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Citations for Thermo Fisher :
5401 - 5450 of 6821 citations for Recombinant Human IL1RL1 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and for human biopsies (see Fig. S1B and C) goat anti-ZO-1 (Thermo Fisher; PA5-19090; 1:200) and anti-laminin primary antibodies were used ...
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bioRxiv - Bioengineering 2023Quote: ... Two different lots (lot 1355434 and 1767629) of human dermal neonatal foreskin fibroblasts (hDFn) were purchased from Thermo Fisher Scientific (cat ...
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bioRxiv - Bioengineering 2022Quote: Human osteosarcoma U2OS cell lines (HTB-96, ATCC, USA) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, GIBCO, USA) containing 10% heat- inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: Enriched neutrophils were resuspended at 1×106 cells/mL in RPMI with 20 ng/mL GM-CSF (R&D or STEMCELL) (for murine neutrophils) or RPMI with 2% FBS (for human neutrophils) with 1μM Sytox Green (Invitrogen) and plated in 96-well plates at 200,000 neutrophils per well ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were grown to log phase in the presence of 1:200 pooled normal human serum (Thermo Fisher Scientific) to opsonize bacteria ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA encoding full-length human CLASP2α was purchased from Dharmacon (Accession: BC140778.1) and subcloned into a pFastBacHT vector (Invitrogen) containing an N-terminal 6xHis-tag ...
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bioRxiv - Cell Biology 2022Quote: ... Endometrial ADGR gene expression in these 21 samples was determined using the high-density human genome (HG) U133 Plus 2.0 Arrays (Affymetrix), containing 42,203 genes and 12,397 expressed sequence tags (ESTs) ...
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bioRxiv - Cancer Biology 2023Quote: Human embryonic kidney 293T (HEK293T) cells were purchased from ATCC and cultured in DMEM media (Thermo Fisher, 11965-092) with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Cancer Biology 2023Quote: ... The human GCB-DLBCL cell line SU-DHL4 (RRID:CVCL_0539) was obtained from DSMZ and grown in RPMI 1640 (ThermoFisher; 11875093) with 10% FBS ...
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bioRxiv - Genomics 2023Quote: ... in condΔEXP168 were maintained in 3% hematocrit in human O+ blood in RPMI 1640 supplemented with 5% Albumax-II and 50µg/ml hypoxanthine (Gibco), 25mM HEPES (Sigma ...
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bioRxiv - Genetics 2022Quote: ... and human CD45+ (hCD45+) bone marrow (BM) cells from transplanted mice using the PureLink Genomic DNA Mini Kit (Invitrogen). DNA was digested using DraI restriction enzyme (NEB ...
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bioRxiv - Immunology 2023Quote: ... human stool extracts and mouse colonic tissue lysates were resolved on Bolt 4-12% Bis-Tris Plus Gels (Invitrogen), transferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Immunology 2023Quote: ... and HEK293T (human embryonic kidney cells, ATCC CRL-3216) cells were maintained in Dulbecco’s modified Eagle medium (DMEM) (Gibco). All media were supplemented with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Immunology 2023Quote: ... the blot was labelled with horse-radish peroxidase (HRP) conjugated goat anti-human IgG antibody (Invitrogen, catalog number 31410) using a 1:3333 dilution in TBS-T ...
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bioRxiv - Immunology 2023Quote: ... the blot was labelled with horse-radish peroxidase (HRP) conjugated goat anti-human IgG antibody (Invitrogen, catalog number 31410) using a 1:3333 dilution in TBS-T ...
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bioRxiv - Developmental Biology 2023Quote: WTC-mTagRFPT-LMNB1 human iPSCs were dissociated into single cells with 5 min incubation in Accutase (Life Technologies, #A1110501) at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Each pool was labeled and hybridized to Affymetrix® Human Gene 2.1 ST according to the manufacturer instructions (Affymetrix). Quality control and normalization of Affymetrix.CEL files using the Robust Multi-array Average (RMA ...
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bioRxiv - Immunology 2023Quote: ... and TNF-α (cat#BMS223-4) were measured in duplicate using human ELISA kits following the manufacturer’s instructions (ThermoFisher) and SpectraMax M3 (Molecular devices).
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bioRxiv - Genomics 2023Quote: ... falciparum clone II313 was maintained in human A+ erythrocytes at 37 °C in RPMI 1640 medium containing AlbumaxII (Invitrogen) supplemented with 2 mM L-glutamine ...
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bioRxiv - Developmental Biology 2023Quote: ... and/or ALK1 (SMARTpool ON-TARGETplus Human ACVRL1 siRNA, Dharmacon L-005302-02-0005) using Lipofectamine 3000 (ThermoFisher L3000015) according to manufacturer directions ...
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bioRxiv - Microbiology 2023Quote: Human embryonic kidney 293T cells (HEK293T) and Vero E6 cells were maintained in Dulbecco modified Eagle’s medium (DMEM; Gibco) with 8% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... after which they were washed with 1% human albumin in PBS and stained with propidium iodide (Molecular Probes #P3566) for assessing viability ...
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bioRxiv - Cell Biology 2023Quote: ... The digested tissue was mixed with an equal volume of 1% human albumin (CSL Behring) in DPBS −/− (Gibco #14190094) to stop the lysis ...
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bioRxiv - Neuroscience 2023Quote: ... engraftment was measured weekly by quantifying human CD45+ cells in each mouse’s peripheral blood through fluorescence-activated cell sorting (FACS) on Attune flow cytometer (ThermoFisher). On average ...
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bioRxiv - Microbiology 2023Quote: ... Cat#40590-D001) and anti-human AF488 (1:1000 with final concentration of 2μg/ml; ThermoFisher Cat#A-11013) for secondary conjugation ...
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bioRxiv - Immunology 2023Quote: Human NK cells were seeded at a density of 2×106 cells/mL in a 24-well plate (NUNC) in the presence of IL-15 (10 ng/mL ...
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bioRxiv - Bioengineering 2023Quote: Human breast cancer cell lines MDA-MB-231 (ATCC) and MCF-7 (ATCC) were grown in DMEM/F12 (Gibco) with 15 mM HEPES supplemented with 10% v/v fetal bovine serum (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... Transferrin (loading control) was detected with mouse anti-human transferrin receptor antibody (1:500, Cat# 136800, Invitrogen, Waltham, MA). Protein samples (50 µg ...
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bioRxiv - Neuroscience 2023Quote: ... Amyloid Aβ40 and Aβ42 in the hippocampus and striatum and D2R in the striatum were quantified by ELISA with the following kits: Aβ40 human ELISA kit (Thermofisher); Aβ42 human ultrasensitive ELISA Kit (Thermofisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... with the human dystrophin 3′ UTR or mutant 3′ UTR and with 50 nM miR-146a mimic (Life Technologies) with Lipofectamine 2000 according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Genetics 2024Quote: ... 3% human serum (heat-inactivated from Sigma-Aldrich, St. Louis, MO, USA or Thermo Fisher Scientific, Waltham, MA, USA), 2% human plasma (isolated from umbilical cord blood provided by Stanford Binns Cord Blood Program) ...
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bioRxiv - Cell Biology 2023Quote: ... and separately two Silencer Select siRNAs targeting human Myosin ID (4392420; ID s9203 and s9204; Invitrogen, Thermo Fisher Scientific) at a final concentration of 0.01 uM using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: MS raw data were searched against the UniProtKB human sequence data base (92,954 entries) and TMT 10plex quantitation performed using Proteome Discoverer software (v 2.3; Thermo Scientific). Search parameters were set to include carbamidomethyl (C ...
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bioRxiv - Microbiology 2024Quote: Human cervix adenocarcinoma cells (HeLa 229, ATCC CCL-2.1TM) were cultured in RPMI-1640 medium (Thermo Fisher Scientific, 72400054) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then treated with human serum containing phospholipase D (Australian Red Cross Lifeblood) or 0.25% (w/v) Albumax II (Gibco) and incubated for 3 hours at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and separately two Silencer Select siRNAs targeting human Myosin ID (4392420; ID s9203 and s9204; Invitrogen, Thermo Fisher Scientific) at a final concentration of 0.01 uM using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: HeLa human cervical cancer cells and HepG2 human hepatocellular carcinoma cells were cultured in high-glucose Dulbecco’s modified Eagle medium (DMEM, Gibco) supplemented with 4.5 g/L glucose ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Tg tachyzoites were maintained in vitro by serial passage on confluent monolayer of human foreskin fibroblasts (HFF, ATCC SCRC-1041) in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 1% (vol/vol ...
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bioRxiv - Molecular Biology 2023Quote: The human RPE cell line ARPE-19 was cultured in DMEM/F-12 (Cat #C11330500BT, Gibco, Waltham, MA, USA) media with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... DRG expression levels were determined from total RNA extracts measured using Affymetrix’ Human Transcriptome Array 2.0 (Affymetrix, Santa Clara), scanned with Affymetrix’ GeneChip 3000 G7 Instrument System ...
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bioRxiv - Neuroscience 2023Quote: ... Aβ concentrations in three separate fractions of brain homogenates were determined by Invitrogen™ Amyloid β 42 and 40 Human ELISA kits (Cat.-No.: KHB3441 and KHB3481, respectively; both from Thermo Fisher Scientific). Results were normalized on the basis of the sample’s protein concentration.
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bioRxiv - Microbiology 2023Quote: The adult human dermal fibroblast cell line (HDFα, FH1092, FuHeng Biology) was cultured in RPMI 1640 medium (11875093, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... were propagated in 5% of human O+ erythrocytes and 10 mL of RPMI-1640 medium containing 0.5% Albumax II (Invitrogen), 2 mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: All parasite lines were maintained in human foreskin fibroblasts (HFFs; ATCC) in Minimal Essential Media-α (MEM-α; Gibco) supplemented with 7% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: The gene sequences of human MCHR1(residues 70-405) and MCHR2(residues 1-331, with L2566.40Y mutation) was subcloned into pFastBac vector (Invitrogen) with an N-terminal haemagglutinin signal peptide (HA ...
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bioRxiv - Biochemistry 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a human IL-8 ELISA kit (Invitrogen).
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney (HEK293) and A549 cell lines were maintained in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Lithuania) containing 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA coding for the protein sequences was synthesized using codon-optimization for human host cells (Thermo Fisher Scientific). If the codon optimization for the lysine-stretches resulted in six or more consecutive adenines in the DNA ...
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bioRxiv - Microbiology 2023Quote: Mouse fecal pellet and human stool sample data analysis was performed using FreeStyle software (version 1.8 SP2, Thermo Scientific). Metabolite identification was established by matching accurate mass ...