Labshake search
Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Olfactomedin 4 OLFM4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... primary antibodies were incubated for three nights at 4°C followed by incubation with donkey anti-mouse or anti-rabbit IgG secondary antibodies (1:1000, Life Technologies). Fluorescent images were acquired with an Olympus FV1000 at 2048 x 2048 pixel resolution using a 40x objective lens ...
-
bioRxiv - Neuroscience 2020Quote: ... cells were incubated for 1 h with Alexa Fluor 488-conjugated secondary antibody and nuclei were counterstained for 15 min with 4 µg/ml Hoechst 33342 (Molecular Probes). 96 and 384 well plates were scanned with Cellvoyager CV6000 (Yokogawa Inc.) ...
-
bioRxiv - Cell Biology 2022Quote: ... the samples were rinsed three times in the blocking solution and incubated for 4 hours in goat polyclonal secondary antibody to mouse IgG conjugated with AlexaFluor 555 (1:100, Invitrogen A31570) and donkey polyclonal secondary antibody to rabbit IgG conjugated with AlexaFluor 647 (1:100 ...
-
bioRxiv - Cell Biology 2022Quote: ... for 1 hr at 4°C, then proceeded for immunoprecipitation with specific antibodies (Rab5A, Rab 11 and Rab7) and Protein A/G bead (Thermo Fisher) for overnight at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... Cell Signaling)] at 1:1,000 in blocking buffer overnight at 4°C with rocking and subsequently with HRP-conjugated goat α-rabbit or α-mouse secondary antibodies at 1:5,000 (ThermoFisher) in blocking buffer for 2 h at room temperature with rocking ...
-
bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...
-
bioRxiv - Developmental Biology 2019Quote: ... and sections were incubated overnight at 4°C with the following primary antibodies: rabbit anti-GFP (Invitrogen A-11122, 1:1000), mouse anti-Satb2 (Abcam ab51502 ...
-
bioRxiv - Zoology 2019Quote: ... preincubated for 30 min at room temperature in PTX+NGS and incubated overnight at 4°C in secondary antibodies (goat anti-mouse conjugated with AlexaFluor647 and goat anti-rabbit conjugated with AlexaFluor488, Life Technologies) dissolved in Ptx+NGS in 1:250 concentrations ...
-
bioRxiv - Neuroscience 2019Quote: ... tissues were incubated overnight at 4°C in Alexa Fluor 488-conjugated goat anti-rabbit Ab (1:200) secondary antibody (Life Technologies) in PBS containing 10% normal sheep serum ...
-
bioRxiv - Developmental Biology 2019Quote: ... Larvae were rinsed in PBST three times for 20 min and incubated overnight at 4°C with secondary antibodies (anti-mouse 1:500 IgG-Alexa Fluor 488, 1:500 IgG Alexa Fluor 594, Molecular Probes; anti-rabbit IgG-Alexa Fluor 488 ...
-
bioRxiv - Developmental Biology 2020Quote: ... overnight at 4°C and further stained with 1:200 Alexa Fluor 633 Goat anti-Rabbit IgG (H+L) Secondary Antibody (Invitrogen A21071) at a 20°C for 3 hours ...
-
bioRxiv - Neuroscience 2019Quote: ... for 80 minutes (4 × 20 min) at room temperature before secondary antibodies (donkey anti-rabbit Alexa Fluor 1:1000, Life Technologies Cat No ...
-
bioRxiv - Developmental Biology 2020Quote: ... while the remainder was immunoprecipitated for 2 hr at 4°C in the following antibodies: rabbit anti-GFP (Invitrogen, A-11122), rabbit anti-Cen ...
-
bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C with an anti-CD63 primary antibody (1:20 dilution, Thermo Fisher Scientific, cat. # MA1-19281) in blocking buffer ...
-
bioRxiv - Systems Biology 2020Quote: ... cells were stained at 4°C o/n in 50 µL of the 1st antibody diluent (rabbit anti-ZO-1, 61-7300, Thermo Fisher Scientific Inc. ...
-
bioRxiv - Genomics 2020Quote: ... samples and the rest was used for immunoprecipitation overnight at 4°C with rotation with 16 μg of anti-GFP antibody (Invitrogen A11122). The chromatin was then cleared by centrifugation (12,000 g for 10 minutes at 4°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... Chromatin with antibody were rotated overnight at 4 °C before 1:40 protein A/G agarose beads (Thermo Fisher Scientific, #20423) were added and incubated for another 6 hrs ...
-
bioRxiv - Cell Biology 2020Quote: ... 1:200) respectively at 4°C overnight and secondary antibody conjugated to Alexa Fluor 594 or Alexa Fluor 488 (Life Technologies) at room temperature for 1h ...
-
bioRxiv - Physiology 2019Quote: ... was incubated overnight at 4°C and followed by Alexa Fluor 488-labeled anti-rabbit antibody (Thermo Fisher Scientific, A-21235). Next ...
-
bioRxiv - Developmental Biology 2020Quote: ... Sheared chromatin was cleared and incubated overnight at 4 degrees with primary antibodies that were pre-incubated with protein G magnetic DynaBeads (Thermo Fisher). Primary antibodies used included anti-H3K27Ac (9ug/sample ...
-
bioRxiv - Physiology 2020Quote: ... Sections were incubated overnight (4 °C) with the respective primary antibodies: Anti-Ly6G rat monoclonal (1:100; Affymetrix, Santa Clara, CA) for neutrophils ...
-
bioRxiv - Cancer Biology 2019Quote: ... or IgG (mouse or rabbit based on the antibody used for the immunoprecipitation) for 4 hours followed by addition of 20 μl protein A/G agarose beads (ThermoFisher Scientific) for 2 hours ...
-
bioRxiv - Plant Biology 2019Quote: ... The slides were incubated overnight at 4°C with a primary antibody specific to GFP (1/200, Life Technologies, A-11122) then for 2 hours with goat-anti-rabbit-AlexaFluor488 secondary antibody (Life Technologies ...
-
bioRxiv - Neuroscience 2019Quote: ... Sections were incubated for 4 h at room temperature in fluorescent conjugated secondary antibodies (Alexa Fluor 488 goat anti-mouse (Th; 1:500; Invitrogen; A10667) and Alexa Fluor 594 goat anti-rabbit (DsRed ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were washed in PBS-0.5%Triton X-100 at RT before overnight incubation at 4°C with Alexa555-conjugated donkey-anti-rabbit antibody (Invitrogen, #A32794) in PBS containing 5% NHS ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 days at 4°C with rabbit anti-GFP antibody (1:2,000; Thermo Fisher Scientific, USA). The sections were then incubated with biotinylated donkey anti-rabbit IgG antibody (1:1,000 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were washed with 1x PBS and secondary antibodies diluted in blocking solution were added 4 hrs at RT: anti-Rabbit 555 (1:800; Invitrogen; A31572), anti-Chicken Cy2 (1:800 ...
-
bioRxiv - Biochemistry 2021Quote: ... resuspended in 100 μL of secondary antibody (1:500 α-mouse IgG-Alexa647 [ThermoFisher, Waltham, MA] in TBST with 4% BSA), and incubated in the dark for 60 minutes at room temperature ...
-
bioRxiv - Biochemistry 2020Quote: ... or mouse monoclonal anti-N-Cadherin clone GC-4 followed by AlexaFluor 647-conjugated goat anti-mouse antibody (1:200 dilution, ThermoFisher Scientific). Staining was performed for 30 min at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... embryos were washed five times in TBSN/BSA for one hr at 4°C whilst rotating and incubated with secondary antibodies overnight at 4°C at a dilution of 1:400 (Alexa Fluor 488 goat anti-mouse (Invitrogen, #A11001) and 1:400 Poly-HRP-conjugated secondary antibody from Tyramide SuperBoost Kits with Alexa Fluor 568 (ThermoScientific ...
-
bioRxiv - Immunology 2021Quote: ... targeting four different T cell populations as well as 4 μg mL−1 of goat-anti-mouse IgG AF488 secondary antibody (Thermo Scientific). Acquisition and data analysis were performed as mentioned above.
-
bioRxiv - Immunology 2020Quote: ... HNSCC PBL or healthy donor PBL were stained with fluorescently labeled antibodies for 25 mins at 4°C in PBS (Thermo Fisher) supplemented with 2% FBS (Atlanta Biologicals ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.3% Triton X-100 in PBS) and incubated overnight (4 °C) with primary antibodies against TH (OPA1-04050 1:1000, Thermo Scientific) and HA (3F10 1:200 ...
-
bioRxiv - Immunology 2022Quote: ... GMECs and fibroblasts were incubated overnight at 4°C with cytokeratin-18 monoclonal antibody (# Cat. No. MA1-19031, Thermo Scientific, USA). MCF 10A cells were incubated with CKT-18 Monoclonal Antibody (# Cat ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were immunoprecipitated with the antibody-bead complex on a rotating shaker overnight at 4°C with the addition of 100 U/mL Ribolock (ThermoFisher Scientific). RNA was extracted from beads by using TRIzol (ThermoFisher Scientific ...
-
bioRxiv - Biophysics 2022Quote: ... the antibody produced in-house from HPC-4 hybridoma, ATCC HB-9892, and labeled with Alexa Fluor 647 NHS Ester, Thermo Fisher), showing superior Wnt display on the hFzd5CRD-1TM cells ...
-
bioRxiv - Cancer Biology 2022Quote: ... sonicated chromatin was incubated overnight at 4°C with antibodies that had first been coupled to Protein G beads (Thermo Fisher). After incubation ...
-
bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
-
bioRxiv - Neuroscience 2022Quote: ... Slices were then dipped in the secondary antibody-containing buffer with the nuclear dye 4′,6-diamidino-2-phenylindole (DAPI, Cat# D1306, Life Technologies-Invitrogen ...
-
bioRxiv - Neuroscience 2022Quote: ... blocked again for 2h (10% CAS-Block / 90% PBT) and incubated overnight at 4°C with secondary antibody (1:250, Alexa-Fluor-594, ThermoFisher A32742) diluted in 100% CAS-Block ...
-
bioRxiv - Neuroscience 2022Quote: ... Supernatants were then immunoprecipitated on a rotator for two hours at 4 °C with antibody pre-bound to 17 μl Protein A Dynabeads (Invitrogen, 10001D). The following antibodies was used ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 mg of total protein was incubated with the DNA-PKcs monoclonal antibody (25–4) and Protein A/G beads (Thermo Fisher) overnight at 4° C with mixing ...
-
bioRxiv - Neuroscience 2022Quote: ... Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568, 1:200, Invitrogen, A11041; AlexaFluor 647, 1:200, Invitrogen, A21236). Whole muscles were dehydrated in a series of 25% ...
-
bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used were as follows: AlexaFluor-647-conjugated donkey anti-rabbit IgG (4 µg/ml, Invitrogen, catalog # A-31573, RRID:AB_2536183), AlexaFluor-488-conjugated goat anti-guinea pig IgG (4 µg/ml ...
-
bioRxiv - Neuroscience 2022Quote: ... Tissues were then incubated for 4 days at 37°C with a secondary antibody (donkey anti-rabbit AF488, Invitrogen, 1:1,000) in 1X PBS with 0.2% Tween 20 ...
-
bioRxiv - Physiology 2022Quote: ... Slides were washed with 0.01% PBS-T and incubated with secondary antibodies (Supplemental Table 4) with Hoechst (Thermo Fisher Scientific H1399) for 1 hour at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... at 1:250 dilution overnight at 4°C and anti-mouse AlexaFluor-555 secondary antibody (1:1000, ThermoFisher Scientific, #A-21424) for 1 hour at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were washed three times with PBS or PBST for 10 min and incubated with secondary antibody anti-mouse (4 μg/ml, Thermofisher #A11030) or anti-rabbit (4 μg/ml ...
-
bioRxiv - Molecular Biology 2023Quote: ... then washed 4× 5min in PBS with subsequent incubation with secondary antibodies (Alexa-Fluor 488, 555, 568 or 647 (Molecular Probes or Invitrogen)) ...
-
bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 hours at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...