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Citations for Thermo Fisher :
5651 - 5700 of 6821 citations for Recombinant Human IL1RL1 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... T cells were stimulated for 2 days at a starting density of approximately 1 million cells per mL of media with anti-human CD3/CD28 Dynabeads (ThermoFisher), at a bead to cell ratio of 1:1 ...
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bioRxiv - Microbiology 2019Quote: ... and HEK293T (embryonic human kidney epithelial, ATCC®CRL-3216) cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2019Quote: ... The immortalized human upper airway epithelial cell line A549 (ATCC# CCL-185) was maintained in minimal essential medium (MEM; Gibco) supplemented with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2019Quote: ... were incubated for 15 minutes at 37°C in the presence of Alexa-fluor 555 labelled human transferrin (Thermo Fisher) (25 μg/ml ...
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bioRxiv - Microbiology 2019Quote: ... the membrane was probed with HIV-Ig as the primary antibody and corresponding secondary antibody conjugated to horseradish peroxidase (goat anti-human IgG, Invitrogen). Gag-specific bands were detected after incubation with SuperSignal West Pico chemiluminescent substrate (Pierce ...
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bioRxiv - Cancer Biology 2019Quote: ... Precursors of respective human microRNAs (miR-27a, −122, −128, −193 and −217) and negative controls were purchased from Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Samples were analyzed on the GeneChip Human Gene 2.0 ST Array chip hybridized on the GeneChip Scanner 3000 7G System (Applied Biosystems) according to the manufacturer’s recommended instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Medium from each condition was removed and E-cadherin concentrations were quantified by using Human E-Cadherin Quantikine ELISA kit (Invitrogen). The Human E-Cadherin EIA kit protocol was followed as described ...
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bioRxiv - Immunology 2019Quote: ... Concentrations of 30 cytokines were measured using the 30-plex magnetic human Luminex cytokine kit (all using lot ID 1805187A, ThermoFisher). Samples were measured on a LX200 (Luminex ...
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bioRxiv - Bioengineering 2019Quote: ... rinsed and re-suspended in 100 μL of 25 μg mL−1 mouse anti-human CD3-AF700 (56-0037-42, ThermoFisher), CD4-PE-Cy7 (25-0048-42 ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... falciparum parasite culture was maintained in 5% human red blood cells purified from whole blood (Research Blood Components) with complete RPMI medium (Gibco) supplemented with hypoxanthine (Sigma-Aldrich) ...
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bioRxiv - Genomics 2019Quote: Hand-picked human pancreatic islets were dissociated to single cells using enzyme-free Hanks’-based Cell Dissociation Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... and treated without (vehicle) or with Gibco™ Dynabeads™ Human T-Activator CD3/CD28 (Cat. No. 111.61D, ThermoFisher Scientific) in the presence of recombinant IL2 and BD GolgiPlug (BD Biosciences ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 × 106 human cells in 35 mm dish per sample were washed with 1 × PBS and lysed with 600 μl DNAzol (Invitrogen), followed by precipitation with 300 μl 200 proof ethanol ...
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bioRxiv - Cell Biology 2021Quote: ... WT human PRRT2 (gene ID: 112476) was synthesized as N-terminal HA tag fusion protein (Genewiz) in pcDNA3 vector (Thermofisher) and single point mutants were generated on this matrix by standard molecular biology techniques.
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bioRxiv - Neuroscience 2021Quote: ... tau proteins were measured with Tau-5 antibodies using ELISA according to manufacturer’s instructions (The Human Tau Solid-phase sandwich ELISA, #KHB0041, Thermo Scientific).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... as well as human hepatoma Huh7.5 cells28 were maintained at 37 °C with 5% CO2 in Dulbecco’s Modified Medium (DMEM) (Invitrogen, Paisley, UK) containing 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Parasites were maintained by continuous passage in human foreskin fibroblasts (HFFs) in Dulbecco’s Modified Eagle’s Media (DMEM) (ThermoFisher, Carlsbad CA) containing 1% (v/v ...
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bioRxiv - Genomics 2020Quote: Female human bone osteosarcoma U-2 OS cells (ATCC HTB-96) were routinely cultured in McCoy’s 5A (Modified) medium (ThermoFisher 16600) with 10% FBS (Gemini Bio ...
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bioRxiv - Molecular Biology 2021Quote: ... Human globin mRNA was depleted from all samples except from samples #1 and #2 using the GLOBINclear kit (ThermoFisher Scientific). The quality of the RNA was assessed using the Agilent 6000 Pico kit with the Bioanalyzer 2100 (Agilent ...
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bioRxiv - Cancer Biology 2020Quote: ... isolation media was changed to human liver expansion media (EM; Ad+++ supplemented of 1X B27 supplement without retinoic acid (Gibco), 1X N2 supplement (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells and human mesenchymal stem cells were grown in DMEM culture medium supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 mg/ml streptomycin and 100 units/ml penicillin (Gibco ...
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bioRxiv - Immunology 2020Quote: ... and resuspended in complete medium in the absence of human serum or Pf-iRBCs schizonts were isolated in RPMI 1640 medium supplemented with 0.25% Albumax (GIBCO, Invitrogen) and 7.4% Sodium Bicarbonate (GIBCO ...
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bioRxiv - Immunology 2020Quote: ... Mass spectrometry derived data was searched against Human RefSeq 81 protein database in Proteome Discoverer 2.1 (Thermo Scientific, Bremen, Germany) using SEQUEST and Mascot (version 2.5.1 ...
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bioRxiv - Molecular Biology 2021Quote: Human proximal renal tubular epithelial cells (HK2) were purchased from ATCC (ATCC®CRL-2190) and cultured in DMEM (Gibco) supplemented with 10% FBS (Moregate) ...
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 (Apath) cells were maintained in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen, 10-013-CV); African green monkey kidney Vero (ATCC ...
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bioRxiv - Microbiology 2021Quote: ... and the N-terminal peptidase domain of human or mouse ACE2 (residues Ser19–Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as described previously44 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were then washed and incubated with PE-conjugated goat anti-human Fc antibodies (Thermo Fisher 12-4998-82) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Freshly isolated HTII-280+ and HTII-280− cells derived from CD31−CD45−EpCAM+ cells of human distal lungs were resuspended in Advanced DMEM/F12 (Thermofisher) base medium supplemented with 10 mM Hepes (Gibco) ...
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bioRxiv - Developmental Biology 2020Quote: ... mosquitoes were offered a sterile bloodmeal consisting of 50 % human red blood cells (Etablissement Français du Sang de Guadeloupe-Guyane) and 50 % foetal bovine serum (Thermo Fisher). The bloodmeal was provided to mosquitoes in an autoclaved Hemotek reservoir (Hemotek ...
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bioRxiv - Biophysics 2021Quote: The immortalized hTERT RPE1 cells (Human Retinal Pigmented Epithelium) were cultivated in DMEM F12 without Phenol Red (Gibco, Life Technologies) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biophysics 2021Quote: The immortalized hTERT RPE1 cells (Human Retinal Pigmented Epithelium) were cultivated in DMEM F12 without Phenol Red (Gibco, Life Technologies) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: The template RNA for cDNA synthesis was isolated from human HEK293 cell culture with TRIzol reagent (Thermo Fisher Scientific, USA), or FFPE samples ...
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bioRxiv - Developmental Biology 2021Quote: ... embedded in GFR Matrigel, and cultured in human organoid expansion medium (Boj et al., 2015) (AdDMEM/F12 medium supplemented with HEPES (1x, Invitrogen), Glutamax (1x ...
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bioRxiv - Immunology 2021Quote: - IFNγ and IL-10 levels were measured in the supernatant of the WBA using IFN gamma and IL-10 Human ELISA Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Monocytes were then differentiated into macrophages by 7-9 days of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Immunology 2020Quote: ... Digested sera was probed with Rabbit anti-Human IgG-HRP H+L Secondary Antibody (ThermoFisher Scientific Catalog #A18903, 1:10,000), Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423 ...
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bioRxiv - Immunology 2020Quote: ... African Green monkey kidney epithelial cells) and Huh-7 cells (human hepatocellular carcinoma cells) were cultured in Dulbecco’s minimal essential medium (DMEM; Life Technologies), supplemented with 10 % fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2019Quote: IMR-90 primary human lung fibroblasts (ATCC #CCL-186) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco #12430-054) supplemented with penicillin and streptomycin (5000 U/mL and 5000 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... Macrophages were obtained from the EpCAM− cells after 2 hours of adherence to the tissue culture dish and were cultured with 50 ng/ml human macrophage colony-stimulating factor (M-CSF, #CYT-308, ProSpec) in RPMI-1640 (Gibco) containing 20% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2021Quote: A total of 10 technical replicates of human neuroblastoma SH-SY5Y cells were cultured in neurobasal media (Gibco 21103-049) supplemented with B-27 Plus (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... In order to generate the construct for inducible ZEB2 expression from the AAVS1 site the coding sequence of the human ZEB2 transcript variant 1 (NM_014795.3) was purchased as an ultimate ORF clone in the pENTRY221 (Thermo Scientific, #IOH53645). The ZEB2 ORF was amplified with primers ZEB2_IndOE_F & _R and tagged with GFP-Flag by Gibson assembly into the pT2-CAG-MCS-2A-Puro plasmid linearized by restriction digestion with EcoRI (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... CPA and CPD cells were cultured in Keratinocyte-SFM supplemented with 2.5 μg prequalified human Epidermal Growth Factor 1-53 (EGF) (Life technologies), 25 mg Bovine Pituitary Extract (BPE ...
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bioRxiv - Cell Biology 2021Quote: ... immortalized human lung fibroblast cells and HCT116 colorectal cancer cells were cultured in a 1:1 mixture of DMEM (Gibco) and Ham’s F10 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... kind gift from Peter Bredenbeek, LUMC, NL) and HuH7 (human hepatoma, JCRB0403) cells were maintained in minimal essential medium (Gibco) supplemented with 10% fetal bovine serum (Integro) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured in BBB induction medium: human endothelial cell serum-free medium (HESFM) (Cat. no. 11111044, Thermo Fisher Scientific) supplemented with 1% human platelet poor derived serum (Cat ...