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Citations for Thermo Fisher :
5301 - 5350 of 10000+ citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: Aiptasia or Nematostella polyps were relaxed for 15 min in 0.1M MgCl2 in sea water or Nematostella medium and transferred to sea water or Nematostella medium containing 300 µM EdU (5-ethynyl-2′-deoxyuridine, Invitrogen) 2%DMSO 0.1M MgCl2 ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Cancer Biology 2023Quote: The sonicated samples (approximately 5 µg chromatin/sample) were cleared for 2 hours using non-coupled magnetic beads (Protein G Dynabeads, Invitrogen), then further mixed with 5 µL antibody (anti-H3K27me3 ...
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bioRxiv - Developmental Biology 2023Quote: Timed pregnant females were injected intraperitoneally with 13 μg per g body weight of 5-ethynyl-2’-deoxyuridine (Edu; Invitrogen) and sacrificed after 1 hour or 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and an Acclaim PepMap 100 C18 HPLC nanoViper Trap Column (100Å, 100 μm X 2 cm, 5 μm; ThermoFisher, USA). The tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Microbiology 2023Quote: ... approximately 5×105 Huh-7 cells were transfected with 2 µg of purified BAC DNA using Lipofectamine 3000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Genomics 2023Quote: ... For the construction of the RNH2A KO clones, RNASEH2A (Chr19, exon 2) gRNA (5’-TAACAGATGGCGTAGACCAT-3’) was cloned into GeneArtTM CRISPR Nuclease Vector with OFP reporter (Invitrogen) following manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... was washed with wash buffer II (150mM NaCl, 50mM Tris pH 7.5, 5% glycerol) on the DynaMag-2 magnet (ThermoFisher Scientific) and added to each lysate and rotated for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... TEV (2): 50 µg H*-Cdc42:CTIS was mixed with 5 or 50 U of AcTEVTM protease (10 U/µL) (Invitrogen) in SEC buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... cells at exponential growth phase were incubated with 200 μM of the thymidine analog EdU (5-ethynyl-2′-deoxyuridine - Invitrogen) at 26 °C for 10.2 h (estimated doubling time of L ...
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bioRxiv - Microbiology 2023Quote: ... 4×105 spores and 200µl of a bacterial suspension were spread on SM/5 agar plates ( 2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Molecular Biology 2023Quote: Protein (2 µg total lysate, 5 µg soluble lysate) was loaded onto 4-12% Bis-Tris NuPAGE minigels (Invitrogen, NP0322BOX). 10 µL of precision plus protein kaleidoscope prestained standard (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... and an Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100 Å; Thermo Scientific) were used to separate tryptic peptides by increasing concentrations of 80% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Intracellular NO production was assessed during reoxygenation by live-cell staining with the NO-sensitive fluorescent dye 4-Amino-5-Methylamino-2’,7’-Difluorofluorescein (DAF-FM) diacetate (Invitrogen). iPSC-ECs were stained with 1 μM DAF-FM diacetate for 10 minutes at 37°C ...
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bioRxiv - Genetics 2024Quote: ... NaCl and ZnCl2 for 2 hours with agitation at 800 rpm for 30s every 5 min at 37°C in thermomixer (Thermofisher), followed by addition of NH4CO3 (Sigma ...
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bioRxiv - Immunology 2024Quote: ... and cultured for 5 days in AIMV medium supplemented with 10% fetal calf serum (FCS) and 100 U/ml of rhIL-2 (Gibco). 8×106 CTLs were transfected with 6 µg of plasmid DNA of each construct with P3 Primary Cell 4D-Nucleofector X Kit (Lonza) ...
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bioRxiv - Biochemistry 2024Quote: ... 25 μl/ml BioLock biotin blocker (IBA, 2-0205-050) and 5 μl Pierce Universal Nuclease (Thermo Fisher Scientific, 88702), and kept on ice ...
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bioRxiv - Cell Biology 2024Quote: ... We removed the supernatant and gently resuspended cells with 2 ml 1X PBS containing 5 μl SUPERaseIn RNase Inhibitor (Invitrogen) and 2.5 μl RiboLock RNase Inhibitor (Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... The cultures were maintained at 37°C in a humidified atmosphere of 5% CO2 in Neurobasal medium supplemented with 2% B27 (Invitrogen), 1% Glutamax (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 min at RT and blocked in PBS with 0.1% Triton X-100 and 2% NGS (Fisher Scientific, 11540526) for 30 minutes at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... and CAF-1 p60 (5′-AAUCUUGCUCGUCAUACCA-3′) were transfected using RNAi MAX (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... 5 µg/mL Blasticidin S HCl and 1 µg/mL Puromycin Dihydrochloride (Gibco) were used to select for stable transformants.
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bioRxiv - Developmental Biology 2021Quote: ... and quenched 1:5 in DMEM supplemented with 10% FBS (Life Technologies, 10270106). Cells were monthly tested for mycoplasma contamination by PCR.
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bioRxiv - Microbiology 2020Quote: ... grown in DMEM with 5% fetal bovine serum and 1% antibiotic/antimytotic (Gibco). Calu3 2B4 cells were grown in DMEM with 10% defined fetal bovine serum ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 5 minute incubation in DAPI (1:5,000; ThermoFisher Scientific, Waltham, MA). Slides were then washed ...
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bioRxiv - Cell Biology 2019Quote: ... containing 5% heat-inactivated fetal bovine serum (FBS) and 1% penicillin/streptomycin (Invitrogen) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: Cells were stained with Nile Red (5◻μg.mL−1 final concentration; Invitrogen) according to Cakmak (2012 ...
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bioRxiv - Microbiology 2022Quote: ... respectively) and 5 μl of 4X TaqMan Fast Virus 1-step mastermix (ThermoFisher). The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... stained for nuclei (DAPI – ThermoFisher Scientific 62248 – 1:2000 for 5 min RT) and mounted with Fluoromount G (ThermoFisher 00-4958-02) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 × 106 cells were stained with 5 µM MitoSOX (Molecular Probes, Thermo Fisher) in PBS at 37°C for 40 min ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 × 106 cells were stained with 5 µM MitoSOX (Molecular Probes, Thermo Fisher) in PBS at 37°C for 40 min ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies: Claudin-5 (mouse, clone 4C3C2, ThermoFisher cat# 35-2500, 1:100), Iba1 (goat polyclonal ...
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bioRxiv - Systems Biology 2019Quote: ... 1: 5 000 000 ERCC RNA Spike-In Mix I (Thermo Fisher Scientific) in RNase-free water ...
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bioRxiv - Microbiology 2019Quote: ... supplemented by 5% iron-supplemented calf serum (HyClone) and 1% penicillin-streptomycin (Invitrogen). Twelve or 89 days post-infection ...
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bioRxiv - Cell Biology 2020Quote: ... the wells were incubated for 5 min with Tetraspeck Beads (Thermofisher, 1:100) and subsequently washed with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was stained for 5 min with 1 μg/ml Hoechst 33342 (Invitrogen) in PBS at room temperature and cells were mounted in Mowiol 4-88 (Calbiochem ...
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bioRxiv - Microbiology 2021Quote: ... Medium was supplemented with chloramphenicol (CAM, 12. 5 μg mL−1, ThermoFisher Scientific) and/or kanamycin (KAN ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% (v/v) Triton X100 and 5 U/ml Turbo DNase (Invitrogen, Cat:AM2238) (Ingolia et al. ...
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bioRxiv - Systems Biology 2021Quote: ... 5% Fetal Bovine Serum (Biowest, Belgium) and 1% antibiotic/antimycotic (Gibco, ThermoFisher Scientific). Chondrogenic differentiation was induced by plating the cells at 6,400 cell/cm² in proliferation medium for 24h ...
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bioRxiv - Systems Biology 2021Quote: ... 5% Fetal Bovine Serum (Biowest, Belgium) and 1% antibiotic/antimycotic (Gibco, ThermoFisher Scientific). Chondrogenic differentiation was induced by plating the cells at 6,400 cell/cm² in proliferation medium for 24h ...
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bioRxiv - Microbiology 2020Quote: ... grown in DMEM with 5% fetal bovine serum and 1% antibiotic/antimytotic (Gibco). Calu3 2B4 cells were grown in DMEM with 10% defined fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... grown in DMEM with 5% fetal bovine serum and 1% antibiotic/antimycotic (Gibco). Standard plaque assays were used for SARS-CoV and SARS-CoV-2 32,33 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with 5 µg/ml FMTM 1-43FX (Cat. #F35355, Invitrogen) for 10 min at 37°C and fixed with ice-cold 4% paraformaldehyde solution as per manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% β-mercaptoethanol and 5 ng/ml bFGF or in StemFlex media (Gibco) on Matrigel-coated dishes (Corning ...
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bioRxiv - Developmental Biology 2022Quote: ... Upon coating with Corning GFR Matrigel (1:5 dilution; Thermo Fisher #CB-40230), salivary glands were laid out avoiding drying and overlaid with organ culture medium containing DMEM/F-12 supplemented with 150 μg/mL vitamin C (MilliporeSigma ...
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bioRxiv - Neuroscience 2023Quote: ... Cy®5 goat anti-mouse IgG (H+L) (Invitrogen, A10524, 1:500)) in TBS ...
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bioRxiv - Neuroscience 2024Quote: ... A 10-µl drop of 5 mM OGB-1 (hexapotassium salt; Life Technologies) in ACSF was suspended from the upper electrode and lowered onto the retina ...
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bioRxiv - Immunology 2024Quote: ... H2-Ld-PE (1:40; clone 30-5-7S; Invitrogen Cat. No. MA518007), CD45-PerCP-Cy5.5 (1:250 ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x103 MCF7 were plated to 1 ml DMEM/F12 (Invitrogen, Catalog # C11765500BT) medium containing B27 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Supplemental Table 1) and the QuantStudio 5 Real-Time PCR System (Applied Biosystems). Relative gene expression (RQ ...