Labshake search
Citations for Thermo Fisher :
5551 - 5600 of 10000+ citations for 6 AMINO 4 METHYL INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2023Quote: ... Reactions were performed in a QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific), using SYBR Green chemistry (Applied Biosystems) ...
-
bioRxiv - Microbiology 2023Quote: ... Quantification was performed in triplicate using the QuantStudio 6 Pro qPCR machine (ThermoFisher Scientific). Rpl19 was used as the endogenous control for relative quantification.
-
bioRxiv - Immunology 2023Quote: Amplifications were performed with a QuantStudio 6 Pro Real-Time PCR System (Applied Biosystems). The assay lower LOD was 50 copies per reaction.
-
bioRxiv - Cancer Biology 2024Quote: ... 1.25*105 cells/well were seeded in 6-well plates (Thermo Scientific cat# 140675) and for mitochondrial enrichment ...
-
bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from duplicate wells of 6-well plates using TRIzol (Invitrogen) according to the manufacturer’s instructions and quantified using a BioAnalyzer (Agilent) ...
-
bioRxiv - Bioengineering 2024Quote: ... HEK293T cells were grown in a 6-well culture plate (ThermoFisher, cat. no. 140675) in DMEM with 10 % FBS (Life Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were run on a QuantStudio 6 Flex 384-well qPCR apparatus (Applied Biosystems). Each target gene was normalized to HPRT ...
-
bioRxiv - Cancer Biology 2024Quote: ... MSCs were used up to passage 6 and harvested by incubating with TrypLE (Gibco) to gently detach the cells from the culture surface.
-
bioRxiv - Cancer Biology 2024Quote: ... sgRNA-infected cells cultured in 6-well plates were washed by DPBS (Gibco, #C14190500BT), followed by detachment using accutase (BioLegend ...
-
bioRxiv - Molecular Biology 2024Quote: ... RNA was suspended in urea Dye and resolved on a 6% TBU gel (Invitrogen) at 200 V for 5 min ...
-
bioRxiv - Immunology 2024Quote: ... Real-time PCR was performed in a QuantStudio 6 Flex PCR System (Applied Biosystems) using the Fast SYBR Green Master Mix (Applied Biosystems) ...
-
bioRxiv - Neuroscience 2024Quote: ... on a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific, 4485689). A table with qPCR primer design is included (Supplementary Table 7).
-
bioRxiv - Neuroscience 2024Quote: HeLa cells were plated in DMEM (Cellgro, #10-013CV) with 6% FBS (Gibco, #26140) at 10,000 cells per well in 96-well tissue culture-treated plates ...
-
bioRxiv - Microbiology 2024Quote: ... the proteins were first treated with 6× Laemmli SDS sample reducing dye (Thermo scientific) and boiled for 5 min at 100 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... IMR90 PD 34 to PD 40 were seeded on 6 well plates (ThermoFisher, 140675) as the beginning points for the assays ...
-
bioRxiv - Cancer Biology 2024Quote: ... All qPCR was run on QuantStudio 6 flex real-time PCR system (Applied Biosystems). Relative abundance was calculated with delta-delta-Ct method ...
-
bioRxiv - Cell Biology 2024Quote: ... C2C12 cells on 6-well plates were transiently transfected with RNAiMAX (Invitrogen, 13778-150) according to the manufacturer’s directions in two stages to prevent over-confluency at the time of cellular treatment and lysis ...
-
bioRxiv - Genetics 2021Quote: ... and cytocentrifuged on clean slides (using a Cytospin™ 4 Cytocentrifuge, Thermo Fisher Scientific, at 900 rpm for 4 min). Slides were then immersed in liquid nitrogen for 5 min ...
-
bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
-
bioRxiv - Immunology 2021Quote: ... The supernatant was then nutated overnight at 4 °C with 1 ml of Protein G Sepharose 4 Fast Flow slurry (Invitrogen) per 25ml of supernatant ...
-
bioRxiv - Developmental Biology 2019Quote: Mouse tissues were fixed in 4% paraformaldehyde at 4°C overnight and embedded in paraffin or Sandon Cryomatrix (Thermo Scientific). Standard histology techniques were used ...
-
bioRxiv - Physiology 2019Quote: ... 20-30 μ g of protein lysates were denatured in NuPAGE 4× LDS sample buffer and resolved on NuPage 4-12 % Bis-Tris gels (Invitrogen) and the proteins transferred by iBlot (Invitrogen ...
-
bioRxiv - Neuroscience 2020Quote: ... free-floating coronal hippocampal slices of 50 µm thickness were produced and incubated overnight in staining solution (4% normal goat serum, 0.4% Triton-X 100 and 4% BSA) with anti-GFAP antibody (1:1000, ThermoFisher, rat) and anti-Iba1 antibody (1:1000 ...
-
bioRxiv - Cell Biology 2021Quote: Handpicked islets (~180) from control (n=4) and diabetic (n=4) mice were placed into 1 mL Trizol (Cat# 15596018, Ambion) and processed for RNA extraction ...
-
bioRxiv - Microbiology 2021Quote: ... for 20 min at 4 °C and were then centrifuged at 444g for 4 min and dissociated to single cells using StemPro Accutase (Invitrogen). Cells were subsequently fixed using 4% paraformaldehyde (PFA ...
-
bioRxiv - Bioengineering 2020Quote: ... and media for M(IL4/IL13) stimulation consisted of RPMI 1640 with 20 ng/ml interleukin 4 (IL-4; Invitrogen) and 20 ng/ml interleukin 13 (IL-13 ...
-
bioRxiv - Biochemistry 2021Quote: ... 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, #D22065) and 6,8-difluoro-7-hydroxy-4-methylcoumarin (DiFMU, #D6566) were purchased from Life Technologies.
-
bioRxiv - Biochemistry 2020Quote: ... The isolated fractions were separated on SDS-PAGE (RunBlue 4-20 %, Expedeon; NuPAGE®Bis-Tris gel 4-12%, Invitrogen) and analysed by InstantBlue staining (Expedeon ...
-
bioRxiv - Biochemistry 2021Quote: ... gondii parasites were separated by polyacrylamide gel electrophoresis (PAGE) in precast NuPAGE (4-12% or 12%) or NativePAGE (4-16%) gels (ThermoFisher) according to the manufacturer’s instructions with minor modifications (detailed in the SI) ...
-
bioRxiv - Microbiology 2021Quote: ... loaded equivalently in each lane (ranging from 4-20 μg between experiments) and run on a 4-20% Tris-Glycine gel (Novex, Invitrogen) under reducing conditions ...
-
bioRxiv - Biophysics 2020Quote: ... The samples were incubated for 20 min at 4°C before they were loaded onto a pre-run 4-20% Novex TBE gel (Invitrogen). Electrophoresis was performed at 200 V for 45 min in TBE buffer (89 mM Tris ...
-
bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (KCL-22, K562, REH, NB-4) or 20% (MOLT-4, OCI-AML3, MOLM-13, Kasumi-1) fetal bovine serum (Gibco) and 1% penicillin–streptomycin (Gibco ...
-
bioRxiv - Plant Biology 2019Quote: ... 4 µg or less (for samples with <4 µg) of total RNA was reverse transcribed using random hexamers (Thermo scientific), 200 units of Revertaid Reverse Transcriptase (Thermo Scientific) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 4 μg of a plasmid mixture (1:1:4 THR:RXR:TRE-Luc) in 200 μL Opti-MEM (Invitrogen, 11058-021) and added to the cells ...
-
bioRxiv - Molecular Biology 2020Quote: ... Six hundred μg of the supernatant were incubated for 4 hours under rotation at 4°C with 20 μl of protein A Dynabeads (Invitrogen) containing anti-UNR antibody or rabbit IgG as control in a final volume of 600 μl ...
-
bioRxiv - Plant Biology 2021Quote: ... GUS activity was determined by measuring the fluorescence (excitation wavelength 365 nm and emission wavelength 460 nm) of produced 4-Methylumbelliferon (4-MU) in a 96well polystyrene microwell plate (Nunc F96 black flat bottom, ThermoFisher) by the Tecan Infinite 200 PRO microplate reader ...
-
bioRxiv - Immunology 2020Quote: ... 4-incubation with 4 μg/mL secondary goat anti rabbit conjugated with AlexaFluor(AF)488 (goat IgG H+L, Invitrogen) in microscopy buffer for 1 h at RT ...
-
bioRxiv - Cell Biology 2019Quote: Total RNA was extracted from DCMA fibroblasts (n=4) and control fibroblasts (n=4) using the RNA extraction Mini kit (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2019Quote: ... 150 ng of total RNA extracted from the brainstem of Vglut2:Ndufs4cKO (n=4) and Vglut2:Ndufs4cCT mice (n=4) was amplified and biotin-labeled using the Illumina TotalPrep RNA Amplification kit (Ambion). 750 ng of the labeled cRNA was hybridized to MouseRef-8 v2 expression beadchips (Illumina ...
-
bioRxiv - Developmental Biology 2021Quote: ... Stained lenses were washed three times in 4°C Medium 199 and then 200 μL 4°C PBS (Gibco, MA) was added to each well prior to measuring DHR fluorescence intensity at Ex/Em of 507/529 nm and Hoescht 33342 at Ex/Em of 360/460 nm with a microplate reader (Spectramax M3 ...
-
bioRxiv - Cell Biology 2019Quote: ... Equal amounts of proteins were loaded on either a 4-12% Bis-Tris gel or a 4-20% Tris-Glycine gel (Invitrogen), and SDS gel electrophoresis was performed as previously described (96 ...
-
bioRxiv - Neuroscience 2022Quote: Brains were post-fixed overnight at 4’C in 4% PFA then cryoprotected in 30% (w/v) sucrose (Fisher Scientific) solution in PBS for at least 48 hours prior to processing ...
-
bioRxiv - Immunology 2022Quote: ... and 125) with TSt-4 (B cells) or TSt-4/DLL1 stromal cells (T cells) in RPMI (Thermo Fisher Scientific) supplemented with 10% BSA (093001 ...
-
bioRxiv - Biochemistry 2022Quote: ... The complex was eluted by rotating the tubes for 2 hours at 4 °C in elution buffer containing 4 mM SDA (NHS-diazirine, succinimidyl 4,4′-azipentanoate, Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... in 500 μL HEPES (25 mM)/PBS were mixed overnight at 4 °C with 5 μL of 4 μm aldehyde-activated latex beads (A37304, Invitrogen). The beads were blocked with 200 μL of 1% BSA for 1 h at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
-
bioRxiv - Neuroscience 2022Quote: ... and the samples were first postfixed overnight in 4 % PFA (4°C) and then placed in 0.25 M EDTA (Invitrogen AM9261) in PBS at 4°C for 3 d for decalcification ...
-
bioRxiv - Systems Biology 2022Quote: ... Proteins were then denatured by incubating samples at 95°C for 5 min in 4 x Laemmli buffer and dithiothreitol (DTT) before loading onto 4 to 12% NuPAGE gradient precast gels (ThermoFisher). Gels were run for 10 min at 110 V ...
-
bioRxiv - Neuroscience 2022Quote: ... 4% 2-Mercaptoethanol and Bromophenol Blue) and loaded on NuPAGE 4-12% Bis-Tris Gel (catalog #WG1402BOX; Thermo Fisher Scientific). Proteins were transferred on PVDF membrane (catalog #162-0177 ...
-
bioRxiv - Microbiology 2024Quote: ... at physiological pH before being spun down and resuspended in PBS with the addition of FM 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide (Cat. no. T3163, Invitrogen). Images were taken on a Zeiss X10 light microscope and cell area was measured using CellProfiler 4.2.1 (33,34).