at 1/100e (Supplementary Table 2) for 2 h at room temperature in PBS CaCl2 MgCl2 (14040133, ThermoFisher Scientific). 200 000 T-ALL cells pre-stained with cell surface markers were incubated with this complexe ...
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bioRxiv - Genomics 2023Quote: ... were added into a fresh 2 mL microcentrifuge tube and washed with 1 mL of NPB using a DynaMag-2 magnet (#12321; ThermoFisher Scientific) for a total of three washes (1 min incubation/each) ...
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bioRxiv - Cell Biology 2023Quote: ... First, cell detachment was attained using a cell dissociation solution (at 2:2:1 ratio, Cell Dissociation Buffer (Thermo Fisher Scientific): RPMI 1640 ...
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bioRxiv - Genomics 2023Quote: ... total RNA from young livers were spiked with 2 µl of a 1:100 dilution of ERCC Ex-fold Mix 1 while that from old livers were spiked with 2 µl of 1:100 dilution of ERCC Ex-fold Mix 2 (Thermo Fisher). Exfold ERCC spike-ins are provided in two mixes ...
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bioRxiv - Cancer Biology 2023Quote: ... were mixed in a 3:1 with sample buffer (NuPAGE™ LDS Sample Buffer (4X) + 2-Mercaptoethanol in 2:1) and loaded onto a precast Gel (NuPAGE™ 4-12% Bis-Tris Gel, Invitrogen). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium for HT-1197 and HT-1376 was supplemented with 1x non-essential amino acids and 1 mM sodium pyruvate (Gibco). All culture media were supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Developmental Biology 2021Quote: ... A second RNA extraction was performed by adding 250uL of Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) (Ambion, Cat#AM9720) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was then extracted by mixing samples with Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) (Thermo Fisher, AM9722), incubating at 65°C ...
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bioRxiv - Cancer Biology 2020Quote: ... U87 and SH-SY5Y cells were grown in DMEM additionally supplemented with 1% non-essential amino acids (Invitrogen, Karlsruhe, Germany).
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bioRxiv - Microbiology 2021Quote: ... media for Calu-3 and Caco-2 cells were supplemented with 1x non-essential amino acid solution (from 100x stock, PAA) and 1 mM sodium pyruvate (GIBCO). Cell lines were validated by STR-typing ...
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bioRxiv - Physiology 2020Quote: ... EDL from both legs were dissected and maintained in 4°C Krebs–Henseleit buffer supplemented with 1×MEM amino acid mixture (Invitrogen) and 25mM glucose ...
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bioRxiv - Neuroscience 2020Quote: The cDNA sequence corresponding to amino acids 1-192 of rab26 was amplified by PCR and cloned into the pET28a (Invitrogen) vector for protein expression ...
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bioRxiv - Neuroscience 2021Quote: ... Perk+/+ and Perk-/- mouse embryonic fibroblasts (MEFs) were cultured in DMEM supplemented with 1% MEM non-essential amino acid (Gibco). Ire1α+/+ ...
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bioRxiv - Bioengineering 2022Quote: ... resuspended in RPMI growth media (RPMI supplemented with 10% FBS, 1x non-essential amino acid solution [Gibco 11140050], 1 mM sodium pyruvate [Gibco 11360070] ...
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bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 cells were cultured in the same medium with the addition of 1% Non-Essential Amino acids (Gibco). Cells were grown at 37°C with 5% CO2 in a humidified atmosphere ...
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bioRxiv - Immunology 2020Quote: ... modified with 1% (v/v) each of minimum essential media (MEM) non-essential amino acids (Gibco; Grand Island, NY, USA), sodium pyruvate (Gibco ...
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bioRxiv - Genomics 2021Quote: ... Colonies were washed with DMEM/F12 several times and allowed to settle by gravity then were plated in embryoid body formation media (DMEM/F12 supplemented with 2mM GlutaMAX, 1% non-essential amino acids (Thermofisher), 50nM 2-mercaptoethanol (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: ... HPAF-II and EGI-1 cell lines were cultured in MEM media with Earle’s salts and non-essential amino acids (Gibco #10370021), AsPC-1 and COLO 320HSR cells in RPMI (Nacalai Tesque #0517625) ...
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bioRxiv - Cancer Biology 2022Quote: ... peptides in 1% (v/v) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (ThermoFisher Scientific, Inc). After washing with 0.5% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... were incubated in serum or colistin diluted in RPMI buffer in the presence of 1 μM SYTOX Green Nucleic Acid stain (ThermoFisher). Bacteria were incubated at 37 °C under shaking conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... and a cDNA with an internal deletion such that the resultant cDNA encodes amino acids 1-178 and 1025-1099 were digested and subcloned into the NotI and XhoI sites of pcDNA4/TO (Invitrogen) modified to include N-terminal 3×FLAG-2× STREP tags to create plasmids 3×FLAG-2×STREP-Zfp106[Cterm] ...
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bioRxiv - Neuroscience 2023Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap® C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...