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Citations for Thermo Fisher :
5301 - 5350 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... The biotin-dUTP and digoxigenin-dUTP were detected using streptavidin-Alexa 488 (Invitrogen, San Diego, Calif., USA) and anti-digoxigenin-rhodamine (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The biotin-dUTP and digoxigenin-dUTP were detected using streptavidin-Alexa 488 (Invitrogen, San Diego, Calif., USA) and anti-digoxigenin-rhodamine (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Biotin-tagged DNA nicks were visualized using Alexa488-or Alexa647-conjugated streptavidin (Molecular Probes, diluted 1/1000) during the incubation with the secondary antibody.
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bioRxiv - Bioengineering 2022Quote: ... NHS-PEG4-Biotin (catalog no. 21330) and HRP-conjugated streptavidin (catalog no. N100) were purchased from Thermofisher. DAB (catalog no ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface proteins were biotinylated with 0.5mg/ml EZ-link-Sulfo-NHS-SS-Biotin (Thermo Fisher, A39258) in a PBS buffer with 2.5mM CaCl2 and 1mM MgCl2 on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated peptides (Biotin-PNTAYQK(Ac)QQDTLI) previously immobilized on magnetic Streptavidin beads (Dynabeads MyOne Invitrogen™ 65601) were incubated in presence or absence of SIRT1 −/+ NAD+ (0.5 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... click chemistry reactions were performed for 1.5 h in the dark (10 μM Biotin-azide [Thermo Fisher, B10184] ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 RBD (BEI Resources NR-52309) was biotinylated with Sulfo-NHS-LC Biotin (Thermo Fisher), then incubated with 1μm fluorescent neutravidin beads (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Restricted-DNA fragments were filled-in with 15 nmol each of biotin-14-dATP (cat. 19524016, Thermofisher), dTTP ...
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bioRxiv - Developmental Biology 2023Quote: Biotin-labeled cDNA was synthesized and then hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... EZ-Link™ Sulfo-NHS-Biotin (100uL A39256, Thermo Fisher Scientific, 1 mg/0.4 mL in PBS). After 30min circulation ...
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bioRxiv - Microbiology 2024Quote: Primary amines of recombinant EtpA were biotinylated with EZ-Link Sulfo-NHS-LC-LC-Biotin (ThermoFisher 21388) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The bat albumin was biotinylated using EZ-Link® NHS-SS-Biotin (Thermo Fisher Scientific, Ref#21441) following a protocol recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... and cells were replenished with a complete medium containing FBS and 50 uM biotin (Thermo Scientific #29129). Cells were lysed 18 hours after treatment for protein collection and analyzed by immunoblotting ...
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bioRxiv - Molecular Biology 2024Quote: ... Excess biotin reagent was removed using 7K molecular weight cut-off Zeba Spin Desalting Columns (Thermo Scientific). Concentrations given are for Ydj1 dimers and for Ssa1 and luciferase monomers.
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bioRxiv - Molecular Biology 2024Quote: ... Yd1 proteins were labeled using a 1.5-fold excess of NHS-PEG4-Biotin (Thermo Scientific, Life Technologies). Excess biotin reagent was removed using 7K molecular weight cut-off Zeba Spin Desalting Columns (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Yd1 proteins were labeled using a 1.5-fold excess of NHS-PEG4-Biotin (Thermo Scientific, Life Technologies). Excess biotin reagent was removed using 7K molecular weight cut-off Zeba Spin Desalting Columns (Thermo Scientific) ...
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bioRxiv - Genomics 2024Quote: ... Medium was furthermore supplemented with 250 nM Rapalog 1h before harvest and 50 µm Biotin (Invitrogen, B20656) 30 min before harvest ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated in surface biotinylation buffer (0.2 mg/ml sulfo-NHS-SS-biotin in DPBS, Thermofisher#A39258) for 45 minutes at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit (1:500 goat anti-rabbit, ThermoFisher A11012) conjugated with two-photon-excitable fluorophores (488nm and 594nm ...
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bioRxiv - Microbiology 2024Quote: ... or rabbit (Donkey anti-rabbit AF594, Invitrogen, Cat# A11012). Cells were washed in 1x PBS ...
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bioRxiv - Bioengineering 2020Quote: The detection antibody (dAb) was site-specifically labeled with R-Phycoerythrin (R-PE) using a SiteClick R-PE Antibody Labeling Kit (Thermo Fisher Scientific) according to the kit manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies used throughout this work include the anti-pan-MHC-I antibody W6/32 (purified antibody from BioXcell, directly conjugated with AlexaFluor 647 via Molecular Probes kit); anti-CD147 (clone HIM6 ...
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bioRxiv - Cancer Biology 2020Quote: ... 65 million cells were stained with the anti-pan-MHC-I antibody W6/32 (purified antibody from BioXcell, directly conjugated with AlexaFluor 647 via Molecular Probes kit), washed 3x in RPMI/FBS-containing staining buffer ...
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bioRxiv - Immunology 2020Quote: ... Binding of the primary antibody was detected using peroxidase-conjugated secondary antibodies and a SuperSignal West Dura chemiluminescent substrate kit (Thermo Scientific, 34075) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... The resulting supernatant was incubated with magnetic Dynabeads pre-coupled with antibodies using the Dynabeads antibody coupling kit (Thermo Fisher Scientific, 14311D) for 1.5 hours at 4°C on a rotor ...
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bioRxiv - Immunology 2023Quote: ... The purified anti-IFN-λ antibody was conjugated in-house with Alexa Fluor 647 by following the instructions of the antibody labeling kit (Invitrogen, A20186). The obtained labeled anti-IFN-λ antibody solution was used at 1d200 for staining ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoaffinity magnetic beads were prepared by coating antibodies onto Dynabeads® M-270 Epoxy beads using the Dynabeads Antibody Coupling Kit (14311D, ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Half maximal inhibitory concentrations of Cmpd 1 were determined by Z’-Lyte biochemical assays (Life Technologies SelectScreen™ Kinase Profiling Services, Thermo Fisher Scientific). Kinome selectivity was determined at 1 μM Cmpd 1 concentration against a panel of 119 kinases.
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bioRxiv - Genetics 2021Quote: ... and axial (z) directions were estimated form bead image stacks using 500 nm sized four color-stained beads (Tetraspecks, Thermo Fisher, Catalog number T14792). The center plane is defined as the plane with the largest diameter signal within a bead and was determined manually in an unprocessed bead image stack ...
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bioRxiv - Microbiology 2022Quote: ... Negative and positive mode data were collected at a mass range of 70–1050 m/z in centroid data mode on a Thermo QExactive (Thermo Fisher Scientific, Waltham, MA). Fragmentation spectra (MS/MS ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Fezf2 and rabbit anti-involucrin were revealed with Cy3-conjugated anti-rabbit IgG (Invitrogen) and biotinylated anti-TPA was revealed with Alexa Fluor 488-conjugated streptavidin (Invitrogen) ...
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bioRxiv - Plant Biology 2020Quote: ... the fixed sections were probed with the anti-PPDK primary rabbit polyclonal antibody (provided by Dr. Chris Chastain, Minnesota State University-Moorhead) and the Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen) secondary antibody ...
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bioRxiv - Plant Biology 2020Quote: ... in the blocking solution and added to the seedlings for 1h incubation at37°C.Then seedlings were washed twice with MTSB buffer and incubated with the secondary antibody goat anti-Rabbit IgG DyLight 633 (Invitrogen) in MTSB buffer for 1h at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... The secondary antibody (AlexaFluor 488- or AlexaFluor 546-conjugated goat anti-mouse or anti-rabbit IgG; Life Technologies, Carlsbad, USA) was diluted with blocking/dilution buffer to a concentration of 5 µg mL−1 ...
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bioRxiv - Biophysics 2021Quote: ... HRP-conjugate goat-anti-rabbit secondary antibody was used (1:15,000 dilution, Pierce) and developed with SuperSignal West Pico Plus chemiluminescent substrate (Thermo Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... Then, samples were rinsed and incubated with an Alexa Fluor 488 conjugated secondary antibody (Donkey, Rabbit IgG #A21206 (Life Technologies), 1/200) ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated with the following secondary antibodies for 1 h: Alexa 546-conjugated goat anti-rabbit IgG (1:1,000, Thermo Fisher Scientific Cat# A-11010 ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were used at 1 μg/ml for goat anti-rabbit and sheep anti-mouse linked horseradish peroxidase (Invitrogen). Blots were developed with SuperSignal West Pico Chemiluminescent Substrate (Pierce).
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used were goat anti-rabbit and goat anti-mouse conjugated to Alexa Fluor 488 or 568 (1:1000, Invitrogen). To distinguish individual cells for quantification of MUC5AC-positive cells ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used were 1:400 anti-rabbit Alexa 488 and 1:250 anti-mouse Alexa 594 from Invitrogen. The cells were washed thrice in 1X PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody with Alexa Fluor 594 (A27016) 1:500 and DAPI (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... The membranes were then incubated with IRDye 800 CW goat-anti rabbit secondary antibodies (1:5000, #31460, Thermo Fisher Scientific) for 90 min at room temperature and visualized with an Odyssey scanner (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... and alexa 568-conjugated goat anti-rabbit IgG (H+L) antibody (no. A-11036, Thermo Fisher Scientific, 1:1,000 dilution).
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bioRxiv - Cell Biology 2019Quote: ... or a 615/20 nm bandpass emission filter (Semrock) for the Alexa Fluor 568 goat anti-rabbit secondary antibody (Invitrogen). The 615/20 emission filter was used to collect the fluorescent signal from the Rhodamine-Phalloidin while the 520/35 nm emission filter was used to detect the krt4:EGFP and lepb:EGFP fluorescence ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 488 or 568 donkey anti-mouse or donkey anti-rabbit secondary antibodies (A10037, A21202, A10042 and A21206) were purchased from ThermoFisher Scientific (Rockford,Il).
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bioRxiv - Cell Biology 2020Quote: ... the intestines were incubated 2 h at room temperature with secondary antibody Alexa Fluor 488 donkey anti-rabbit IgG (#A21206, Invitrogen) 1:600 ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with anti-rabbit and anti-mouse secondary antibodies that were conjugated to Alexa Fluor 594/568/488 (ThermoFisher Scientific# A21207 ...
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bioRxiv - Cell Biology 2020Quote: ... Respective AlexaFluor 488- and AlexaFluor 594-tagged secondary donkey antibodies to rabbit (A-11008 and A-11012) or mouse (A-21202 and A-21203) IgG were from Invitrogen Molecular Probes (Eugene ...
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bioRxiv - Cell Biology 2020Quote: ... the secondary antibody was added in the same way as the primary antibody (anti-rabbit-Alexa fluor 488, Alexa fluor 568 or Alexa fluor 633, Invitrogen) for 45 min at room temperature ...