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Citations for Thermo Fisher :
5401 - 5450 of 10000+ citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: A CRISPR plasmid targeting human Hk2 was generated using the GeneArt® CRISPR Nuclease Vector Kit from Thermo Fisher. Caco-2 cells were purchased from DSMZ (ACC-169 ...
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bioRxiv - Genomics 2021Quote: ... Naive and memory T cells were then stimulated with anti-CD3/anti-CD28 human T-Activator Dynabeads® (Invitrogen) at a 1:2 beads-to-cells ratio ...
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bioRxiv - Genomics 2021Quote: ... RNA for gene expression analysis from primary human pseudoislets was extracted using the PicoPure RNA isolation kit (Life Technologies) and cDNA was synthesized using the Maxima first strand cDNA synthesis kit (Thermo Scientific) ...
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bioRxiv - Genomics 2021Quote: Human iPSCs from WTC11 and NCRM5 backgrounds3 were cultured in StemFlex Medium (GIBCO/Thermo Fisher Scientific; Cat. No. A3349401) in plates or dishes coated with Growth Factor Reduced ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Genetics 2021Quote: ... were genotyped using the Affymetrix Genome-Wide Human SNP Array 5.0 (SNP5) chip (Affymetrix Inc., Santa Clara, CA, USA). For the Athero-Express Genomics Study 2 (AEGS2) ...
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bioRxiv - Genomics 2020Quote: The cells The human embryonic kidney 293T (HEK293T) cell were cultured in DMEM containing 10% FBS (Thermo Fisher Scientific), 100 U/ml penicillin (A603460 ...
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bioRxiv - Microbiology 2020Quote: ... and human umbilical vein endothelial HUV-EC-C cells (ATCC; CRL-1730) in RPMI 1640 medium (Gibco; #11875-093) containing 10% FBS ...
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bioRxiv - Molecular Biology 2021Quote: The coding sequence of MHV-S Hemagglutinin Esterase inactive (S45A) (1218bp) fused to human IgG-Fc followed by a 6xHis tag in the mammalian expression vector pcDNA3.1(-) (Invitrogen) was kindly provided by Colin Parrish (MHV-S-HE_Fc_His6 ...
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bioRxiv - Developmental Biology 2022Quote: Human embryonic genital ridge or mesonephros from a week 7.0 male embryo were collected in dissection medium (DMEM (Gibco), 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... When HIV was reactivated using Dynabeads Human T activator CD3/CD28 for T cell expansion and activation (ThermoFisher Scientific), beads were magnetically removed the next day ...
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bioRxiv - Cell Biology 2022Quote: ... human embryonic kidney 293T cells (HEK; CRL-3216; ATCC; RRID: CVCL_0063) were cultured in DMEM media (12430062; Gibco™) with 10% FBS (098-150 ...
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bioRxiv - Biophysics 2022Quote: ... Secondary antibodies [Alexa 647-goat anti-mouse or Alexa 647 Goat anti-Human IgG (H+L) Cross-Adsorbed (Invitrogen)] at 1:1000 dilution were then incubated for 60 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: Human HEK-293T adherent cells were seeded at 37°C with 5% CO2 in DMEM + GlutaMAX™ (Gibco, Germany) containing 10% heat inactivated fetal calf serum (dFCS ...
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bioRxiv - Microbiology 2022Quote: ... with 1.3 µg monoclonal antibody detecting the Twin-Strep-tag and 0.325 µg goat anti-human Alexa Fluor 488 (A11013, Invitrogen). Biotinylated lectins were precomplexed (on ice ...
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bioRxiv - Molecular Biology 2022Quote: CHO-K1 cells stably expressing human RXFP4 (hRXFP4-CHO) or RXFP3 (hRXFP3-CHO) were maintained in DMEM/F12 (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2022Quote: Fresh-frozen human postmortem brain tissues were sectioned at 16-25 μm onto Superfrost Plus glass slides (Fisher Scientific). Sections were dried for 20 minutes on dry ice and then vacuum sealed and stored at −80°C until use ...
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bioRxiv - Molecular Biology 2023Quote: ... The MS/MS spectra were searched against the target-decoy Human RefSeq database (release Jan. 21, 2020, containing 80,872 entries) in Proteome Discoverer 2.1 interface (Thermo Fisher) with Mascot algorithm (Mascot 2.4 ...
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bioRxiv - Microbiology 2023Quote: ... and cytokine concentrations were quantified with the Human Cytokine 16-Plex Protein Standard (Thermo Fisher Scientific, Waltham, MA, USA). For quantification of BPI in human sera ...
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bioRxiv - Microbiology 2022Quote: ... acquired MS/MS spectra were searched against a human uniprot protein database in Proteome Discoverer 3.2 (Thermo Fisher Scientific) using Label free Quantification with Minora feature detection ...
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bioRxiv - Neuroscience 2022Quote: ... conditioned neuronal media was diluted and handled according to the protocol of the Human Aβ42 Ultrasensitive ELISA Kit (Invitrogen) followed by colorimetric readout at 450 nm.
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bioRxiv - Molecular Biology 2022Quote: Human AAA/ control tissue samples were mounted on poly-L-lysine pre-coated SuperFrost Plus slides (Thermo Fisher Scientific). Human OCT-embedded frozen tissue was cut into 8μm thick slides ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Cell Biology 2022Quote: ... Transferrin (human)-Alexa Fluor 594 (T13343) and Wheat Germ Agglutinin (WGA)-Alexa Fluor 594 (W11262) were obtained from ThermoFisher Scientific (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... followed by washing and addition of 100 µL/well of goat anti-human IgG-HRP conjugate (Invitrogen, 62-8420) at 1:500 dilution ...
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bioRxiv - Biochemistry 2024Quote: ... The human Dicer/TRBP enzyme complex was pre-mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM HEPES pH = 7.5 ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was analyzed for gene expression changes using a microarray analysis (Clariom S Human HT, Thermo Fisher Scientific). Each replicate (100ng of total RNA ...
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bioRxiv - Biophysics 2024Quote: The human melanoma cell line MV3 [32] was cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, 11504496) containing L-Glutamine and Sodium Pyruvate ...
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bioRxiv - Cell Biology 2024Quote: IMR90 fibroblasts were differentiated to myofibroblasts with 5ng/mL Recombinant Human Transforming Growth Factor -β1 (TGF-β1) (Invitrogen, #PHG9204) for 48h in serum free media ...
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bioRxiv - Cancer Biology 2024Quote: ... plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS (GIBCO, 14080-055) for 1h in 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... The levels of soluble and insoluble human Aβ40 and Aβ42 in these extracts were quantified using ELISA kits (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... falciparum erythrocytic stages were cultured in human erythrocytes (National Blood Transfusion Service, UK) and RPMI 1640 medium (Life Technologies) supplemented with 0.5% Albumax type II (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... falciparum (3D7, Dd2, and HB3) parasites were cultured in 3-5% human O+ RBCs in RPMI-1640 (Gibco, 110875093) complete medium containing 0.5% Albumax-II (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... Precomplexation of HAs was performed with 1.3 µg monoclonal antibody detecting the Twin-Strep-tag and 0.325 µg goat anti-human Alexa Fluor 488 (A11013, Invitrogen) and incubated on ice for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in keratinocyte serum-free medium supplemented with 0.05 mg/ml bovine pituitary extract and 5 ng/ml human recombinant epidermal growth factor (Gibco). T24 human bladder cancer cells were cultured in HAM’s F12 medium supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin/glutamine (PSG) ...
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bioRxiv - Genetics 2023Quote: ... 500 ng of PCR products were saturated with 2 μg of unlabeled Cot1 human DNA (Invitrogen, cat. 15279-011) and 0.2 nmol of oligonucleotides corresponding to the universal Illumina adaptors (see above ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (ThermoFisher Scientific, Waltham, MA, USA) (S12+ media ...
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bioRxiv - Genomics 2023Quote: ... and then at time 0 media was exchanged for complete HaCat media + 20ng/mL human recombinant EGF (Gibco PHG0311L) for all wells except the 0hr timepoint ...
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bioRxiv - Cancer Biology 2024Quote: ... human mammary fibroblast (MF) and cancer associated fibroblast (CAF) were maintained in DMEM/F-12 (Thermo Fisher Scientific, #21331046) supplemented with 2 mM Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µg/mL)-coated 6-well plates in hECSR medium (Human Endothelial SFM, Thermo Fisher, cat# 11111-044; B-27 Supplement (50×), Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... C2C12 myotubes and biopsy- derived human primary myotubes were transfected with plasmids using lipofectamine 3000 (Thermofisher, Waltham, MA, USA) following the supplier’s instructions.
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney 293T (HEK293T) cells were grown in complete DMEM (Dulbecco’s Modified Eagle’s Medium (DMEM, Invitrogen, Carlsbad, CA) containing 5% fetal bovine serum (Corning) ...
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bioRxiv - Neuroscience 2023Quote: ... and Gentamycin 40µg/mL and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Immunology 2022Quote: ... at 10ng/ml final concentration along with dynabeads at a ratio of 25µl Human anti CD3/28 labelled Dynabeads/10 million cells (#11132D, Gibco).
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bioRxiv - Genomics 2023Quote: ... cDNAs from the human KZFPs were codon-optimized and synthesized using the GeneArt service from ThermoFisher (former Life Technologies). Sequences were cloned into the doxycycline inducible expression vector pTRE-3HA which yields a C-terminally tagged proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... Human dispersed islets were seeded at a density of 5×104 cells/cm2 on ultra-low attachment plates (ThermoFIsher) and allowed to form spontaneous pseudoislets ...
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bioRxiv - Microbiology 2023Quote: Human telomerase immortalized fibroblasts (MRC5-hT) and HEK 293T cells were cultured in Dulbecco’s Modified Eagle Media (DMEM; Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: Human tissue blocks (N = 6) were stored at −80 °C and transferred to the cryostat (CryoStar NX70, Thermo Scientific) on dry ice ...
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bioRxiv - Bioengineering 2023Quote: ... The human dermal fibroblasts were cultured in a reconstituted Complete medium composed of DMEM medium (Dulbecco’s Modified Eagle Medium Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... for activated cells with human PLK1-specific siRNAs (#4390824, s448) and negative control siRNA (#4390846) (Invitrogen, Waltham, Massachusetts, USA) (150 ng/106 cells) ...