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Citations for Thermo Fisher :
5301 - 5350 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... spun at 200 × g for 10 min and resuspended in macrophage medium (DMEM [Invitrogen] plus 10% FCS and 2 mM L-glutamine) in order to have 2.5 × 106 cells per well ...
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bioRxiv - Microbiology 2021Quote: ... Cells were seeded at a density of 2.7×104 cells/cm2 and cultured in William’s Medium E (Biochrom, Berlin, Germany) containing 10% (v/v) FCS (Life Technologies, Darmstadt, Germany), 5 mg/ml insulin (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: CIL in single cells encountering cadherin coated beads used cdh3-coated beads prepared by binding purified cdh3-fc (gifted from Dr. Douglas W. DeSimone and Dr. Barry. M. Gumbiner) to Protein A/G coated beads (53132, Thermo Fisher Scientific) (Chappuis-Flament et al. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were cultured in Dulbecco’s Modified Eagle Medium (DMEM, High Glucose, GlutaMAX™, Pyruvate) supplemented with antibiotics and 10% FCS (Gibco, Life technologies) and maintained in T75 Flasks at 1×106 cells per flask ...
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bioRxiv - Cell Biology 2020Quote: ... NIH3T3 and RPE-1 were maintained in DMEM (HPSTA – high glucose, stable glutamine and sodium pyruvate; Capricorn Scientific) supplemented with 10% fetal calf serum (FCS; Thermo Fisher Scientific) under standard conditions at 37°C in a 5% CO2 environment ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed with PBS and blocked with R10 media (RPMI-1640 with 10% FCS, 1% Pen/Strep, 2mM L-Glutamine (all from Thermo Fisher Scientific)) for 30 minutes at RT before the addition of freshly isolated PBMCs ...
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bioRxiv - Microbiology 2021Quote: ... OD595 was measured every 5 min at 37°C from cultures that underwent continuous shaking in a Multiskan™ FC Microplate Photometer (Thermo Scientific). Data were collected from at least two independent experiments with at least two replicates for each individual experimental condition.
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bioRxiv - Immunology 2020Quote: ... Staining was performed on ice for 25 minutes in PBS with 2 % FCS using the following antibodies: 7- AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Immunology 2020Quote: ... at a concentration to give 100 to 250 foci per well in the virus only control wells was mixed 50:50 in 1% FCS MEM with 1 x antibiotic/antimycotic (Gibco, 15240-062) with serum doubling dilutions from 1:20 to 1:640 (or higher dependent on antibody levels ...
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bioRxiv - Microbiology 2021Quote: ... Two photometric readings were conducted for each plate with a 540 nm filter on a Multiskan FC Microplate Photometer (Thermo Scientific 51119000). The protocol is a slightly modified version of an Enzymatic Assay of α-Amylase (https://www.sigmaaldrich.com/NL/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-a-amylase ...
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bioRxiv - Immunology 2022Quote: ... 100 µl of 0.2 mM sulfuric acid was added to halt the enzymatic reactions and the optical densities (OD) of the contents in the wells were read at 450 nm using a Multiskan FC plate reader (Thermo Scientific, USA).
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bioRxiv - Cell Biology 2022Quote: ... the absorbance of each well at 450 nm was measured with a microplate reader (Multiskan FC, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... 10% FCS (BioConcept, Cat# 2-01F16-I) and 100 units/ml of penicillin and 100 µg/ml of streptomycin (Invitrogen, Cat# 15140122). Rat primary fibroblasts (REFs ...
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bioRxiv - Microbiology 2022Quote: ... 4a3A cells were maintained at 27°C without CO2 in Insect-XPRESS™ Protein-free Insect Cell Medium complemented with heat-inactivated Fetal Calf Serum (FCS) (#A3840002 ThermoFisher Scientific). C6/36 cell line (ATCC ...
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bioRxiv - Molecular Biology 2024Quote: ... were isolated from mice tibias and femurs as described previously29 and cultured for 7 days in Iscove’s Modified Dulbecco’s Medium supplemented with 10% FCS (Thermo Scientific, Rockford, IL, USA), penicillin-streptomycin (100 U/mL and 100 μg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... cells were kept in MEM Alpha Medium supplemented with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin (Invitrogen GmbH, Darmstadt, Germany) in a humidified atmosphere at 5% CO2 and 37°C ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in complete medium (RPMI 1640 supplemented with 10% fetal calf serum, FCS, 100 U/mL penicillin and 100 μg/mL streptomycin; Sigma-Aldrich and Invitrogen respectively). After the isolation ...
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bioRxiv - Physiology 2024Quote: NIH-3T3 fibroblasts (3T3-FB, 1.0×104 cells) were cultured in 10 % FCS/DMEM (Gibco, Life Technologies, Life Technologies, Carlsbad, CA, US). 3T3-FB were incubated with MNP (E-SO-2-MNP ...
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bioRxiv - Physiology 2024Quote: NIH-3T3 fibroblasts (3T3-FB, 1.0×104 cells) were cultured in 10 % FCS/DMEM (Gibco, Life Technologies, Life Technologies, Carlsbad, CA, US). 3T3-FB were incubated with MNP (E-SO-2-MNP ...
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bioRxiv - Microbiology 2024Quote: ... 250 μL of each sample was transferred into individual wells of a clear bottomed 96-well plate and the absorbance read at 620 nm (Multiskan FC, Thermo Fisher Scientific). Experimental glycogen concentrations were calculated off the standard curve (Herrmann & Gehringer ...
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bioRxiv - Microbiology 2024Quote: ... The plate was incubated at room temperature for 10 min after which the absorbance at 595 nm was determined (Multiskan FC, Thermo Fisher Scientific). Experimental protein concentrations were read off the standard curve (Herrmann & Gehringer ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 100 µL FACS buffer supplemented with Fc-γ receptor anti-CD16/CD32 antibodies (1:100, Thermo Fisher Scientific) and incubated on ice at 4°C for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... and resuspended in complete medium (RPMI 1640 supplemented with 10% fetal calf serum, FCS, 100 U/mL penicillin and 100 μg/mL streptomycin; Sigma-Aldrich and Invitrogen respectively).
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bioRxiv - Physiology 2023Quote: ... The formazan crystal pellets were dissolved in DMSO and the plate was read 10 min later using Multiskan FC Microplate Photometer (Thermo Fisher, USA) at 550 nm.
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bioRxiv - Immunology 2023Quote: ... Isolated T-cells were resuspended in complete RPMI (RPMI 1640 [Gibco, #21870-076] supplemented with 2% FCS and 100x Penicillin-Streptomycin [Gibco, #10378-016]).
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bioRxiv - Systems Biology 2023Quote: ... The cells were selected for three passages in DMEM supplemented with 10% FCS and 500 μg/mL G418 sulfate (ThermoFisher Scientific, 10131027). The extent of overexpression was assessed with flow cytometry (Suppl ...
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bioRxiv - Genomics 2023Quote: Lymphoblastoid cell lines (LCLs) from healthy donors and ICF2 patients were cultured in RPMI 1640 supplemented with 15% FCS and antibiotics (Thermo Fisher Scientific) as described in (15).
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bioRxiv - Cancer Biology 2023Quote: ... Uveal melanoma cell lines were maintained in RPMI and cutaneous melanoma cells and HeLa cells were cultured in DMEM supplemented with 10% FCS (Thermo Fisher Scientific) and 1% penicillin/streptomycin (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS)(AMIMED, BioConcept, Switzerland) and penicillin (100 U/ml)-streptomycin (100 µg/ml)(Gibco, Life Technologies). The MA104/NSP5-BioID2 cell line was generated using a lentiviral system ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were incubated with 5 μg/mL anti-mouse CD16/CD32 Fc block (clone 2.4G2, Thermo Fisher Scientific, Cat. #553142) for 15 minutes at 4°C per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... plus 10 % FCS (PAA Laboratories; Pasching, Austria) and P/S (100 U/ml penicillin and 100 mg/ml streptomycin; Gibco, Thermo Fisher Scientific). Results with HeLa K are shown in Fig ...
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bioRxiv - Bioengineering 2023Quote: ... TF-1 were purchased from DSMZ (Cat#ACC334) and maintained in RPMI-1640 media supplemented with 10% heat-inactivated FCS (Gibco Life Technologies), 2mM GlutaMAX and 2ng/ml hGM-CSF (215-GM ...
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bioRxiv - Developmental Biology 2023Quote: ... with 10% FCS (Biosera, #FB-1280/500) and 2% glutamate/pyruvate (100mM sodium pyruvate, Invitrogen #11360-039; 200mM L-glutamine, Invitrogen, #25030-024). Slides were imaged for brightfield ...
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bioRxiv - Cell Biology 2024Quote: ... After incubation at 37 °C for 30 minutes the mixture was transferred to a 96-well plate and turbidity was measured by absorption at 620 nm using a Multiskan FC microplate reader (Thermo Fisher, USA). All samples were examined in triplicates (n = 3).
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bioRxiv - Molecular Biology 2024Quote: ... HEK293FT and HeLa cells were cultured under standard conditions (DMEM; 10 % FCS, 1 % penicillin/streptomycin, all from Invitrogen; 37 °C; 5 % CO2). The HeLa Denr knockout (KO ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% fetal calf serum (FCS)(AMIMED, BioConcept, Switzerland) and penicillin (100 U/ml)-streptomycin (100 µg/ml)(Gibco, Life Technologies). MA/cytBirA (24 ...
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bioRxiv - Immunology 2024Quote: ... viremia samples or organ samples in 96-well plates in MEM (Sigma, M0446; supplemented with 2 % FCS and 1 % Penicilline/ Streptomycin (P/S; ThermoFisher, 15140-122)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The TMT10-plex-tagged sample (1 µg) was loaded onto an Acclaim PepMap100 C18 trap column (Thermo Fisher Scientific, 100 µm × 2 cm, 100 Å, 5 µm) at 5 µL/min using an UltiMate 3000 RSLCnano system (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: The pFAST-bac1vectors were used to produce recombinant baculovirus according to the protocol for the Bac-to-Bac system (Life Technologies). Recombinant GST-A3H Hap I WT and mutant baculovirus (K117E ...
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bioRxiv - Cell Biology 2020Quote: ... cleared lysates were mixed with Protein G/A (50:50) recombinant protein G agarose beads (catalog #15920-010, Invitrogen, Carlsbad, CA) coupled with anti-HA (catalog #3724S ...
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bioRxiv - Genetics 2021Quote: ... using 2 µg of total RNA in the presence of 2 U/µl of RNaseOut Recombinant RNase Inhibitor (Thermo Fisher Scientific). Genomic DNA was eliminated using the RapidOut DNA Removal Kit (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Recombinant bacmids were made according to instructions for the Bac-to-Bac® Baculovirus expression system given by the manufacturer (Invitrogen) using DH10EMBacY (Geneva Biotech) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Plasmid DNAs were isolated according to standard protocols and recombinant plasmids were subjected to sequencing using universal M13 primers and the BigDye terminator cycle sequencing kit v1.1 (Thermo Fisher Scientific). Sequencing products were EDTA/ethanol-precipitated ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant lentivirus was used to infect NIH3T3 cells and stably expressing cells selected with 5 μg/ml puromycin (Thermo Fisher, A1113803). Conditional expression of CB1R was induced by the addition of Doxycycline (Millipore Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... and further digested for 5 min at room temperature (22°C) using a TrypLE Express recombinant protease (ThermoFisher Scientific, cat. #12605010). The dissociated cells were sedimented by centrifugation (900 g for 10 min at 4°C) ...
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bioRxiv - Molecular Biology 2021Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was incubated with superparamagnetic beads covalently linked to recombinant protein G (Dynabeads protein G Novex, Life technologies, USA) to obtain the precleared lysate ...
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bioRxiv - Developmental Biology 2019Quote: ... Recombinant HSF2 (TP310751 Origen) was desalted (ZebaTM Spin Desalting Columns, 7K molecular-weight cutoff, 0.5 ml (1034–1164, Fisher Scientific, Germany)) and biotinylated at a molar ratio biotin/protein (3:1 ...
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bioRxiv - Cell Biology 2019Quote: ... sequence-verified plasmids (wild-type and mutant variants of pbiG2ab:6His-ZZ-SNAPf-DHC1/IC2/LIC2/Tctex1/Robl1/LC8) were used to generate recombinant baculoviral genomes by Tn7 transposition into DH10Bac cells (Life Technologies). White ...