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Citations for Thermo Fisher :
5251 - 5300 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney HEK293T cells were maintained in DMEM/F12 (Gibco) with 10 % FCS (Gibco ...
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bioRxiv - Genomics 2023Quote: Commercially available human iPSC lines derived from a female donor (Gibco Episomal hiPSC ...
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bioRxiv - Cancer Biology 2024Quote: ... added with human complete organoid medium containing Advanced DMEM/F12 (GIBCO), 10 mM HEPES (GIBCO) ...
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bioRxiv - Molecular Biology 2024Quote: Human MET knockout HeLa cells were transiently transfected with Lipofectamine3000 (Invitrogen) according to the manufacturer’s protocols in a 6-well plate format ...
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bioRxiv - Cell Biology 2024Quote: ... human custom-made 3’UTR HEC1 siRNA (oligo #3, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... miBrains were cultured in Human Endothelial Serum-Free Medium (ThermoFisher Scientific) with 1% pen/strep (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: The following human (h) and rat (r) TaqMan assays (Thermo Fisher) were used ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit anti-Human IgG DyLight™ 800 (Cat# SA5-10116, Thermofisher). For the appraisal of common painkillers drugs were as follows ...
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bioRxiv - Immunology 2024Quote: ... and human IL-6 (Gibco, PeproTech, #200-06; 20 ng/mL). Prostaglandin E2 (PGE2 ...
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bioRxiv - Immunology 2024Quote: ... and cytokine levels were measured using Human Uncoated ELISA Kits (ThermoFisher) targeting IFNψ ...
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bioRxiv - Developmental Biology 2024Quote: ... Complete human medium with 10% exosome depleted FBS (Gibco, Massachusetts, US) was replenished and device placed into incubator ...
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bioRxiv - Cell Biology 2024Quote: ... Human Pools Set v3.0 kit (Applied BiosystemsTM, Thermo Fisher Scientific, USA) and the TaqManTM MicroRNA Reverse Transcription Kit (Applied BiosystemsTM ...
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bioRxiv - Systems Biology 2024Quote: ... Human THP-1 monocytes were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... Normal human epithelial cells were cultured in keratinocyte-SFM medium (Gibco) supplemented with 1% antibiotic-antimycotic (Gibco).
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bioRxiv - Genomics 2024Quote: We used the human Amyloid-β 40 (ThermoFisher Cat No. KHB3481), Amyloid-β 42 (ThermoFisher Cat No ...
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bioRxiv - Immunology 2024Quote: ... stimulated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco™) and cultured for 5 days in AIMV medium supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Plant Biology 2019Quote: ... Aliquots were analyzed on a 10% SDS-PAGE followed by Coomassie Blue staining or Western blot analysis with goat α-6X-His-HRP antibody (Life Technologies, Carlsbad, CA) at a 1:5,000 dilution in Tris-buffered saline (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The gene optimized for the Drosophila codon usage and extended by C-terminal His-tag was purchased from Life Technologies (DNA String fragments, USA). The DNA fragment was inserted into a pMT-BiP-V5-His_A vector (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: 1-2 μg of purified 6xHis-tagged calcineurin or 6xHis-MBP as a negative control was first bound to 10 μL magnetic Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Fisher Sci., 10104D) in 490 μL base buffer (50 mM Tris-HCl pH 7.5,150 mM NaCl ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by sequencing using the Ion PI™ Hi-Q™ Sequencing 200 Kit on Ion Proton PI™ V2 chips (Thermo Fisher Scientific), an Ion Proton™ System ...
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bioRxiv - Genetics 2020Quote: ... in all the above clonings except for the his-72::GFP repair template which required ccdB resistant bacteria for which we used One Shot ccdB Survival (Thermo Fisher Scientific, cat. A10460). DNA extractions by miniprep were done with the ZymoPURE Plasmid Miniprep kit and elution with water (Zymo Research ...
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bioRxiv - Immunology 2020Quote: ... Nunc MaxiSorp ELISA plates were coated with 50 μL of capture antibody (anti-6x-His antibody clone HIS.H8; 2 μg/mL; Thermo Fisher Scientific, Waltham, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR product of the C-His oipA gene fragment was cloned into vector pLATE11 using aLICator LIC Cloning and Expression Kit 1 (Thermo Fisher Scientific, United States) by following the kit’s instructions ...
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bioRxiv - Pathology 2023Quote: ... The cDNA was used for RT-qPCR with qPCRBIO SyGreen Blue mix Hi-ROX (PCR Biosystems, London, UK) on the StepOne Plus Real-Time PCR machine (Applied Biosystems, Foster City, CA) and primers listed in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... Protein bands were visualized with Coomassie staining (ROTIBlue Quick, Carl Roth) or immunodetected with a monoclonal anti-His antibody conjugated to HRP (MA1-21315-HRP, Thermo Fisher, 1:2000 diluted). A detailed protocol can be found in protocols.io (dx.doi.org/10.17504/protocols.io.bu67nzhn).
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bioRxiv - Genomics 2023Quote: ... Vero/hSLAM and VeroE6/TMPRSS2 were maintained in supplemented DMEM with 10% HI-FBS and 1 mg/mL G418 sulfate (Thermo Fisher 10-131-027). HeLa/ACE2/TMPRSS2 cells were a generous gift from Dr ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% (v/v) heat-inactivated (HI) FBS (Thermo Fisher Scientific, Waltham, MA, USA). Stable cell lines expressing F-CIC-S ...
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bioRxiv - Biophysics 2020Quote: ... The confocal parameters were calibrated daily by measuring the FCS decay of a 20 nM TAMRA (carboxylic acid of tetramethyl rhodamine) free dye solution (Molecular Probes, Inc.) with a known diffusion coefficient (D = 420 μm2s−1 ...
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bioRxiv - Cell Biology 2020Quote: ... FCS (10% FCS-DMEM/F-12) at 37°C under 5% CO2/95% air in a Heracell 150i CO2 incubator (Thermo Fisher Scientific). Cells were passaged every 2 days or once they had reached 80% confluency ...
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were incubated with 5 μg/mL anti-mouse CD16/CD32 Fc block (clone 2.4G2, Thermo Fisher Scientific, Cat. #553142) for 15 minutes at 4°C per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 1:100 human IgG (1 mg/ml) as FcR block and 2 % FCS using the following staining reagents: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Optical density of standards and samples were measured at OD595 nm using a Multiskan™ FC Microplate Absorbance Reader (Thermo Scientific, Belgium). Plasma and cell lysate were prepared by mixing 100 µl citrated plasma with 100 µl RIPA buffer and 6 × 106 HUVECs with 250 µl RIPA ...
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bioRxiv - Microbiology 2022Quote: Expression plasmids encoding CLII or CLIV with an Fc- and His6-tag were transfected in Expi293F expression cells (Gibco-Thermo Fischer Scientific) using the ExpiFectamine293 transfection kit ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cells were harvested by centrifugation (500 x g, 5 min) and washed with CO2-independent medium supplemented with 10 % FCS (Thermo Fisher Scientific). Afterwards ...
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bioRxiv - Genomics 2019Quote: NIH3T3 and HEK293FT cells were cultured under standard conditions (DMEM; 10% FCS, 1% penicillin/streptomycin, all from Invitrogen; 37°C; 5% CO2).
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bioRxiv - Immunology 2021Quote: ... immune cells in the 70-30% interphase were collected and washed twice with RP10 (RPMI 1640, Thermo Fisher; 10% FCS, 1% penicillin/streptomycin (Invitrogen, #15140122) 1% glutamine (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... NKG2DL expression was assessed by staining with NKG2D-Fc chimera (10 µg / mL; Fisher 1299NK050) followed by an αFc secondary stain (Invitrogen #A-10631). NIR Live/Dead (ThermoFisher #L34976) ...
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bioRxiv - Cell Biology 2021Quote: ... Akata cells were grown at 37°C under 5% CO2 in RPMI-1640 supplemented with 10% FCS and 2 mM of L-Glutamine (Gibco, 25030-081). Cells KD for BHRF1 were generated from Akata cells (see below ...
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bioRxiv - Cell Biology 2021Quote: ... and A9 cells (obtained from Nobuo Takagi at Hokkaido University in 1980s) were cultured in DMEM (Nacalai Tesque) with 10% FCS (Thermo Fisher Scientific) and 1% GPS solution (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with heat-inactivated 10% v/v foetal calf serum (FCS; HyClone) and 500 μg/mL Geneticin (Gibco cat no 10131-0275) and kept under 5% CO2 at 37°C.
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bioRxiv - Cell Biology 2020Quote: BM cells of C57Bl/6 donor mice were collected by flushing tibias and femurs by using 10 mL syringes (Terumo) filled with cell culture medium (DMEM, 10% FCS, 1% Penicillin-Streptomycin, 1% Antibiotic-Antimycotic, Thermo Fisher Scientific) (Kruger et al. ...
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bioRxiv - Bioengineering 2022Quote: ... The absorbance at a wavelength of 450 nm was measured using Multiskan™ FC Microplate Photometer (Thermo Fisher Scientific, Waltham, MA USA).
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bioRxiv - Cancer Biology 2022Quote: ... Protein bands were visualized using a LI-COR Biosciences Odyssey FC imaging system after the addition of SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific). Proteins were quantified with Image Studio Version 5.2.4 (LI-COR Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: Whole lung single cell suspensions were passed through a 40 µm cell strainer and preincubated with anti-mouse CD16/CD32 Fc block (1:100, Thermo Fisher Scientific) for 15 min in flow buffer (PBS supplemented with 5% (vol/vol ...
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bioRxiv - Neuroscience 2021Quote: ... Sendai virus-infected primary fibroblasts were immediately centrifuged for 45 min at 32 °C with 1,500 g (spinfection) and cultivated in Advanced DMEM containing 5% fetal calf serum (FCS) and 1% GlutaMAX™ (all from Life Technologies). On the following day the viruscontaining medium was replaced with fresh culture medium ...
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bioRxiv - Bioengineering 2020Quote: ... Pro + using an optimized autoinduction media and purified by protein A affinity chromatography similarly to Fabs.30 VH-Fc were expressed in Expi293 BirA cells using transient transfection (Expifectamine, Thermo Fisher Scientific). 4 days after transfection ...
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bioRxiv - Immunology 2022Quote: ... were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024], 1 mM sodium pyruvate [Gibco Life Technologies ...