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Citations for Thermo Fisher :
501 - 550 of 653 citations for Cyclosporin A 13C2 d4 Acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... All samples were boiled for 5 min prior to separation by SDS PAGE on precast 3-8% Tris-Acetate NuPAGE gels (Invitrogen). SDS-PAGE fractionated proteins were transferred to a nitrocellulose membrane using a semidry Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Biochemistry 2023Quote: ... at 70 °C for 7 min then precipitated in 75% ethanol with 10% volume of sodium acetate (3 M, pH 5.5) and GlycoBlue (1 μL, Invitrogen AM9515). The RNA fragments were dissolved in nuclease-free H2O at a concentration of 100 ng/μL ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2023Quote: ... Dephosphorylated mRNA fragments were precipitated in 100% ethanol with 40 μl 3 M sodium acetate and 1 μl linear acrylamide (AM9520, Ambion) at -20°C for >16 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was precipitated by transferring 320 μl of supernatant to a new RNase-free microcentrifuge tube and adding 32 μl of 3 M sodium acetate (pH 5.5) (Invitrogen AM9740), 2.5 μl of RNA-grade glycogen (Thermo Scientific R0551) ...
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bioRxiv - Cell Biology 2024Quote: ... 30 to 50 μg of protein extracts were resolved by SDS-polyacrylamide gel electrophoresis using 4–12% NuPAGE Bis-Tris gradient gel or 3–8% Tris-acetate gel and run (Invitrogen) and MOPS or Tris-acetate running buffer (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... a solution of 1 × 106 cells/ml supplemented with 5 ng/ml phorbol 12-myristate 13-acetate (PMA, ThermoFisher Scientific) was seeded in each well of a 12-well cell culture plate (TPP ...
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bioRxiv - Biochemistry 2024Quote: ... Excess solution was dabbed away and the grids were negative stained with 10 uL of 5% uranyl acetate (ThermoFisher Scientific) for 5 minutes before excess solution was dabbed away ...
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bioRxiv - Genomics 2024Quote: ... was prepared fresh each time by measuring NaIO4 powder and resuspending to a concentration of 4 mg/ml in aqueous solution of 200 mM sodium acetate (pH 5.5) (Invitrogen, AM9740). Input RNA (up to 5 μg ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were run on a Criterion XT 3-8% Tris-Acetate PAGE gel in 1x XT Tricine and analysed by silver staining (SilverQuest, Invitrogen) or immunoblotting ...
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bioRxiv - Cell Biology 2024Quote: ... The aqueous layer was transferred into a new tube and the RNA was precipitated by adding 3 M sodium acetate pH 5.2 (Thermo Scientific), glycoblue (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... Proteins were loaded and run on a 4-12% Bis-Tris pre-cast gel or a 3-8% Tris-Acetate pre-cast gel (Invitrogen) and were electrophoretically transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Biophysics 2021Quote: ... Unreacted amine groups on the silane molecules were capped using NHS-sulfo acetate (10 mg/ml for 30 mins; Thermo Fisher). PEG-functionalized AFM cantilevers were incubated in 1 mg/ml Bovine Serum Albumin 55 overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples of SMG6-3xFLAG in vitro translation reactions were run on 3-8% Tris-Acetate NuPAGE gel (Invitrogen, Cat. No EA03752BOX). Transfer onto nitrocellulose membranes was performed using the iBlot 2 Dry Blotting System (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... For MS of permethylated glycolipids in positive ion mode,∼0.4 nmol of permethylated total glycolipids were dissolved in 50 µl of 1 mM sodium acetate in methanol/water (1:1) for infusion into a linear ion trap mass spectrometer (Orbi-LTQ; ThermoFisher Scientific) using a nanospray source at a syringe flow rate of 0.40 µl/min and capillary temperature set to 210 °C [18–21] ...
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bioRxiv - Immunology 2021Quote: ... and checked for purity and quality on a silver-stained SDS-PAGE gel (3-8% Tris-Acetate Novex, Thermo Fisher Scientific). The anti-NTD neutralizing antibody NTD-18 and the pan-coronavirus anti-S2 non-neutralizing antibody Ab-10 were previously described 9,10.
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bioRxiv - Genomics 2020Quote: ... 1% Triton X-100 and 10% glycerol and resolved on 3–8% pre-cast gradient Tris-Acetate gels (Invitrogen, Waltham, MA). For mouse samples ...
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bioRxiv - Biophysics 2021Quote: ... according to the manufacturer’s protocol and then purified using one round of each phenol/chloroform and chloroform extraction followed by precipitation using ethanol in presence of 300 mM sodium acetate pH 5.5 and GlycoBlue coprecipitant (Thermo Fisher Scientific). RNA concentration and purity were determined by absorbance measurement ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg protein/lane was then separated under denaturing conditions on reducing 3~8% Tris-Acetate gels (NuPAGE, Thermo Fisher Scientific), and HiMark protein standard (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were denatured by adding 4xLDS buffer and heating at 70°C for 10 min before being separated in 3-8% Tris-acetate gels (Thermo Fisher) run at 150 V for 3 hours ...
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bioRxiv - Bioengineering 2021Quote: ... 1.6 μm particle size) at the flow rate of 150 μl min-1 under a gradient of ammonium acetate and acetonitrile (Fisher Scientific). Multiple reaction monitoring (MRM ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then resolved on 4-12% Bis-Tris gels (for lower molecular weight proteins) or 3-8% Tris-Acetate gels (for higher molecular weight proteins) (NuPage, Thermo-Fisher Scientific) and run in MOPS or Tris-Acetate running buffer respectively ...
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bioRxiv - Bioengineering 2020Quote: ... Cisapride and norcisapride were further separated by a gradient mode of 10 mM ammonium acetate-acetonitrile as mobile phase using Acclaim Trinity P1 reverse phase column (2.1 × 50mm, 3 μm; Thermo Fisher Scientific). Mass ion transitions (Q1/Q3 ...
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Molecular Biology 2022Quote: Protein extracts were resolved using either home-made SDS-PAGE gels or 3-8% NuPAGE Tris-Acetate gels (Thermo Fisher Scientific), when analysing proteins of a molecular weight >180 kDa ...
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bioRxiv - Microbiology 2022Quote: ... stained by uranyl acetate 2% in H2O for 10 min and examined at 100kV by EM 208S Transmission Electron Microscope (FEI - Thermo Fisher) equipped with the acquisition system Megaview SIS camera (Olympus).
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bioRxiv - Pathology 2022Quote: ... Protein concentration was determined with Bio-Rad Protein Assay Kit II and 15 μg sample protein was loaded in a NuPAGE 3-8 % Tris-Acetate gel (Thermo Fisher). The gel was run at a constant voltage of 150 V for 4 h ...
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bioRxiv - Biophysics 2022Quote: ... Purified surface proteins or total cell lysates were separated on a NuPage 3-8 % Tris-acetate protein gel (Thermo Fisher Scientific) at 200 V for 40 min and transferred to a PVDF membrane ...
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bioRxiv - Biochemistry 2022Quote: ... All ssDNAs were dialyzed into 100 mM ammonium acetate with Pierce 96-well microdialysis devices with 3.5K MWCO (Thermo Fisher Scientific). For preparation of LiRecT-DNA complexes ...
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bioRxiv - Immunology 2022Quote: ... The quality/purity of the different purified fractions was evaluated by SDS-PAGE using 3–8% Tris– Acetate gels (Life Technologies) under non-reducing conditions followed by silver staining (Silver Stain kit ...
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bioRxiv - Cell Biology 2021Quote: ... Protein lysates were separated on tris-glycine or tris-acetate SDS-PAGE gels and transferred to a nitrocellulose membrane using the iBlot 2 dry blotting system (Invitrogen IB21001). The following primary antibodies where used for western-blotting at 1/1000 dilution ...
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bioRxiv - Systems Biology 2021Quote: ... The final transformation mixture contained 10 mM LiAc (Lithium acetate dihydrate, 98%, extra pure, ACROS Organics™, CAS 6108-17-4), 50 µg ssDNA (UltraPure™ Salmon Sperm DNA Solution ...
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bioRxiv - Genomics 2021Quote: ... Purified DNA was eluted with 4 μL of 10 mM Tris-acetate (pH 8.0) and analyzed on a 10% Novex TBE-Urea gel (Thermo Fisher Scientific). Fluorescent images were obtained using Typhoon Trio+ and analyzed with ImageQuant software (GE Healthcare).
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were passed through a 25G syringe five times to reduce viscosity and separated on a 3%-8% NuPAGE Tris-Acetate gel (Thermo Fisher). Proteins were transferred onto a polyvinylidene fluoride membrane (Thermo Scientific ...
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bioRxiv - Genomics 2020Quote: ... An estimated 60 µg of protein was loaded per well on a NuPAGE Novex 3-8 % Tris-Acetate Protein gel (Life Technologies), separated by electrophoresis and blotted on polyvinylidene fluoride membranes ...
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bioRxiv - Developmental Biology 2020Quote: ... For the detection of Gli3 10μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... The grid was stained with 2% uranyl acetate and air-dried before loading to a Talos L120C microscope (Thermo Fisher Scientific). The length of the array was directly measured in the ImageJ and converted to the equivalent number of OADs for subsequent quantitative analysis.
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bioRxiv - Microbiology 2023Quote: ... proteins were crashed out by addition of 300 µl ethyl acetate and all samples were evaporated to dryness in a SpeedVac vacuum concentrator (SPD131DDA SpeedVac Concentrator; Thermo Scientific). Dried samples were stored at −20C until further processing ...
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bioRxiv - Molecular Biology 2023Quote: DNA illustration by agarose gel electrophoresis has been conducted by exploiting 2% agarose gel and 1X TAE buffer (1 mM EDTA and 40 mM Tris-acetate, Fisher Scientific) and by applying constant voltage of 120 V for 40 to 60 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Microbiology 2023Quote: High-performance LC grade solvents (water, acetonitrile, chloroform, and methanol) and ammonium acetate (Optima LC/MS grade) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cultures were stimulated with phorbol 12-myristate 13-acetate (PMA) (5 ng/mL, Alfa-Aesar) + Ionomycin (0.5 µg/mL, Thermo Fisher Scientific) and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... all samples were buffer exchanged to 200 mM ammonium acetate with Zeba™ spin desalting columns (Thermo Fisher Scientific, Waltham, MA), with an approximate protein concentration of 2-10 µM ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was eluted in 50 μl water and precipitated with 0.75 M ammonium-acetate and 10 μg glycogen (ThermoFisher, Cat# R0551) by adding 125 μl ethanol (100 %) ...
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bioRxiv - Microbiology 2022Quote: ... Stool supernatant from the primary collection were obtained by centrifugation and filtration (0.2 μM surfactant-free cellulose acetate; Thermo Scientific Nalgene). Proteins were precipitated (1:7 ratio (v/v ...
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bioRxiv - Immunology 2022Quote: ... THP1 cells were differentiated into macrophage-like cells by treatment with 10nM phorbol 12-myristate 13-acetate (PMA) for a period of 72h. Lipofectamine2000 (cat no. #11668019) from Thermo Fisher Scientific or Helix-IN reagent (cat no ...