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Citations for Thermo Fisher :
5701 - 5750 of 7745 citations for Recombinant Human PPIA His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney (HEK) 293T and Vero cells were grown in Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Invitrogen).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... targeting human SLC22A10 (Assay ID: Hs01397962_m1) and beta actin (Assay ID: Hs99999903_m1) (Applied Biosystems, Foster City, CA). The qRT-PCR reactions were conducted in a 96-well plate ...
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bioRxiv - Immunology 2023Quote: The human myelogenous leukemia cell line K562 was cultured in Roswell Park Memorial Institute (RPMI) media (Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: Fibroblasts were isolated from human skin biopsies and were cultured in DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Bioengineering 2023Quote: Human foreskin fibroblasts (HFFs; CRL-2429, ATCC) and HFF-derived iPSCs were grown in Geltrex (Gibco, A1413302) coated 12-well plates and were fixed with 3.7% formaldehyde and then blocked with 3% BSA in 1X PBS for 1hr at Room Temperature prior to imaging ...
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bioRxiv - Bioengineering 2023Quote: ... The tissues were then incubated with primary mouse monoclonal anti-human cardiac troponin T (Thermo Fisher Scientific) or mouse monoclonal anti-human CD3 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: Both plasmids encoding for the LC and HC were transfected into Expi293F human cells (Thermo Fisher Scientific) with purified DNA and polyethylenimine (PEI 1:3) ...
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bioRxiv - Cancer Biology 2023Quote: ... SW837 (human colon cells, ATCC CCL-235) were grown in Leibovitz’s L-15 medium (Gibco #11415-049) with 10% FCS (Hyclone #SH30084.03n ...
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bioRxiv - Cell Biology 2023Quote: Normal human epidermal keratinocytes (Kurabo) from Caucasian neonatal foreskin were cultured with EpiLife medium (Thermo Fisher Scientific) supplemented with 60 µM CaCl2 and HuMedia KG growth factor kit (Kurabo) ...
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bioRxiv - Genomics 2022Quote: ... CD4+ T cells were isolated by magnetic negative selection using Untouched Human CD4+ Isolation Kit (Invitrogen 11346D) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was ESTEM-HE medium during purification and changed to Human Melanocyte Basal Medium Kit (Gibco) or Melanocyte Growth Basal Medium-4 (Lonza ...
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bioRxiv - Developmental Biology 2023Quote: ... Small interfering RNA was introduced into primary human umbilical vein ECs using the Neon transfection system (ThermoFisher). See Supplementary Table 5 for sources of siRNA ...
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bioRxiv - Microbiology 2023Quote: ... human actin mRNA and EMCV viral RNA was performed mixing the Fast SYBR green Master Mix (ThermoFisher) with cDNA and 1µM of the forward and reverse corresponding primers ...
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bioRxiv - Bioengineering 2023Quote: ... substrates were incubated with PBS solution containing various concentrations of human plasma fibronectin (Thermo Fisher Scientific, US) for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with HRP-coupled anti-human IgG (MilleniumScience) before adding the chromogenic substrate TMB (ThermoFisher). Reactions were stopped with 2 M H2SO4 and absorption measured at 450 nm.
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bioRxiv - Genomics 2023Quote: ... KRAB-dCas9 expressing cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific, 11131D), at 1:1 cell:bead ratio ...
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bioRxiv - Bioengineering 2023Quote: ... the brain sections were incubated for 1 h with goat anti-human IgG Alexa Fluor 647 (Invitrogen) and goat anti-mouse IgG Alexa Fluor 488 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from mouse aortas and human aortic SMCs with the PureLink RNA Mini Kit (ThermoFisher). Isolated RNA was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: Human cervix adenocarcinoma epithelial HeLa 229 cells (ATCC CCL-2.1) were cultured in RPMI 1640 medium (Gibco) containing 5% heat-inactivated fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... falciparum 3D737 infected erythrocytes were cultured in O positive human erythrocytes in RPMI 1640 media (Gibco, USA) containing 10% heat-inactivated pooled human sera and 5 μg/mL gentamicin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... version 46) and humans using SEQUEST and Mascot (Version 2.4.1) via Proteome Discoverer v2.2 (Thermo Fisher Scientific). Trypsin was selected as the protease of choice with a maximum of two missed cleavages ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 human monocytes (ATCC) were maintained in Roswell Park Memorial Institute 1640 Medium (RPMI 1640, Gibco) supplemented with 10% FBS ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from human lung ECs and mouse lung tissues using TRIZOL reagent kit (Invitrogen). The cDNA was generated using iScript cDNA synthesis kit (170-8891 ...
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bioRxiv - Immunology 2024Quote: ... 1:2000 dilution of secondary Alexa FLUOR-647 GOAT-Anti human IgG [H+L] (Invitrogen; #A-21445) was added to the cells for 30 minutes followed by 3 washes with FACS Buffer ...
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bioRxiv - Systems Biology 2024Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) media (ThermoFisher, UK), supplemented with 10% Heat Inactivated Foetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: Elastase generated granulin peptides were obtained by incubation of rPGRN with human neutrophil elastase (# RP-77526, Invitrogen). 50 ng/µl PGRN and 80 ng/µl elastase were incubated in 100 mM sodium citrate pH 6.0 overnight at 37°C ...
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bioRxiv - Pathology 2024Quote: Gene expression on human cells and tissue was quantified using TaqManTM assays (Fisher Scientific, Supplementary Table 3) and Luna® Universal qPCR Master Mix (M3003E ...
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bioRxiv - Microbiology 2023Quote: ... and 50 ng of human ACE2-encoding plasmid or an empty plasmid control using Lipofectamine 2000 (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and S-D614G-N149Q were expressed in human Expi293F cells using the Expi293 Expression System (ThermoFisher Scientific) and purified using a HisTrap FF column (GE Life Sciences)(64 ...
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bioRxiv - Microbiology 2023Quote: ... Secreted antibodies were detected with 1µg/mL goat anti-human IgG (H+L)-Alexa Fluor 594 (Invitrogen), which was added to the cell culture media used to resuspend the antigen-coated beads.
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bioRxiv - Molecular Biology 2023Quote: ... human primary T cells and DG-75 cells were electroporated using the Neon Transfection kit (ThermoFisher Scientific) at 1600 volts / 10 ms / 3 pulses ...
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bioRxiv - Neuroscience 2023Quote: Measurement of Aβ1-40 levels was performed using an Amyloid beta 40 Human ELISA Kit (Invitrogen, KHB3482). For further information please see Supplementary Material.
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bioRxiv - Immunology 2023Quote: ... A goat anti-Human IgG secondary antibody labelled with Alexa Fluor 488 was added (Thermo Fisher Scientific). The plates were incubated for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were probed with rabbit monoclonal anti-human CD38 antibody (Clone RM388, Thermo Scientific, cat# MA5-36061) after blocking with 5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... SK-N-Be(2c) and SK-N-SH human neuroblastoma cells were cultured in DMEM (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... and TOMM22) were amplified using an Ion AmpliSeq Transcriptome Human Gene Expression Core Panel (Thermo Fisher Scientific). Amplicons were ligated to barcode adapters and purified using Agencourt AMPure XP reagent (Beckman Coulter Inc.) ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary staining was done using a goat anti-Human Alexa Fluor (AF) 594 antibody (1:1000, Invitrogen), for 30 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... Human iPSC spheroids were washed and growth factor-depleted using DMEM/F12 (Thermo Fisher Scientific #21331-046) for 2 h prior to stimulation ...
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bioRxiv - Systems Biology 2024Quote: Human osteosarcoma epithelial cells (U-2 OS cell line) were cultivated in DMEM high glucose medium (Gibco), supplemented with 2 mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... at an interval of 24 hr with siRNAs targeting human PKR/EIF2AK2 (Assay ID 142330-Thermofisher scientific) or human TIA-1 (Assay ID 139893-Thermofisher scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... and adapter ligation were performed using an Ion AmpliSeq Transcriptome Human Gene Expression Kit (Thermo Fisher Scientific). The cDNA library was purified using Agencourt AMPure XP Reagent (Beckman Coulter ...
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bioRxiv - Biochemistry 2024Quote: Synchronized or treated human embryonic kidney (HEK293T) cells were lysed in RIPA buffer (Thermo Fisher Scientific # 89901) containing 10mM of sodium butyrate ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was collected on day 3 and analyzed by human IL-2 ELISA Kit (Thermo Fisher) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: Human A549 lung carcinoma cells (ATCC, CCL-185) were maintained in Gibco DMEM/F-12 (Thermofisher 11320033) with 100 U/ml penicillin ...
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bioRxiv - Developmental Biology 2024Quote: A synthetic sequence of human Twisted gastrulation (residues Cys26-Phe223) codon optimised for mammalian expression (ThermoFisher Scientific) with a C-terminal TwinStrep-tag (IBA LifeScience ...
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bioRxiv - Biophysics 2024Quote: Human mesenchymal stem cells (hMSCs) (ATCC, lgc-standards, Teddington, UK) were cultured in high-glucose DMEM (Gibco) supplemented with 10% FBS and 1 % penicillin/streptomycin ...
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bioRxiv - Biophysics 2024Quote: Human-derived neuroblastoma cells (SH-SY5Y, ATCC Product Number: CRL-2266) were cultured in DMEM-F12 (Invitrogen) medium supplemented with 10 % (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... or ON-TARGETplus Human SUSD3 SMARTPool siRNA (Dharmacon #L-016811-02-0005) using Lipofectamine RNAiMAX (Invitrogen #13778) protocol for 24 or 72 hours.
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bioRxiv - Biochemistry 2024Quote: The human derived NCH612 glioma cell media was prepared using 500 mL DMEM: F12/Glutamax media (Gibco) with the following additives ...