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Citations for Thermo Fisher :
5601 - 5650 of 10000+ citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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bioRxiv - Bioengineering 2021Quote: ... Primary human astrocytes were grown on the bottom of transwell inserts (24-well, 0.1 um pore size, PET, Fisher Scientific) which were coated with poly-L-lysine (20 ug/mL) ...
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bioRxiv - Biochemistry 2021Quote: Codon optimized human SERT was assembled from synthetic DNA fragments (IDT) and inserted into the NheI-XhoI sites of pCEP4 (Invitrogen). The use of a synthetic gene ensures there are no sequence features ...
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bioRxiv - Neuroscience 2021Quote: ... The tandem mass spectra were searched using SEQUEST 80 search algorithms against a Uniprot database (human 2019_07 release for 293T CoIP and mouse 2019_08 release supplemented with human TDP-43 protein sequence for mouse CoIP) through the Proteome Discoverer platform (version 2.5, Thermo Scientific). The search parameters included a maximum of two missed cleavages ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were generated by RUCDR Infinite Biologics® from human primary lymphocytes using Sendai viral vectors (CytoTune™, ThermoFisher Scientific), as previously described (Moore JC et al ...
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bioRxiv - Cancer Biology 2021Quote: Isolated human PBMCs were seeded and grown in 6 well culture dish with hybridoma-(SFM) serum free medium (Cat #12045076, Gibco). PBMCs were treated with patient EVs under serum free conditions for 24 hrs ...
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bioRxiv - Cell Biology 2021Quote: Human foetuses were obtained following elective termination of pregnancy with full consent and stored overnight in Hibernate-A Medium (Gibco) at 4° C ...
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bioRxiv - Cell Biology 2021Quote: MS/MS spectra were searched against a Swiss-Prot human database containing 20,341 reviewed protein entries using Mascot algorithm (Matrix Science) via Proteome Discoverer 2.2 (PD, Thermo Fisher Scientific) and were processed as previously described (10) ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Cell Biology 2021Quote: ... A probe mix containing 60 ng of each library probe and fluorescently labeled readout probe and 40 μg human COT1 DNA (Invitrogen) in 1.1 ml of hybridization buffer (20% dextran sulfate ...
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bioRxiv - Cancer Biology 2020Quote: ... NOK1 cells were grown in keratinocyte-SFM medium supplemented with human EGF and bovine pituitary extract (BPE) (Thermo Fisher Scientific) and induced to differentiate by transfer to Dulbcecco modified Eagle medium (DMEM ...
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bioRxiv - Cancer Biology 2020Quote: ... HFKs and iHFKs were grown and maintained in keratinocyte-serum-free media (KSFM) supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Invitrogen). IMR-90s normal human diploid lung fibroblasts were purchased from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2019Quote: ... human GRPR shRNA in Lentiviral Vector (Genomics Online; Limerick, PA, # ABIN3479889) and mouse Grpr siRNAs (Life Technologies, #157912 and #157914) were used for all knockdown experiments ...
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bioRxiv - Cell Biology 2021Quote: Human non-transformed retinal pigment epithelial cells transduced with the human telomerase gene (hTERT-RPE1; ATCC CRL-4000) were grown in DMEM/F12 (Gibco) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Bioengineering 2020Quote: ... 2-3 ⨯ 105 cells were collected for each condition and samples were stained with the following antibodies: APC-conjugated anti-human CD4 (Invitrogen), FITC-conjugated anti-human CD4 (BD Bioscience) ...
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bioRxiv - Biochemistry 2021Quote: The knock-out cell line (GTPBP5KO) was generated in the Flp-In T-Rex human embryonic kidney 293 (HEK293T) cell line (Invitrogen) using CRISPR/Cas9 technology targeted on exon 1 of MTG2 gene ...
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bioRxiv - Cell Biology 2021Quote: Human islet and hESC-derived differentiated organoids (approximately 20 organoids or human islets per well) were respectively plated into a 96-well black plate (ThermoFisher) coated with 1% hESC-qualified Matrigel ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Microbiology 2020Quote: ... human serum or oseltamivir were serially two-fold diluted in Dulbecco’s phosphate buffered saline with calcium and magnesium (ThermoFisher Scientific) containing 1% BSA (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... ATCC) and HeLa (a human cervical cancer cell line, ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2021Quote: ... the bound antibody was detected with goat anti-human Ig (γ chain specific)-HRP conjugated secondary antibody (Thermo Fisher Scientific) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... if sequences diverged between strains) were codon-optimised for expression in human cells and synthesised by GeneArt (Thermo Fisher Scientific), with an optimal 5’ Kozak sequence and fused to an N-terminal FLAG epitope ...
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 cells83 were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (Invitrogen, Paisley, UK) supplemented with 10% heat inactivated fetal bovine serum (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with 500ng of a subset of ACE2 expression constructs (human, hamster, rabbit, pig, chicken, horseshoe bat) or mock transfected with an empty vector (pDISPLAY) in OptiMEM (Gibco) using TransIT-X2 transfection reagent (Mirus ...
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bioRxiv - Microbiology 2021Quote: ... Caco-2 (human epithelial colorectal adenocarcinoma, kindly provided by Prof. Holger Barth, Ulm University) cells were cultivated in DMEM (Gibco) containing 10% FBS (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... followed by internalization into target cells and detection by incubation with secondary anti-human-Alexa 555 fluorophore conjugates (Thermo Scientific). Cells were examined by immunofluorescence analysis performed on an Axio Observer Z1 widefield epifluorescence microscope (Zeiss Inc. ...
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bioRxiv - Microbiology 2021Quote: ... and HCT 116 p53- /- (human, kind gift from Dr. Wilson) were grown in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplied with 10% FBS (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... HUVECs were cultured in a mixture of 199 medium and SFM (Human Endothelium Serum-Free Medium, GIBCO, cat. No. 11111044), with 10% of FBS (Biowest ...
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bioRxiv - Cancer Biology 2021Quote: Two biological replicates of RNA samples were isolated from A549 cells transduced with pMSCV-dnCRTC or pMSCV-GFP retroviruses at 72 hours post-infection and were then subjected to microarray experiment using GeneChip® Human Transcriptome Array 2.0 (Affymetrix) at the Genomics Core at Sanford Burnham Research Institute ...
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bioRxiv - Cancer Biology 2021Quote: ... Gene expression data was collected using the Gene Chip® Human Genome U133 Plus 2.0 Array (Affymetrix, Santa Clara, CA) protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... the slides were incubated with anti-human IgG1-AlexaFluor 594 or streptavidin Alexa Fluor 594 (Life Technologies-Thermo Fisher Scientific) diluted in PBS + 1% BSA buffer for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... the slides were incubated with anti-human IgG1-AlexaFluor 594 or streptavidin Alexa Fluor 594 (Life Technologies-Thermo Fisher Scientific) diluted in PBS + 1% BSA buffer for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were from A Human Glioblastoma Cell Culture Resource (HGCC) and were cultured as described (Xie et al., 2015) in Neurobasal medium (GIBCO)/DMEM/F12 with Glutamax (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... ON-TARGET plus human MGEA5 (10724) siRNA-smartpool) or nontargeting siRNA (Dharmacon, ON-TARGETplus nontargeting pool) using Lipofectamine RNAiMAX (Invitrogen) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... and ESRG_cn2 (Hs06675423_cn) detected the ESRG locus and TaqMan Copy Number Reference Assay human RNase P (4403326, Thermo Fisher Scientific) was used as an endogenous control ...
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bioRxiv - Cell Biology 2020Quote: ... and target genes were amplified for 12 cycles of PCR using the Ion AmpliSeq Human Gene Expression Core Panel (Thermofisher). This panel contains a pool of 20,802 amplicons (41,604 primers ...
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bioRxiv - Cancer Biology 2021Quote: ... and Non – metastasis human colon cancer cell line (HT-29) were cultured in Dulbecco’s modified Eagle’s medium (DMEM: Gibco, Thermo Fisher, USA) and metastasis human colon cancer cell line (SW-620 ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: Human ProtoArray® Microarray (v5.0) containing 9483 unique human proteins expressed as GST-fusion in insect cells were purchased from Invitrogen. Arrays were blocked in 4°C cold blocking buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were washed and stained for flow cytometry using appropriate seconday antibodies: goat anti-human fluorescein isothiocyanate (FITC; ThermoFisher A18812), anti-mouse Alexa Fluor 488 (Cell Signaling Technology 4408S) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and NK cells were isolated from cryopreserved PBMCs using commercial kits (MagniSort Human CD4+ T Cell Enrichment; Affymetrix, and MagniSort™ Human NK cell Enrichment ...
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bioRxiv - Biochemistry 2022Quote: ... After 3hrs from release of the nocodazole block cells were double transfected with pEGFP-human LA (EGFP-wt LA and mutants) and RFP-HP1α using Lipofectamine 2000 (Invitrogen) in accordance with the manufacturer’s protocol at early passages having 60-70% confluency and were kept in culture for 24 hrs.
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bioRxiv - Cell Biology 2022Quote: The human cDNA encoding zDHHC2 was codon optimised for protein expression in insect cells and synthesized by GeneArt technology (Invitrogen). It was inserted with N and C-terminal tags (His6 and StrepII ...
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bioRxiv - Bioengineering 2022Quote: ... immortalized human dermal fibroblast cell line (Stephens et al., 2004) were cultured in Dulbecco’s modified Eagle medium (DMEM, Gibco™) supplemented with 10% fetal bovine serum (FBS ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
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bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected using goat anti-Human IgG Fc Secondary Antibody conjugated to horse radish peroxidase (Thermo Fisher Scientific), diluted 1:5000 in blocking solution and incubated for 1 hr at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... Paraffin-embedded human tissues and hind paws were cut into 5 μm sections with the Microtome HM355S (Thermo Fisher Scientific) and transferred onto microscope slides.