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Citations for Thermo Fisher :
5101 - 5150 of 10000+ citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... The cells were washed three times in PBS and incubated with the secondary antibody for 1 hour at room temperature (goat anti-rabbit antibody conjugated with AlexaFluor488 (Invitrogen); diluted 1:200 in blocking reagent ...
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bioRxiv - Neuroscience 2022Quote: ... Slices from the microinjection experiments were additionally incubated with primary antibody Chicken IgY GFP Polyclonal Antibody (1:100, ThermoFisher, #A10262) for 48 h at 4 °C and with secondary antibody Goat anti-Chicken IgY (H+L ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting bands were visualized with a secondary antibody Anti-rabbit IgG HRP-linked antibody (Thermo Fisher Scientific, Waltham, MA) at 1:1000 for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... and 0.2% Triton X-100 at RT for 1 h, and then blocked with an anti-dsRNA antibody (Scicons, 10010200) and a fluorescent secondary antibody (Invitrogen). Images were captured with the CellInsight CX7 High-Content Screening (HCS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... protein was detected using a specific antibody (1:1500, Sinobiological) and an Alexa Fluor 488 anti-rabbit secondary antibody (1:200, Invitrogen). The nucleus was labeled using DAPI (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... HTM cells were stained with an anti-paxillin primary antibody followed by incubation with an Alexa Fluor-488 fluorescent secondary antibody and Hoechst 33342 counterstain (all from Invitrogen) to reveal focal adhesions and nuclei ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the secondary antibody was a 1/10,000 diluted polyclonal goat anti-mouse Alexa-Fluor 680 fluorophore-conjugate fluorescent secondary antibody (Thermo Fisher).
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bioRxiv - Microbiology 2019Quote: ... and subsequently stained with a primary murine anti-IE1 antibody and a secondary Alexa 647 Goat anti-mouse antibody (Thermofisher). Nuclei were additionally stained with Hoechst 33342 (Thermofisher) ...
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bioRxiv - Physiology 2019Quote: ... Primary antibody was detected by a 1h incubation with AlexaFluor-conjugated goat anti-rabbit secondary antibody (Life Technologies, A-11072) diluted in blocking buffer ...
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bioRxiv - Microbiology 2019Quote: ... the membrane was probed with HIV-Ig as the primary antibody and corresponding secondary antibody conjugated to horseradish peroxidase (goat anti-human IgG, Invitrogen). Gag-specific bands were detected after incubation with SuperSignal West Pico chemiluminescent substrate (Pierce ...
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bioRxiv - Genetics 2019Quote: ... for 3 min and incubated with Alexa647-conjugated anti-Kv2.1 mouse monoclonal antibody (K89/34, Neuromab, Antibodies Inc., Davis, CA; A20186 Thermo Fisher; 1:200 dilution in Perm buffer with 3% NGS ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibodies were detected with peroxidase conjugated goat secondary antibody and enhanced chemiluminescence (ECL) western blotting detection reagent (Thermo Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... Similar expression levels between extracts were verified by dot blot using a Cas9 antibody (Cas9 Monoclonal Antibody 7A9, Thermo Fisher). A bacterial extract of N57 was used as a positive control ...
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bioRxiv - Microbiology 2019Quote: ... rabbit anti-MreBCT antibody (custom anti-peptide antibody directed against the C-terminus of C. trachomatis serovar L2 MreB; ThermoFisher), rabbit anti-Hsp60 (kind gift of Dr ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated with appropriate primary antibodies followed by staining with ALEXA FLUOR-488/594 donkey or rabbit anti-mouse secondary antibodies (Invitrogen). The cells were washed 4 times and a final concentration of 0.1 μg/ml DAPI (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies in 2% BSA/PBS were incubated overnight at 4°C and secondary antibodies (Thermo Fisher Scientific, Alexa Fluor) were incubated for 2 hours at room temperature and washed with PBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... or donkey anti-goat 568 Alexa Fluor-conjugated secondary antibodies (diluted 1:1,000 in antibody dilution buffer; Thermo-Fisher Scientific) were applied at room temperature for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... 1% [v/v] FCS in PBS++) and primary antibodies were detected with secondary antibodies conjugated to Alexa Fluor dyes (Invitrogen). Images were acquired as above ...
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bioRxiv - Molecular Biology 2020Quote: ... The sections were washed with Antibody diluent and a secondary antibody (anti-Mouse AlexaFluor 488 (Thermo Fisher Scientific, 1:1000)) was applied for 1 hour at room temperature in a humidified chamber ...
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bioRxiv - Neuroscience 2019Quote: ... Tyrosine hydroxylase immunoreactions (primary antibody: Chicken anti-TH, 1:500 Abcam; secondary antibody: Alexa Fluor 488 1:1000, Life Technologies) were carried out in 3 or more striatal and at least 1 nigral section ...
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bioRxiv - Neuroscience 2020Quote: ... (see Table S4 for primary antibodies) and donkey anti Rabbit or mouse AlexaFlour 488 or 555 secondary antibodies for 1 hr (Thermofisher). Nuclei were visualized using DAPI (2 μg/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were then washed 3 times in washing solution before being incubated with secondary antibodies (see Table S4 for secondary antibodies used and their dilution) and DAPI at 4 μg/mL (Invitrogen) in blocking solution for 1 h ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and then stained with HERV-K envelope primary antibodies (1:1000) (Ango) overnight at 4°C and Alexa Fluor 488 secondary antibodies (1:500) (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... blocked and incubated with Anti-Loqs or anti-NS3 primary antibodies at a dilution of 1:200 followed by incubation with Alexa-Fluor 488 secondary antibodies (Invitrogen) at a dilution of 1:500 ...
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bioRxiv - Developmental Biology 2019Quote: ... Primary antibodies were detected using secondary antibodies: goat anti-mouse Alexa 564 or goat anti-rabbit Alexa 488 (Life Technologies). Nuclei were labeled with Dapi ...
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bioRxiv - Biochemistry 2019Quote: ... Primary antibody immunolabelling was carried out for 1hr at rt with 1:2000 diluted FLAG tag mouse monoclonal antibody (FG4R, ThermoFisher). After incubation with the secondary alkaline phosphatase-conjugated goat Anti-Mouse IgG (H+L ...
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bioRxiv - Physiology 2019Quote: ... Samples were probed with a primary antibody against MCT4 (1:1000; AB3314P, Merck Millipore and AlexaFluor488 secondary antibody (1:2000; A11008, Invitrogen). Nuclei were stained with 4,6-diamidino-2-phenylindole (1:50,000 ...
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bioRxiv - Cell Biology 2019Quote: Activation of β1 integrin was assessed by immunofluorescent staining using 9EG7 or 12G10 antibodies and AlexaFluor488-conjugated secondary antibodies (Invitrogen). Imaging was performed using a fluorescence microscope (Olympus ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated for 1 h with primary/detection SARS-CoV-2 anti-RBD rabbit polyclonal antibody (Sinobiologicals) and then 1 h with secondary anti-rabbit HRP-conjugate antibody (Invitrogen). Foci were visualised using TrueBlue™ Peroxidase Substrate (Sera Care ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in 5% BSA in TBS-T for 30min before incubated with specific primary antibodies and the corresponding secondary antibody (Invitrogen, #A21206 and #A10038 ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... Fixed cells were incubated with anti-MyHC antibodies (MF20) overnight at 4°C and then incubated with anti-mouse secondary antibodies labeled with Alexa fluor 594 (Invitrogen) for 1 hour at room temperature and counterstained with DAPI ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated with primary antibodies overnight at 4°C using an antibody against V5 (mouse monoclonal R960-25; 1:1000; Invitrogen), spike (mouse monoclonal GTX632604 ...
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bioRxiv - Immunology 2021Quote: For the antibodies, plasmids encoding heavy and light chains of antibodies (CR3022, and SR1-SR5) were co-transfected into Expi293F cells (ThermoFisher) according to the manufacturer’s instructions for expression of antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... followed by a 2 hour room temperature incubation in 10% NGS/90% antibody buffer containing the following fluorescently-conjugated secondary antibodies: Goat anti-mouse Alexa 488 (1:500; Invitrogen) and goat anti-rabbit Alexa 594 (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... The membranes were blocked with 5% fat-free milk and thereafter probed with primary antibodies 6xHis Tag Monoclonal Antibody (Invitrogen) followed by incubation with horseradish peroxidase-labeled secondary antibody ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary staining was performed with goat antibodies conjugated to Alexa Fluors 488 and 555 targeting the primary antibody species (1:200, Invitrogen).
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bioRxiv - Genetics 2019Quote: ... samples were washed three times in PBT for 15 min and incubated for 2 h at room temperature with secondary antibody (antibodies coupled to Alexa fluorophores, Invitrogen) diluted 1:600 in PBT containing Hoechst dye ...
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bioRxiv - Molecular Biology 2020Quote: ... To measure colocalization of TCRA and TCRB mRNAs with each other or with P bodies (using DCP1 antibody) or stress granules (G3BP1 antibody) the cells were mounted along with 0.1 µm TetraSpeck™ microspheres (ThermoFisher) adhered to the slide according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Nunc MaxiSorp ELISA plates were coated overnight at 4°C with 50 ml of capture antibody (anti-6xHis antibody, clone HIS.H8; ThermoFisher Scientific) at 2 mg/mL in PBS ...
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bioRxiv - Immunology 2021Quote: ... as the primary antibody and a 1:4,000 dilution of goat anti-mouse alkaline phosphate conjugate as the secondary antibody (ThermoFisher Scientific). Each dilution was tested in triplicate and neutralization curves were graphed using GraphPad Prism.
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bioRxiv - Immunology 2021Quote: ... tissue sections were washed 3 times with PBS-T and the following secondary antibodies were used to detect the primary antibodies: Streptavidin-AF555 (Invitrogen), anti-rat IgG2a-AF488 (SouthernBiotech) ...
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bioRxiv - Developmental Biology 2021Quote: ... the sections were incubated in secondary antibodies (Alexa Fluor 488-, 555-, 594-, 647-conjugated secondary antibodies, 1:500; Life Technologies, Carlsbad ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody labelling was performed with primary anti-ACTN4 (rabbit; ENZO) and secondary anti-rabbit-AlexaFluor488 antibody (goat; Thermo Fisher Scientific). Nuclear compartment was labelled with DAPI ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibodies used: F(ab’)2-Donkey anti-Goat IgG cross absorbed PE secondary antibody (Thermo Fisher Scientific Cat: 31860), anti-mouse APC secondary antibody (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Proteins were detected on a Li-Cor Odyssey Blot Imager using an anti-Caspase-11 primary antibody (cone 17D9) and Alexfluor-680 conjugated secondary antibody (Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... equilibriated in 1X PBS were used to coat with anti-TLK1 or IgG isotype antibody (0.5μg antibody per reaction, Thermofisher, cat# 720397). To ensure the unfirom coating of beads ...
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Synthetic lethal targeting of TET2-mutant hematopoietic stem and progenitor cells by XPO1 inhibitorsbioRxiv - Cancer Biology 2022Quote: ... were co-incubated at 4°C overnight and visualized with Alexa Fluor 488 goat anti-mouse secondary antibody and Alexa Fluor 546 goat anti-rabbit secondary antibody (Invitrogen). TUNEL apoptosis assay was performed on embryos with the In-Situ Cell Death Detection kit (POD ...
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bioRxiv - Cancer Biology 2022Quote: ... Clarified lysates were incubated with 1 ug of YB-1 antibody and antibody/protein/RNA complexes were isolated by using 20 μl packed volume protein A sepharose beads (Invitrogen). Beads were washed with polysome extraction buffer and RNA isolated using the PeqGold RNA isolation kit ...
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bioRxiv - Cell Biology 2022Quote: ... LiCor Biosciences) and probed with primary antibodies listed above and goat secondary antibodies conjugated with Alexa Fluor 680 or 800 (Invitrogen) for detection on an Odyssey infrared imaging system (LiCor Bioscience).
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bioRxiv - Developmental Biology 2022Quote: ... whereas for mouse monoclonal primary antibodies, we used isotype-specific Alexa-conjugated Goat secondary antibodies (against IgG1, IgG2a; IgG2b) from Thermofisher scientific.