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Citations for Thermo Fisher :
5201 - 5250 of 10000+ citations for 2 4 Difluoro N 2 methoxy 5 4 4 morpholinyl 6 quinazolinyl 3 pyridinyl benzenesulfonamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... the samples were transferred to the dialysis wells plates and incubated at 37°C and 5% CO2 for a total 4 weeks in osteogenic monoculture medium containing DMEM (low glucose, 22320, Thermo Scientific), 10% human platelet lysate [11] (hPL ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... the neurons were given 12 hours in growth media at 37°C/5% CO2 to allow sufficient recovery and expression of the plasmid prior to fixation in 4% formaldehyde (Thermo Fisher) / 4% sucrose (VWR ...
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bioRxiv - Cancer Biology 2023Quote: ... 5-20 μg of total protein was separated by NuPAGE 4-12% Bis-Tris Mini Protein Gels (NP0336BOX; Thermo Fisher Scientific) and transferred to Immobilon-P PVDF Membrane (IPVH00010 ...
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bioRxiv - Genomics 2024Quote: ... The purified DNA solutions from the eight aliquots were combined and loaded into 5 lanes of 4% E-gel (Invitrogen, G401004). The DNA bands with the size between 300 bp and 1.2 Kb were excised ...
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bioRxiv - Immunology 2024Quote: ... Lysates were then incubated overnight at 4 °C with 5 μg of prebound anti-SP100 or rabbit IgG isotype control protein A dynabeads (Thermo Fisher). The next morning ...
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bioRxiv - Immunology 2023Quote: ... at 1:100 overnight at 4°C in 5% BSA in the presence of 0.05% sodium azide (BP922I-500; Thermo Fisher Scientific), 0.5% Triton X-100 (161-0407 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspensions were centrifuged at 400 g for 5 min at 4 °C with no brake and resuspended in staining buffer (DPBS (without Ca2+ and Mg2+) (Gibco, Life Technologies) containing 0.1 % low endotoxin BSA (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: Protein extracts were boiled with NuPAGE 4x sample buffer containing 50 mM DTT for 5 min and then loaded onto NuPAGE Novex 4-12% Bis-Tris gels (ThermoFisher Scientific). Electrophoresis was performed according to a standard protocol followed by transfer of proteins onto PVDF membrane (pore size 0.2 µm ...
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bioRxiv - Immunology 2023Quote: ... 20 ng ml-1 GM-CSF (Biozym) or X38-Ag8 supernatant (1%) and from day 5 on additionally with 10 ng ml-1 IL-4 (Life technologies). At day 7 ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL of eluted ChIP-DNA were mixed with 5 µL of PowerUp SYBR Green master mix (Applied Biosystems, cat#A25743), and 1 µL of the primer mix (forward+reverse ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95°C for 5 min and proteins resolved on 4-20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Differentiation medium was replaced daily on days 4 and 5 by 75:25 KSR:N2 medium (486.5 mL DMEM/F-12 (Thermo Fisher Scientific, 11320033), 5 mL 15 % glucose ...
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bioRxiv - Immunology 2023Quote: ... boiled for 5 min at 95°C and separated on a 4-12% NuPAGE Novex Bis-Tris precast gel (Thermo Scientific).
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bioRxiv - Neuroscience 2023Quote: ... or Tris-Glycine-SDS sample buffer (Novex) at 95°C for 5 min and separated using 4–20% Tris-glycine (ThermoFisher, #EC6025 or Invitrogen # XP04200). Proteins were transferred to nitrocellulose membranes (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Cell suspension was centrifuged at 500 g for 5 minutes at 4°C and the pellet resuspended in 0.5% BSA (A1391 PanReac AppliChem) in DPBS (14190250 Thermo Fisher Scientific) containing 1mM EDTA (E177 VWR Life Science ...
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bioRxiv - Immunology 2023Quote: ... cells were pelleted at 100 x g for 5 min (4°C) and then again digested with TrypLE Express Enzyme (Gibco, 12604013) for 15 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 50,000 cells swollen after hypotonic shock in 200 μl of KCl 75 mM were cytospun on a slide for 5 min at 900 rpm (Cytospin 4, Thermo Fisher). These cells were then fixed in 3% paraformaldehyde/PBS for further immunolabelling.
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bioRxiv - Cell Biology 2023Quote: ... ESCs (5 × 10^4 cells/well) were dissociated into single cells with 0.5mM EDTA (15575020, Gibco; Thermo Fisher Scientific, MA, USA) after exposure to ROCK inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... ESCs (5 × 10^4 cells/well) were dissociated into single cells with 0.5mM EDTA (15575020, Gibco; Thermo Fisher Scientific, MA, USA) after exposure to ROCK inhibitor ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged at circa 70-80% confluency or every 4 or 5 days using Accutase (7002353, Thermo Fisher / Life Technologies). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged at circa 70-80% confluency or every 4 or 5 days using Accutase (7002353, Thermo Fisher / Life Technologies). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... incubated in secondary antibody solution for 4 hours at RT (5% normal goat serum in PBST, Alexa Fluor 633 anti-guinea pig 1:200 (Thermo Scientific)) ...
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bioRxiv - Cancer Biology 2023Quote: ... a final extension step (68°C for 5 min) and holding the reaction at 4°C in a thermocycler (Applied Biosystems) with a preheated lid (105°C) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... in 5% FBS overnight at 4°C and Alexa Fluor® 488 goat anti-mouse IgG secondary antibody (1:200; #10696113, ThermoFisher) for one hour at RT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the samples were run on a thermocycler (60 min, 37°C; 5 min, 95°C; held, 4°C; Applied Biosystems); cDNA was stored at −20°C until use.
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bioRxiv - Genomics 2023Quote: ... plugs were washed 4 times in 1×Wash Buffer (BNG) and 5 times in 1× TE Buffer (ThermoFisher Scientific, Waltham, MA). Then ...
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bioRxiv - Microbiology 2023Quote: ... Blocking was performed for 1h (5% skimmed milk in Tris-buffered saline containing 0.5% Tween) and primary antibody staining was carried out overnight at 4°C (polyclonal anti-PBP2a, custom antibody from Thermo Fisher, 1:1000 or polyclonal anti-Sortase A ...
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bioRxiv - Cell Biology 2023Quote: Immunohistochemistry and Immunofluorescence were performed on 4% PFA-fixed paraffin-embedded 5-μm sections mounted onto Super frost microscope slides (ThermoFisher Scientific) or whole mounts of denuded oocytes ...
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bioRxiv - Cell Biology 2023Quote: ... 4 and 5 we confined cells by using a soft acrylamide gel and either 1% Low Melting Agarose (Invitrogen, 16520-100) or a second soft acrylamide gel ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were incubated for 5 min at 99°C before loading on 4-12 % SDS-PAGE with MOPS as running buffer (Thermo-Fisher Life Technologies). BenchMark protein ladder was used as marker (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed again at 1224 rpm for 5 minutes at 4 degrees Celsius and then counted using an automated cell counter (Countess, Thermo Fisher) to obtain the cell concentrations ...
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bioRxiv - Microbiology 2023Quote: ... the media from each well was aspirated and the wells were overlaid with 4 ml/well DMEM with 5% FBS and 0.7% agarose (Invitrogen, 16500-500). At 7 dpi ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were washed again at 1224 rpm for 5 minutes at 4 degrees Celsius and then counted using an automated cell counter (Countess, Thermo Fisher) to obtain the cell concentrations ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were subsequently permeabilized with 0.5% Triton solution for 5 min at 4°C and then incubated with PicoGreen (1:1000 dilution) (Cat No: P11496, ThermoFisher Scientific) in PBST buffer (PBS plus 0.1% Tween-20 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein isolates were mixed with protein loading buffer containing 100 mM DTT reducing agent and denatured for 5 min at 95°C before loading onto 4–12% Bis-Tris NuPAGE precast gels (Thermo Fisher) for electrophoresis ...
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bioRxiv - Systems Biology 2024Quote: ... about 10 cm of leaf length were cut into small pieces and placed in chilled 5-mL tubes containing 4 mL of RNALater (Fisher Scientific) solution for RNA stabilization and storage ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting cell suspension was passed through a 70-μm filter, centrifuged (400xg, 5 minutes, 4 °C) and resuspended in Advanced DMEM/F12 (ThermoFisher #12634010) supplemented with FCS (10% vol/vol ...
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bioRxiv - Neuroscience 2024Quote: ... The RNase I treatment was carried out as described by Ingolia et al.19 for 45 min at 4 °C using 5 µl of RNase I (Ambion, AM2295) per 250 µg of crude total RNA and quenched by adding 20 µl of SUPERase-In (Ambion ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were washed with 5 mL PBS (4°C) and trypsinized with 1 mL 0.25% Trypsin-EDTA per dish (Gibco, 25200-056) for 5 min at 37°C and 5% CO2 ...
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bioRxiv - Physiology 2024Quote: Intracellular Ca2+ [Ca2+]i measurements were performed in cardiomyocytes loaded with Fluo-4 AM dye (5 μmol.L-1, ThermoFisher, Cat #F14201). After a 30-minute loading period ...
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bioRxiv - Neuroscience 2024Quote: ... The protein mixture was boiled at 95 °C for 5 minutes before run on a 4-12 % Bis-Tris gel (Invitrogen, #NP0335BOX) in NuPAGE MOPS buffer (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... cells were lysed with 23G needles and the parasites passed through a 5 µm filter before fixation in 4% PFA and staining with Hoechst 33342 (Thermofisher Scientific). Samples were analysed on a BD LSR Fortessa flow cytometer and with FlowJo software v10 ...
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bioRxiv - Biophysics 2024Quote: Aliquots of 3 μL of complex sample was applied on UltrAuFoil r1.2/1.3 Grids covered with Graphene Oxide as described before76 and the grids were blotted for 3.5 s at force -1 at 4°C and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 26,386 Movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were heated at 95°C for 5 min and proteins resolved on 4–20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... 5 µL of the lysates were resolved on a NuPAGE 4-12% Bis-Tris Protein Gel with MOPS buffer (Thermo Scientific) and transferred to a 0.45 µm nitrocellulose membrane (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... murine lung or spleen cells were stimulated for 4 hrs with Ancestral spike (5 μg/mL) and then supplemented with Protein Transport Inhibitor cocktail (Life Technologies) for a further 10-12 hrs ...
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bioRxiv - Immunology 2024Quote: ... 35 μg of protein lysate was heated at 95 °C for 5 min and loaded onto Novex Tris-Glycine Mini-Protein Gel 4–12% (ThermoFisher #XP04200BOX) and run at 100V for 1.5 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the samples were run on a thermocycler (60 min, 37 °C; 5 min, 95 °C; held, 4 °C; Applied Biosystems); cDNA was stored at −20 °C until use.
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bioRxiv - Neuroscience 2024Quote: LDS-treated samples were thawed and boiled at 95°C for 5 minutes before loading into 1.0 mm-thick Bis-Tris 4-12% polyacrylamide gels (Invitrogen, cat# NP0323) and subjected to electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... The fixed cells were spun down at 900 × g for 5 min at 4°C and then washed twice with 1 ml of ice-cold PBS (Gibco 70011044). Cells were lysed twice with 1 ml of ice-cold 4C permeabilization buffer (50 mM Tris-HCl pH 7.5 ...