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Citations for Thermo Fisher :
5351 - 5400 of 10000+ citations for 6 AMINO 4 METHYL INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Protein samples (4×106 cell equivalents per lane) were separated on 4%-12% Bis-Tris gels (Thermo Fisher Scientific) and then transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... or BA.4/5 spike protein (4 μg/mL) was immobilized on 96-well Maxisorp ELISA plates (Thermo Fisher) overnight at 4°C in coating buffer (1X PBS supplemented with 0.05% Tween-20 ...
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bioRxiv - Biophysics 2024Quote: ... 4 µl of cell suspension were applied and plunge-frozen using a Vitrobot Mark 4 system (Thermo Fisher Scientific) at blot force 10 ...
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bioRxiv - Plant Biology 2024Quote: ... for 6h at 4°C and an additional 2h at 4°C with 20μl Dynabeads Protein G (#10003D, ThermoFisher) blocked with 0.1% BSA ...
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bioRxiv - Bioengineering 2023Quote: B-RBD cells (1 × 107 cells/mL) were combined with 4 μM Fluo-4 AM (Invitrogen, USA, Cat: #F14217) for 20 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... were treated either with 0.8 μM CF-4 or control CF-4 (Ctrl-S2-CF4) in DMEM without phenol red (Gibco) and incubated for 10 min at 37°C and 5% CO2 ...
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bioRxiv - Biophysics 2023Quote: ... The protein was incubated overnight at 4°C with stirring with 4× molar excess AL594 or AL647 maleimide (Invitrogen). The labeled protein was concentrated and buffer exchanged into 20 mM Tris pH 7.4 ...
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bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
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bioRxiv - Immunology 2023Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Bioengineering 2023Quote: ... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) was purchased from ThermoFisher Scientific.
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bioRxiv - Developmental Biology 2024Quote: ... Cells were spun down at 1,000xg for 4 min and fixed with 4% PFA (Thermo Fisher, Cat. No. 28908) for 15 min on ice ...
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bioRxiv - Bioengineering 2024Quote: Live and dead cells were stained with 1.6-4 mM calcein AM (C125400; TRC) and 4 mM ethidium homodimer-1 (EthD-1 L3224; ThermoFisher), respectively ...
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bioRxiv - Biophysics 2021Quote: ... were grown in proliferation medium (DMEM-F12 GlutaMAX™ + 10% heat inactivated FBS + 1% non-essential amino acids +10 mM HEPES buffer + 1% Penicillin/Streptomycin; Gibco®, Invitrogen, USA). For microinjection the cells were seeded on glass bottom µ-Dishes with a cell location grid (grid-50 ...
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bioRxiv - Cancer Biology 2022Quote: ... The SKBR3 cell-membrane proteins were isolated by either the biotinylation of amino (EZ-Link Sulfo-NHS-SS-Biotin, Thermo Fisher Scientific, Rockford, IL) or oxidized glycan (EZ-Link Alkoxamine-PEG4-Biotin ...
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bioRxiv - Cell Biology 2019Quote: ... Matrigel was covalently attached to the polyacrylamide gels and to amino-silane coated glass with the bifunctional cross-linker Sulfo-SANPAH (Thermo Fisher Scientific, Waltham, USA), respectively ...
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bioRxiv - Cancer Biology 2020Quote: Chromatographic separation was performed using an Intrada Amino Acid column (150mm * 1mm, 3 μm) (Imtact Corp., Japan) on an Ultimate 3000 LC system (Thermo Fisher Scientific, Bremen, Germany). 5 μl of amino acid extract was injected and separated by binary gradient elution ...
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bioRxiv - Neuroscience 2022Quote: ... the published amino acid sequences (WO 2017/029299 AI) were used and the V-Genes were ordered as GeneArt DNA fragments (Thermo Fisher Scientific, Schwerte, Germany) and were subcloned into IgG vectors human IgG1 format by subcloning of VH in the vector pCSEH1c (heavy chain ...
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bioRxiv - Neuroscience 2021Quote: ... Dead cells were excluded from analysis by staining with LIVE/DEAD Fixable Dead Cell Stain (various amino reactive dyes; Molecular Probes, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... were grown in high glucose DMEM with non-essential amino acids supplemented with 10% (v/v) foetal bovine serum (Gibco, ThermoFisher Scientific, Waltham, MA), 1% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... and the incorporated dUTPs were labeled with amino-reactive Alexa Fluor 647 dye by using the Ares DNA labeling kit (Molecular Probes, Eugene, OR, USA) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... All tumor cell line was cultured in DMEM (30-2002, ATCC, Manassas, VA) supplemented with 100X non-essential amino acid (11140050, Gibco, ThermoFisher Scientific, Waltham, MA), 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Microbiology 2023Quote: ... and Omicron’s mutations and ΔC19 deletion (with 19 amino acids deleted from the C terminus) were cloned into pcDNA4TO (Thermo Fisher Scientific, Waltham. MA, USA)) (1) ...
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bioRxiv - Microbiology 2023Quote: ... human gastric carcinoma cell line) were routinely cultured in RPMI 1640 medium buffered with 20 mM Hepes and GlutaMAX stable amino acids (Gibco, Thermo Fisher Scientific, USA). Medium was supplemented with 10% FCS (PromoCell ...
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bioRxiv - Physiology 2024Quote: ... and sepiapterin (10 µmol/L) for 16 hours before the addition of heavy labelled amino acid DMEM medium (SILAC) (Cat. # 88364; Thermo Fisher Scientific, Rockford, USA) containing 5% FBS and sepiapterin (10 µmol/L ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were run on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher), and data were analyzed using the comparative CT method to generate expression fold-change values ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were transiently transfected in 6-well plates with Lipofectamine 2000 (Life Technologies), as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 6-ketocholestanol or phloretin cells were incubated with di-8-ANEPPS (Thermo Fisher, D3167) at a final concentration of 2 μM on ice for 20 minutes (33 ...
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bioRxiv - Cell Biology 2020Quote: ... differentiation was initiated on day -6 by adding RPMI/B27 minus insulin (Gibco, USA) supplemented with 1 μM CHIR 98014 (Selleckchem ...
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bioRxiv - Microbiology 2021Quote: ... loaded on to a Novex Pre-cast 6% TBE-Urea (8M) polyacrylamide gel (Thermofisher) in 1X TBE and run for 45-60 min ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was suspended in urea Dye and resolved on 6% TBU gel (ThermoFisher Scientific) at 200 V for 5 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected and diluted 1:6 for BCA protein quantification (ThermoFisher #23227) and performed according to manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2020Quote: ... Lower jaw timelapse experiments were carried out on 6-well transwell membranes (Nunc, 140642) with 2 ml of DMEM ...
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bioRxiv - Neuroscience 2021Quote: ... IL-6 ELISA assay was performed on collected media (Affymetrix eBioscience, #88-7066-22).
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed using QuantiStudio 6 (Life Tech) with SYBR Power Green (Applied Biosystems). All the primer sequences used in this study can be found in Table S1.
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bioRxiv - Molecular Biology 2021Quote: ... re-suspended in 6 μl water and quantified using Quant-IT RiboGreen (ThermoFisher, R11490). 1 μg RNA was resolved per lane on 1.2% glyoxal agarose gels ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 or 24-well dishes were transfected with siRNA using Lipofectamine RNAiMax (Life Technologies) following the manufacturers’ guidelines ...
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bioRxiv - Cell Biology 2022Quote: Cell transfections were performed in 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... buffer at pH 6 and two jars with nuclease-free water (ThermoFisher Scientific, USA). The slide rack was fully immersed in hematoxylin for 6 minutes and then washed by dipping the slide rack in a nuclease-free water jar 5 times following another destaining wash by dipping the slide rack in 800mL nuclease-free water for 30 times ...
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bioRxiv - Genomics 2020Quote: ... DNA-protein complexes were separated on non-denaturing 6% DNA retardation gels (Invitrogen, #EC63655BOX) at 100V constant voltage for 50 min ...
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bioRxiv - Immunology 2021Quote: ... on a QuantStudio Flex 6 Real-Time PCR System (Applied Biosystems; Foster City, CA). The primers and probe were specific to the SARS-CoV-2 nucleocapsid gene ...
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bioRxiv - Genetics 2019Quote: Genotype information was available for 21,001 NTR participants from 6 different genotyping arrays (Affymetrix 6.0 [N = 8,640] ...
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bioRxiv - Molecular Biology 2021Quote: NALM-6 cells were obtained from ATCC and cultured in RPMI medium (Life Technologies) with 10% foetal bovine serum (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Transfections were carried out in 6-well Nunclon© plates (Thermo Fisher Scientific, UK).
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bioRxiv - Evolutionary Biology 2019Quote: ... Thermal melt analysis was performed in a QuantStudio 6 Flex RT-PCR device (ThermoFisher). The melt ranged from 25°C to 98°C with 1°C change per minute and with a 2 minute incubation at the first and last temperatures ...
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bioRxiv - Microbiology 2019Quote: ... day 6 and day 9 postinoculation using the Accumet™ AE150 apparatus (Fisher Scientific). The probe was rinsed thoroughly with distilled water and then immersed in the lower chamber until the device displayed the value on the reading screen ...
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bioRxiv - Cell Biology 2019Quote: ... The myocardial sections (6 μm) were stained with Alexa Fluor 568-conjuated phalloidin (Invitrogen) to reveal F-actin and identify cardiomyocytes ...
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bioRxiv - Microbiology 2019Quote: ... 6×His-Lsr2 was purified by binding to 1 mL Ni-NTA agarose (Invitrogen), after which the resin was collected and the bound protein was washed with binding buffer supplemented with increasing concentrations of imidazole ...
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bioRxiv - Developmental Biology 2020Quote: ... Analysis of qPCR samples was performed on a QuantStudio 6 Flex system (Life Technologies).
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bioRxiv - Cell Biology 2020Quote: ... and size exclusion chromatography (Superose 6 Increase 10/300, Fisher Scientific, 45-003-210) with gel filtration buffer (50 mM NaPi ...