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Citations for Thermo Fisher :
5801 - 5850 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Human GAA coding sequence variant GILTco2-m was designed using GeneArt codon optimization algorithm (Thermofisher Scientific). The human GAA coding sequence variant GILTco3-m was designed using a consensus sequence (CCDS32760.1) ...
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bioRxiv - Bioengineering 2020Quote: ... or mouse anti-human CD31 (eBiosciences) followed by Donkey Anti Rat-IgG-Alexa FluorTM 594 (Invitrogen), or Goat Anti Mouse-IgG-Alexa FluorTM 594 (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... 100nM of siCTL or the Human DDX5 siRNA pool were mixed with Lipofectamine 3000 (L3000001, Invitrogen) in Opti-MEM medium according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Scaffolds were then incubated with 250 μg/mL human plasma fibronectin (Cat. No. 33016-015; Gibco) overnight at 37 °C to enhance hydrophilicity ...
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bioRxiv - Cell Biology 2021Quote: Copeptin in human plasma was analysed using the KRYPTOR compact PLUS (Brahms Instruments, Thermo Fisher, DE). Glucagon was measured using human glucagon ELISA from Mercodia ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human TNF-α a well established trigger of HIV-1 reactivation was purchased from Gibco. The PKC activator Bryostatin and Sodium Butyrate (NaBu ...
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bioRxiv - Microbiology 2020Quote: ... Six μg/mL of goat anti-human H+L conjugated Alexa 488 (Thermo Fisher Scientific A11013) together with 8 μM of antifade-46-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2020Quote: ... falciparum was cultured in vitro in human O+ erythrocytes and RPMI medium (Gibco Cat. no. 11875) supplemented with 5% heat-inactivated human serum ...
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bioRxiv - Systems Biology 2021Quote: Human lung AEC A549 (ATCC-CCL 185) were maintained in F12K nutrient medium (Thermo Fisher Scientific) with the addition of 10% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were collected and cytokine levels assed with Human IFNγ ELISA Kit (ThermoFisher Scientific, Vienna, Austria) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The subclass of each IgG antibody was determined using a human IgG subclass ELISA kit (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... Primary human CD14+ monocytes were maintained in Hank’s Balanced Salt Solution (HBSS, #14175095, Thermo Fisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... and human basic Fibroblast Growth Factor (bFGF) (Thermo Fisher Scientific 68-8785-82, 20 ng/mL). LUHMES cells were grown to 80% confluency and passaged 1:6 using diluted trypsin-EDTA (0.25% ...
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bioRxiv - Biochemistry 2022Quote: ... The ORF of human MYBL2/B-Myb isoform 1 (NM_002466.4) was cloned into pcDNA3.1+ (ThermoFisher Scientific) and fused with an N-terminal Flag tag ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-human IL6 Ab concentrations were quantified using the Quant-it protein assay kit (Life Technologies). Ab conjugates were validated on 10% TBE urea denaturing gels and validated on an agarose gel (Supplementary Figure S8) ...
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bioRxiv - Molecular Biology 2022Quote: ... Mouse-anti-human H3K9/14/18/23/27ac (Thermo Fisher Scientific, Carlsbad, CA, USA; 1:500) was used to detect this histone modification in HPdLF and goat-anti-Mouse-Cy5 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... APC anti-human CD25 (17-0259-42) and 2-NBDG (N13195) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... with detection of the complex with goat anti–human IgG Fab (200 µg/well; Invitrogen, #31122).
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bioRxiv - Neuroscience 2022Quote: The human Tara coding sequence was amplified by PCR and cloned into the pPC97 vector (Invitrogen). Host Saccharomyces cerevisiae strain MaV203 cells were co-transformed with pPC97-Tara and human fetal brain cDNA library plasmids cloned in pPC86 (GibcoBRL) ...
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bioRxiv - Genomics 2022Quote: ... mTESR1 is exchanged with the addition of 5 ng/ml Recombinant Human BMP4 (Fisher Scientific, 314BP010). Exposure to BMP4 triggers differentiation of cells and is considered 0 hours for experimental purposes ...
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bioRxiv - Genomics 2022Quote: Beating human iPSCs were switched to Opti-MEM™ Reduced Serum Medium (ThermoFisher Scientific, Waltham, MA). LINC000881 knockdown was achieved by lipofectamine based transfection of antisense LNA GapmeRs designed against LINC00881 versus scrambled oligonucleotide (QIAGEN ...
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bioRxiv - Genomics 2022Quote: ... Beating human iPSCs were switched to Opti-MEM™ Reduced Serum Medium (ThermoFisher Scientific, Waltham, MA). LINC000881 overexpression was achieved by lipofectamine based transfection of LINC00881 plasmid versus bacterial alkaline phosphatase control plasmid using RNAiMAX reagent (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The secreted fusion protein was purified on a CaptureSelect™ human albumin affinity matrix (Life Technologies), and protein eluted by adding 20 mM Tris and 2.0 M MgCl2 ...
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bioRxiv - Immunology 2020Quote: ... 0.05% Tween-20 (PBST) and ALP-conjugated goat anti-human IgG (H+L) antibody (Thermo Fisher) was added into each well and incubated at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... Background binding level in ELISA assays was measured using Human IgG Isotype Control (ThermoFisher #02-7102).
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bioRxiv - Molecular Biology 2021Quote: ... Binding of the antibodies was detected using Alexa fluor 633 conjugated anti-human IgG antibodies (Invitrogen). Expression of the surface-displayed myc-tagged RBDs was detected using a FITC conjugated chicken anti-myc antibody (Immunology Consultants Laboratory ...
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bioRxiv - Immunology 2020Quote: ... 50μL of polyclonal anti-human β2M HRP-conjugated IgG detection antibody raised in rabbits (ThermoFisher Scientific), diluted 1:2500 in 2% BSA (0.2μg/mL) ...
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bioRxiv - Microbiology 2021Quote: ... 0.1 mL media or media containing Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell ...
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bioRxiv - Microbiology 2021Quote: ... Alexa488- or Rhodamine-conjugated goat anti-human secondary antibody was added (1/500 dilution) (Thermo Fisher), and incubated for 2~3 hr at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: Healthy human dermal fibroblasts of neonatal origin (HDFn) were cultured in DMEM GlutaMAX medium (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Genomics 2022Quote: ... human preadipocytes in a 6-well plate were transfected with 6.25ug Cas9 protein (Fisher Scientific, A36498) and 800ng sgRNAs (IDT) ...
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bioRxiv - Immunology 2022Quote: ... silencer select siRNA for human FtH1 and negative control siRNA (4392421 and 4390843 respectively, ThermoFisher Scientific) were used ...
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bioRxiv - Biochemistry 2022Quote: ... and pooled 50-donor human liver cytosol (Fisher Scientific, Hampton NH, Ref: HMMCPL, Lot: PL028-J) were protein normalized ...
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bioRxiv - Cell Biology 2022Quote: ... with <0.9% rabbit brain tissue contaminant) and another tube with human recombinant Thromboplastin (Thermo fisher scientific). Next to remove cells ...
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bioRxiv - Genetics 2019Quote: ... Each gel included a migration standard comprised of Di-I labeled human LDL (L3482, ThermoFisher Scientific) that was diluted in cryoprotectant and stored in frozen aliquots (see recipes) ...
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bioRxiv - Cancer Biology 2019Quote: ... were cultured in Medium 254 (Invirogen, Portland, OR, USA) supplemented with Human Melanocyte Growth supplement (Invitrogen). All cells were cultured at 37°C in a humidified incubator under 5% CO2 ...
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bioRxiv - Immunology 2019Quote: ... Custom synthesized (IDT biotech) human KCNJ15 cDNA was sub-cloned into the PE1A plasmid (Invitrogen, #A10462), followed by gateway transfer of KCNJ15 into the lentiviral vector ...
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bioRxiv - Immunology 2020Quote: ... The fibrinogen used here was isolated from human plasma and conjugated with AlexaFluor-647 (Invitrogen™).
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bioRxiv - Molecular Biology 2021Quote: The human GIPR DNA (Genewiz) with one mutation (T345F) was cloned into the pFastBac vector (Invitrogen) with its native signal peptide replaced by the haemagglutinin (HA ...
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bioRxiv - Cell Biology 2021Quote: ... murine or human serum94 in Dulbecco’s modified Eagle’s medium (DMEM, Life Sciences, Thermo Fisher Scientific, Germany) for 30 min ...
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bioRxiv - Biophysics 2021Quote: Human immortalized keratinocytes (HaCat) were cultured with Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Thermo Fisher Scientific) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biophysics 2021Quote: Human immortalized keratinocytes (HaCat) were cultured with Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Thermo Fisher Scientific) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Human BM-MSC lines were maintained in AmnioMAX™ with C100 supplement (Life Technologies, Carlsbad, CA); these lines were previously confirmed to express characteristic stromal markers (>95% CD90+ ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the LDH CytoTox 96 kit from ProMega (Madison, WI), Urea Nitrogen Test from StanBio Laboratory (Boerne, TX) and human TNF-α from ThermoFisher Scientific (Waltham ...
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bioRxiv - Immunology 2019Quote: ... the human IL-2 ELISA kit was used in accordance with manufacturer’s instructions (Thermo Fisher Scientific). Murine IL-2 ELISA’s were performed using the mouse IL-2 ELISA kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi γ32P-ATP and 350 ng CDK1/Cyclin B Recombinant Human Protein (Thermo Fisher PV3292) in kinase buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Physiology 2021Quote: 2-3 viable human slices were incubated with Fluo4-AM (6 μM, Invitrogen cat. No. F1221) for 1h in 3 mM HEPES buffer (125 mmol/l NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Immortalized human fibroblasts were cultured in DMEM (sigma) supplemented with 1% of penicillin and streptomycin (Gibco) and 10% fetal bovine serum (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... nondiabetic human islets were handpicked to purity and then dispersed with 0.025% HyClone trypsin (Thermo Scientific) for 7 minutes at room temperature before counting with an automated Countess II cell counter or manually by hemacytometer ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-Hec1 (1:1000, human monoclonal) and Anti-phospho-Paxillin (1:100, rabbit polyclonal, pTyr31, Invitrogen). Secondary antibodies diluted in antibody dilution buffer was added along with the conjugated Phalloidin-TRITC (Santa Cruz Biotechnology ...