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Citations for Thermo Fisher :
5151 - 5200 of 10000+ citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... These primary antibodies were subsequently detected by incubation with appropriate Alexa Fluor 488 or 594 conjugated goat antibodies (1:250; Invitrogen). Images were acquired using a Leica TCS STED SP8 confocal microscope or a Zeiss LSM800 confocal microscope with the same settings for all samples within one experimental group ...
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bioRxiv - Microbiology 2022Quote: ... E and S1 exposure were conducted in the presence or absence of neutralizing antibodies against toll-like receptor 2 and 4 (TLR2 and TLR4) antibodies (ThermoFisher Scientific Cat ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with a NPC1 primary antibody (1:70) followed by a DY650 secondary antibody (1:400) and mounted with PermaFluor Aqueous Mounting medium (ThermoFisher). Imaging was performed with a LSM800 confocal microscope (AxioObserver Z1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were rinsed and then incubated with fluorophore-conjugated secondary antibody at 1:300 dilution (Alexa Fluor 488- or Alexa Fluor 594-conjugated polyclonal antibody, Invitrogen) and DAPI in blocking buffer for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were rinsed and then incubated with fluorophore-conjugated secondary antibody at 1:300 dilution (Alexa Fluor 488- or Alexa Fluor 594-conjugated polyclonal antibody; Invitrogen) and 4l,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2022Quote: ... and with and Immunofluorescence using anti-rabbit GFP antibody (a gift from Jason Mercer, University of Birmingham, UK) and secondary anti-rabbit Alexa Fluor 488 antibody (Invitrogen). Nuclear staining was done by incubating the sections in Hoechst 33342 (Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... cultures were incubated in rabbit anti-RIM 1/2 antibody (1:200) followed by donkey anti-rabbit AF647 antibody (1:500; Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... for 1hr before being incubated overnight at 4°C in and primary antibodies (1:1000 mouse anti-V5 Tag monoclonal Antibody (Invitrogen) and anti-Actin monoclonal antibody (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... 4 μL of either mouse anti-TG2 antibody (Novus NBP2-26458) or hamster anti-Zbtb7a antibody (Invitrogen 14-3309-82) was added to each sample ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated with the primary antibody at 4°C overnight and then with Alexa Fluor-conjugated secondary antibody (Invitrogen). Images were obtained using a Zeiss Axio Imager M2 microscope ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed 3 times in TBS for 10 min each and incubated for 1 h at room temperature with a combination of 2 fluorescent dye-labelled secondary antibodies: a Cy3-conjugated goat anti-rabbit antibody (1:200; Invitrogen, Waltham ...
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bioRxiv - Microbiology 2022Quote: ... Spike protein was detected by primary antibody (1:1500, Sinobiological) followed by Alexa Fluor 647 anti-rabbit secondary antibody (1:1000, Invitrogen). The nucleus was labelled using DAPI (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no.A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
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bioRxiv - Physiology 2022Quote: ... Hearts were then washed trice in PBS for 20 minutes and incubated with the secondary antibody (donkey anti-rabbit antibody conjugated to Alexa 555; A-31572, Invitrogen) that was diluted 1:100 for at least 2 days at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... Blots were washed with TBST 3x for 5 min and incubated in corresponding secondary antibody for 1 hour room temperature: goat anti-rabbit antibody (Invitrogen) or rabbit anti-mouse antibody (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit monoclonal antibodies against VEGFR2 (1:200, clone 55b11, Cell Signalling) and rabbit polyclonal antibodies against COL15A1 (1:200, Invitrogen) was performed ...
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bioRxiv - Pathology 2024Quote: ... Antibodies to TOM70 (14528-1-AP, 1:200, Proteintech; Alexa Fluor 488-conjugated secondary antibody: A-11034, 1:500, Invitrogen), and troponin T (1:100 ...
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bioRxiv - Pathology 2024Quote: ... were stained in LV cryosections of 10 µm using a rabbit antibody to TOM70 (14528-1-AP, 1:200, Proteintech; Alexa Fluor 488-conjugated secondary antibody: A-11034, 1:500, Invitrogen) and a mouse Alexa Fluor 647-conjugated antibody to TnT (#565744 ...
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bioRxiv - Plant Biology 2024Quote: ... bound antibodies were detected using a goat anti-rabbit secondary antibody coupled to a fluorescent molecule (Alexa Fluor 680, Invitrogen) diluted 1:10,000 using the “Odyssey Infrared Imager” at 680 nm (Licor ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3 times with PBS, followed by incubation in secondary antibodies (goat antibodies conjugated with Alexa Fluor 488, 546 or 647 (Invitrogen)) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Cells were blocked in PBS + 10% normal goat serum and incubated with anti-Pan-CEACAM antibody followed by goat anti-mouse AF555-conjugated antibody (Invitrogen). Cells were analyzed using imaging flow cytometry and IDEAS software ...
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bioRxiv - Microbiology 2024Quote: ... The IMC protein GAP45 was labeled using the anti-GAP45 rabbit antibody (1:5000) (a kind gift from Julian Rayner) and goat anti-rabbit AlexaFluor 488 antibody (1:500, Invitrogen). Alfa-tubulin was labeled with an anti-alfa tubulin mouse antibody (1.500 ...
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bioRxiv - Neuroscience 2024Quote: ... Visualizations of the primary antibodies were achieved using suitable secondary antibodies conjugated with Alexa fluorophores (Alexa Fluor 488, 594, and 647, Invitrogen).
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bioRxiv - Physiology 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 10% goat serum and antibodies against laminin β1 (1:100; MA5-14657, Invitrogen) or laminin β1γ1 (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were use: AF647 polyclonal goat anti-mouse IgG2a antibody and AF488 Donkey anti Rabbit IgG (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with one of the following secondary antibodies at RT for 2 hours: anti-rabbit Alexa Flour-488 conjugated antibody (Cat# A-11008, Invitrogen), anti-rabbit Alexa Flour-555 conjugated antibody (Cat# A-21428) ...
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bioRxiv - Neuroscience 2024Quote: ... The following secondary antibodies were used in the study: donkey anti-goat antibody Alexa Fluor 647 (1:200, Invitrogen™), donkey anti-rabbit Alexa Fluor 546 (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane probed with an anti-transthyretin antibody was further incubated with goat anti-rabbit secondary antibody (1:1,000; Invitrogen 31460). The membrane was washed three times for ten minutes and then incubated with an enhanced chemiluminescence reagent (Promega W1001) ...
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bioRxiv - Genomics 2024Quote: ... The sample was stained with a secondary antibody using the same protocol as the primary antibody (3.75 µg/ml; ThermoFisher; A16112). The secondary antibody was labeled with an oligonucleotide using previous protocols45.
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bioRxiv - Neuroscience 2024Quote: ... Antibodies were labeled with appropriate host species secondary antibodies (Table S5): donkey anti-mouse IgG conjugated to Alexa 488 (Thermofisher, Cat No ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated for 2h with the secondary antibody (Alexa Fluor 488-conjugated donkey anti-mouse antibody, 1:1000; Invitrogen). Finally ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibody binding was detected by a goat-anti-rabbit antibody conjugated to horseradish peroxidase (HRP) (1:2000 dilution; ThermoFisher). Signals were detected using a chemiluminescence substrate and the Azure c600 Imaging System (Azure Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... Infected cells were then fixed for 20 min in 10% formalin and processed for immunofluorescence with 1 μg/ml mouse anti-RhCMV pp65B or 5 ug/ml mouse anti-Rh152/151 monoclonal antibody followed by a 1:500 dilution of goat anti-mouse IgG-Alexa Fluor 488 antibody (Invitrogen). Nuclei were stained with DAPI for 10 min (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibodies were incubated for 1 h followed by washes and incubation with Alexa Fluor conjugated secondary antibodies (Thermofisher Scientific) for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... and anti-Tip60 polyclonal antibodies for 1 hour at room temperature followed by secondary antibodies conjugated to Alexa488 and Alexa 568 (Invitrogen). The cells were stained with 0.1 μg/ml of DAPI (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... slices were stained with fluorescent secondary antibodies (Alexa Fluor 488 goat anti-rabbit secondary antibody, A-11008, Invitrogen, RRID: AB_143165) and DAPI at 1:500 dilution at 4°C for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... primary antibody for 1 h followed by Alexa Fluor 647 goat anti-rabbit secondary antibody (1:1000; Cat. A21245, Invitrogen) for 1 h to label the CCVs ...
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibodies used included Alexa Fluor 488-conjugated donkey anti-mouse IgG antibody (1:500, #A-21202, ThermoFisher Scientific), Alexa Fluor 488-conjugated donkey anti-rat IgG antibody (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... were then subjected to preclearing with 20 μL of either magnetic protein G (for Rabbit antibodies) or Protein A (for Mouse antibodies) Dynabeads (Invitrogen) for 1 hour at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody incubation was carried out overnight at 4 °C with human-CD68 monoclonal antibody (cat. No. 14-0688-82, Invitrogen) and CD19 Rabbit polyclonal antibody (cat ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... incubated with primary antibody (anti-human ASCT2 from MedImmune diluted 1:3000 in FACS buffer) for 30 min and secondary antibody for 20 min (Goat anti-human IgG Fc PE secondary antibody (Invitrogen) diluted 1:250 in FACS buffer) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated with primary antibodies for 2 h and appropriate secondary antibodies Alexa Fluor 488 or 568 (ThermoFisher) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The sections were blocked in 10% goat serum for 45 min and incubated with primary antibodies at 4°C overnight and secondary antibodies (Invitrogen) for 1 h at 37°C.
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bioRxiv - Immunology 2023Quote: ... the pellet was resuspended in 90 μl of Secondary antibody solution (Blocking buffer + 1:500 Goat anti-rabbit IgG AlexaFluor 647 antibody [#A27040, Invitrogen]) and the samples were incubated at room temperature for 45 min in the dark ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed in PBS and incubated in primary antibody solution containing astrovirus capsid monoclonal antibody 8e7 (Invitrogen MA5-16293) at 1:100 in 1% NGS/PBS for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Each sample was blocked with 3% BSA in PBS then stained anti-β (1,3)-glucan antibody (Biosupplies Australia Pty Ltd., Australia) followed by secondary antibody of anti-Mouse IgG (H+L) Alexa Fluor 488 (ThermoFisher). The level of β-1,3-glucan exposure on the cell surface was then quantified on a Beckman Coulter CytoFlex 3-laser cytometers ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibody incubations were done overnight at 4°C followed by PBS wash and Alexa Fluor secondary antibody (Life Technologies) incubations ...
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used here: Alexa Fluor 555-labeled donkey anti-rabbit secondary antibody (1:500, RRID: AB_162543, Invitrogen, USA), Alexa Fluor 488-labeled donkey anti-mouse secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then washed 3 times with Tween20 solution before a 1 h incubation with secondary antibody solution containing goat anti-mouse antibodies conjugated to AlexaFluor 647 (ThermoFisher), goat anti-rabbit antibodies conjugated to AlexaFluor 488 (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated in primary antibody diluted in blocking buffer using the following primary antibodies: anti-Phospho-Tau (Ser396) (1:500, 44-752G, Invitrogen), anti β-catenin (E-5 ...