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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for N 5 Trimethoxysilyl 2 Aza 1 Oxopentyl Caprolactam since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... SK-N-BE (2) and NGP cells were grown in RPMI1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... and N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher), i.e. ...
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bioRxiv - Neuroscience 2023Quote: ... 1X N-2 (Thermo Fisher Scientific, 17502-048], 1X B-27 [Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 0.173% 2-methoxy(polyethyleneoxy)propyltrimethoxysilane (Gelest, SIM6492.7) and 0.62% n- butylamine (Acros Organics) with flowing nitrogen gas for 90 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR on the mouse Zdhhc17 gene using primers spanning exons 1 and 2 (5’-ACCCGGAGGAAATCAAACCACAGA-3’ and 5’-TACATCGTAACCCGCTTCCACCAA-3’) and Sso/Advanced Universal SYBR green supermix (Fisher Scientific) was performed on CFX96 Real Time System (C1000 Touch Thermal Cycler ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... EDTA was added to the samples at a final concentration of 5 mM and the suspension was diluted 1:5 in collection buffer (2 % heat-inactivated fetal bovine serum, Life Technologies; 26140079 ...
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bioRxiv - Neuroscience 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU; 5 mg per kg body weight, Invitrogen) dissolved in sterile phosphate buffer solution (PBS ...
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bioRxiv - Microbiology 2023Quote: ... 5-[3-aminoallyl]-2’-deoxyuridine-5’-triphosphate (aminoallyl-dUTP, Thermo Scientific™) was incorporated into a PCR by the use of a DreamTaqTM DNA Polymerase (Thermo Scientific™ ...
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bioRxiv - Neuroscience 2020Quote: ... n = 5 male HR mice was homogenized in TRIzol Reagent (#15596018, Thermo Fisher Scientific) and Zirconium beads (#Zr0B05-RNA ...
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bioRxiv - Immunology 2020Quote: ... and 5 mg/mL Sulfo-NHS (N-hydroxysulfosuccinimide; Thermo Fisher Scientific; Waltham, MA, USA). Activated microspheres were washed twice in 0.05 M MES (2-(N-morpholino ...
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Microbiology 2022Quote: Copies of the SARS-CoV-2 N gene (genomic) were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2023Quote: ... prior to a one-hour incubation with SARS-CoV-2 N protein antibody (clone 1C7C7) at a 1:300 dilution and a AF594-conjugated Goat-anti-mouse secondary (ThermoFisher, A11005). Slides were counterstained with DAPI and scanned on an Akoya Polaris Vectra imaging system ...
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bioRxiv - Neuroscience 2024Quote: ... All cultures received N2 medium from d0 to 11 (50% MACS Neuromedium, 50% DMEM/F12 containing 1% N-2 supplement [Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... claudin-5 (1 μg/ml) and occludin (2 μg/ml; all from Thermo Fisher Scientific). After incubation with primary antibodies membrane was washed with PBS-T three times on a platform rocker and incubated with horseradish peroxidase-conjugated secondary antibody diluted in PBS-T 1:2000 (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Recovered nuclei were stained with FITC-conujugated α-5-bromo-2’-deoxyurine (Invitrogen, MoBu-1) in staining buffer (2 mM HEPES pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: Tadpoles were immersed in a solution containing 1 mM EdU (5-ethynyl-2’-deoxyuridine; Invitrogen) before fixation ...
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bioRxiv - Cancer Biology 2022Quote: ... collected as single cell suspensions using cell strainer and resuspended in sphere formation medium which was composed of 1:1 DMEM and F-12 nutrient mix supplemented with B-27 and N-2 (Thermo Fisher Scientific, Waltham, MA, USA). 1,000 cells were plated in each well of ultra-low attachment 6-well plates (Corning ...
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bioRxiv - Bioengineering 2021Quote: ... N,N,N’,N’- tetramethyl ethylenediamine and glycylglycine were purchased from Acros Organics (New Jersey, USA). Protogel 30% acrylamide ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 million SK-N-BE(2)-FLUC cells were stained immediately before injection with CellTrace™ Far Red (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... Samples were stained in DAPI (solution from the RNAscope kit) for 5 minutes or O/N in 1:1000 Spy505 (Spirochrome) and then mounted in ProLong™ Gold (Invitrogen, P36930) in between two coverslips (thickness 1.5mm ...
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bioRxiv - Bioengineering 2021Quote: ... N,N′-Diisopropylcarbodiimide (DIC, Fisher Scientific) and 10 equivalents of DIPEA in DMF for 4 h ...
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bioRxiv - Bioengineering 2024Quote: ... N,N-dimethylethanolamine (Fisher Scientific Company), 2-methoxyethanol (Thermo Scientific Chemicals) ...
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bioRxiv - Microbiology 2023Quote: ... The following custom TaqMan gene expression assay IDs were used to assess the expression of RSV N and SARS-CoV-2 N genes: ARU66XH and APNKYWD (Applied Biosystems, CA, USA). A no template control was included in each run ...
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bioRxiv - Cell Biology 2020Quote: ... organoids (after 1 week of culture) were incubated (37°C, 5% CO2, 1 h) with 10 µM EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher Scientific), a nucleoside analog of thymidine incorporated into DNA during active DNA synthesis ...
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bioRxiv - Biophysics 2020Quote: N-(1-pyrene)iodoacetamide (pyrene) was from Life Technologies Corporation (Grand Island ...
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bioRxiv - Biochemistry 2021Quote: ... N-(1-pyrene)iodoacetamide (Thermo Fisher Scientific, Waltham, MA) was added to a final concentration of 40 µM ...
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bioRxiv - Neuroscience 2020Quote: ... Neurotrace 640/660 (1:200) (N-21483, Molecular Probes) or DAPI (1:1000) ...
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bioRxiv - Biochemistry 2021Quote: N-(1-pyrene)iodoacetamide (pyrene) was from Life Technologies Corporation (Grand Island ...
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bioRxiv - Physiology 2022Quote: ... and N-Cadherin (Invitrogen 33-3900, 1:400, mouse) antibodies as described in19 ...
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bioRxiv - Microbiology 2024Quote: ... 1% n-Dodecyl-β-D-Maltoside (DDM, ThermoFisher ScientificTM), containing half tablet of EDTA-free protease inhibitors (Roche cOmplete) ...
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bioRxiv - Developmental Biology 2024Quote: ... and anti-N-cadherin (1:100, Invitrogen, Life Technologies). After several washes in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... and anti-N-cadherin (1:100, Invitrogen, Life Technologies). After several washes in PBS ...
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bioRxiv - Genomics 2021Quote: ... consisting on regular DMEM supplemented with 1x N-2 supplement (Thermo Fisher Scientific #17502048), 1x B27 supplement (Thermo Fisher Scientific #12587010 ...
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bioRxiv - Bioengineering 2022Quote: ... [2-(N-morpholino)ethanesulfonic acid] (MES) buffered saline pack was purchased from Thermo Scientific. Chemicals sensitive to moisture ...
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bioRxiv - Neuroscience 2024Quote: ... the media was changed to neural medium with N-2 supplement (Life Technologies, 17502048) supplemented with BDNF (20 ng ml−1 ...
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bioRxiv - Immunology 2024Quote: ... washed with CSB and left O/N in 2% formaldehyde (PFA) (Thermo Fisher Scientific) at 4°C.
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bioRxiv - Bioengineering 2022Quote: ... Slides were dehydrated by sequential submersion in 100% ethanol (2× 5 min) and SafeClear II (2× 5 min, Fisher Scientific) before being mounted in Cytoseal 60 (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... and 52h after plating 5 µM 5-ethynyl-2′-deoxyuridine (EdU, Life Technologies) was added for 20 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... #2: 5’-gatcccGATTACTCGGCCATGATCAAAgcttcctgtcacTTTGATCATGGCCGAGTAATCttt ttta-3’ and 5’-agcttaaaaaaGATTACTCGGCCATGATCAAAgtgacaggaagcTTTGATCATGGCCGAGTAATgg-3’ were purchased from Invitrogen. The DNA oligo pairs were annealed and inserted into pEntryCla12-chickU6 shuttle vector using BamHI/HindIII site.
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bioRxiv - Cell Biology 2020Quote: ... labelled with the fluorescent lipid dye BODIPY® FL Maleimide [BODIPY® FL N-(2-Aminoethyl) Maleimide] (Thermo Fisher Scientific; 1:200) and incubated at room temperature in the dark 10 minutes prior to analysis ...
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bioRxiv - Immunology 2024Quote: ... Four microliters RNA was used RT- qPCR to detect and quantify N gene of SARS-CoV-2 using the TaqMan RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) with the following primers and probes for the N-gene ...
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bioRxiv - Physiology 2020Quote: ... and incubated with 100 mM 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (6-NBDG) (Life Technologies) in 10 nM Tris/HEPES buffer containing 150 mM KCl or 150 mM NaCl for 30 minutes at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... Final water-PAAm mixtures were crosslinked with 0.5% APS and 0.15% N,N,N′,N′-tetramethylethylenediamine (ThermoFisher, 17919). 50 μL of the mixtures were added to each glass and allowed to crosslink for 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... resuspended in dichloromethane and derivatized using N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) containing 1% trimethylchlorosilane (TMCS; Thermo Scientific). GC-MS was performed using an Agilent (Santa Clara ...
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bioRxiv - Neuroscience 2020Quote: ... The water contained 1 mg/mL 5-ethynyl-2-deoxyuridine (EdU) (Thermo Fisher Scientific, Cat. # E10187) and 1% sucrose ...
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bioRxiv - Physiology 2021Quote: ... 1-2 µg of vector DNA was mixed with 5 µl of Lipofectamine™ (Thermo Fisher) in OPTIMEM-I media (GIBCO ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were loaded with Fura-2 AM at 1 μg/mL (108964-32-5, Life Technologies) in HBSS or Fluo-4 AM at 10 μM in (ThermoFisher ...