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Citations for Thermo Fisher :
451 - 500 of 602 citations for Fluorescein alkynylamino atp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were incubated with biotinylated RBD, together with anti-c-Myc fluorescein isothiocyanate (FITC, Miltenyi Biotech) and streptavidinphycoerythrin (SAPE, ThermoFisher). The concentration of SAPE in the with-avidity method was used at ¼ concentration of the biotinylated RBD ...
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bioRxiv - Bioengineering 2021Quote: ... the mouse Achilles tendon samples were stained with 0.2% of 4 mg/ml fluorescein diacetate (FDA) (596-09-8, ACROS Organics) and 0.5% of 1 mg/ml propidium iodide (PI ...
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bioRxiv - Molecular Biology 2022Quote: ... FAM-GreenB1 or FAM-RND was detected using a 1:200 dilution of rabbit anti-fluorescein antibody (#A-11090, ThermoFisher) at RT for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-mouse or anti-rat or anti-rabbit fluorescein-conjugated secondary antibodies (dilution 1:500, Invitrogen) and cell nuclei were stained with DAPI (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... the supernatant of human coronary artery smooth muscle cells overexpressing ADAMTS7 or a mock plasmid was used with fluorescein conjugates of gelatine (DQ Gelatine from Pig Skin, ThermoFisher). Supernatants were plated on a flat black 96-well plate (Nunclon ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were incubated for 1 h in the dark with a secondary antibody conjugated to fluorescein isothiocyanate (FITC; Invitrogen). Next ...
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bioRxiv - Neuroscience 2023Quote: ... The drill was then replaced with a micropipette loaded with 3-5nL of 0.6 M MnCl2 with 0.3mg/ml 3K rhodamine dextran amine (RDti and 0.15mg/ml 10K fluorescein dextran amine (Molecular Probes/Invitrogen/ThermoFisher) in sterile phosphate-buffered saline ...
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bioRxiv - Synthetic Biology 2023Quote: ... the cells were incubated with biotinylated target, together with anti-c-Myc fluorescein isothiocyanate (FITC, Miltenyi Biotech) and streptavidin–phycoerythrin (SAPE, ThermoFisher). The concentration of SAPE in the with-avidity method was used at one-quarter of the concentration of the biotinylated targets ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were incubated with biotinylated Hsc70, together with anti-c-Myc fluorescein isothiocyanate (FITC, Miltenyi Biotech) and streptavidin–phycoerythrin (SAPE, ThermoFisher). The concentration of SAPE in the with-avidity method was used at 1/4 concentration of the biotinylated Hsc70 ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... The second antibody was then added in PBS 1X and 5% goat serum 1h at ambient temperature (dilution 1/200, Alexa FluorTM 488 goat anti-mouse IgG (AF488, molecular probes) or fluorescein goat anti-rabbit IgG (FITC, Invitrogen)) ...
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bioRxiv - Neuroscience 2024Quote: ... was labeled via retro-orbital injection of fluorescein (25mg of dextran conjugate dissolved in 200 mL of sterile saline, 70-kDa molecular weight; D1823, Invitrogen).
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bioRxiv - Evolutionary Biology 2024Quote: ... 300 µL of dense rosette cultures were first stained for ECM with fluorescein-jacalin (Eurobio Scientific FL-1151-5) and for membranes with FM 4-64 (Invitrogen T13320 ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were incubated with biotinylated target, together with anti-c-Myc fluorescein isothiocyanate (FITC, Miltenyi Biotech) and streptavidin–phycoerythrin (SAPE, ThermoFisher). The concentration of SAPE in the with-avidity method was used at one-quarter of the concentration of the biotinylated targets ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were incubated with biotinylated target, together with anti-c-Myc fluorescein isothiocyanate (FITC, Miltenyi Biotech) and streptavidin–phycoerythrin (SAPE, ThermoFisher). The concentration of SAPE in the with-avidity method was used at 1/4 concentration of the biotinylated target ...
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bioRxiv - Genomics 2020Quote: ... Here, 40 μL pre-mix (5 μL 10x NEBuffer 2.1, 2 μL 100 mM ATP (Thermo Fisher, R0441), 3 μL 100 mM DTT ...
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bioRxiv - Biophysics 2022Quote: Glycerol in the ATP synthase preparation was removed with a Zeba Spin desalting column (Thermo Fisher Scientific [TFS]) before freezing cryoEM specimens ...
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bioRxiv - Immunology 2022Quote: ... primed cells were washed and then activated with 5 mM ATP diluted in Opti-MEM media (Thermo Fisher) for 60 min and supernatants collected.
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was diluted 1:100 and luminescence was monitored using an ATP determination kit (A22066, Molecular Probes). Luminescence readings for each sample were compared to an ATP standard curve and normalized to the total amount of protein determined using the Pierce BCA Protein Assay Kit (23225 ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting reaction mixtures then were supplemented with 1 μl 0 or 1 mM ATP (>99% purity; ThermoFisher) in transcription buffer and incubated 5 min at 37°C (to enable termination) ...
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bioRxiv - Neuroscience 2024Quote: ... Daily working solutions were diluted to 100 µM ATP in aCSF with 15 µg/mL Alexa568 (Invitrogen A33081) to visualize the puff region ...
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bioRxiv - Plant Biology 2023Quote: ... 0.5 mM ATP and the restriction enzymes in 1x Fast Digest buffer (Thermo Fisher Scientific Inc., Waltham, USA). The reaction mixture was incubated for 1 min at 37 °C and 1 min at 16 °C for in total 30 cycles ...
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bioRxiv - Plant Biology 2023Quote: ... DNA oligonucleotides complementary to sRNA and U6 were end- labeled with [γ-32P]ATP using T4 PNK (ThermoFisher). Membranes were incubated at 42 °C with PerfectHyb Plus hybridization buffer (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and the Molecular PROBes® ATP determination Kit as described by the manufacturer (Life Technologies, Burlington, ON, Canada). ATP was quantified in replicates of 6 using the Synergy H4 Hybrid Multi-well Plate Reader (Biotek ...
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bioRxiv - Physiology 2024Quote: ... 25 μM ATP and the PmFB to 300 μl CRC buffer comprising 1 μM Calcium Green-5N (Invitrogen), 20 mM creatine ...
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bioRxiv - Bioengineering 2020Quote: The barrier permeability function of the transport between the fetal and maternal microchannels was analyzed using 3000 MW fluorescein-dextran anionic probes (Invitrogen, ThermoFisher), as described previously.21 The Fluorescein-dextran was initially diluted in PBS to 100 mg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were incubated in PBT plus 2% normal goat serum with primary antibodies at the following dilutions: rabbit anti-fluorescein (ThermoFisher #A889) 1:500 and mouse anti-Drosophila Brp (nc82 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... including Alexa 555-conjugated ovalbumin (45 kDa) and Fluorescein 3000 MW Anionic dextran (3 kDa) were purchased from Invitrogen (CA, USA). D-Amphetamine (14204 ...
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bioRxiv - Cancer Biology 2022Quote: ... was determined by measuring the phosphorylation of a FRET peptide substrate (Ser/Thr19) labeled with Coumarin and Fluorescein using Z’-LYTE™ assay (Invitrogen). The 10 µL assay mixtures contained 50 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2022Quote: ... The sections were then incubated with the corresponding fluorescein-conjugated secondary antibodies (Alexa Fluor 488 anti-rabbit, Invitrogen A11008, 1:1000) for 2 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The redox homeostasis of promastigotes was monitored both qualitatively and quantitively by 2-7 dichlorodihydro fluorescein diacetate (DCFDA) (Life Technologies, USA) staining ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was carried out in a final volume of 20 μl using Maxima SYBR green/fluorescein qPCR (2x) Master Mix (Thermo Scientific). The reaction was performed in a C1000 thermal cycler (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were double-blinded microinjected into the cytoplasm of single COS-7 cells (n=100-200 cells per sample) along with fluorescein-labeled dextran (molecular weight 10000, Life Technologies) using a micromanipulator (Narishige) ...
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bioRxiv - Microbiology 2022Quote: Fluorescein-labeled 300 base β-actin-Mouse was synthesized using MAXIscript T7 Transcription Kit (AM1312, Invitrogen by Fisher Scientific, Waltham, MA) and Fluorescein RNA Labeling Mix (11685619910 ...
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bioRxiv - Microbiology 2022Quote: Fluorescein-labeled 300 base β-actin-Mouse was synthesized using MAXIscript T7 Transcription Kit (AM1312, Invitrogen by Fisher Scientific, Waltham, MA) and Fluorescein RNA Labeling Mix (11685619910 ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR analysis of DNA and cDNA was performed with Maxima SYBR Green/Fluorescein qRT-PCR Master Mix (Thermo Fisher Scientific) using the CFX Connect Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... fluorescence was observed under a confocal microscope (LSM880NLO, Zeiss, Germany) using 2’,7’-dichlorodihydro fluorescein diacetate (H2DCFDA, 10 InvM, Invitrogen, USA) as described by Yu et al ...
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bioRxiv - Cell Biology 2024Quote: ... The sections were washed with PBS and then incubated with a reaction buffer containing 25 µg/mL fluorescein-conjugated DQ gelatin (D12054, ThermoFisher Scientific) for 18 hours at room temperature in a dark ...
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bioRxiv - Microbiology 2023Quote: ... was added at a final concentration of 10 μg/mL and the polysaccharide stain fluorescein-conjugated wheat germ agglutinin (WGA) (Invitrogen™) was added at a final concentration of 10 μg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... and then received 0.1mL of either 1.5-3% Fluorescein isothiocyanate–dextran (FITC) or Texas Red dextran (70kDa, Sigma-Aldrich #46945 and Thermofisher D1830, respectively), to permit visualization of the cerebral vasculature ...
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bioRxiv - Bioengineering 2023Quote: ... we add 50 μg/mL fluorescein isothiocyanate (FITC)-labeled dextran with a molecular weight of 70 kDa (Invitrogen, Cat. No. D1823) to the reaction mixture ...
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bioRxiv - Bioengineering 2023Quote: ... were incubated with FBS-free RPMI-1640 medium supplemented with 10 μg/mL of fluorescein isothiocyanate-conjugated ovalbumin (FITC-ovalbumin; Invitrogen, Germany) for 1 hour under standard cell culture conditions ...
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bioRxiv - Bioengineering 2023Quote: ... Experiments to analyze barrier permeability after collagenase exposure included luminal media with 0.1% 10kDa dextran fluorescein (Thermo Fisher Scientific, Waltham, WA) for only the last 2 h of the experiment.
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bioRxiv - Neuroscience 2023Quote: ... 17-mon old SrfGFAP-ERCKO (15-mon post-Tam injection) mice were deeply anesthetized with isoflurane and injected with 20 µl of 10 kDa dextran fluorescein (Invitrogen; #D1820) into the left cardiac ventricle ...
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bioRxiv - Neuroscience 2023Quote: ... The drill was then replaced with a micropipette loaded with 3-5nL of 0.6 M MnCl2 with 0.3mg/ml 3K rhodamine dextran amine (RDti and 0.15mg/ml 10K fluorescein dextran amine (Molecular Probes/Invitrogen/ThermoFisher) in sterile phosphate-buffered saline ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections were then incubated for 60 min at RT with wheat germ agglutinin (WGA) conjugated with fluorescein isothiocyanate or rhodamine (ThermoFisher, USA) and diluted 1:1000 in PBS with 0.1% BSA ...
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bioRxiv - Bioengineering 2024Quote: ... BMDMs and differentiated CIMs were detached from Petri dishes with TrypLE Express and labeled with CellTracker Deep Red or Vybrant carboxy- fluorescein diacetate succinimidyl ester Cell Tracer (Invitrogen V12883) as described above ...
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bioRxiv - Cell Biology 2024Quote: ... two different probes were used: chloromethyl derivative of di-chloro-fluorescein diacetate (CM-DCFDA) at a concentration of 10 µM (Invitrogen, Life Technologies), and dihydroethidium (DHE ...
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bioRxiv - Cell Biology 2024Quote: ... EBSS was then replaced with Neurobasal A media with 33 μg/ml fluorescein isothiocyanate (FITC)-conjugated transferrin (Thermo Fisher Scientific #T2871), which is pH sensitive ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 mM ATP) and then stained with a far-red fluorescent membrane dye (cat no. C10046; Thermo Fisher). Prior to the cell stiffness assay ...
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bioRxiv - Cancer Biology 2020Quote: ... Proton transport was measured using ATP-dependent quenching of 9-amino-6-chloro-2-methoxy-acridine (Acridine Orange, ThermoFisher) fluorescence quenching for isolated vacuoles as previously described21