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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cell culture supernatants were collected at the end of culture and analyzed for IL-2 by ELISA using a kit (cat # 88-7025-76) purchased from Thermofisher Scientific.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The membranes were then treated with secondary antibodies (biotinylated goat anti-rabbit IgG (#65-6140, Invitrogen, Rockford, IL, United States), biotinylated goat anti-rat IgG (#405428 ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the supernatant for immunoprecipitation was measured via the bicinchoninic acid assay (Thermo Scientific, Rockford, IL, USA) (Walker ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse IgGs were stained with Alexa Fluor 568 conjugated donkey anti-mouse IgG (Invitrogen, Cat#A-10037). Panleucocytes were identified using rat anti-mouse CD45 antibodies (BD Pharmingen Inc ...
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bioRxiv - Neuroscience 2021Quote: ... biotinylated horse anti-mouse IgG (H+L) and biotinylated goat anti-mouse IgG2a (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... biotinylated horse anti-mouse IgG (H+L) and biotinylated goat anti-mouse IgG2a (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
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bioRxiv - Molecular Biology 2020Quote: ... and mouse antibodies were coupled to Dynabeads coated with Pan Mouse IgG antibodies (Thermo Fisher Scientific, 11042). 50 μL of the appropriate Dynabeads pre-coupled with the indicated antibody were added to the chromatin sample and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... goat anti-mouse Alexa Fluor 568 or goat anti-mouse Alexa Fluor 488 secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Mouse monoclonal antibodies were detected with a goat anti-mouse IgG HRP-linked antibody (62-6520; Invitrogen) diluted 1:10,000 (v/v) ...
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bioRxiv - Immunology 2020Quote: ... Isotype control and mouse monoclonal antibody to mouse IFNβ (Clone: MAR1-5A3) were purchased from Thermo scientific. SP600125 and C-16 purchased from Calbiochem ...
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bioRxiv - Microbiology 2022Quote: ... CA) or Alexa Fluor 488 anti-mouse antibody (for LP11 and mouse anti-myc; Invitrogen, Waltham, MA) at a 1:250 dilution in FACS medium ...
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bioRxiv - Neuroscience 2024Quote: ... BV-2 cells were fixed and sequentially incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) and Navenci blocking solution at 37°C for 60 min each in a pre-heated humidified chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse IL21 protein levels in BM supernatant were determined using an IL21 Mouse ELISA kit (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor 488 goat anti-mouse and anti-rabbit IgG (Invitrogen, Waltham, MA; catalog # A-11001 (mouse) and A-11008 (rabbit) ...
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bioRxiv - Neuroscience 2023Quote: Mouse samples: total RNA from mouse blood samples was extracted using the RiboPure RNA Purification Kit (Invitrogen). Total RNA from brain regions was extracted ...
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bioRxiv - Neuroscience 2024Quote: ... and 647 (1:500 for all; PIA32773-mouse; A21202-mouse; PIA32731TR-rabbit; PIA32794-rabbit; A21448-sheep, Invitrogen). The sections were examined using an AX-R Confocal Microscope coupled to a fully automated Ti2-E system (Nikon Instruments Inc ...
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bioRxiv - Microbiology 2024Quote: ... and amastigotes were labeled with mouse anti-P8 and A488-conjugated goat anti-mouse secondary antibody (Invitrogen); samples were co-stained with DAPI ...
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bioRxiv - Microbiology 2024Quote: ... and amastigotes were relabeled with mouse anti-P8 and A488-conjugated goat anti-mouse secondary antibody (Invitrogen); samples were co-stained with DAPI ...
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bioRxiv - Genetics 2019Quote: ... Thirty-two libraries of 100 pM were mixed together and emulsion PCR amplified on the Ion PI™ Hi-Q™ ISPs using the Ion OneTouch™ 2 Instrument (Life Technologies, Carlsbad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Template preparation was performed by the Ion PGM Hi-Q View kit on the Ion OneTouch™ 2 System (Life Technologies, Grand Island, NY, United States) and sequenced using the Ion PGM Hi-Q View sequencing kit (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... in HepG2 medium (Advanced MEM minus L-Glutamine [Gibco] + 10% [v/v] HI-FBS [Gibco] + 1% [v/v] penicillin-streptomycin + 2 mM Glutamax [Gibco] and 0.1% [v/v] amphotericin B). Once the HepG2 or HC-04 cells were grown to the desired concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... blotted for 4 seconds at 4 °C in 100% humidity and plunge-frozen in liquid ethane by using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2019Quote: ... Collected samples were analyzed using native gel electrophoresis (NativePAGE 4-16% Bis-Tris Gel) or denature gel electrophoresis (SDS-PAGE, Novex 4-20% Tris-Glycine Mini Gels, Thermo Fisher Scientific). For SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... of HLC protein extract in 4 × Sample Loading Buffer (Li-Cor, 928-40004) were loaded onto NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen, NP0326BOX). Following resolution ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Samples equivalent to 100 μg of DNA were incubated overnight at 4°C with 4 μg of rabbit polyclonal anti-ATF7IP (Invitrogen, PA5-54811), rabbit monoclonal anti-JUNB (C37F9 ...
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bioRxiv - Biophysics 2020Quote: ... Grids were blotted for 2-4 seconds at a blotting force of 4 and plunge-frozen in liquid ethane using a MarkIV Vitrobot (Thermo Fisher Scientific). The chamber was maintained at 8 °C and 100% humidity during freezing ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We plated 4*105 cells/well of isolated cardiomyocytes to fibronectin (cosmo bio SFN01)-coated 4 well chamber slides (Thermo Fisher 154526) and cultured them in a 5% CO2 incubator ...
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bioRxiv - Developmental Biology 2022Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes (min ...
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bioRxiv - Microbiology 2020Quote: ... Fungal hyphae were stained with 0.5 mM 4.4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (C1-BODIPY 500/510 C12, Thermo Fisher Scientific) or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16 ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was kept after spinning at 800 rcf for 2’ at +4°C and were incubated overnight at +4°C with 3µg of dedicated antibodies: Nr2f1 antibody (Thermo Fisher PA5-21611) and GFP antibody (Abcam ab13970 ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were centrifuged at 17,800 × g for 5 min at 4°C and supernatants were mixed with NuPAGE LDS Sample Buffer (4×) buffer (Thermo Fisher Scientific) containing 1% (v/v ...
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bioRxiv - Immunology 2020Quote: ... 2-4 × 105 cell equivalents per well were loaded into a NuPAGE 4-12% Bis-Tris density gradient gel (Thermo Fisher Scientific) and ran at a constant 150V for 80 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were loaded with the calcium indicator Fluo-4 by incubating in supplemented NB medium that contained 2 µM of Fluo-4 AM (Invitrogen, Carlsbad, CA), and Pluronic F-127 (0.04% ...
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bioRxiv - Cell Biology 2020Quote: Cells were plated at a density of 4 × 104 cells per well in a 4 well chamber slide (Nunc Lab-Tek II Chamber slide system, Thermo Fisher Scientific), and cultured in the 20 mM sodium acetate-supplemented culture medium (10% FBS) ...
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bioRxiv - Biochemistry 2021Quote: Images displayed in Figure 4 and negative stain images in supplementary Figure 4 were acquired using a Tecnai TF20 electron microscope (Thermo Fisher, USA) equipped with FEG source operating at 200 keV ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified eIF1A derivatives were labeled at 4 °C with 4-fold excess of Alexa Fluor™ 555 C2 maleimide (Alexa555, Thermo Scientific) following the manufacturer’s manual ...
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bioRxiv - Immunology 2022Quote: ... or corresponding isotype control antibodies diluted in FACS buffer for 30 min at 4 ° After fixation and permeabilization for 30 min at 4 °C using the Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific), resuspended cells were kept overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the grid was blotted for 4 sec under 100% humidity at 4°C and then plunged into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2023Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ... the C-terminal FLAG-tagged WT sCTLA-4 and Y139A sCTLA-4 in pCMV-Tag4A were sub- cloned into the pFastBac vector (Thermo Fisher Scientific) to generate pFastBac-WT_sCTLA- 4-FLAG and pFastBac-Y139A_sCTLA-4-FLAG.
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bioRxiv - Neuroscience 2023Quote: ... The neuronal cultures were washed twice for 10 minutes with HBSS and then loaded with 4 μM of Fluo-4 AM (Molecular probes, F14201) in HBSS having 0.002% Pluronic F-127 for 30 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Then the cells were stained with a solution of 4 mM calcein AM (TRC, #C125400) and 4 mM ethidium homodimer-1 (Thermo Fisher, #L3224) dissolved in PBS to identify live and dead cells ...
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bioRxiv - Molecular Biology 2023Quote: ... 3D artificial muscles were fixed with buffered 1% paraformaldehyde (PFA) (ThermoScientific, JI9943.K2) overnight at 4°C followed by 6 hours of blocking at 4°C [10% FBS (Gibco, 10270-106), 1% BSA (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The pellets were washed twice and cell surface AF488 fluorescence was quenched by incubation for 30 min at 4 °C with rabbit anti-AF488 IgG (4 µg/mL, Thermo Fisher Scientific). The difference between the signals from the cells incubated in the absence and presence of anti-AF488 IgG corresponded to apoA-I bound to the cell surface.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were centrifuged at 20,800 × g for 5 min at 4°C and supernatants were mixed with NuPAGE LDS Sample Buffer (4×) buffer (Thermo Fisher Scientific). For SDS-PAGE ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... and the airway and vessel-free parenchyma was further minced 4 x 4 mm pieces and cryopreserved in high glucose DMEM GlutaMAXTM (Thermo Fisher Scientific) containing 10% DMSO (Carl Roth ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μl of this mixture was loaded into the lanes of a precast 4-12% gradient polyacrylamide gel (4–12% Bolt Bis-Tris Plus Gels, Thermo Fisher Scientific).
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bioRxiv - Genetics 2024Quote: Cells were grown on 1.5mm coverslips coated with matrigel (iMGLs). Cells were first fixed in a 4% formaldehyde (Electron Microscopy cat. # 15714) solution in 4% sucrose in PBS (Thermo Fisher Scientific) for 10 minutes at room temperature ...