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Citations for Thermo Fisher :
5251 - 5300 of 10000+ citations for 2 4 Difluoro N 2 methoxy 5 4 4 morpholinyl 6 quinazolinyl 3 pyridinyl benzenesulfonamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... 5 µL of the lysates were resolved on a NuPAGE 4-12% Bis-Tris Protein Gel with MOPS buffer (Thermo Scientific) and transferred to a 0.45 µm nitrocellulose membrane (Cytiva ...
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bioRxiv - Genetics 2024Quote: ... Samples were heated at 95°C for 5 min (protein denaturation step) before loading onto a 4–12% NuPAGE precast gel for Western analysis (ThermoFisher Scientific). After the transfer to PVDF membranes (LC2005 ...
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bioRxiv - Biophysics 2024Quote: ... 1 µL of the completed PCR reactions was then combined with 4 µL of water and 5 µL of print buffer (2x: 20 mg/mL UltraPure BSA (Thermo Fisher), 24 mg/mL trehalose dihydrate ...
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bioRxiv - Cancer Biology 2024Quote: ... Radiation-induced alterations in ZFE length were measured at 5 days post-fertilization (dpf) following embryo fixation (4% PFA) and microscopic imaging (Evos XL Core Cell Imaging System, Thermo Fisher). Analysis was conducted using ImageJ software ...
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bioRxiv - Cell Biology 2024Quote: ... At day 4 and 5 media was replaced with Endothelial induction media composed by StemPro-34 SFM Media (10639011, Life Technologies) with StemPro-34 supplement ...
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bioRxiv - Biophysics 2024Quote: ... The cells were allowed to attach to the grids for 4-5 h before vitrification in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific). The following settings were used ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were centrifuged at 1000 rpm for 5 min at 4°C and resuspended in FACS buffer containing Alexa Fluor 488 goat anti-mouse IgG (Invitrogen, #A11001) at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples of ∼ 30 µg protein prepared with (reducing) 5% β-mercaptoethanol were separated on a 4-12% Bis-Tris NuPAGE gels (Invitrogen) and transferred to polyvinylidene-fluoride membrane for 1 h at 4°C at 40 V ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell solutions were added to cytofunnels and the samples were spun for 5 mins at 700 RPM in a Cytospin 4 centrifuge (Thermo Fisher). The slides were air-dried overnight at RT in a vertical position and the next day the cell smears were circled with a hydrophobic pen (Abcam #ab26-01 ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were mixed with 5 µg antibody and incubated at 4°C for overnight followed by incubation with Dynabeads protein G (Life Technologies) for 4 h at 4°C ...
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Trade-offs between antiseptic cytotoxicity and efficacy in a human ex vivo wound contamination modelbioRxiv - Microbiology 2021Quote: ... each bisect was rinsed in PBS and placed in an individual well of a 6-well plate with 2 mL MTT solution (2 mg/mL, Invitrogen) in each well ...
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bioRxiv - Cancer Biology 2020Quote: ... 300,000 cells in 6-well plate were transfected for 48 h with 2 µg of plasmid DNA using Lipofectamine 2 000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... Insoluble fraction was removed by high speed centrifugation at 4°C (25658 g for 30 minutes, Thermo Fisher SCIENTIFIC #A23-6 x 100 rotor). The supernatant was filtrated with 0.22 μm filter (Filtropur BT50 0.2 ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (Invitrogen 4’,6-diamidino2-phenylindole). Photomicrographs of immunofluorescence staining were taken using a Leica DMRA fluorescence microscope with a Leica DFC360 FX camera (Leica ...
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bioRxiv - Bioengineering 2022Quote: ... Devices were washed with PBS for 4-6 hrs and subsequently incubated with Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) (ThermoFisher cat# A32728, 1:300) and Hoechst 33342 (Sigma-Aldrich cat# B2261 ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed hearts were embedded in paraffin and sections (4-6 μm thickness) were mounted onto Superfrost® Plus microscope slides (Thermo scientific #630-0950). Immunohistochemistry was carried out using standard procedures ...
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bioRxiv - Biochemistry 2022Quote: ... The insoluble fraction was removed by high-speed centrifugation at 4°C (25658 g for 30 minutes, A23-6 x 100 rotor, (Thermo Fisher Scientific, Waltham, MA). The supernatant was filtrated with a Filtropur BT50 0.2 µm bottle top filter (SARSTEDT ...
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bioRxiv - Bioengineering 2022Quote: ... Rat bone marrow mononuclear cells were cultured at 4-6 million in a 10cm dish (Falcon) using differentiation media containing: DMEM (ThermoFisher Scientific, Burlington, ON, Canada), 20% HI FBS ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before being flash frozen in liquid nitrogen ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before total RNA was isolated from cells by Purelink™ RNA Mini Kit (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... determined by preparing a slurry with 1 part soil to 2 parts deionized water and measuring (n=3) with an Orion Star A111 pH Meter (Thermo Scientific, Waltham, MA, USA), was 5.87 ± 0.42 in November 2020 and 6.28 ± 0.07 in April 2021.
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bioRxiv - Biophysics 2021Quote: ... Then 2 equivalents of fluorescein-5-maleimide (Invitrogen, #F150) was added and the mixture was incubated at room temperature for 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... EdU (5-ethynyl-2’-deoxyuridine, ThermoFisher Scientific, cat# A10044) staining was performed by Click-it Alexa Fluor 647 imaging kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and 5 ng/ml IL-2 (Thermo Fisher Scientific), and then resuspended with 1 ml of virus per 6 x 106 cells ...
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bioRxiv - Cell Biology 2021Quote: 0.1mg of 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen, A10044) dissolved in PBS (50ul ...
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bioRxiv - Developmental Biology 2020Quote: 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen A10044, Carlsbad, CA) dissolved in PBS was administered to mice at indicated postnatal days ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μM 5-ethynyl-2’-dexoyuridine (EdU, ThermoFisher Scientific) was added to the culture media for 2 hours ...
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bioRxiv - Developmental Biology 2021Quote: 5-ethynyl-2′-deoxyuridine (EdU) was purchased from ThermoFisher and stock solutions were created by dissolving the powder in dimethyl sulfoxide as instructed by the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU, Molecular Probes, Life Technologies) was dissolved in DMSO to make a 10mM stock solution ...
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bioRxiv - Cancer Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU, Molecular Probes, Life Technologies) was dissolved in DMSO to make a 10mM stock solution ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 5% FCS and 2% B-27 (Gibco) and maintained at 5% CO2 and 37°C in humidified incubators ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μg plasmid and 5 μl Lipofectamine 2000 (Invitrogen) were diluted into 200 μl Opti-MEM (Gibco ...
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bioRxiv - Genomics 2022Quote: EdU (5-ethynyl-2’-deoxyuridine) (Thermo Fisher Scientific, A10044) was added to culture media at 10 µM final concentration for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μl of 5 × Maxima RTA buffer (Thermo Scientific), which were mixed with Ultrapure water (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... 2-amino-5-methoxybenzoic acid 1 M (ThermoFisher Scientific) in DMSO ...
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bioRxiv - Neuroscience 2024Quote: EdU (5-ethynyl-2’-deoxyuridine; #A10044, Thermo Fisher Scientific) was diluted at 10mg/ml in 0.9% NaCl (#114-055-101 ...
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bioRxiv - Neuroscience 2024Quote: EdU (5-ethynyl-2’-deoxyuridine; #A10044, Thermo Fisher Scientific) was diluted at 10mg/ml in 0.9% NaCl (#114-055-101 ...
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bioRxiv - Cancer Biology 2024Quote: ... EdU (5-ethynyl-2’-deoxyuridne) (Thermo Fisher Scientific, #A10044) for 4 hours after incubating cells with serum-deprived media (RPMI +10% FBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or SM/5 plates (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Bioengineering 2020Quote: ... N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide HCl (50-848-678, Fisher Scientific), butyric acid (B103500-100ML ...
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bioRxiv - Physiology 2020Quote: ... 0.05% 3-(N,N-Dimethylmyristylammonio) propanesulfonate zwittergent detergent (Acros Organics, 427740050)) containing protease inhibitor (Mammalian ProteaseArrest-APExBIO K1008) ...
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bioRxiv - Microbiology 2020Quote: ... Tryptic peptides (2 μg) were loaded onto a C18 trap (75 um x 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 ul/min of solvent A (0.1% FA and 3% acetonitrile in LC-MS grade water) ...
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bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
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bioRxiv - Cell Biology 2024Quote: ... Tryptic peptides (2 µg) were loaded onto a C18 trap (75 µm × 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 µL/min of solvent A (0.1% formic acid and 3% acetonitrile in LC-mass spectrometry grade water) ...
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bioRxiv - Systems Biology 2024Quote: ... Tryptic peptides (2 μ were loaded onto a C18 trap (75 um x 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Scientific) at a flow rate of 2μl/min of solvent A (0.1% formic acid in LC-MS grade water) ...
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bioRxiv - Microbiology 2021Quote: ... ACMA stands for 9-amino-6-chloro-2-methoxyacridine (A1324, ThermoFisher Scientific). For each measurement in the spectrofluorometer (Hitachi F-7000) ...
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bioRxiv - Microbiology 2021Quote: ... 6- diamidino-2-phenylindole dihydrochloride (DAPI; Thermo Fisher D1306; 1:1,000 dilution) for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... in blocking buffer containing 4ʹ,6-diamidino-2-phenylindole (DAPI; 1;1,000, Invitrogen). After staining ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclei (DNA) were stained with 6-diamidino-2-phenylindole dihydrochloride (DAPI, Invitrogen). Slides were embedded with Mowiol (0.33 g/ml glycerol (Sigma-Aldrich 15523-1L-R) ...
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bioRxiv - Neuroscience 2020Quote: ... 6-diamidino-2-phenylindole (DAPI, 50 μg/ml, D1306; Thermo Fisher Sci) was added to visualize the cell nuclei ...