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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Olfactomedin 4 OLFM4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Immunoblotting of MSD2-mCherry was performed after native-PAGE or SDS-PAGE (12.5% separating gel and 4% stacking gel) with anti-mCherry primary antibody (Invitrogen, cat. n. M11217) diluted to 1:5,000 in Tris-buffered saline with 0.1% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... blocked for 20 min at room temperature and stained overnight at 4 °C using an anti-V5 antibody (1/500; Invitrogen, Cat R96025) or anti-FLAG antibody (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... Incubations were performed for 14h at 4° C followed by incubation with secondary antibodies: Alexa 568 anti-mouse 1:200 (Thermo Fisher A10037), and Alexa 488 anti-goat 1:200 (Thermo Fisher A11055 ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated 20 min at 4°C with AF647-conjugated goat anti-human IgG antibodies (1:1000 dilution; Thermo Fisher Scientific) and LIVE/DEAD Fixable Viability dye Aqua (1:1000 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were blocked with 4% goat serum in PBS for 1 hr and then incubated with primary antibodies against cTnT (Thermo Fisher Scientific), α-actinin (Abcam) ...
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bioRxiv - Biophysics 2020Quote: ... 60 or 90 min post treatment using 4% paraformaldehyde and next stained with primary antibodies for STIM1 (#PA1-46217, Thermo Fisher Scientifc), Orai1 (#NBP1-75523 ...
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bioRxiv - Cell Biology 2021Quote: ... at 4°C overnight and then incubated with horseradish peroxidase (HRP)-conjugated secondary anti-mouse or rabbit antibodies (Thermo Fisher, Waltham, MA). Protein expression was detected by using enhanced chemiluminescence (ECL ...
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bioRxiv - Microbiology 2020Quote: ... Pre-cleared lysates were further incubated at 4°C overnight with the indicated antibodies (1 to 3 μl) and protein A agarose or protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitates were washed three times with RIPA buffer (LSB ...
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bioRxiv - Genomics 2021Quote: ... Supernatants were then immunoprecipitated on a rotator for two hours at 4 °C with antibody pre-bound to 17 μl Protein A Dynabeads (Life Technologies 10001D). The following antibodies were used ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Physiology 2021Quote: ... and then incubated overnight at 4°C with AF594-conjugated goat anti-mouse IgG1 secondary antibody (A21125, Invitrogen, USA; 1:500 dilution) and AF488-conjugated α-bungarotoxin (B13422 ...
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bioRxiv - Physiology 2021Quote: ... and then incubated overnight at 4°C with AF594-conjugated goat anti-mouse IgG1 secondary antibody (A21125, ThermoFisher, USA; 1:500 dilution), Cy5 goat anti-rabbit IgG (H&L ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for overnight with primary antibody at 4 degrees and further incubated with secondary antibodies (1:500) for 1 hour followed by 4′,6-diamidino-2-phenylindole (DAPI) or phalloidin 488 (1:400, Thermo Fisher, A12379) staining ...
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bioRxiv - Neuroscience 2021Quote: ... a series of every 6th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with rabbit anti-GFP monoclonal antibody (1:500, G10362, Thermo Fisher Scientific) and rabbit anti-mKO polyclonal antibody (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing 1 mg of protein were incubated with anti-HA antibody (1:100) for 1 h at 4 °C followed by incubation with Protein G Dynabeads (Thermo Fisher Scientific) for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were counterstained with 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine (DAPI) and appropriate secondary antibodies conjugated to fluorochromes (Thermo Fisher Scientific) were applied for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were then washed 4 × 5 min with PBS followed by overnight incubation at 4°C with the following secondary antibody dilutions in blocking solution: Alexa 488-goat anti-rabbit (1:1000; Thermo Fisher Scientific) and Alexa 594-donkey anti-mouse (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 4 μg of anti-HECTD1 antibody or normal Rabbit IgG was coupled to Dynabeads® magnetic beads protein G (ThermoFisher Scientific, #10003D) for 1 hr at RT ...
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bioRxiv - Biochemistry 2020Quote: ... The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific) for an additional 2 hours with constant rotation ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the brains were rinsed in PBS for 4 × 15 min before being incubated in the Cy2-conjugated anti-mouse secondary antibody (dilution 1:300 in PBSX; Invitrogen, Eugene, OR), at 4 C for 1 d ...
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bioRxiv - Microbiology 2021Quote: ... Samples were fixed with 4% buffered paraformaldehyde at 24 hpi and stained with Monoclonal anti-Flag M2 antibody (1:500, Sigma, Thermo Scientific™) and alexa 488 anti-mouse secondary antibody to visualize expressing Chlamydia ...
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bioRxiv - Genomics 2021Quote: ... Cat# ABIN6952432) overnight at 4 ?C followed by staining with Alexa Fluor 647-conjugated secondary antibody (goat anti-mouse, Thermo Fisher, A21235) and DAPI (Thermo FIsher) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed three times with PBS and incubated for 2 h at 4°C with appropriate combinations of AlexaFluor-conjugated secondary antibodies (Invitrogen,1:2000) for 2 hours at 4C protected from light ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody incubation was also performed overnight at 4°C with donkey anti-mouse Alexa 647 (1:250, Invitrogen, Cat. # A-31571).
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C in a primary antibody/0.5% BSA/PBS-T solution of rabbit anti-TH (1:1000, Invitrogen, Carlsbad, CA, 701949). Sections were then washed 6×10 minutes in PBS-T before incubating in secondary antibody/0.5% BSA/PBS-T solution of Alexa568 donkey anti-rabbit (1:2000 ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane was washed 5 times 5 min in TBS-T prior to overnight incubation at 4°C with 1° anti-Flag antibody (1:500, ThermoFisher, PA1-984B). The next morning ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labeled with DAPI together with the secondary antibody (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10.000, Thermo Fisher Scientific, #D1306). The following secondary antibodies were used ...
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bioRxiv - Immunology 2022Quote: ... mBECs treated with IFNγ were fixed with 4% paraformaldehyde in PBS for 15 minutes and stained with antibodies against Zona occludens-1 (ZO-1, 1:250 Invitrogen 33-9100) and CXCL10 (1:500 Abcam ab9938) ...
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bioRxiv - Immunology 2022Quote: ... The leaflets were then incubated for 2 days in the dark at 4°C with goat anti-Rat IgG Cross-adsorbed Alexafluor 594 secondary antibody dilution 1:500 (#A11007, Thermo Fisher Scientific), washed 3 times in 1 mL PBS (pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were processed for 48 hours at 4 degrees with the primary antibody in blocking solution (1:1250, rabbit anti-Esr1, Invitrogen PA1-309), rinsed 3×10 minutes in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4°C in a 1:500 dilution of primary antibody: Tyrosine Hydroxylase (TH, mouse, Invitrogen; MA1-24654) and the immediate early gene protein c-Fos (c-Fos ...
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bioRxiv - Neuroscience 2022Quote: ... The fixed cultures were washed twice in 0.01 M PBS and incubated overnight at 4 °C with donkey anti-rabbit Alexa488-labeled secondary antibody (1:1000; Invitrogen, Cat# A21206) in 0.01 M PBS with 10% NGS ...
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bioRxiv - Microbiology 2022Quote: ... and intracellular markers were stained with monoclonal antibodies for 45 minutes at 4°C with FoxP3 (FJK-16S) and IL-10 (JES5-16E3) (All antibodies were from Thermo Fisher Scientific). Cells were resuspended in PBS with 2% NCS and 2 mM EDTA ...
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bioRxiv - Biochemistry 2022Quote: ... which were supplemented with additional 800 μL of RIPA buffer and incubated overnight at 4°C with 15 μL of antibody-coupled protein G Dynabeads (Thermo Fisher Scientific). Before adding the cleared chromatin ...
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bioRxiv - Biophysics 2022Quote: ... rinsed 3 times in PBT and incubated for 4 hours at room temperature with anti-mouse secondary antibody coupled to Dylight 488 (Thermo Fisher Scientific) at 1:1,000 in PBT for 4-5 hours ...
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bioRxiv - Biochemistry 2024Quote: ... HBMEC were incubated overnight at 4°C with the primary antibodies (anti-β-catenin, # 71-2700; anti-ZO-1, # 61-7300; anti-CAT1, # PA5-82800, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated over-night at +4°C with donkey anti-sheep secondary antibody conjugated with Alexa Fluor 568 (1:1000, A-11011, Invitrogen, ThermoFisher Scientific) and Streptavidin conjugated with Alexa Fluor 488 (1:2000 ...
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bioRxiv - Cancer Biology 2024Quote: ... then washed again and incubated at 4°C for 30 minutes with primary antibody diluted in 1X Click-iT saponin-based permeabilization and wash reagent (Thermo Fisher, #C10419). Cells were then washed once with PBS with 1% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked with 4% horse serum in PBS for 1 hour and then incubated with primary antibodies against cTnT (Thermo Fisher Scientific), α-actinin (Abcam) ...
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bioRxiv - Microbiology 2024Quote: ... for HSV-1 in blocking buffer for 1 h at 4°C and stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer at RT for 1h ...
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bioRxiv - Molecular Biology 2024Quote: ... antibodies were used with 1% BSA and 0.1% Triton X-100 in D-PBS for overnight incubation at 4°C followed by a secondary incubation of Alexa Fluor 568 antibody (Invitrogen, Cat#A11036) or Alexa Fluor 488 antibody (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Fixed monola were blocked with 4% BSA-PBST and probed with primary antibodies for pSTAT1-Tyr701 (ThermoFisher Scientific MA515071, 1:250 dilution) and Chlamydia heat-shock protein 60 (cHsp60 ...
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bioRxiv - Neuroscience 2024Quote: ... 1h at RT and subsequently incubated overnight at 4°C in primary antibody solution using Rabbit anti-Green-Fluorescent-Protein (GFP; 1:1000 ThermoFisher Scientific, A6455). The following day sections were rinsed three times in PBTriton 0.5% and incubated overnight at 4°C in secondary antibody solution using Goat anti-Rabbit-Oregon Green 488 (1:500 Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... the sections were blocked with 0.1% Triton X-100 and 10% FBS in 1xPBS overnight at 4°C and stained with the following reagents and antibodies: NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI) (Invitrogen), Alexa Fluor™ 647 Phalloidin 1:500 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 1x TBS-T with 3% BSA (Fisher Scientific BP1600): α-SARM1 (BioLegend 696602 ...
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bioRxiv - Cell Biology 2024Quote: ... was done for 12 h at 4 °C and secondary incubation with a fab2 antibody (Alexa Fluor 647-conjugated goat anti-rabbit; Thermo-Fisher Scientific Inc.) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Primary Ab was washed off in PBS triton-X and tissue was then incubated with secondary antibodies at RT for 2-4 hours (Alexa Fluor, Thermo Fisher Scientific). The tissue was then washed again and cover-slipped ...
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bioRxiv - Microbiology 2023Quote: ... for 16 hours at 4°C and followed by secondary Alexa-Fluor 488- or Alexa Fluor-594-conjugated antibodies for another 60 min (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed 4-5 times in PBS for 2 hours then incubated for 3-4 hours with secondary antibody (anti-rabbit Alexa Fluor 568; 1:1000; Invitrogen; Cat #A10042) and NeuroTrace Green Fluorescent Nissl Stain (1:2000 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and then incubated for 4 h at room temperature with secondary antibody (Alexa-Fluor 488 goat anti-rabbit IgG, ThermoFisher #A-11008) diluted 1:500 in blocking solution ...