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Citations for Thermo Fisher :
5351 - 5400 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Secondary AlexaFluor-conjugated antibodies (Life Technologies; 1:500 in 5% BSA in PBS) with Hoechst 33342 dye were incubated for 1 h at room temperature in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... and 5 µM EGTA) with 1 µM of Calcium Green™-5N (Invitrogen). 30 µM of CaCl2 was injected into wells at a specific time ...
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bioRxiv - Neuroscience 2023Quote: ... Supplemental Table 1) and the QuantStudio 5 Real-Time PCR System (Applied Biosystems). Relative gene expression (RQ ...
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bioRxiv - Bioengineering 2023Quote: ... with supplemented RPMI (95% RPMI, 5% FBS, 1% penicillin/streptomycin: Life Technologies, NY) at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... 10 mM EGTA) and coated with anti-biotin (5% in 1× SAB; Invitrogen), and the nonspecific surface was blocked with Pluronic F127 (1% in 1× MRB80 ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x103 MCF7 were plated to 1 ml DMEM/F12 (Invitrogen, Catalog # C11765500BT) medium containing B27 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were counterstained for 5 min with Hoechst (1:10000 in PBS; Invitrogen), and after washing ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked in 5% BSA in TBS with 1% Tween (TBST, ThermoFisher) and incubated with primary (overnight ...
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bioRxiv - Microbiology 2024Quote: ... rabbit polyclonal anti-nsp3 (Thermo Fisher, PA5-116947, 1:134, 5 µg/mL), sheep polyclonal anti-GFP (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... 1 × 106 HEK293T cells were plated in a 6-well dish with 1.5 mL of DMEM growth media (Gibco) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... according to the manufacturer’s instructions and diluted 1:6 with DNAse/RNAse-free water (Thermo Fisher, Waltham, MA, USA). qRT-PCR was performed with SYBR-Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... embryos at the 3-6 somite stage were embedded oriented laterally in 1% low-melt agarose (Invitrogen, Carlsbad, CA) and imaged under bright field (to determine yolk elongation by taking major and minor axis measurements ...
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bioRxiv - Microbiology 2020Quote: The lipophilic fluorescent membrane probe 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH) (Themo Fisher Scientific) was used to assess EV associated lipids ...
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bioRxiv - Bioengineering 2021Quote: ... was dissolved under stirring in 15 mL of an alginate solution (10 mg/mL-1) formed in 0.1 M MES buffer at pH 6 (ThermoFisher). Sulfo-NHS (41 mg ...
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bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
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bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were seeded at low and high density in 6-or 12-well plates coated with 0,2% type 1 Collagen (Gibco) and grown in PM for 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... NALM-6 cells were cultured in RPMI media supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco). MCF7 cells were cultured in DMEM media supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: ... 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 6% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (Gibco, 15240062). T cell lines were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Immunology 2020Quote: 250 μL K92 peptide at 1 mg/mL was reduced with 6 μL of 0.5 M Dithiothreitol (Thermo Fisher) for 40 minutes at 37 °C and alkylated with addition of 18 μL of 2-Iodoacetamide (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... 60 °C for 1 min) X 49 cycles on the QuantStudio 6 flex Real-time PCR system (Applied Biosystems), using the TaqMan RNA-to-CT 1-step kit (Applied Biosystems).
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bioRxiv - Immunology 2023Quote: ... and rested for ∼6 hours in R15 (RPMI 1640 medium supplemented with 15% FBS, 1% antibiotic-antimycotic [Thermo Fisher Scientific] ...
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bioRxiv - Cell Biology 2023Quote: ... 8 days after transfection antibiotic selection was performed using 6 µg mL-1 blasticidin S (Thermo Fisher Scientific, A1113903) to identify cells which had undergone productive editing ...
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bioRxiv - Physiology 2024Quote: ... Cells were grown overnight in glass slides and loaded with 10-6 mol.L-1 4,5-diaminofluorescein-diacetate (DAF-2DA; Molecular Probes) for 30 minutes ...
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bioRxiv - Pathology 2024Quote: ... IRB number: VGHUST105-G7-6-1) were cultivated in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Neuroscience 2024Quote: ... or 5-TAMRA (5-Carboxytetramethylrhodamine) (Thermo Scientific) in 0.1M Na2CO3 (pH 9.0 ...
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bioRxiv - Cancer Biology 2019Quote: ... 300,000/6-well) and IDC (5,000/96-well; 100,000/6-well) cells were seeded in regular (Thermo Fisher Scientific) or ULA (Corning Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were forward transfected in 6-well dishes by using 6 μl of FuGENE® HD (PRE2311, Fisher Scientific) with 3 μg of sgRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... for the first 24 hours and for further 4 to 6 days in Essential 6 media (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Cell Biology 2020Quote: Cells in the 6-well plates were transfected with 2μg plasmid of AMPKα or OGG1 via 6 μl Lipofectamine 2000 (Invitrogen) for 6 hours following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The Cas9-sgRNA-containing plasmid and donor vector were co-transfected at a molar ratio of 1:6 into XX mESCs cells on a 6-well plate using Lipofectamine 2000 according to the manufacturer’s protocol (ThermoFisher). Transfected cells were passaged at different densities into three Petri dishes with feeders ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1X MEM non-essential amino acids (NEAA) (Gibco), 1 mM sodium pyruvate (Merck) ...
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bioRxiv - Biophysics 2021Quote: ... 1x MEM non-essential amino acids solution (ThermoFisher), and 2 mM L-glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... 1x of non-essential amino acids (Life Technologies) and 1x penicillin/streptomycin (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... Bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific) was performed to determine protein concentration ...
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bioRxiv - Cell Biology 2020Quote: ... and 10mM non-essential amino acids (all Gibco) at 4°C overnight (RPMI medium hereafter) ...
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bioRxiv - Cell Biology 2020Quote: ... Fatty Acid-Free Powder (Thermo Fisher Scientific – BP9704100), Fetal bovine serum (Wisent – 095-150) ...
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bioRxiv - Developmental Biology 2020Quote: ... 1x non-essential amino acid (NEAA; ThermoFisher, 11140050), 2 μg/ml Heparin (ThermoFisher ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1mM non-essential amino acids (Invitrogen cat # 11140050), 1mM pyruvate (Invitrogen cat # 11360070) ...
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bioRxiv - Microbiology 2020Quote: ... 25% acetic acid (Fisher Scientific, Pittsburgh, PA, USA), and 15% water ...
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bioRxiv - Molecular Biology 2021Quote: ... or bicinchoninic acid (BCA) assay (Thermo Fisher Scientific). 20 µg of protein lysates were separated by size ...
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bioRxiv - Developmental Biology 2021Quote: ... 1x nonessential amino acids (Invitrogen cat. 11140-036), 1 mM sodium pyruvate (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... non-essential amino acids (Invitrogen, Carlsbad, CA, 1003), 100 μM b-mercaptoethanol (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... 1x nonessential amino acids (100x, Gibco, Lot # 2027433), 1x L-Glutamate (100x ...
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bioRxiv - Developmental Biology 2020Quote: ... 1Xnon-essential amino acids (Thermo Fisher Scientific, USA), 2 mM L-glutamine (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... non-essential amino acids (NEAA) (Thermo Fisher Scientific), and penicillin and streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... and 1x non-essential amino acids (NEAAs, Gibco). The human monocytic cell line MonoMac6 (Ziegler-Heitbrock et al ...
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bioRxiv - Molecular Biology 2021Quote: ... A bicinchoninic acid (BCA) assay kit (Thermo Fisher) is used to standardize the samples to the same protein level ...