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Citations for Thermo Fisher :
451 - 500 of 653 citations for Cyclosporin A 13C2 d4 Acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... acetonitrile/water (95/5) with 10 mM ammonium acetate (pH 8.2) (All solvents were LC–MS Optima grade from Fisher Scientific). The total run time was 11 min ...
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Cancer Biology 2020Quote: ... ATM and ATR in the CE samples as follows: the samples were loaded on 4-8% (w/v) Tris-acetate SDS-PAGE gels (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were resolved on NuPage 3-8% w/v Tris-Acetate or 4-12% w/v Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2021Quote: ... the total amount of PCR product was separated by electrophoresis at 110 V for 40 minutes in 1.8% agarose gel diluted in 1 × Tris-acetate EDTA buffer (w/v) and stained with 0.01% of SYBR Safe (Thermo Fisher).
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bioRxiv - Cell Biology 2021Quote: Extracts were analyzed by SDS–PAGE using a 4-12% Bis–Tris or 3-8% Tris-Acetate NuPAGE gels (Invitrogen) and transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... the buffer was exchanged for the 0.1 M ammonium acetate using a Zeba column 40K (Thermo Fisher Scientific, Waltham, Massachusetts), wash steps completed per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... The DNA in aqueous phase was precipitated with sodium acetate and ethanol along with 1 μl glycogen carrier (Thermo Scientific) overnight at −20°C and then centrifuged to pellet at 4°C (12,000 g ...
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bioRxiv - Neuroscience 2020Quote: ... Gels were run in 1X MOPS or 1X tris-acetate SDS running buffer containing 500 μL of antioxidant (Life technologies) for 90 min ...
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bioRxiv - Molecular Biology 2020Quote: ... all the enzymes were buffer exchanged against 1 mol·L−1 ammonium acetate at pH 7.5 using microcentrifuge gel-filtration columns (Zeba 0.5 mL, Thermo Scientific, Rockford, IL). Protein concentration was determined with a NanoDrop™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclease S1 cleavage was carried out for 15 minutes at room temperature in 1× Reaction Buffer (40 mM sodium acetate pH 4.5, 300 mM NaCl and 2 mM ZnSO4; Thermo Scientific), and 0.1 μg of total yeast tRNA (Ambion ...
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bioRxiv - Cell Biology 2021Quote: ... and equal concentrations of each sample were separated on a 3-8% Tris-Acetate or 4-12% Bis-Tris gel (Invitrogen) by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Genomics 2022Quote: ... is prepared fresh each time by measuring NaIO4 powder and resuspending to a concentration of 4 mg/mL in aqueous solution of 200 mM Sodium Acetate pH 5.5 (Invitrogen, AM9740). Input RNA (up to 5 µg ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... Separation of an equal amount of proteins by SDS-PAGE was performed using 4-12% NuPAGE Tris-Acetate gels (Invitrogen). Subsequently ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were boiled for 5min at 95°C before being loaded on a 3-8% NuPAGE Tris-Acetate gel (Invitrogen). Western blots were quantified using ImageJ ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were heated to 70°C and loaded on 3-8% Tris-Acetate Protein Gels (ThermoFisher Scientific, Waltham, MA) or NuPAGE 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... followed by buffer exchange using the 0.5 ml centrifugal filter described above (cat. no. UFC503024, MilliporeSigma) and 10 mM ammonium acetate buffer (cat. no. AM9070G, Invitrogen).
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bioRxiv - Genetics 2022Quote: ... in 900 uL of 50 mM sodium acetate (pH 124 = 5.5) containing 1X Halt protease inhibitor cocktail (Thermo Fisher Scientific). The samples were centrifuged at 14.000 g for 20 min at 4°C to pellet particulates ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of samples (50-100 µg) were resolved by SDS-polyacrylamide gel electrophoresis in 3–8% Tris-Acetate gels (Thermofisher). Gels were wet-transferred in 1x NuPAGE Transfer Buffer (Thermofisher ...
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bioRxiv - Cell Biology 2022Quote: ... 8% Tris-glycine gels at 150 volts for 2.25 hr or pre-poured NuPAGE 3-8% Tris-Acetate gel (Invitrogen; EA03752) for 2.15 hr (Figure 1C ...
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bioRxiv - Cell Biology 2020Quote: ... the buffer was exchanged for the 0.1 M ammonium acetate using a Zeba column 40K (Thermo Fisher Scientific, Waltham, Massachusetts), wash steps completed per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated in 4-12% Nu-Page Bis-Tris (FANCA analysis) or NuPAGE 3-8% Tris-Acetate (FANCD2) polyacrylamide gels (Invitrogen) and transferred to nitrocellulose membranes (Amersham Biotech ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were run at 200V for an hour through either a 4-12% Bis-Tris or 3-8% Tris Acetate gel (Invitrogen), and then transferred at 300 mA for one hour in 15% methanol 1x Transfer Buffer on a methanol activated PVDF membrane ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cellular proteins fractionated through 3-8% polyacrylamide Tris-acetate or 4-12% polyacrylamide Bis-Tris gels (Invitrogen) were subsequently transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Pathology 2023Quote: ... A third aliquot was diluted 1:1000 with 25 mM Tris-acetate pH 7.75 and ATP measured using luciferin-luciferase reagent (Invitrogen, #A22066). Throughout the extraction ...
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bioRxiv - Physiology 2022Quote: ... and rinsed in deionized (DI) H2O prior to en bloc staining with 2% (w/v) uranyl acetate (Thermo Fisher Scientific). After dehydration through a graded ethanol series ...
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bioRxiv - Pathology 2023Quote: ... The aqueous phase was diluted 1:1000 with 25 mM Tris-acetate buffer pH 7.75 and ATP measured using luciferin-luciferase reagent (Invitrogen, #A22066). Background was estimated by omission of the incubation step.
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bioRxiv - Biochemistry 2023Quote: ... both tRNAPHE and total tRNA were digested in RNase/DNase free water made 200 mM with ammonium acetate(Invitrogen, CA.), followed by introduction of 50U RNase T1 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: 20~60μg lysates from culture cells or mouse tissues were separated on 10% Bis-Tris or 3~8% Tris-Acetate Novex NuPAGE gels (Invitrogen) and transferred to nitrocellulose membrane following standard procedures ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL of ion-pairing agent (0.1% o-toluidine blue, Carolina Biological) and 3 µL of ethyl acetate (ACS grade, Fisher Scientific). The mixture was vortexed and centrifuged briefly ...
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bioRxiv - Microbiology 2023Quote: ... then 5 μg total protein in this buffer was separated by SDS-PAGE on a NuPAGE 3-8% Tris-Acetate Midi Gel (Invitrogen). Protein was transferred to a 0.45 μm PVDF Membrane (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was subjected to Western blot on NuPAGE Novex 3–8% Tris-acetate gradient gels (Invitrogen™ # EA0375BOX), transferred to a nitrocellulose membrane and blotted against OPA1 (Anti-Mouse ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled at 95°C for 5 mins and spun at 21 300 rcf for 5 mins prior to loading in NuPAGE Tris-Acetate gels (Invitrogen). Gels were run at 150 V for 1 h and transferred onto 0.2 µm PVDF membranes using the High MW setting on the Trans-Blot Turbo (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... 5µl samples of the PCR2 products were fractionated on a 2% agarose Tris-Acetate-EDTA (TAE) gel using Sybr Safe dye (Invitrogen) alongside a set amount of 100bp DNA ladder (NEB ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were visualized by electrophoresis on 0.5% Tris-acetate-EDTA (TAE) agarose gel at 100 V for 1 h using SYBR Safe DNA Gel Stain (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... were stained with uranyl acetate and Reynolds lead citrate and examined at 100 kV with a PHILIPS EM208S TEM (FEI - ThermoFisher) equipped with the Megaview III SIS camera (Olympus).
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bioRxiv - Microbiology 2024Quote: ... Protein extract was heat-denatured for 10 min at 80°C in NuPAGE LDS Sample Buffer an an equivalent of 2 µg total protein was separated by SDS-PAGE on a NuPAGE 4-12% Tris-Acetate Midi Gel in MOPS buffer (Invitrogen). Protein was transferred to a 0.45 µm PVDF Membrane (Cytiva) ...
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bioRxiv - Neuroscience 2023Quote: ... Purified proteins were stored at −80°C or refolded by dialysis against refolding buffer (20mM sodium acetate and 0.005% NaN3 (pH 4.5)) using SnakeSkin™ Dialysis Tubing (MW cut-off: 3500 Da, Thermo Scientific). Purified proteins were analyzed by SDS-PAGE under reducing conditions.
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bioRxiv - Biochemistry 2023Quote: The protein preparation was stored at −20°C before being buffer exchanged into 200 mM ammonium acetate (pH 6.8) by multiple rounds of concentration and dilution using the PierceTM protein concentrators (Thermo Fisher). The sample was then diluted to 2 µM monomer concentration immediately before the measurements ...
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bioRxiv - Biochemistry 2023Quote: ... buffer was exchanged to 200 mM ammonium acetate (pH 4.5) with a Zeba™ 7 K MWCO desalting column (ThermoFisher). After buffer exchange ...
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bioRxiv - Biochemistry 2023Quote: ... Concentrated virus was applied through a 0.45 μm surfactant free cellulose acetate syringe filter (Nalgene, Fisher Scientific #09-740-106) and immediately used or stored at -80ºC for up to one month ...
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bioRxiv - Cell Biology 2023Quote: ... NeutrAvidin bead acetylation was necessary to stop the Neutravidin being cleaved from the agarose beads during on-bead digestion and performed prior to the day of pulldown by two 30 min incubations of beads with 10 mM Sulfo-NHS acetate (ThermoFisher) on a rotating wheel followed by quenching in 90 mM Tris-HCl pH7.5 ...
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bioRxiv - Microbiology 2023Quote: ... carrying plasmids expressing DB-fused NS5 sequences were co-transformed using a standard lithium/acetate procedure with 10 ng of linearized pPC86 empty vector (Invitrogen) and 3 µL of PCR products generated from cDNA encoding TYK2 ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE western blot was performed by loading 20 μg of each S-fraction and equal volume of the P-fraction onto precast Tris-Acetate SDS-PAGE (Novex, Invitrogen). Western blots were performed as before ...
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bioRxiv - Cell Biology 2024Quote: ... Resuspend in 50-500 µL of 1.11x tagmentation buffer (66 mM Tris-acetate (pH=7.8, BP-152, Thermo Fisher Scientific), 132 mM K-acetate (P5708 ...
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bioRxiv - Neuroscience 2022Quote: ... SDS-PAGE western blot was performed by loading 20 μg of each S-fraction and double the volume of the P-fraction onto pre-cast Tris-Acetate SDS-PAGE (Novex, Invitrogen). Western blots were performed as before ...
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bioRxiv - Molecular Biology 2022Quote: ... quenched in 100 mM Tris-HCl pH 8.0 and analyzed on a NuPAGE™ 3–8 % Tris-Acetate gel (Invitrogen). Fractions containing protein bands corresponding to the expected size of a Pol II–APT complex were dialyzed against 10 mM K-HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was eluted in 50 µl water and precipitated in 0.75 M ammonium-acetate and 10 µg glycogen (#R0551 Thermo Scientific) by adding 125 µl ethanol (100%) ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were size-fractionated on precast 10 cm 4-12% Bis-Tris or Tris-acetate gels (Thermo Fisher Catalog #NP0336BOX), then electroblotted onto nitrocellulose membranes (Thermo Fisher Catalog #88025) ...