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Citations for Thermo Fisher :
5501 - 5550 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Brains were probed overnight at 4°C using the following primary antibody dilutions: rabbit anti-GFP (1:1000, Invitrogen #A11122), and mouse anti-brp (1:50 ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then rinsed three times in PBS (0.01M) and incubated for 2h at RT with secondary antibodies: Alexa-546 donkey anti-rabbit (1:500, ThermoFisher, #A10040), Alexa-488 goat anti-chicken (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for 1 h at RT with Cy5-conjugated goat anti-rabbit antibody (1:250, #A10523, Thermo Fisher Scientific) diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... secondary antibodies were donkey anti-rabbit Alexa Fluor 594 and donkey anti-goat Alexa Fluor 488 (Life Technologies, Victoria, Australia) diluted in Dako REAL Antibody Diluent and incubated in the dark at room temperature for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... the coverslips were incubated with 1:1000 anti-rabbit or mouse secondary antibodies coupled to Alexa Fluor 488 or 568 (Invitrogen), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were then washed 4 x 2 h in PBT and incubated overnight at 4°C in PBT supplemented with goat anti-mouse and goat anti-rabbit secondary antibodies coupled to Alexa Fluor 488 and 568 (Invitrogen). Embryos were then washed 4 x 2 h in PBT and gradually dehydrated in methanol ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 1h in goat-anti-rabbit AlexaFluor 488 secondary antibody (1:200; #A11034, ThermoFisher Scientific, MA), followed by 5x PBS washes ...
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bioRxiv - Neuroscience 2021Quote: ... SCs were then washed in PBS and incubated for 2 h at RT with secondary fluorescent antibodies (goat anti-rabbit-conjugated 649; donkey anti-guinea pig-conjugated Alexa Fluor 405 [1:1000, ThermoFisher]) diluted in 0.2% Triton X-100 blocking solution ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated in primary antibody (rabbit-anti-p65/RELA; 2 ug/mL; ThermoFisher, Waltham, MA, #10745-1-AP) diluted in blocking solution for 1h ...
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bioRxiv - Immunology 2021Quote: ... or with an antibody against the nicotinic acetylcholine receptor ɑ7 (nAChr ɑ7; Alomone Labs) followed by an Alexa594 goat-anti-rabbit conjugate (Invitrogen). Nuclear counterstain with Hoechst 33342 (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... primary antibody for 16 h at 4 °C and detected with anti-rabbit-Alexa488 (1:200, A11008, Invitrogen, CA, USA) secondary antibodies for 40 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... Blots were then washed extensively and incubated with HRP-conjugated goat anti-mouse or anti-rabbit IgG secondary antibodies (Invitrogen). Blots were developed using the SuperSignal West Pico PLUS chemiluminescent substrate kit (Thermo ...
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bioRxiv - Microbiology 2021Quote: ... incubated with corresponding secondary antibodies (Alexa488- and 594-conjugated anti-rabbit IgG, anti-mouse IgG, or anti-mouse IgG1 (1:500; Invitrogen)) in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... incubated with corresponding secondary antibodies (Alexa 488-/ 594-conjugated anti-rabbit IgG, anti-mouse IgG, or anti-mouse IgG1 (1:500; Invitrogen)) in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Prior to incubation with the secondary antibody (Alexa Fluor 488 goat anti-rabbit 1:200 dilution [A-11008] Life Technologies, Carlsbad ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... and rabbit anti-Calnexin (Stressgene, #SPA-860) followed by incubation with horseradish peroxidase-conjugated secondary antibodies and ECL Plus substrate (ThermoFisher, Pierce ECL Plus Western Blotting Substrates) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mg of pre-cleared HeLa nuclear extracts were incubated overnight at 4°C with either 40 μg ATRX antibody or rabbit IgG cross-linked to Dynabeads Protein G (Invitrogen). Beads were washed three times with 5 ml wash buffer (20 mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was washed 4 times with 1X TBS containing 0.1% (v/v) Tween-20 and incubated with 1/5,000 diluted goat anti-rabbit horseradish peroxidase-conjugated secondary antibody (Thermo Scientific) in 5% milk TBS for 1 hour at room temperature with shaking ...
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bioRxiv - Pathology 2021Quote: ... followed by incubation with secondary antibodies (Alexa Fluor® Plus 488-conjugated donkey anti-rabbit IgG (A32795, Invitrogen, 1:1000) and 594-conjugated streptavidin (U0291 ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies specific to the species of the primary antibody conjugated to horseradish peroxidase were added to the membranes: anti-rabbit (Invitrogen) and anti-mouse (EMD Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS containing 0.3 % Triton X-100 and incubated overnight at 4°C with a rabbit anti-Iba1 antibody (1:500, Wako) in PBS containing 1 % FBS (Gibco), 0.1 % BSA (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... and secondary antibodies (anti-rabbit Alexa Fluor 594, Thermo Fisher Scientific A-11037; anti-goat Alexa Fluor 594, Thermo Fisher Scientific A-11058 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were washed with PBS and incubated in donkey anti-rabbit IgG secondary antibody conjugated with Alexa Fluor 568 (1:250, A10042, Invitrogen) for 1,5 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... was used as primary antibody and goat anti-rabbit-Alexa 488 (#A11034, Thermo Fisher Scientific. Life Technologies. Massachusetts, the U.S.) as the secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... Coverslips were then washed 3 times with PBS-saponin and stained with anti-mouse Alexa Fluor 488 and anti-rabbit Alexa Fluor 568-conjugated secondary antibodies (Invitrogen), and Hoechst for 1 hour at RT ...
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bioRxiv - Microbiology 2020Quote: ... After three washes with PBS cells were incubated with the secondary antibody 1:200 (Alexa Fluor 488, goat anti-rabbit IgG, Invitrogen) for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... followed by a 2 hour incubation period at room temperature with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen]; 1:200 Alexa 488 anti-chicken [Invitrogen]) made in PBST-NGS ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then washed in 0.1% Tween/PBS and incubated with a donkey anti-mouse or anti-rabbit HRP coupled antibody (Life Technologies) for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... cells were thoroughly washed and incubated for 40 min with an anti-rabbit Alexa-Fluor 488-conjugated secondary antibody (Invitrogen). Cells were then carefully washed in PBS 1X and labeled with HCS Cell Mask TM Blue Stain (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... cells were thoroughly washed and incubated for 40 min with an anti-rabbit Alexa-Fluor 633-conjugated secondary antibody (Invitrogen), an anti-human Alexa-Fluor 488-conjugated secondary antibody (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa-Fluor 647–conjugated secondary antibodies (anti-rabbit, A21244, 1834794; anti-rat, A21247, 1719171; anti-mouse, A21235, 1868116; and anti-hamster, A21451, 1572558, Invitrogen), biotinylated secondary antibodies (anti-rat ...
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bioRxiv - Microbiology 2020Quote: ... before being incubated with Alexa Fluor-conjugated secondary antibodies (streptavidin 488 nm, mouse 594 nm, rabbit 647 nm, Life Technologies) for 1 hour in the dark at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections were incubated with secondary antibody Alexa Fluor 488 donkey anti-rabbit (1:250, A-21206, Invitrogen, Landsmeer, the Netherlands) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... all wells were processed for 1 h with appropriate fluorescent secondary antibodies (Alexa Fluor 594 donkey anti-rabbit (1: 1,500, Life Technologies); Alexa Fluor 488 donkey anti-chicken (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with an AlexaFluor-488 conjugated donkey anti-rabbit antibody (Molecular Probes; A-21206; final dilution = 1:1,000). At the end of the immunostaining protocol ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation in a 1:10,000 dilution of HRP-conjugated goat-anti-rabbit secondary antibody (Thermo Fisher #65-6120). Blots were treated with Pierce ECL Western Blotting Substrate (Thermo Fisher #32106 ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with secondary antibodies diluted in blocking buffer for 45 min (1:1000 Alexa-fluor 546 conjugated to mouse or rabbit, Invitrogen) followed by three PBS washes ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2-hour incubation with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen]; 1:200 Alexa 488 anti-chicken [Invitrogen]). Following three additional 10-min washes in PBST ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to wells containing primary antibodies (1:5000 rabbit anti-c-Fos [SySy]; 1:500 chicken anti-GFP [Invitrogen]) and allowed to incubate on a shaker overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The cells were then washed 3x PBS and incubated with species-specific Alexa Fluor 488 conjugated goat anti-rabbit or Alexa Fluor 594 conjugated goat anti-mouse secondary antibodies (1:300; Invitrogen). The cells were then washed 3x PBS and mounted on a 1.5 mm coverslip with a Prolong™ Gold Antifade Mountant with DAPI.
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bioRxiv - Microbiology 2021Quote: ... secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 from Invitrogen and goat anti-mouse Alexa Fluor 647 from Invitrogen) were added and incubated for 30 min protected from light ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were then washed 3 times with PBS and incubated with Alexa Fluor 488-conjugated anti-rabbit secondary antibodies (1:10000; A32731; Invitrogen) for 1 h at room temperature to visualize the antibody reactions ...
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bioRxiv - Cancer Biology 2020Quote: ... Coverslips were then washed by rocking for 3x quick and 3x 10min in PBS-T and incubated with donkey anti-rabbit or anti-mouse secondary antibody (Invitrogen) for 1hr at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... The secondary antibodies used in our study were AlexaFluor 488- or 647-labeled donkey anti-rabbit (1:400; Thermo Fisher), AlexaFluor 488- ...
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bioRxiv - Cancer Biology 2021Quote: ... The precleared lysates were then incubated at 40C overnight with the immunoprecipitating antibody (Supplementary Table S1) or with the IgG isotype control {Rabbit Isotype Control (Thermofisher, Cat ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for immunofluorescence detection in confocal and SIM microscopy were Alexa Fluor Rabbit-anti-Mouse 568 (Invitrogen, A11004) and Donkey-anti-Mouse 647 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: Freshly prepared solubilized proteins were incubated o/n at 4°C with affinity-purified rabbit anti-P2X4 antibodies (Alomone) cross-linked to magnetic beads (Dynabeads, Invitrogen). The flow through was discarded and the beads washed 5 times with wash buffer (CL48 diluted ¼ in PBS and supplemented with complete protease inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then PBS washed and incubated with the appropriate fluorophore-conjugated secondary antibodies in blocking solution (Donkey anti-Rabbit, Alexa Fluor 568, A10042, Invitrogen, Eugene ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were then washed 4× 2 h in PBT and incubated overnight in PBT supplemented with 1:500 goat anti-mouse or goat anti-rabbit secondary antibodies coupled either to Alexa Fluor 488 or 568 (Invitrogen – Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were then stained with secondary antibodies: Alexa Fluor 488 anti-mouse or Alexa Fluor 488 anti-rabbit (ThermoFisher Scientific) for 1.5 hours at room temperature and washed in the same manner ...