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Citations for Thermo Fisher :
501 - 550 of 602 citations for Fluorescein alkynylamino atp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... ATP was added to a final concentration of 1 mM along with 50 units of T4 ligase (ThermoFisher Scientific). The ligation reaction mixture was incubated at room temperature overnight ...
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bioRxiv - Biochemistry 2022Quote: ... ATP turnover was measured using an enzyme-coupled system41,42 and in specific cases the EnzChek Phosphate assay (Thermo Fisher) was also used to verify results (for tubulin and D-ring-stimulated CaKip3-MDN and CaKip3-MDNL2-HsKHC) ...
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bioRxiv - Cell Biology 2021Quote: ... reaction mixes were prepared using 2.5 μg recombinant His-ACBD5 with or without the addition of 0.3 mM ice-cold ATP (Thermo Fisher) and 0.1 ug GST-GSK3β (Abcam) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 5 pmol was 5’-end labeled with 20 μCi of Ψ-P32 ATP using Polynucleotide Kinase (Thermo Fisher) and subsequently purified using an Illustra MicroSpin G-50 column (GE Healthcare) ...
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bioRxiv - Pathology 2020Quote: ... by mixing 100 μL of lysate with 100 μL of D-luciferin (0,1 mg/mL) supplemented with ATP (0,5 M) in flat bottom 96-well black plates (Thermo Scientific). Luciferase activity was measured for 1 min with f/stop = 1 and binning = 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... recovered RNA was polyadenylated in the presence of ATP using 2400 U yeast poly(A) polymerase (yPAP, ThermoFisher Scientific) in 1x Poly(A ...
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bioRxiv - Plant Biology 2023Quote: ... Hybridization was performed using T4 PNK end labelled oligonucleotides with [γ-P32] ATP in hybridization buffer (Ultrahyb buffer-Invitrogen).
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bioRxiv - Plant Biology 2022Quote: ... cold ATP was used in the kinase assays at 200 μM and detection was using ProQ diamond stain (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ATP hydrolysis was analysed in steady state reactions using the EnzChek Phosphate Assay Kit (E-6646, Molecular Probes Inc.) according to the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2024Quote: ... Normalization of the ATP assays was done by Pierce BCA Protein assay kit (23227, Thermo Fisher Scientific Inc., Belgium). The following mitochondrial drugs were used ...
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bioRxiv - Biophysics 2024Quote: ... Cells were washed with the external patch-clamp buffer and incubated for 10 minutes with D-luciferin (0.5 μM) and luciferase (1.3 μg/mL) using ATP Determination Kit (ThermoFisher Scientific). Three minutes after the addition of LPC-16:0 (30 μM ...
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bioRxiv - Biochemistry 2024Quote: ... The reactions were initiated by adding 5mM ATP and terminated by the addition of LDS-NuPAGE loading buffer (Thermofisher). The samples were boiled at 95 °C for 10 minutes and stored at -80 °C if not processed immediately in immunoblot analysis ...
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bioRxiv - Genetics 2024Quote: ... after which 10 µL was mixed with 90 µL of ATP Determination reaction mixture (Cat. # A22066, Thermo-Fisher Scientific) and incubated at room temperature for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with fluorescein isothiocyanate-labeled goat anti-rabbit and anti-mouse secondary antibody (Alexa Fluor; Invitrogen; Thermo Fisher Scientific, Inc.). DAPI was applied to each section for 10 min to counterstain the nuclei.
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with fluorescein isothiocyanate-labeled goat anti-rabbit and anti-mouse secondary antibody (Alexa Fluor; Invitrogen; Thermo Fisher Scientific, Inc.). DAPI was applied to each section for 10 min to counterstain the nuclei.
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bioRxiv - Bioengineering 2022Quote: ... the protein of interest (10 μM) was incubated with a 10-fold molar excess of NHS-Fluorescein (Thermo Fisher Scientific, Cat: 46410) in PBS for 12 hours at room temperature and then purified through buffer exchange with the Amicon Ultra-0.5 centrifugal filters (10 kDa cutoff ...
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bioRxiv - Neuroscience 2022Quote: Mice were injected in the tail vein with 200 μL fluorescein isothiocyanate (FITC)-labeled dextran (Thermo Fisher Scientific, Waltham, MA, USA; D1820) prepared as a 25 mg/mL solution in 0.9% NaCl ...
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bioRxiv - Physiology 2021Quote: ... and/or 4-kDa fluorescein isothiocyanate (FITC) dextrans (TdB Consultancy, Uppsala, Sweden) and 30-50 µl of Hoechst 33342 (Invitrogen, Carlsbad, CA). Texas red-phalloidin (Invitrogen Corporation ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with the fluorescent lectin concanavalin A (ConA) (100 μg mL-1) conjugated to fluorescein isothiocyanate (FITC) (Invitrogen, United Kingdom) for 5 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... or 1 pmol of rif1-MO or 2 pmol of standard control MO together with a fluorescent tracer (dextran fluorescein lysine, Thermo Fisher Scientific) were microinjected into fertilized eggs ...
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bioRxiv - Developmental Biology 2023Quote: For the analysis of acrosome biogenesis peanut agglutinin (PNA)-fluorescein isothiocyanite (FITC) Alexa Fluor 488 conjugate (Molecular Probes, Invitrogen, Waltham, USA; L21409) was used on the Buin fixed testis tissues ...
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bioRxiv - Developmental Biology 2023Quote: For the analysis of acrosome biogenesis peanut agglutinin (PNA)-fluorescein isothiocyanite (FITC) Alexa Fluor 488 conjugate (Molecular Probes, Invitrogen, Waltham, USA; L21409) was used on the Buin fixed testis tissues ...
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bioRxiv - Pathology 2023Quote: ... were incubated with 20 μg/ml DQ gelatin fluorescein conjugate for 2 h at 37°C according to kit instructions (EnzChek Gelatinase/Collagenase Assay Kit, E-12055, Molecular Probes, Inc). The fluorescence generated by hydrolysis of the added substrate was captured by ZEISS Axioscan 7 (ZEISS Microscopy ...
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bioRxiv - Cell Biology 2023Quote: The SUMO-CCM2(413-438) contains a single cysteine (C437) that was used to couple the fusion protein with a maleimide-fluorescein dye (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... To differentiate the location of barcoded arrays in the fluorescent image, fluorescein isothiocyanate (FITC, 488 nm emission) conjugated bovine serum albumin (BSA, 1 mg/ml, Thermo Fisher Scientific) in 1x PBS was introduced to the first microfluidic channel ...
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bioRxiv - Cell Biology 2024Quote: ... two different probes were used: chloromethyl derivative of di-chloro-fluorescein diacetate (CM-DCFDA) at a concentration of 10 µM (Invitrogen, Life Technologies), and dihydroethidium (DHE ...
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bioRxiv - Molecular Biology 2020Quote: ... Media was collected following in vitro models of VILI and extracellular ATP was measured using a luciferase-luciferin assay (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... and 0.3 mM ATP were reacted with a 10-fold molar excess of N-(1-pyrene)iodoacetamide (Thermo Fisher Scientific) dissolved in dimethylformamide (DMF ...
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bioRxiv - Molecular Biology 2021Quote: ... in 10 mM Tris pH7 for ATP in a poly(A)-tailing reaction with a Poly(A) Tailing Kit (Ambion) as described above ...
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bioRxiv - Bioengineering 2021Quote: ... The concentration of the free ATP in the dilute phase was evaluated by measuring its absorbance at 259 nm using the molar extinction coefficient of ATP (15,400 M-1 cm-1) using UV-Vis absorption spectroscopy (NanoDrop 2000/2000c Spectrophotometer, Thermo Scientific). The concentration of ATP inside the coacervates was calculated as cdense = (c − cdilutef)/(1 − f) ...
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bioRxiv - Biochemistry 2020Quote: ... All the SAM with natural isotope abundance and 2H or 13C enrichment were enzymatically synthesized from L-methionine and ATP with appropriate isotope labels. Escherichia coli (E. coli) DH5α and BL21(DE3) competent cells were from Invitrogen. Data plotting and nonlinear least-squares fitting was carried out using GraphPad Prism 7 software ...
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bioRxiv - Plant Biology 2020Quote: ... 60-mer DNA oligonucletoides were 5’ end-labeled using [γ-32P]-ATP and PNK (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... A third aliquot was diluted 1:1000 with 25 mM Tris-acetate pH 7.75 and ATP measured using luciferin-luciferase reagent (Invitrogen, #A22066). Throughout the extraction ...
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bioRxiv - Pathology 2023Quote: ... The aqueous phase was diluted 1:1000 with 25 mM Tris-acetate buffer pH 7.75 and ATP measured using luciferin-luciferase reagent (Invitrogen, #A22066). Background was estimated by omission of the incubation step.
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bioRxiv - Molecular Biology 2024Quote: ... Beads containing GFP-tagged targets were distributed evenly in kinase reaction tubes and resuspended in 50 μL 1x Kinase Buffer containing 1 mM ATP (ThermoFisher) with or without 0.2 μM of non-phosphorylated Avi-GSK-3βS9A and 0.2 μM His6-STAT3127-715 (Panagi et al. ...
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bioRxiv - Cell Biology 2024Quote: Quantitative estimation of total ATP in C2C12 myotubes was performed using the luciferin-luciferase bioluminescence assay (#A22066, Thermo Scientific, US). Myotubes were collected using trypsin (#12604013 ...
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bioRxiv - Plant Biology 2024Quote: ... The probe was 5’-end labeled with [γ-32P]-ATP and T4 Polynucleotide Kinase (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Kinase assay was then performed by incubating with 0.5mM ATP and Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher) for 60min at 30°C on a shaking heat block ...
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bioRxiv - Synthetic Biology 2024Quote: ... splint oligo DNA No.1 (0.05nmol) in a total reaction volume of 50µl (in the presence of PEG4000, ATP and T4 DNA Ligase Thermo Scientific). The 25nt RNA was additionally 5’-phosphorylated (T4 PNK 10U/µl NEB ...
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bioRxiv - Bioengineering 2021Quote: ... was added to a well plate (below inserts) and 100 μL of 100 μg/mL of fluorescein isocyanate (FITC)-labeled bovine serum albumin (FITC-BSA, Thermo Fisher Scientific, USA) was added into an insert and incubated at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... a solution containing the synthesized Kaede-NLS mRNA at a concentration of 200 ng/µl and fluorescein isothiocyanate (FITC) from Thermo Fisher (Catalog #46425) was injected into fertilized eggs ...
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bioRxiv - Biochemistry 2022Quote: ... A 2-fold excess of BODIPY-FL C5-maleimide (FN3-AFF samples) or Fluorescein 5 maleimide (FN3 and cpFN3 samples) (ThermoFisher Scientific, Waltham MA) was immediately added and the reaction was allowed to proceed for 1 h at room temperature or overnight at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were carried out in PLS buffer supplemented with 0.2 mM ATP (when actin was present) and SYPRO Orange dye (1x final concentration; Invitrogen, Carlsbad, CA). Plastin constructs were added to a final concentration of 5 μM ...
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bioRxiv - Developmental Biology 2020Quote: ... The amount of ATP was then determined and normalized with the protein level measured by BCA protein assay (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... the pooled anion exchange fractions were phosphorylated in vitro overnight at 4 °C with 1 mM ATP and 1 μg of PKR(252-551)-GST enzyme (Thermo Scientific) per mg of eIF2α ...
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bioRxiv - Microbiology 2021Quote: ... ATP-y-S incubated and SFX or dibucaine incubated samples was performed using a 200 kV Talos Arctica (Thermo Fisher Scientific) equipped with a Gatan K2 Summit direct detector ...
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bioRxiv - Molecular Biology 2022Quote: ... intracellular ATP levels were determined by reaction of ATP with recombinant firefly luciferase and its substrate D-luciferin according to the manufacturer’s instructions (Thermo Scientific, A22066).
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bioRxiv - Immunology 2020Quote: The following fluorescent reporters were added to the cell cultures 15 mn before ATP stimulation: Hoechst 33342 (10 μg/mL) (Invitrogen, H3570), LysoTracker Green DND-26 (1 μM ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were diluted 1:100 in extraction buffer before quantification using a luciferase-based ATP kit (A22066, Thermo Fisher Scientific). Values were expressed relative to total protein concentration measured by using a BCA assay (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... The ATP content from 10 μL aliquots of lysed cells was then detected using a luminescence-based ATP determination kit from Life Technologies and ATP levels were calculated by extrapolation from a calibration curve constructed with known concentrations of ATP ...