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Citations for Thermo Fisher :
5751 - 5800 of 7745 citations for Recombinant Human PPIA His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Human jejunal enteroids and colonoids were cultured in Matrigel (75%) domes overlaid with hOGM/1x PenStrep (Gibco) at 37°C ...
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bioRxiv - Biochemistry 2024Quote: Human neuroblastoma SH-SY5Y cells (ATCC) or HEK293 (ATCC) cells were cultured in DMEM/F12 media (Gibco) or DMEM High-glucose (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... and Taqman gene expression assays (human tyrosinase [TYR] Hs00165976_m1; mouse TH Mm00447557_m1; Slc6A3 [DAT] Mm00438388_m1; Slc18a2 [VMAT2] Mm00553058_m1, Applied Biosystems) using standard procedures in a 7900HT Fast Real Time Instrument (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were isolated using the DynabeadsTM untouched human CD8 T-cell isolation kit (Invitrogen), stimulated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco™ ...
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bioRxiv - Developmental Biology 2024Quote: ... and adapter ligation were performed using an Ion AmpliSeq Transcriptome Human Gene Expression Kit (Thermo Fisher Scientific). The cDNA library was purified using Agencourt AMPure XP Reagent (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... Human primary Müller cells (P3) at 80% confluency were transfected with MT1 (assay ID: s194623, ThermoFisher Scientific), AKAP12 (assay ID ...
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bioRxiv - Genetics 2024Quote: Human embryonic kidney (HEK) 293T cells were maintained in DMEM supplemented with 10% fetal calf serum (Gibco). Cells were seeded with 300 000 cells/well in 6-well plates and transfected 24 hours later with 1 µg of each minigene construct mixed with 3 µL of Lipofectamine 2000 Reagent (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Human skin: Fat and lower dermis was cut away and discarded before dispase (2 U/ml, Gibco) digestion for 20h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... falciparum blood-stage parasites were cultured in 2.5% hematocrit human RBC in malaria culture media (MCM) comprising of 10.43g RPMI 1640 powder (Gibco), 25ml 1M HEPES (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... The silencer select siRNA targeting human ERK3 and the Silencer non-targeting Control #1 were purchased from Ambion.
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bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
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bioRxiv - Cell Biology 2020Quote: Normal human fibroblast cell line (AG08469, Coriell Institute for Medical Research) was maintained in Minimum Essential Medium (Gibco) and supplemented with 15% FBS (HyClone) ...
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bioRxiv - Cell Biology 2020Quote: Silencer Select siRNAs against human SNX4 and VPS35, and nontargeting control “scrambled” siRNA (predesigned, 4,390,844) were purchased from Ambion® (Thermo Fisher Scientific) ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... elegans γ-tubulin complex was reconstituted by co-expression in human FreeStyle 293-F cells (Thermo Fisher Scientific). The GIP-2 and γ-tubulin (TBG-1 ...
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bioRxiv - Genomics 2021Quote: Human BLaER1 cells (14) were kindly provided by Thomas Graf (CRG, Barcelona) and grown in RPMI medium (Invitrogen) supplemented with 10% heat-inactivated foetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2021Quote: The human episomal line of induced pluripotent stem cells (A18945, Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) was maintained in feeder-free culture on Geltrex matrix (A1413202 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human CD171 (i.e. antibody against neural cell adhesion molecule L1CAM; RRID: AB_2043813; Thermo Fisher Scientific, Waltham, MA) or anti-human tetraspanin CD81 (Ancell Corporation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and any antigen–MS-Hu6 complex was captured by goat anti–human HRP–conjugated IgG (Invitrogen, Catalog # A18805).
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bioRxiv - Microbiology 2022Quote: ... falciparum was cultured in O+ human RBCs (Interstate Blood Bank, TN) supplemented with RPMI1640 medium along with 0.3-0.5% Albumax I (Invitrogen), 2g/L sodium bicarbonate ...
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bioRxiv - Molecular Biology 2022Quote: ... Human HAP1 cells were obtained from Horizon Discovery and were grown in Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco), containing L-glutamine and 25 nM HEPES and supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... 19-805) of human ACE2 (GenBank NM_021804.1) was cloned in to the NheI-XhoI sites of pCEP4 (Invitrogen) with a N-terminal HA leader (MKTIIALSYIFCLVFA) ...
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bioRxiv - Molecular Biology 2020Quote: ... we used TaqMan probes for human HTT and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs obtained from Applied Biosystems. Relative HTT gene expression was determined using the ddCt method ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: Human ISYNA1 isoform 2 (UniProt classification) was cloned into a pcDNA4/TO mammalian expression vector (Thermo Fisher Scientific). The cDNA was amplified by PCR (forward primer ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM ...
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bioRxiv - Genomics 2020Quote: ... LECs were stained with probes designed to target human LETR1 (Type 1 Probe, VA1-3018146, Thermo Fisher Scientific), human Malat1 (positive control ...
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bioRxiv - Immunology 2021Quote: ... Alexa Fluor 647 anti-human CD137 (clone 4B4-1; dilution 1:100; Thermo Fisher Scientific; Cat no A51019); PE anti-human CD134 (clone OX40 ...
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bioRxiv - Molecular Biology 2021Quote: ... T cells were either frozen or stimulated for 2 days with anti-human CD3/CD28 magnetic Dynabeads (ThermoFisher) at a bead to cell concentration of 1:1 ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumor genomic copy number was also assessed using the Genome-Wide Human SNP array 6.0 (Thermo Fisher Scientific). DNA prehandling and array hybridization were performed according to the manufacturer’s instructions (Affymetrix ...
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bioRxiv - Physiology 2020Quote: ... RNA isolation of human left ventricular biopsies was performed with TRIzol (Invitrogen/ThermoFisher Scientific Inc., Waltham, MA, USA). The cardiac tissue samples (0.3 – 5.4 mg ...
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bioRxiv - Bioengineering 2019Quote: Human adult fibroblasts were harvested and re-suspended at 1.5×106 cells/mL in dermal proliferation media (ThermoFisher, DMEM-High Glucose supplemented with 1% Pen/Strep ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Bound cynomolgus monkey antibodies were detected with alkaline phosphate-labeled goat-anti-human IgG (1:1000, 4HI1305, Invitrogen Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... T cells were isolated using Dynabeads Untouched Human T Cells Kit using manufacturer’s protocols (Thermo Fisher, Waltham, MA). Isolated T cells were kept in T cell media ...
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bioRxiv - Genomics 2019Quote: Immortalized human astrocytes (51) were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) plus 10% fetal bovine serum (FBS; Invitrogen) on fibronectin-coated (F1141 ...
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bioRxiv - Immunology 2019Quote: ... and anti-human HLA-DR conjugated to eVolve 605 (clone LN3, cat. no. 83-9956-41, Affymetrix-Ebioscience). For bulk RNA-seq datasets ...
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bioRxiv - Microbiology 2019Quote: ... and human monocytic leukemia (THP-1) cells (TIB-202; ATCC) cell lines were maintained in RPMI medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... was analyzed using Affymetrix Human Transcriptome Array 2.0 (HTA 2.0) microarrays after cDNA amplification using GeneChip WT Pico Reagent Kit (Affymetrix) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... were co-cultured on irradiated murine embryonic fibroblasts in human embryonic stem cell medium [DMEM/F12 (ThermoFisher Scientific), 20% Knockout Serum Replacement (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... Human U2OS cells and mouse 3T3 cells were counted using the Countess II automated cell counter (Thermo Scientific) and fixed with 2% formaldehyde using the Arima Hi-C Kit (Arima) ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using a 1:1 ratio of DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher) for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... Human embryonic kidney cells (293T; ATCC; ATCC CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), 10% fetal calf serum (FCS) ...
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bioRxiv - Microbiology 2021Quote: ... Both human and simian hepatocytes were maintained at 37 °C in 5% CO2 in William’s E medium (Gibco) supplemented with 10% fetal clone III serum (FCS ...
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bioRxiv - Neuroscience 2021Quote: ... and a mouse monoclonal antibody against human KMO (# 60029-1-Ig 1:1000, Thermo Fisher Scientific, Massachusetts, EUA). The membranes were washed and incubated for 1 h at room temperature with an HRP-conjugated secondary antibody (1:10000 ...
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bioRxiv - Biochemistry 2020Quote: CD3/CD28 Dynabeads were removed from stimulated primary human CD4+ T cells using a DynaMag-2 magnet (Invitrogen). For antibody staining ...
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bioRxiv - Bioengineering 2020Quote: The human fetal osteoblast (hOB) 1.19 cell line (ATCC) was expanded at 33.5°C in DMEM/F12 media (Gibco) containing 10% FBS (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Human phospho-tau [pT181] was measured using a commercially available ELISA per manufacturer’s instructions (Thermo Fisher Scientific, KHO0631). Guanidine extracted supernatants of hippocampus homogenates described above were utilized for the pTau ELISA.
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bioRxiv - Immunology 2020Quote: ... and mouse anti-human HLA-DR antibody (Thermo Fisher Scientific, USA, 1:100, 2 h at 37°C). Also ...
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bioRxiv - Neuroscience 2020Quote: ... Total Tau, phospho Tau (pS396, pS199, pT231 and pT181) levels were analysed by Human ELISA Kit from Invitrogen. Cerebral organoids and supernatants (for Aβ ...
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bioRxiv - Cancer Biology 2021Quote: MMP1 in the secretome of cell lines was quantified with a MMP1 Human ELISA Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and then with the following secondary antibodies: goat anti-human IgG (H+L) Alexafluor 488 (Invitrogen, 1/200), goat anti-mouse IgG2b Alexafluor 647 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Fibroblasts were isolated from normal human skin and cultured in Dulbecco’s modified Eagle medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal calf serum ...