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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 0.1mM beta-mercaptoethanol (Gibco, 31350-010), and 1U/μl leukaemia inhibiting factors (Cell guidance system ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.1% beta-mercaptoethanol (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... followed by Beta Actin (Invitrogen MA515739). Bands were visualized using chemiluniscence (Supersignal West Pico PLUS PI34577).
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bioRxiv - Cell Biology 2022Quote: ... 0.055 mM beta-mercaptoethanol (Gibco #21985023), 1x penicillin and streptomycin solution (Gibco #15140122) ...
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bioRxiv - Cell Biology 2022Quote: ... 0.055 mM beta-mercaptoethanol (Gibco #21985023), 1x penicillin and streptomycin solution (Gibco #15140122) ...
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bioRxiv - Cell Biology 2022Quote: ... 0.055 mM beta-mercaptoethanol (Gibco #21985023), 1x penicillin and streptomycin solution (Gibco #15140122) ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1 mM beta-mercaptoethanol (Gibco Co.), and hEGF 10 ng/mL (Gibco Co.) ...
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bioRxiv - Immunology 2022Quote: ... and anti-beta-actin antibody (Invitrogen).
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bioRxiv - Developmental Biology 2023Quote: ... 0.1% Beta-Mercaptoethanol (Gibco™, 21985023) with additional supplement of 8ng/ml LIF (MPI-CBG ...
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bioRxiv - Immunology 2023Quote: ... beta-mercaptoethanol (Gibco, 21-985-023) and penicillin-streptomycin ...
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bioRxiv - Immunology 2023Quote: ... 0,0003 % beta-mercaptoethanol (21985-023, Gibco) and 0,02 % beta-estradiol (Sigma).
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bioRxiv - Microbiology 2023Quote: ... and Beta-actin (Invitrogen #MA1-140). Secondary antibodies ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.1 mM beta-mercaptoethanol (all Gibco), SB431542 (10 μM ...
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bioRxiv - Molecular Biology 2024Quote: Anti-beta Tubulin (Invitrogen, PA1-16947)
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bioRxiv - Molecular Biology 2024Quote: ... Beta-actin (ThermoFisher Scientific, #MA5-15739) for overnight at 4℃ ...
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bioRxiv - Immunology 2024Quote: ... 1x Beta-mercaptoethanol (Gibco Cat#21985023), and additional cytokines depending on the T cell subsets being cultured.
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bioRxiv - Cancer Biology 2024Quote: ... beta-Actin (1:1000, Mouse, Invitrogen), Histone H3 (1:1000 ...
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bioRxiv - Systems Biology 2024Quote: ... , 10 uM beta-mercaptoethanol (Fisher Scientific), and 104 U/mL ESGRO LIF (Sigma Millipore) ...
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bioRxiv - Bioengineering 2024Quote: ... and beta mercaptoethanol (55 mM; Gibco). At about 14 days after the initiation of haematopoiesis ...
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bioRxiv - Microbiology 2024Quote: ... with specific primers and probes (Supplemental Table 1) and using a Quantstudio 3 (Applied Biosystems). The expressions of HCoV-229E M gene and SARS-CoV-2 E gene were quantified using a standard curve.
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bioRxiv - Developmental Biology 2021Quote: ... and the reverse primer containing the Sp6 promoter (5’ ATTTAGGTGACACTATAGCCTCTTCAGTTCCTTCTTCCATC-3’).The aqp1a.1 probe was generated from whole extracted cDNA at 30hpf and amplified with the primers (5’-GTCATGAACGAGCTGAAGAGC-3’) and (5’-GGGTCACTTTGAGGACATCTC-3’),incorporated into the pCR-BluntII-TOPO vector (Invitrogen, 45-0245) (containing both the SP6 and T7 promoters) ...
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bioRxiv - Microbiology 2021Quote: The mcr-1 gene was amplified from the clinical isolate ESBL20150072 (European Nucleotide Archive, accession number; SAMEA104060671) using primers: 5’-TTTTGGATCCCGGTTTTCGGGCTTTTTA-3’ and 5’-ATATGTCGACTCAGCGGATGAATGCGGT-3’ and Phusion polymerase (Thermo Fisher Scientific). PCR product was digested with BamHI and SalI and ligated into pACYC184 digested with the same enzymes (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies) and SYBR Select Master Mix (ThermoFisher, ABI 4472908).
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bioRxiv - Immunology 2022Quote: ... and hybridized first using RT primer (5′/5Biosg/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3′) and then reverse transcribed into cDNA using TSO primer (5′-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-3’) and RT maxima reverse transcription (Thermo Fisher Scientific). cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′ ...
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bioRxiv - Microbiology 2022Quote: ... The 16S rRNA gene was amplified with the primers 8f (5′-AGAGTTTGATCCTGGCTCAG-3′; Weisburg et al. 1991) and 1520 r (5′-AAGGAGGTGATCCAGCCGCA-3′; Edwards et al. 1989) (Invitrogen, CA, USA). A volume of 0.5 μL DNA extract was used for 50 μL PCR reactions containing 2 units Taq DNA polymerase ...
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bioRxiv - Plant Biology 2024Quote: ... The purified RNA was reverse transcribed using the CLIP-3 PCR primer (5’-GTCTCGTGGGCTCGG-3’) and SuperScript IV reverse transcriptase (18090010; Thermo Fisher Scientific). The first-strand cDNA was then subjected to PCR using the CLIP-5 PCR primer (5’-TCGTCGGGCAGCGTC-3’) ...
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bioRxiv - Molecular Biology 2024Quote: ... Expression of Kdm5c was detected us-ing the primers 5’-CCCATGGAGGCCAGAGAATAAG-3’ 5’-CTCAGCGGATAAGAGAATTTGCTAC-3’ and nor-malized to TBP using the primers 5’-TTCAGAGGATGCTCTAGGGAAGA-3’ 5’-CTGTGGAGTAAGTCCTGTGCC-3’ with the Power SYBR™ Green PCR Master Mix (ThermoFisher #4367659).
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bioRxiv - Microbiology 2022Quote: ... TGF-β and RANTES using custom ProcartaPlex assay kits (ThermoFisher Scientific). The analyte concentrations were determined using a Luminex 100/200 Flexmap3D instrument coupled with the Luminex XPONENT software.
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bioRxiv - Genomics 2022Quote: ... qPCR was performed with gene-specific primer probe fluorogenic exonuclease assays (TaqMan, Life Technologies, Waltham, MA, Supplemental table 5) and the QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... Targeted qPCR was performed using the QuantStudio 6 Real-Time PCR system using the following Taqman Primers (Applied Biosystems): BCL6 (Hs00153368_m1) ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was carried out using primers specific (Table S1) with SYBR Green Real-Time PCR Master Mixes (Invitrogen) and the StepOnePlus Real System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA and primers were used in a 20 μl qPCR reaction with PowerUp SYBR Green Master Mix (Applied Biosystems), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 μl of cDNA was added to 10 μl qPCR reactions containing 0.5 μM forward and reverse primers as well as 1X PowerUP SYBR green master mix (ThermoFisher) in 384-well plates ...
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bioRxiv - Cancer Biology 2020Quote: ... qPCR reactions were set up using the primers indicated in Supplementary Table 1 and POWRUP SYBR Master Mix (ThermoFisher). qPCR was performed on the QuantStudio6 real-time PCR system (Applied Biosystems).118,119
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bioRxiv - Genomics 2020Quote: ... These mixtures were measured in triplicate for each sample-primer combination with a ViiA 7 qPCR machine (Thermo Fisher)
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bioRxiv - Microbiology 2020Quote: ... a nested qPCR was performed using primers AA55M and L and the SYBR green master mix (Thermo Fisher Scientific).
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bioRxiv - Genomics 2023Quote: ... qPCR was performed with gene-specific primer probe fluorogenic exonuclease assays (TaqMan, Life Technologies, Waltham, MA, Additional file 1B) and the QuantStudio™ 12K Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed using TaqMan Fast Universal PCR mix and target (UTY) primer conjugated to FAM-probe (Hs01076483, ThermoFisher) with 18s rRNA primer (4319413E conjugated to VIC probe ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR was performed with gene-specific primer probe fluorogenic exonuclease assays (TaqMan, Life Technologies, Waltham, MA, Supplemental Table 2) on a QuantStudio 5 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2022Quote: ... and qPCR was performed with 200nM each of forward and reverse primers and PowerUp SYBR Green Master Mix (ThermoFisher) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized using oligo-dT and random hexamer primers according to the SYBR Green qPCR protocol (Life Technologies). Total RNA was reverse transcribed using High Capacity Reverse Transcriptase kits (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... quantitative PCR (qPCR) was performed using a gene-specific primer utilizing Powerup SYBR green master mix (Applied Biosystems # A25742). Fold change (2-ΔΔCt ...
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bioRxiv - Cell Biology 2023Quote: ... supplied with the primers for ICP27 and β-actin on a Quant Studio 6 Flex qPCR system (Applied Biosystems).
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bioRxiv - Microbiology 2023Quote: ... cDNA served as template in qPCR with gene-specific primers (S1 Table) and SYBR Green Master Mix (Applied Biosystems) using a StepOnePlus system according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR was carried out using sequence-specific primers and with PowerTrack SYBR green master mix (ThermoFisher, catalog number: A46109). Relative mRNA expression was calculated by the 2−ΔΔCT method of quantitative PCR after normalization with 18S rRNA levels ...
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bioRxiv - Bioengineering 2024Quote: ... GAPDH expression was quantified with a sensitive TaqMan-based qPCR assay using a commercial primer/probe set from ThermoFisher Scientific (Hs02758991_g1) ...
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bioRxiv - Cancer Biology 2024Quote: ... Real time qPCR was performed with primers and iQTM SYBER Green master mix on a QuantStudio 5 (Applied biosystems) by a standardized protocol ...
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bioRxiv - Immunology 2023Quote: ... post-dose 3 and 6-months post-dose 3 using mouse anti-human IgG1 biotin (Thermo Fisher Scientific) and mouse anti-human IgG4 biotin (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... 2 µl cDNA was amplified with TaqMan™ qPCR (38 cycles, 3 technical replicates of 3 biological replicates) using master mix (Thermo Fisher) and the following TaqMan assays ...
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bioRxiv - Biochemistry 2021Quote: ... We did this by amplification of the GlyR-FP plasmids using PCR with primers 5’-ATATGGTACCTGGGAGGTCTATATAAGCAGAG-3’ and 5’ATAAGGTACCCCAGGCGGGCCATTTACCGTA-3’ followed by digestion with KpnI (ThermoFisher Scientific, Merelbeke, Belgium) and ligation using instant sticky-end ligase Master mix (NEB ...